Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,836,397	G→A	E109K (GAG→AAG)	ynjB →	putative ABC transporter periplasmic binding protein YnjB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,836,397	0	G	A	100.0%	214.4 / NA	61	E109K (GAG→AAG)	ynjB	putative ABC transporter periplasmic binding protein YnjB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/38);  total (23/38)

CTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTATGCTGACGC  >  NC_000913/1836109‑1836688                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    cTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATtta                                                                                                                                                                                                                                                                                          >  1:91193/1‑300 (MQ=255)   gTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAANCGCTATCTCGACTGGGTGAGCGGCGAGATGAANACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATttatt                                                                                                                                                                                                                                                                                        >  2:541257/1‑300 (MQ=255)                   caTGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGCTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAAGCCAATTTATTACAAACGGGCTGGGc                                                                                                                                                                                                                                                                         >  2:633891/1‑299 (MQ=255)                         aGTGACTTGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGTGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGgaga                                                                                                                                                                                                                                                                    <  2:179459/298‑1 (MQ=255)                         aGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTTAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGNCGCGGTGAAGCGCATTCAGACCGAAGCCGCAGNCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGAc                                                                                                                                                                                                                                                                   <  2:542815/299‑1 (MQ=255)                         aGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCTGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGAc                                                                                                                                                                                                                                                                   <  1:275976/299‑1 (MQ=255)                         aGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGgaga                                                                                                                                                                                                                                                                    <  1:100521/298‑1 (MQ=255)                         aGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGAc                                                                                                                                                                                                                                                                   <  1:180569/299‑1 (MQ=255)                                   aaGAAATTAAAAATTAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAACACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAAc                                                                                                                                                                                                                                                         <  2:632546/299‑1 (MQ=255)                                   aaGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAAc                                                                                                                                                                                                                                                         <  1:299798/299‑1 (MQ=255)                                      aaaTTAAAAAGGAGGACAAAAGGCAAACCGTCTGGTTTAACGCCTTGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGGGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTTGCGGATGCCGCAGACGCGGTGAAACGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGACTCGGTGGATCTGCTCTGGGTGAACGGCGAAGACTTCCGCACCTTAAAAAAGGCCAATTTATTACGAACGGGCTGGTCGGAGAc                                                                                                                                                                                                                                                                   <  2:371789/286‑1 (MQ=255)                                             aaaTGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGt                                                                                                                                                                                                                                              <  1:393176/300‑1 (MQ=255)                                                  aGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGaca                                                                                                                                                                                                                                         <  1:545316/300‑1 (MQ=255)                                                    gCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTTAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTCGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGacaca                                                                                                                                                                                                                                       >  1:421421/1‑300 (MQ=255)                                                     ccAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGacacac                                                                                                                                                                                                                                      >  1:339797/1‑300 (MQ=255)                                                        aaGGGCAAGCCGTTTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGTCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGc                                                                                                                                                                                                                                   <  2:146677/300‑1 (MQ=255)                                                        aaGGGCAAACCTTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGc                                                                                                                                                                                                                                   <  2:105730/300‑1 (MQ=255)                                                          gggCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCt                                                                                                                                                                                                                                  >  1:644443/1‑299 (MQ=255)                                                                 ccGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGc                                                                                                                                                                                                                          >  1:486808/1‑300 (MQ=255)                                                                   gTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGc                                                                                                                                                                                                                          >  2:478633/1‑298 (MQ=255)                                                                                         gcgATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCTAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGTGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGacac                                                                                                                                                                                                  <  2:91193/300‑1 (MQ=255)                                                                                         gcgATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGacac                                                                                                                                                                                                  >  1:698113/1‑300 (MQ=255)                                                                                             tACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAgg                                                                                                                                                                                              <  1:585744/300‑1 (MQ=255)                                                                                                    aTTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGTCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGa                                                                                                                                                                                        >  1:175893/1‑299 (MQ=255)                                                                                                     ttAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAAt                                                                                                                                                                                      <  2:290040/300‑1 (MQ=255)                                                                                                      tAACCGCTATCTCGACTGGGTGAGCTGCTAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTANAACGGGCTGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCg                                                                                                                                                                                          <  2:233795/295‑1 (MQ=255)                                                                                                           gCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGccc                                                                                                                                                                                 <  1:333056/299‑1 (MQ=255)                                                                                                               tctcGACTGGGTGAGCGGCTAGGTGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTCGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTgggg                                                                                                                                                                            <  2:592123/300‑1 (MQ=255)                                                                                                                 tcGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCAGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCTGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGggc                                                                                                                                                                           <  1:698017/299‑1 (MQ=255)                                                                                                                 tcGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCAGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGggc                                                                                                                                                                           <  1:698011/299‑1 (MQ=255)                                                                                                                  cGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGTGGGGAGATTTTTCTGTGCCGACAAAAGGTGCGGAATCGCACTGGggc                                                                                                                                                                           >  2:149260/1‑298 (MQ=255)                                                                                                                          tGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAAc                                                                                                                                                                 <  1:477721/300‑1 (MQ=255)                                                                                                                            aGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTg                                                                                                                                                               <  1:131157/300‑1 (MQ=255)                                                                                                                                        aaaaCCCATTACGCTATAAGCCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGTGCTGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGTAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATcgcc                                                                                                                                                   <  2:167562/300‑1 (MQ=255)                                                                                                                                                          aaCCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCTGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACg                                                                                                                                                            <  2:138708/273‑1 (MQ=255)                                                                                                                                                          aaCCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTNTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACg                                                                                                                                                            >  1:138708/1‑273 (MQ=255)                                                                                                                                                                    ttGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGGAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCcaccac                                                                                                                       <  1:446503/300‑1 (MQ=255)                                                                                                                                                                     tGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACcaca                                                                                                                      <  1:590502/300‑1 (MQ=255)                                                                                                                                                                                      ccGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTAc                                                                                                     >  1:664605/1‑300 (MQ=255)                                                                                                                                                                                        gCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACt                                                                                                    <  1:30579/299‑1 (MQ=255)                                                                                                                                                                                               cgGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGACCGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTNCCGGTGTGGGAAGATTTTTCTGTGCAGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGAGGTTAAGCCACAGCCACCACAAACACCGCAAGCAGTAATGGAGttt                                                                                             >  2:235855/1‑299 (MQ=255)                                                                                                                                                                                                 gTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGAACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCAACAGCAAACACAAACGCCGCAAGCCTTAATGGATTTTGCt                                                                                          >  2:725090/1‑300 (MQ=255)                                                                                                                                                                                                      gcgcATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGc                                                                                     <  2:422914/300‑1 (MQ=255)                                                                                                                                                                                                         cATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCaa                                                                                  >  1:565116/1‑300 (MQ=255)                                                                                                                                                                                                                         gCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTACTAAAGCCAATCCCGGCACGGTTAcc                                                                  <  1:218462/300‑1 (MQ=255)                                                                                                                                                                                                                           agctggACGTAAAACGGGCGGCCCGTTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGTCGGAGACTCTGTCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACGGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTGGCTAAAGCCAATCCCGGCACGGTTACCt                                                                 <  2:180787/295‑1 (MQ=255)                                                                                                                                                                                                                               ggACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATc                                                              <  2:499886/298‑1 (MQ=255)                                                                                                                                                                                                                                    tAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGccc                                                       <  1:546336/300‑1 (MQ=255)                                                                                                                                                                                                                                           ggcggcTCGGTGGATCTGCTCTGGGTGAACTGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGAc                                                <  2:717467/300‑1 (MQ=255)                                                                                                                                                                                                                                               gcTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTa                                            >  1:241889/1‑300 (MQ=255)                                                                                                                                                                                                                                                       gATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGGAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCa                                      >  2:21267/1‑298 (MQ=255)                                                                                                                                                                                                                                                       gATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCAGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCAc                                     >  1:396172/1‑299 (MQ=255)                                                                                                                                                                                                                                                            gCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGtt                               >  1:649118/1‑300 (MQ=255)                                                                                                                                                                                                                                                            gCTCTAGGTGAACGGCGAAAACTTCCGCACCTTAAAAAAGACCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGAGATGTTACGCCACAGCCAACACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGACAAt                                                                                 >  2:282686/1‑250 (MQ=255)                                                                                                                                                                                                                                                                    tGAACGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGTGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCTGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGGTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTATCTGCACGGCGTTTCTTGAAc                       <  2:483650/300‑1 (MQ=255)                                                                                                                                                                                                                                                                      aaCGGCGAAAACTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAg                     <  1:631052/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              aaaCTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGAt             <  1:253632/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                 cTTCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTAt          >  1:701701/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   tCCGCACCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTATGc        <  1:276328/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                        aCCTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTATGCTGACg   <  1:541257/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                         ccTTAAAAAAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTATGCTGACGc  >  1:288489/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    CTGTTTTCTCTGGCAACACATGCCAGTGACTGGCAAGAAATTAAAAATGAGGCCAAAGGGCAAACCGTCTGGTTTAACGCCTGGGGCGGCGATACCGCAATTAACCGCTATCTCGACTGGGTGAGCGGCGAGATGAAAACCCATTACGCTATAAACCTGAAGATTGTCCGTCTGGCGGATGCCGCAGACGCGGTGAAGCGCATTCAGACCGAAGCCGCAGCCGGACGTAAAACGGGCGGCTCGGTGGATCTGCTCTGGGTGAACGGCGAAAACTTCCGCACCTTAAAAGAGGCCAATTTATTACAAACGGGCTGGGCGGAGACTCTGCCCAACTGGCGCTATGTCGACACACAGCTGCCGGTGCGGGAAGATTTTTCTGTGCCGACACAAGGTGCGGAATCGCCCTGGGGCGGCGCACAACTGACGTTTATCGCCCGCCGCGATGTTACGCCACAGCCACCACAAACGCCGCAAGCCTTACTGGAGTTTGCTAAAGCCAATCCCGGCACGGTTACCTATCCGCGCCCACCGGACTTTACCGGCACGGCGTTTCTTGAACAGTTGCTGATTATGCTGACGC  >  NC_000913/1836109‑1836688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑