breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 40 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,037,4960AT48.6% 51.2 / 294.0 147intergenic (+161/‑23)alaT/rrlAtRNA‑Ala/23S ribosomal RNA
*NC_00091358,0650CA37.4% 136.4 / 22.2 99P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_000913320,8450CA36.7% 86.9 / 12.3 79R207R (CGG→AGG) rclRDNA‑binding transcriptional activator RclR
*NC_0009132,073,6130GT35.9% 118.2 / 10.9 78R692L (CGG→CTG) fluself recognizing antigen 43 (Ag43) autotransporter
*NC_0009133,669,7150GT35.1% 153.2 / 15.3 111E42* (GAG→TAG) treFcytoplasmic trehalase
*NC_0009131,351,4630CA35.1% 80.9 / 11.4 77E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009132,288,4180GT33.9% 187.7 / 16.7 118pseudogene (497/2525 nt)yejOadhesin‑like autotransporter YejO
*NC_0009133,911,5330CA33.3% 171.7 / 15.4 114intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_00091385,6510GT32.3% 185.0 / 13.5 130E8* (GAG→TAG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
*NC_0009132,427,7160CA31.1% 222.0 / 10.4 106E26* (GAG→TAG) argTlysine/arginine/ornithine ABC transporter periplasmic binding protein
*NC_0009131,272,4200CA31.1% 157.5 / 16.4 103intergenic (+71/‑87)chaB/chaCputative cation transport regulator ChaB/glutathione‑specific gamma‑glutamylcyclotransferase
*NC_000913442,0250CA30.0% 159.4 / 11.1 90L159L (CTC→CTAthiItRNA uridine 4‑sulfurtransferase
*NC_0009131,502,7700TC29.9% 165.4 / 36.9 107I105T (ATA→ACA) ydcLDUF3313 domain‑containing lipoprotein YdcL
*NC_0009132,767,4440CA29.7% 284.6 / 21.1 128intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,767,4470CA29.3% 313.1 / 15.8 133intergenic (+92/‑263)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_000913141,8490CA29.1% 215.6 / 10.2 117P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_0009134,540,4110CA28.4% 306.0 / 14.2 169intergenic (‑910/‑546)nanC/fimBN‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
*NC_000913848,0280GT27.8% 217.7 / 21.9 126intergenic (‑24/+380)glnH/dpsL‑glutamine ABC transporter periplasmic binding protein/DNA protection during starvation protein
*NC_0009132,412,3960CA27.7% 211.3 / 11.0 112E67* (GAG→TAG) yfbUPF03887 family protein YfbU
*NC_0009132,465,0160CA27.7% 218.2 / 10.3 112intergenic (‑9/‑285)mlaA/yfdCintermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA/inner membrane protein YfdC

Marginal new junction evidence (lowest skew 10 of 21 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2357730112 (1.230)10 (0.130) 9/510 5.2 9.4% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 95 (1.200)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 3648094100 (1.090)11 (0.120)
+T
11/588 5.3 9.6% noncoding (32/82 nt) agrA small RNA AgrA
?NC_000913 3648327 = 108 (1.180)noncoding (34/82 nt) agrB small regulatory RNA AgrB
* ? NC_000913 = 326989999 (1.080)9 (0.100) 8/554 6.0 8.7% intergenic (+297/+317) yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
?NC_000913 3270239 = 97 (1.130)noncoding (354/377 nt) rnpB RNase P catalytic RNA component
* ? NC_000913 = 128690645 (0.490)9 (0.110) 7/550 6.4 16.8% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 47 (0.550)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 69650696 (1.050)4 (0.060) 4/436 6.6 5.5% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 696619 = 67 (0.990)intergenic (‑3/+45) glnV/metU tRNA‑Gln/tRNA‑Met
* ? NC_000913 = 265341986 (0.940)5 (0.060) 5/540 7.2 6.1% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 76 (0.910)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 252776101 (1.110)7 (0.080) 6/590 7.2 6.5% coding (68/453 nt) yafP putative N‑acetyltransferase YafP
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 128708449 (0.540)7 (0.080) 5/550 7.3 13.1% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 47 (0.550)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 429587089 (0.970)4 (0.050) 4/512 7.5 5.5% intergenic (+76/+566) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
?NC_000913 4296434 = 59 (0.740)intergenic (+640/+2) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
* ? NC_000913 = 78145165 (0.710)5 (0.060) 5/582 7.6 6.9% intergenic (+7/‑126) lysZ/lysQ tRNA‑Lys/tRNA‑Lys
?NC_000913 781660 = 71 (0.790)intergenic (+8/‑425) lysQ/nadA tRNA‑Lys/quinolinate synthase