Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,541,037	A→G	I518I (ATT→ATC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,541,037	0	A	G	100.0%	213.4 / NA	58	I518I (ATT→ATC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/35);  total (23/35)

GGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACC  >  NC_000913/1540742‑1541330                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      ggCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGatat                                                                                                                                                                                                                                                                                                   >  1:507417/1‑300 (MQ=255)    tGGAATGATTGTAGAAAAACTAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCTCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATAc                                                                                                                                                                                                                                                                                                 <  2:291620/299‑1 (MQ=255)    tGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCNACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATAc                                                                                                                                                                                                                                                                                                 <  1:529687/299‑1 (MQ=255)    tGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATAc                                                                                                                                                                                                                                                                                                 <  1:24034/299‑1 (MQ=255)      gAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACt                                                                                                                                                                                                                                                                                                >  2:412615/1‑298 (MQ=255)                             gCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTc                                                                                                                                                                                                                                                                       <  1:428057/300‑1 (MQ=255)                                       ttgcgcGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  1:67250/298‑1 (MQ=255)                                       tgtcgcgGTGTTCGTTTCCAGTCGATCGCAAAGGCCAGTTGCAACCAGCCGGTTTGTGTGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGTGCGGTATCGGCAAATTCACGTGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  2:191003/297‑1 (MQ=255)                                       tggtgcgGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGTGATGGTTTCGATATACTGGCGCGGCACGCCAGTAGTTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  2:418846/296‑1 (MQ=255)                                       tGGCGCGGTGGTCGTTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  2:480779/300‑1 (MQ=255)                                       tGGCGCGGTGGTCGTTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCAGGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCATGCt                                                                                                                                                                                                                                                             <  1:566075/300‑1 (MQ=255)                                       tGGCGCGGTGGTCGGTTCCAGTCGAGCGCAGAGGCCAGTGGCAACCAGCCGGTTTGTGTGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  2:418859/300‑1 (MQ=255)                                       tGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGTGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  1:68273/300‑1 (MQ=255)                                       tGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  1:67559/300‑1 (MQ=255)                                       tGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGGAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCt                                                                                                                                                                                                                                                             <  2:114921/300‑1 (MQ=255)                                             ggtggtCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGTGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGgt                                                                                                                                                                                                                                                        <  2:649741/299‑1 (MQ=255)                                                ggttggTTCCAGTCGAGCGCAAAGTCCAGTGGCAACCAGCCGGTTTGGGGGCGCAGTTTTTCCTTGCTGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTTGATATACTGGCGCGGCACGCCAGTAATTGGCTCACCCCAGGCTTGGGTGTAc                                                                                                                                                                                                                                                    <  1:300555/296‑1 (MQ=255)                                                ggtCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTAc                                                                                                                                                                                                                                                    <  1:300568/300‑1 (MQ=255)                                                    gtttCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGt                                                                                                                                                                                                                                                 <  2:657333/297‑1 (MQ=255)                                                    ggTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGt                                                                                                                                                                                                                                                 <  1:505940/299‑1 (MQ=255)                                                     gTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGttt                                                                                                                                                                                                                                               >  1:470597/1‑300 (MQ=255)                                                           gTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGCAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc                                                                                                                                                                                                                                         >  2:470301/1‑300 (MQ=255)                                                           gTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACAACTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCCGTACTTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTc                                                                                                                                                                                                                                         >  2:345505/1‑300 (MQ=255)                                                                                      gCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATc                                                                                                                                                                                                              <  1:412615/300‑1 (MQ=255)                                                                                      gCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGAAACAACCGCAGAANATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATc                                                                                                                                                                                                              <  1:524030/300‑1 (MQ=255)                                                                                         ggTTTGTGTGCGCAGTTGTTCCTTGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGTGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATc                                                                                                                                                                                                              <  2:148889/297‑1 (MQ=255)                                                                                                    gcAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGAt                                                                                                                                                                                                <  2:604412/300‑1 (MQ=255)                                                                                                          tttCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTACAGCCCGCGATCGAGGc                                                                                                                                                                                          >  1:112933/1‑300 (MQ=255)                                                                                                                ggCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAAt                                                                                                                                                                                    >  1:360280/1‑300 (MQ=255)                                                                                                                         aGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACNTGCGCCGAGGATAATCATCGAGCGCCCATGCGNTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTa                                                                                                                                                                           >  2:539422/1‑300 (MQ=255)                                                                                                                              gCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCANGCTGGGGTGTACGGTTTGATTTAAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCaaa                                                                                                                                                                      >  2:574090/1‑300 (MQ=255)                                                                                                                                  aGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAAt                                                                                                                                                                     <  1:570752/297‑1 (MQ=255)                                                                                                                                         aCCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGc                                                                                                                                                            <  2:465088/299‑1 (MQ=255)                                                                                                                                            gCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTAGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGa                                                                                                                                                         >  1:225370/1‑299 (MQ=255)                                                                                                                                                 gCCCGACACAACCGCAGAAGATCAGCATATTAATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGATATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCCCCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCCTCCCGCCTGCGATCGAGGCCGTACTTCGCCAGTACCaa                                                                                                                                                                       >  2:159806/1‑280 (MQ=255)                                                                                                                                                                agaagaTCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCa                                                                                                                                     <  1:8760/299‑1 (MQ=255)                                                                                                                                                                agaagaGCAGCATATTGACCACCCCGCGGGAGTTCATGTCCACGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGcc                                                                                                                                      <  2:350244/298‑1 (MQ=255)                                                                                                                                                                    gaTCAGCATAGTGATCAGCCCGCGTTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGTCGAGGATAATCATCGATCGCCCATGCGTTTTATGGGCGGTATCGTCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGTGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTCCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGAc                                                                                                                                <  2:162555/300‑1 (MQ=255)                                                                                                                                                                     attaGCACATTGATCATCCCGCGGTAGGTCATGCCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGAGAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGTCAAATTCACGTGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCTTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGAc                                                                                                                                <  2:162493/296‑1 (MQ=255)                                                                                                                                                                           atatTGATCATCCCGCGGTAGTTCATGTCCATTTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGt                                                                                                                          <  2:593830/299‑1 (MQ=255)                                                                                                                                                                                               gTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAAtt                                                                                                      >  1:222701/1‑299 (MQ=255)                                                                                                                                                                                                 tCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTgg                                                                                                    <  1:361736/299‑1 (MQ=255)                                                                                                                                                                                                    tGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGANATACTGGCGCGGCACGCCAGTAATTTGCTCACNCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGgcgc                                                                                                 >  2:534582/1‑299 (MQ=255)                                                                                                                                                                                                         aTGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACAGGCAATTGGCGCACCAGc                                                                                           >  2:687781/1‑300 (MQ=255)                                                                                                                                                                                                                      ggTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGtt                                                                              >  1:73846/1‑300 (MQ=255)                                                                                                                                                                                                                      ggTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTCTTTAACGGGCAATTGGCGCACCAGCACCGAATtgtatt                                                                              >  2:279697/1‑297 (MQ=255)                                                                                                                                                                                                                       gTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGttt                                                                             >  1:617989/1‑300 (MQ=255)                                                                                                                                                                                                                          aaCACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTAc                                                                          <  1:502102/300‑1 (MQ=255)                                                                                                                                                                                                                                        ggATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGc                                                             >  1:258215/1‑299 (MQ=255)                                                                                                                                                                                                                                               catcGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGTTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGTACAGGTGTTGCCATCGACTAACGTAAGGTTTTTAACGGGCAATTGGCGCAACAGCACCGGATTGTGTGTTACGCTGCGAAAATGCGGATTTTc                                                     >  2:367765/1‑300 (MQ=255)                                                                                                                                                                                                                                                             gggTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTACCGTCAGGTTTTTAACGGGCAACTGGCGCACCCGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCAGCCAAAGt                                       >  2:195668/3‑300 (MQ=255)                                                                                                                                                                                                                                                             gCGTTTTATGTGCGTTATCTGCAAATTCACGTGCGATGGTTTCGATATACCGGCGCTGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCTTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGt                                       <  2:360280/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGt                                       <  1:185163/300‑1 (MQ=255)                                                                                                                                                                                                                                                             gCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTAGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCAGTCGACTAACGTAAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTCCGCTGCGAAAATGCGGACTTTCAATGCCGCCAAAGt                                       >  2:196735/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           gTATCGGCAAATTCACGGGCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCAAAAGTAGGGGAAGGCCAc                          >  1:340273/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                             gCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGt       <  2:470597/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                             gCGATGGTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGt       <  1:577587/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  ggTTTCGATATACTGGCGCGGCACGCCAGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAAGTCTTTAACGGGCAATTAGCGCAACAGCACAGGATTGTGTTTTACGCTGCGAAAACGCGGATTTTCAATGCAGCAAACGTAGGGGAAGGCCACGCATGCAACAGTGTCATGTTGAcc  >  2:391854/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                      GGCTGGAATGATTGTAGAAAAACGAGGTGCTGTTCATTTGGCGCGGTGGTCGGTTCCAGTCGAGCGCAAAGGCCAGTGGCAACCAGCCGGTTTGTGGGCGCAGTTTTTCCTGGCCGACATAGTGTGCCCAGCCGCCACCGCTTTGCCCGACACAACCGCAGAAGATCAGCATATTGATCATCCCGCGGTAGTTCATGTCCATGTGATACCAGTGGTTAACACCTGCGCCGAGGATAATCATCGAGCGCCCATGCGTTTTATGGGCGGTATCGGCAAATTCACGGGCGATGGTTTCAATATACTGGCGCGGCACGCCGGTAATTTGCTCACCCCAGGCTGGGGTGTACGGTTTGATTTCAGCGTAATCTTTCGCACTGTTTTCATCTTCCAGCCCGCGATCGAGGCCGTAATTCGCCAGTACCAAATCATAAACGCTGACCACCGGACAGGTGTTGCCATCGACTAACGTCAGGTTTTTAACGGGCAATTGGCGCACCAGCACCGGATTGTGTTTTACGCTGCGAAAATGCGGATTTTCAATGCCGCCAAAGTAGGGGAAGGCCACGCCTGCAACAGCGTCATGTTGACC  >  NC_000913/1540742‑1541330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑