Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,676,087	A→G	S247P (TCT→CCT)	yphD ←	putative ABC transporter membrane subunit YphD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,676,087	0	A	G	97.8%	156.7 / NA	45	S247P (TCT→CCT)	yphD	putative ABC transporter membrane subunit YphD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (15/29);  minor base T (1/0);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 3.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCGCACCCGCGCCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACA  >  NC_000913/2675798‑2676380                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      tGCCGCACCCGCGCCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCNGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAgg                                                                                                                                                                                                                                                                                                <  1:523398/297‑1 (MQ=255) tGCCGCACCCGCGCCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGNGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAgg                                                                                                                                                                                                                                                                                                <  2:511325/297‑1 (MQ=255) tGCCGCACCCGCGCCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAgg                                                                                                                                                                                                                                                                                                <  1:91296/297‑1 (MQ=255)            cgcCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCcaacaa                                                                                                                                                                                                                                                                                  >  1:573989/1‑300 (MQ=255)                 ggggCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGc                                                                                                                                                                                                                                                                             <  2:606077/300‑1 (MQ=255)                   ggCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGcc                                                                                                                                                                                                                                                                            >  1:719131/1‑299 (MQ=255)                      gTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAANGTACCGAACAGGGAACCGCGACCGCCGGAAAGNGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTc                                                                                                                                                                                                                                                                        <  2:551250/300‑1 (MQ=255)                            gTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCGCCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAAAAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGt                                                                                                                                                                                                                                                                         >  2:641324/1‑293 (MQ=255)                                 gCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAACACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGACTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGNAACCGCGACCGCCGGAAAGTGCAGTACCGCCGACCACGACCGCGGCGATGACGTAAAACTCAAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGATCGCCGCCGCtaa                                                                                                                                                                                                                                                             >  2:233703/1‑300 (MQ=255)                                                           tCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGaa                                                                                                                                                                                                                                   <  2:68749/300‑1 (MQ=255)                                                                        cGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGCCCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGNGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCAGAAAGGGTAAAGATAAGAATGTGCa                                                                                                                                                                                                                             >  2:570819/1‑293 (MQ=255)                                                                            aGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGaa                                                                                                                                                                                                                    <  1:212516/298‑1 (MQ=255)                                                                                     accacGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCTATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGc                                                                                                                                                                                                           <  2:487791/298‑1 (MQ=255)                                                                                        acGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGcac                                                                                                                                                                                                      <  2:639090/300‑1 (MQ=255)                                                                                          gatgatGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCAGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACaa                                                                                                                                                                                                    >  2:696517/1‑300 (MQ=255)                                                                                           atgatgACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCTATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCTGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGGTACCAGGACCGAGGCGCGCCGCCAACAAACCGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAGTGCGCACCCGACGAACGTTGATTCCGCACAAc                                                                                                                                                                                                   <  2:258848/300‑1 (MQ=255)                                                                                           atgatgACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc                                                                                                                                                                                                   <  2:258938/300‑1 (MQ=255)                                                                                           atgatgACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc                                                                                                                                                                                                   <  1:286947/300‑1 (MQ=255)                                                                                           atgatgACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGGAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc                                                                                                                                                                                                   <  2:172509/300‑1 (MQ=255)                                                                                           atgatgACGCCGCGTACCACCTGCTGGAAAAAGGAGGTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAAc                                                                                                                                                                                                   <  2:171954/300‑1 (MQ=255)                                                                                             gatgaCGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTg                                                                                                                                                                                                 <  2:380352/300‑1 (MQ=255)                                                                                                          accaccTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCa                                                                                                                                                                                    <  1:212590/300‑1 (MQ=255)                                                                                                             accTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCAt                                                                                                                                                                                   <  1:495913/298‑1 (MQ=255)                                                                                                                tgctgGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCTATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCg                                                                                                                                                                              <  2:499358/300‑1 (MQ=255)                                                                                                                             aGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCAACAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTACAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGACGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCaaaaa                                                                                                                                                                 >  2:609132/1‑300 (MQ=255)                                                                                                                                       gAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCAGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCAGAAAGGGTAAAGATAAGAATGCGCACCCGACGAATGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAACGCCAACGGCAAAAACCGAGCGcc                                                                                                                                                        >  2:119359/1‑299 (MQ=255)                                                                                                                                            gcaCCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCCTTCCCGCCAACGGCAAAAACCGAGCGCCCGaa                                                                                                                                                    >  1:377762/1‑298 (MQ=255)                                                                                                                                            gcaCCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGaa                                                                                                                                                    >  1:377830/1‑298 (MQ=255)                                                                                                                                              aCCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc                                                                                                                                                 <  1:418607/299‑1 (MQ=255)                                                                                                                                              aCCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGAACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGc                                                                                                                                                 <  1:223573/299‑1 (MQ=255)                                                                                                                                                             aTTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCtgatg                                                                                                                                 <  2:717006/300‑1 (MQ=255)                                                                                                                                                                      aTCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGTCGATGACGTCANACTCCAGACCATTTGCGGCACCTGCGTTACCAGGACTGAGGCGCGCCGCCAACAAAATGCTGGGCACCGCCGCTAATAATCCTGAAATGGTAAAGAGAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAAc                                                                                                                        <  2:571667/300‑1 (MQ=255)                                                                                                                                                                                           gTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTNCCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCAt                                                                                                    >  1:149496/1‑299 (MQ=255)                                                                                                                                                                                           gTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCAGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGTGTTACCAGTACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCAGAAAGGGTAACGATAAGAATGCGCACCCGAAGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTNCCGACAACGGCAAAAAACGAGCGCACGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACCCAATCAt                                                                                                    >  1:149254/1‑299 (MQ=255)                                                                                                                                                                                                gAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATc                                                                                                <  2:454447/298‑1 (MQ=255)                                                                                                                                                                                                caccAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCa                                                                                               >  2:579582/4‑299 (MQ=255)                                                                                                                                                                                                  acatggAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGGCAAACTCCAGACCGTGTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATACCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAAAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCTTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATAAACACAAACAGCGCAAACAACACAATCACGATCAgcg                                                                                            <  2:481009/296‑1 (MQ=255)                                                                                                                                                                                                    aGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAgcgc                                                                                           <  1:571162/299‑1 (MQ=255)                                                                                                                                                                                                                           tGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTg                                                                   <  1:405726/300‑1 (MQ=255)                                                                                                                                                                                                                                                 gtgtcgATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGTTAAAGATAAGAATGCGCACCCTACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCACCCGAAGGCGGTTTTGCGGCTGATGAACACAAACATCGCAAACAACACACCCATGATCGTCACGAGGATGTGAACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCAc                                              <  2:129569/295‑1 (MQ=255)                                                                                                                                                                                                                                                           cGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCCCCGCCGCTAATAATCCAGAAAGGGTAAAGGTAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTAACGCCAACGGCAAAAACCGAGCGCACGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAAAACAATCATGATCAGCGCGGATAACGGCACACCGAGGAATTGTCCGCCCAGCCAGTCCAGCAccgaggtttcat                                   >  2:86347/1‑290 (MQ=255)                                                                                                                                                                                                                                                                               gCGGCACCTGCGTTACCAGGACCGAGGCGCGCCGCCAACAGAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCTGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCACGGAGACCTGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCg               <  2:719131/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ttACCAGGACCTAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAAc   <  1:119359/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            tACCAGGACCGAGGCGCGCCGCCAACACAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCACGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACAAAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTcc                                                                >  2:184678/1‑238 (MQ=255)                                                                                                                                                                                                                                                                                            tACCAGGACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACa  >  1:651400/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      TGCCGCACCCGCGCCAGGGGCGTTAAGGTTTAGCGTTTCGCTTTACTGCTTCGCTGGGTCAGCAAGATATTCGCCAGCACCGCCACCACGATGATGACGCCGCGTACCACCTGCTGGAAAAAGGAGTTAATACCGAGCAGCACCAGACCGTTACCGATTAGCGTAATCACCAGCACACCAAGCAATGTACCGAACAGGGAACCGCGACCGCCGGAAAGTGCCGTACCGCCGACCACGACCGCGGCGATGACGTCAAACTCCAGACCGTTTGCGGCACCTGCGTTACCAGAACCGAGGCGCGCCGCCAACAAAATGCCGGTCACCGCCGCTAATAATCCCGAAAGGGTAAAGATAAGAATGCGCACCCGACGAACGTTGATGCCGCACAACTGCGCCGCCGTAGCATTACCGCCAACGGCAAAAACCGAGCGCCCGAAGGCGGTTTTGCGGCTGATGAACACAAACAGCGCAAACAACACAATCATGATCAGCGCGGATACCGGCACACCGAGAAATTGTCCGCCCAGCCAGTCCAGCACCTCGTTTTCATCAATCGGCACTGGCAGTGCGTTCGTCATAAACA  >  NC_000913/2675798‑2676380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑