Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,395,367	G→A	R186C (CGT→TGT)	ynaI ←	small conductance mechanosensitive channel YnaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,395,367	0	G	A	100.0%	334.3 / NA	97	R186C (CGT→TGT)	ynaI	small conductance mechanosensitive channel YnaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (51/46);  total (51/46)

GAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACC  >  NC_000913/1395072‑1395662                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         gagTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTNCAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATc                                                                                                                                                                                                                                                                                                      <  1:515344/300‑1 (MQ=255) gagTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATc                                                                                                                                                                                                                                                                                                      <  1:670892/300‑1 (MQ=255)     cAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGAAGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTAGATATTTCTGTCCGGTGAACAGATcc                                                                                                                                                                                                                                                                                                     >  2:461696/1‑297 (MQ=255)         aaaCTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGCCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAAGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGcc                                                                                                                                                                                                                                                                                              >  2:169807/1‑300 (MQ=255)              ggTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATAc                                                                                                                                                                                                                                                                                         <  1:685229/300‑1 (MQ=255)                       aaaCCAGTAAGGTTTGTCGCTGTTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGa                                                                                                                                                                                                                                                                                 <  2:105252/299‑1 (MQ=255)                       aaaCCAGGAAGGTTTGTCGTTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCTGTctcttatacacatctcgc                                                                                                                                                                                                                                                                                <  1:304529/300‑18 (MQ=255)                          ccAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGt                                                                                                                                                                                                                                                                             >  1:163660/1‑300 (MQ=255)                                aGGTTTGTCGCTGGTCGATGTCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAAt                                                                                                                                                                                                                                                                       <  1:428812/300‑1 (MQ=255)                                aGGTTTGTCGCGGTTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATTCGGCGGTTGTTCATTCTTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAAt                                                                                                                                                                                                                                                                       <  2:470302/300‑1 (MQ=255)                                 ggTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATGACGCACACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTCGGTAATCCGCCAGCCAATTTCCGCTACTGTACATTCGATATTTCTGTCCGGTGAACAGCTCCAGTCGCCAATACTGAAAGGACGGTCGCAATa                                                                                                                                                                                                                                                                      >  2:196307/1‑300 (MQ=255)                                 ggTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTCAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTTGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTATGTACGGTGAAAAGATCCAGTCGCAAATACTGAAAGGACGGCCGAAATa                                                                                                                                                                                                                                                                      >  2:198331/1‑300 (MQ=255)                                 ggTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCAAATACTGAAAGGACGGTCGAAATa                                                                                                                                                                                                                                                                      >  2:196401/1‑300 (MQ=255)                                 ggTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTAATGTACTTTCGCTATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATa                                                                                                                                                                                                                                                                      >  2:52425/1‑300 (MQ=255)                                 ggTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTAGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTCCAATGGACGATTATCAAAGATCGTAATTTTGATAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCATTCGCACATACTGAAAGGACGGTCGAAATa                                                                                                                                                                                                                                                                      >  2:197235/1‑300 (MQ=255)                                     tGTCGCTGTTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGc                                                                                                                                                                                                                                                                  <  1:340932/300‑1 (MQ=255)                                                        ggTGATTTTTCAGCATCTCACGTACAGCTTCGACAAGAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAaagaag                                                                                                                                                                                                                                                <  1:257507/299‑1 (MQ=255)                                                           gATTTTTCAGCATCTCACTTACAGCTTCGACAATAACGCCCACTTTTTCCGCATCCTCATAACGTAAACCAATGGTCGTGGCAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGc                                                                                                                                                                                                                                                                  <  2:440911/278‑1 (MQ=255)                                                                      aTCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGNGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCt                                                                                                                                                                                                                                 >  1:507947/1‑300 (MQ=255)                                                                       tctcACGTACAGCTTTGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGTTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGTAAAAGAAGTTACTCAGAATATCtt                                                                                                                                                                                                                                <  2:84245/300‑1 (MQ=255)                                                                        ctcACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCttt                                                                                                                                                                                                                               >  1:214445/1‑300 (MQ=255)                                                                           aCGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTAcc                                                                                                                                                                                                                            >  2:7411/1‑300 (MQ=255)                                                                            cGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATCCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCCGTCGCCAATACTGAAAGGCCGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATATTTAcc                                                                                                                                                                                                                            >  2:362831/1‑299 (MQ=255)                                                                                     tCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGTTCATTCGTCCTTGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAAGGTGGGTAATTTGTAAGCAAATTTCTGCGACGGGACCGGCGATATTTCTGTTTGGTGAACAGATCCAGGCGCCAATACGGAAAGGACGGTTGGGGGAGAGAATAATTCCGGAAaagaaggtggtcgggggggcgtggctggcaggggag                                                                                                                                                                                                                  <  1:651989/300‑31 (MQ=255)                                                                                       gACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGAc                                                                                                                                                                                                                <  1:442726/300‑1 (MQ=255)                                                                                                gCCCATTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGGAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGac                                                                                                                                                                                                        <  1:586994/299‑1 (MQ=255)                                                                                                    aCTTTTTCCGCATCCTCATAACGTAAACCAATGTTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGacca                                                                                                                                                                                                      <  2:424045/297‑1 (MQ=255)                                                                                                     cTTTTGCCGCATCCTCATAACGTAACCCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCCGAATATCCTTCCCGGCCATACCGACCGCCAGAccaccac                                                                                                                                                                                                  >  2:396810/1‑299 (MQ=255)                                                                                                       tttGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATa                                                                                                                                                                                                <  1:485665/300‑1 (MQ=255)                                                                                                             gCATCCTCATAACGTAAACCAATGGTCGTGGTAATTCGGCGGTTGGTCATTCGTCCGGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATAccac‑ca                                                                                                                                                                                         <  2:622520/300‑1 (MQ=255)                                                                                                               aTCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑Caa                                                                                                                                                                                        <  2:438468/299‑1 (MQ=255)                                                                                                                  cTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAAt                                                                                                                                                                                                                                                             >  2:174621/1‑228 (MQ=255)                                                                                                                  cTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCAATACCGACAGCCAGACCACCAATACCAC‑CAAAGGt                                                                                                                                                                                    >  1:299113/1‑300 (MQ=255)                                                                                                                  cTCAGAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGGAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAAt                                                                                                                                                                                                                                                             <  1:174621/228‑1 (MQ=255)                                                                                                                   tCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACTGGCCATACCGACAGCCAGACCACCAATACCAC‑AAAAGGt                                                                                                                                                                                    >  2:57410/1‑299 (MQ=255)                                                                                                                   tCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACATACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGAACACCACTACCAC‑CAAAGGt                                                                                                                                                                                    >  2:387552/1‑299 (MQ=255)                                                                                                                   tCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGATACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATaccaaca                                                                                                                                                                                          >  2:57311/1‑291 (MQ=255)                                                                                                                   tCATAACGTAAACCAATGGTCGTGGGAATGCGGCGGGTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCtt                                                                                                                                                                                                                                <  2:331270/256‑1 (MQ=255)                                                                                                                             aaCCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCa                                                                                                                                                                          >  1:721802/1‑299 (MQ=255)                                                                                                                                 aaggttCGTGGTAATGCGGCGGTTGGTAGTTCGTCCTGGTTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGTTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  2:108777/294‑1 (MQ=255)                                                                                                                                 aaTGTTCGTGGTAAGTCGGCGGTTGTTCATTCGTCTTGGTTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATAGTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  2:69436/299‑1 (MQ=255)                                                                                                                                 aaTGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  1:79351/299‑1 (MQ=255)                                                                                                                                 aaTGGTCGTGGTAAGGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCGGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  1:345452/299‑1 (MQ=255)                                                                                                                                 aaTGGTCGTGGGAATGTGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  1:344208/299‑1 (MQ=255)                                                                                                                                     gTCGTGGTAATTCGGCGGTTGTTCATTCGTCCTGGTTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTc                                                                                                                                                                 <  2:684686/300‑1 (MQ=255)                                                                                                                                      tCGTGGTAATGCGGCGGTTGTTCATTCGTCCGGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTc                                                                                                                                                                 <  1:656704/299‑1 (MQ=255)                                                                                                                                      tCGTGGTAATGCGGCGGTTGGTCATTCGTCTTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTATCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATACCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGaaa                                                                                                                                                                      <  1:345445/294‑1 (MQ=255)                                                                                                                                         tGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGc                                                                                                                                                             >  1:87972/1‑300 (MQ=255)                                                                                                                                         tGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTCCCGGCCATACCGACAGCCAGACCACCAATACCAC‑CCACGGTCAGCAAGCCAGAAAGGCTCATGc                                                                                                                                                             >  2:349477/1‑300 (MQ=255)                                                                                                                                         tGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGc                                                                                                                                                             >  1:342855/1‑300 (MQ=255)                                                                                                                                         tGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCAAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAACGGCTCAttc                                                                                                                                                             >  2:353093/1‑298 (MQ=255)                                                                                                                                         tGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCAAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTAACGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCACGCCAGAAAGGCTCATGc                                                                                                                                                             >  1:262575/1‑300 (MQ=255)                                                                                                                                                                 gTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTAAGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCCGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGaa                                                                                                                                     >  2:647134/1‑300 (MQ=255)                                                                                                                                                                  tCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCTTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGaa                                                                                                                                     <  2:376675/299‑1 (MQ=255)                                                                                                                                                                      gggTTTTCTACGCGAATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGTTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAATGCTCATGCCGAAATGTTCGCCATAAAGCAg                                                                                                                                       <  2:329041/293‑1 (MQ=255)                                                                                                                                                                       ggTTTTCGACGCTGAGCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTGATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGca                                                                                                                               <  2:67461/300‑1 (MQ=255)                                                                                                                                                                         ttttCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGcacc                                                                                                                             <  2:447219/300‑1 (MQ=255)                                                                                                                                                                          tttCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGAAAGCCAGACCACCAATACCAC‑CAAAGGTAAGCAAGCCAGAAAGGCTCATGCAGAAATGTTCGCAATAAAGCAGAACAAGcacca                                                                                                                            >  2:321157/1‑300 (MQ=255)                                                                                                                                                                               aCGCTGATCGACGAAAACAGCGAGTTTGGTACGTACAATGGACGATGATCAAAGGTCTTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACaat                                                                                                                        <  2:93271/299‑1 (MQ=255)                                                                                                                                                                                 gCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACaataa                                                                                                                      <  1:13022/299‑1 (MQ=255)                                                                                                                                                                                 cctgcgagatgtgtataagagacagggTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACaataat                                                                                                                     >  1:550457/26‑300 (MQ=255)                                                                                                                                                                                    gATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACaagaaggg                                                                                                                   <  1:664328/299‑7 (MQ=255)                                                                                                                                                                                    gATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAA‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAAAATGATGGt                                                                                                                  >  1:271240/1‑300 (MQ=255)                                                                                                                                                                                              aaCAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTtgat                                                                                                         >  2:80097/1‑299 (MQ=255)                                                                                                                                                                                                      gTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGa                                                                                                >  1:708907/1‑300 (MQ=255)                                                                                                                                                                                                          ggTACGTACACTGGACGATTATCAAAGTTCGTAATTTTGGTAATTCGCCAGCCAGTTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACAt                                                                                             <  1:383752/299‑1 (MQ=255)                                                                                                                                                                                                          ggTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACAt                                                                                             <  1:384024/299‑1 (MQ=255)                                                                                                                                                                                                          ggTACGTACAATGGACGATTATCAAAGGTCGTAATTCTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCGTAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATa                                                                                            >  2:494700/1‑300 (MQ=255)                                                                                                                                                                                                             acttacaggggACGATTACCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCANAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATc                                                                                         <  2:214190/291‑1 (MQ=255)                                                                                                                                                                                                             acgtacAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTNTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATc                                                                                         <  1:528447/300‑1 (MQ=255)                                                                                                                                                                                                             acgtacAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATc                                                                                         >  1:88455/1‑300 (MQ=255)                                                                                                                                                                                                               gtacAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATGCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAAAATAATGGTGATTTTGATGATACGCGAAATAATcc                                                                                        >  2:493935/1‑299 (MQ=255)                                                                                                                                                                                                                  cAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGACATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCcgc                                                                                      >  2:291397/1‑298 (MQ=255)                                                                                                                                                                                                                    gtGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCGATGCTGAAAGGAAGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTAAGAATATCTTTACCGGCCATACCGACAGCAAGACCACCAATCCCACACAAAG‑TCCGCCAGCCAGAAAGGCTCATGCCGAAATGTTCGGCATAAAGCAGAACACGCACCACAATAATGGTGATTTTTATGATACGCGACATAAGCCGCGCAc                                                                                  >  2:704379/2‑300 (MQ=255)                                                                                                                                                                                                                     tGGACGAGTATCAAAGGTCGTAATTTTGTTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATcc                                                                                        <  2:365381/293‑1 (MQ=255)                                                                                                                                                                                                                          gATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGa                                                                            <  1:461696/300‑1 (MQ=255)                                                                                                                                                                                                                            ttATCAAAGGTCTTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTGACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGata                                                                          <  2:473474/300‑1 (MQ=255)                                                                                                                                                                                                                             tagcAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGAcctgtctcttatacacatctcgc                                                                         <  1:81197/297‑23 (MQ=255)                                                                                                                                                                                                                             gaTCAAAGGTTGTAATTTTGGGAATTCGCCAGCCAATTTCCGCGACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAACCAGACCACCAATACCAC‑CAAAGGTCAGCAGGCCAGAAAGACTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGTTGATTTTGATGATACGCGAcctgtctcttatacacatctcgc                                                                         <  1:81382/299‑23 (MQ=255)                                                                                                                                                                                                                              ataaaagttcttAATTTTGGTACTTCGCAAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTTAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGANAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATc                                                                        <  2:516886/289‑1 (MQ=255)                                                                                                                                                                                                                                                                aTTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGAACTTTTTTAATCTGCTGTTTTTCAAACTGa                                      >  1:297540/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    ccGCTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTaa                                  >  1:703740/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATca                               >  1:372198/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCAACAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTAAAACTGATTAATca                               >  1:371845/1‑300 (MQ=255)                                                                                                                                                                                                                                                                       cTACTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACANTAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATca                               >  2:566400/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         aCTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATcagc                             >  1:356751/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         aCTGTACCTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCAGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAA‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGCAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATcagc                             >  2:292796/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                cTTCGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCAAAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATa                      >  2:439952/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   cGATATTTCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCt                   >  2:436921/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                        tttCTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATNATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATc              >  1:522084/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                           cTGTCCGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTc           >  1:117942/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               ccGGTGAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATANAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGaa       >  2:225073/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 ggTGAACAGATCCAGTCGCCAATACGGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAAt      <  2:163660/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                    gAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAAcc  >  2:192303/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    gAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAAcc  >  1:255763/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                    gAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTAAGAATa     >  2:117506/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                                    gAACAGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGATCATGCCGAAATGTTCGACATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGAACTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAAcc  >  2:412805/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                         GAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGTTTGTCGCTGGTCGATGGCCGGGTGATTTTTCAGCATCTCACGTACAGCTTCGACAATAACGCCCACTTTTGCCGCATCCTCATAACGTAAACCAATGGTCGTGGTAATGCGGCGGTTGGTCATTCGTCCTGGGTTTTCTACGCTGATCGACGAAAACAGCGAGTTCGGTACGTACAATGGACGATTATCAAAGGTCGTAATTTTGGTAATTCGCCAGCCAATTTCCGCTACTGTACCTTCGATATTTCTGTCCGGTGAACGGATCCAGTCGCCAATACTGAAAGGACGGTCGAAATAGAGCATAATCCCGGAAAAGAAGTTACTCAGAATATCTTTACCGGCCATACCGACAGCCAGACCACCAATACCAC‑CAAAGGTCAGCAAGCCAGAAAGGCTCATGCCGAAATGTTCGCCATAAAGCAGAACAAGCACCACAATAATGGTGATTTTGATGATACGCGACATAATCCGCGCACTGGTGATATCGCGACCTTTTTTAATCTGCTGTTTTTCAAACTGATTAATCAGCAGAAATAGCTTAATCGTCAGAATAACC  >  NC_000913/1395072‑1395662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑