Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	780,624	C→T	noncoding (71/76 nt)	lysT →	tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	780,624	0	C	T	94.9%	258.1 / ‑7.0	80	noncoding (71/76 nt)	lysT	tRNA‑Lys
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base T (36/38);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCC  >  NC_000913/780328‑780914                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    tCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACtcac                                                                                                                                                                                                                                                                                                  >  1:9602/1‑300 (MQ=255)    gTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACccaccag                                                                                                                                                                                                                                                                                               <  1:165412/300‑2 (MQ=255)           cccTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTaa                                                                                                                                                                                                                                                                                        >  1:648188/1‑300 (MQ=255)           cccTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGATaa                                                                                                                                                                                                                                                                                        >  2:79692/1‑300 (MQ=255)             cTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGCCTCACCAATCGCTac                                                                                                                                                                                                                                                                                        >  2:193202/1‑297 (MQ=255)              tGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAATTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGt                                                                                                                                                                                                                                                                                     <  1:192341/300‑1 (MQ=255)              tGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAgg                                                                                                                                                                                                                                                                                      <  2:648966/299‑1 (MQ=255)               ggTACTGAGGGCACTAAACAGGCACAAAAACGTCGGAACACGATGTAATGCATAACACACTACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTg                                                                                                                                                                                                                                                                                    <  2:105844/300‑1 (MQ=255)                gTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGAATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGCAATTTTTGTTGCGCTCAAGTCTGAAATCAGTCATATATGCCGCCGTTGCCCAGGGATATCAAACAAACCGAAAGCAACGCAAAAGTGGGTCGTTAGTTCATTTGGTAGAGTAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCCCGCATCACCAATCGCTaatgt                                                                                                                                                                                                                                                                                     >  1:295738/1‑295 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCATGACTCACCAATCGCCAAgttgaaagt                                                                                                                                                                                                                                                                               >  2:325681/1‑292 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGc                                                                                                                                                                                                                                                                               <  1:79692/300‑1 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAACCGCTAAGGGGGaa                                                                                                                                                                                                                                                                                 >  2:325727/1‑298 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAAAGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGt                                                                                                                                                                                                                                                                                     >  2:325693/1‑294 (MQ=255)                    tGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAAGGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGc                                                                                                                                                                                                                                                                               <  2:726457/300‑1 (MQ=255)                        ggCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAATCACCAATCGCTAAGGTGGAAGAGgta                                                                                                                                                                                                                                                                           >  2:372577/1‑300 (MQ=255)                         gcgcTAAACATGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGTTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGc                                                                                                                                                                                                                                                                               <  2:297650/295‑1 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGACAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAATCACCAATCGCTCAGGTGGAAGCggca                                                                                                                                                                                                                                                                           >  2:367466/1‑297 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATc                                                                                                                                                                                                                                                                                             >  1:665250/1‑281 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGc                                                                                                                                                                                                                                                                               >  1:297650/1‑295 (MQ=255)                         gcgcTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGAACAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGgta                                                                                                                                                                                                                                                                           >  2:45761/1‑299 (MQ=255)                                    gCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGaa                                                                                                                                                                                                                                                               >  1:473872/1‑300 (MQ=255)                                    gCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTAAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAAc                                                                                                                                                                                                                                                                    >  2:186222/1‑295 (MQ=255)                                           aaCGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGTTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAAc                                                                                                                                                                                                                                                        <  2:472363/300‑1 (MQ=255)                                              gTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGt                                                                                                                                                                                                                                                      <  1:559969/299‑1 (MQ=255)                                                  gAACTCGATGTAATGCATGACACACGACCAGAAGTCGTATTATTTCTGTTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATANGCCGCCGTTGCTACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGTTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGt                                                                                                                                                                                                                                                      <  2:235613/295‑1 (MQ=255)                                                         aTGTAGTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGTATGAGCAAc                                                                                                                                                                                                                                          >  2:440800/1‑300 (MQ=255)                                                         aTGTAATGCATAACACACGAACAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCCAAGGTGGAAGCGGTAGTAAAACGTGAAGGATACCGTTGcccgagaaac                                                                                                                                                                                                                                          >  2:175211/1‑291 (MQ=255)                                                           gTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAAt                                                                                                                                                                                                                                                                                              >  1:73379/1‑246 (MQ=255)                                                           gTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTCAAACGTGGAGGATAACGTTGCATGAGCAAc                                                                                                                                                                                                                                          >  1:111210/1‑298 (MQ=255)                                                           gTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAAc                                                                                                                                                                                                                                          >  1:192447/1‑298 (MQ=255)                                                           gTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGtt                                                                                                                                                                                                                                                     >  2:125673/1‑287 (MQ=255)                                                             aaTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCaa                                                                                                                                                                                                                                                                                               >  2:452857/1‑243 (MQ=255)                                                              aTGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCAACAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGTAACGGcac                                                                                                                                                                                                                                     >  2:241887/1‑298 (MQ=255)                                                                gCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGa                                                                                                                                                                                                                                   >  2:459386/1‑300 (MQ=255)                                                                       acacGACCAGAAGTCGCATTATTTCTGTTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGTGCga                                                                                                                                                                                                                            <  2:416599/300‑1 (MQ=255)                                                                          cGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTCATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGAc                                                                                                                                                                                                                         >  1:375345/1‑300 (MQ=255)                                                                                gAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCACGAAGGGCGAGACGAAGTc                                                                                                                                                                                                                   >  1:448708/1‑300 (MQ=255)                                                                                        actattTCTGGTAGGGTCGTGCGAATCATAAGCAGTTGATTG‑ATCTACATCGAAATTTTTTTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCTAGACGAAGTCGAGTCAt                                                                                                                                                                                                            <  2:474497/297‑1 (MQ=255)                                                                                         ttattTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGGGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATcc                                                                                                                                                                                                          >  2:212159/1‑300 (MQ=255)                                                                                           attTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGAAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCTAGACGAAGTCGAGCCATCCt                                                                                                                                                                                                         >  2:283407/1‑299 (MQ=255)                                                                                              tCTGTTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  1:173546/300‑1 (MQ=255)                                                                                              tCTGGTCGTGTTGGGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  2:100716/300‑1 (MQ=255)                                                                                              tCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTNATATATGCCGCCGTTGCCACTGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  1:511534/300‑1 (MQ=255)                                                                                              tCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     <  1:186016/300‑1 (MQ=255)                                                                                                 ggTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGAc                                                                                                                                                                                                  <  1:389074/300‑1 (MQ=255)                                                                                                 ggTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑AGCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGTTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCTAGACGAAGTCGAGTCACCCTGCACGAc                                                                                                                                                                                                  <  1:389059/300‑1 (MQ=255)                                                                                                     gtgtcgtgCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAGACGTGGAGGATAACGTTGCATGAGCAACGGCACGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACccac                                                                                                                                                                                              >  2:83072/1‑300 (MQ=255)                                                                                                           tgCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCACGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACtca                                                                                                                                                                                        >  2:289988/1‑298 (MQ=255)                                                                                                           tacaaaccATAAACAGTTGAGTGGAT‑TACATAGAAAGTTTTGTGGCGATCAAGTCGGAAAGCAGGAATACATGCCGCCGGTGACACGGGATATAAAACAAACCTAAAGTAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAGTTGCGAAGTTGGAGGCGGGAGTGAAGCGTGGAGGATAACGTTGCATGAGCAACGGCCCGTAGGGCGAG‑CGAAG‑CGAGTCATCCTGCACGACCCACCACTaatg                                                                                                                                                                                      <  2:686518/293‑3 (MQ=12)                                                                                                            gCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATAGGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCGAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAc                                                                                                                                                                                       <  2:343127/300‑1 (MQ=255)                                                                                                               aaTCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGTTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACAt                                                                                                                                                                                     <  2:123671/299‑1 (MQ=255)                                                                                                                       gCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCACGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCAATAACa                                                                                                                                                                                      >  2:428170/1‑290 (MQ=255)                                                                                                                       gCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGNTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAAcgacggtgggtt                                                                                                                                                                            >  2:517895/1‑289 (MQ=255)                                                                                                                          gTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTgtag                                                                                                                                                                         <  1:462505/300‑1 (MQ=255)                                                                                                                          gTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCATGCACGACCCACCAATCTCCACGTTGAAGGCAGTCGTCAAACGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCgagcgaa                                                                                                                                                                                                                       >  2:585457/1‑250 (MQ=21)                                                                                                                                 g‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAcctgtctcttatacacatctcgcag                                                                                                                                                                             <  1:654798/289‑25 (MQ=255)                                                                                                                                 g‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                                                                                                                                                     >  2:654798/1‑265 (MQ=255)                                                                                                                                   aTCTACATCGAAATTTTTGTTGCGCTCAAGGCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAANCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGc                                                                                                                                                                 <  1:528583/300‑1 (MQ=255)                                                                                                                                      tACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCgt                                                                                                                                                               <  1:186222/299‑1 (MQ=255)                                                                                                                                          tCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTAt                                                                                                                                                          <  1:4230/300‑1 (MQ=255)                                                                                                                                               aTTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCAGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGt                                                                                                                                                     <  1:202366/300‑1 (MQ=255)                                                                                                                                                               aGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGCCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCtaagat                                                                                                                                        >  2:720308/1‑292 (MQ=255)                                                                                                                                                                gtcggAAATCAGTAATATATGCCTCCGCTGCCACGGGACATCAAACAAACCTAAAGCAACGAAAAAGTGGGTCGTTAGTTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGTTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATGACGTTGCATTAGCAACGGCCCGAAGGGCTAGACGAAGCCGAGTCATCCTGCACGACCTACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATTGGGTGATTAGCTCAGCTGGga                                                                                                                                    <  2:421484/296‑1 (MQ=37)                                                                                                                                                                 tCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGgag                                                                                                                                   <  1:466623/300‑1 (MQ=255)                                                                                                                                                                                 tatGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACaa                                                                                                                   <  1:459386/300‑1 (MQ=255)                                                                                                                                                                                       gccgTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGANTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAaggag                                                                                                               <  1:159019/298‑1 (MQ=255)                                                                                                                                                                                                        tCAAACAAACCGAAAGCAACGAAAAGGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGGGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATcc                                                                                            <  1:42495/300‑1 (MQ=255)                                                                                                                                                                                                                   gaaaacagcGAAAAAGTGGTTCGTTAACTCAGTTGGGAGAACAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGGAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCTTAGTATCGGGTGATTAGCTCAGTTGGGAGAGCACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                                                                                 <  2:19568/292‑1 (MQ=37)                                                                                                                                                                                                                   gAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcaccc                                                                                 <  2:19357/300‑1 (MQ=255)                                                                                                                                                                                                                   gAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATcac                                                                                   <  1:657143/298‑1 (MQ=255)                                                                                                                                                                                                                        aaaCGAAAAAGTGGGTGGTGAGTTCAGGGAGGAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACGCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGTAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccac                                                                            <  2:243492/299‑1 (MQ=37)                                                                                                                                                                                                                         aaCGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACGTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccac                                                                            <  2:192447/299‑1 (MQ=255)                                                                                                                                                                                                                                        agTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTc                                                             <  2:457952/298‑1 (MQ=255)                                                                                                                                                                                                                                          ttAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTcct                                                           >  1:457952/1‑297 (MQ=255)                                                                                                                                                                                                                                            aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTCGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTACATTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTAGTTAGATCAGtt                                                         >  1:471305/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                      atgctCGAATCCTGCACGACGAACCAATCGATAAGGGGGAAGAAGTAGTAAAACAGGAAGGATAACGTTACATGAGCAACAGCCCGAAAGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACGAACATAGTTAGTTGTAGTATCCATCGTAGTATCGGTTGATTAGCTCGGCGGGGAGGGCACCTCTCTTACACTTAGGTGGTCGGCGGTTCGAGCCCGGCATCACCCACCAC‑CTGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAAt              <  2:295738/295‑1 (MQ=21)                                                                                                                                                                                                                                                                                         ttCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCcaccact                                                                           >  1:125827/1‑235 (MQ=255)                                                                                                                                                                                                                                                                                         ttCGAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCt           >  1:126679/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                            gAATCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCAc       <  1:103322/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                               tCCTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAc    <  1:372577/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 cTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTACCATAGTTCGTTGTAGTATCCAGCGTCGTATCGTGTGATTCGTTTAGCTCGGTGAGCACCTCCCTTACACGAAGGGGGTCGACGGTTCGATCTAGTCATCCCCCACCAC‑CGGGTCGTTAGTTCAGTTGGTAGAGCAGTTGGCTTTTAATTAATTGGTCGCAGGTTCGAATCCTGCACGAccc  >  1:385827/1‑300 (MQ=37)                                                                                                                                                                                                                                                                                                 cTGCACGACTCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGTGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAA‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGAccc  >  1:163486/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                                    TCAGTAAATACCCTGGTACTGATGGCGCTAAACAGGCACAAAAACGTCTGAACGCGATGTAATGCATAACACACGACCAGAAGTCGCATTATTTCTGGTCGTGTCGTGCGAATCATAAGCAGTTGAGTG‑ATCTACATCGAAATTTTTGTTGCGCTCAAGTCTGAAATCAGTAATATATGCCGCCGTTGCCACGGGATATCAAACAAACCGAAAGCAACGAAAAAGTGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATCGCTAAGGTGGAAGCGGTAGTAAAACGTGAAGGATAACGTTGCATGAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCACTAACATAGTTAGTTGTAGTATCCAGCGTAGTATCGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAC‑CGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCC  >  NC_000913/780328‑780914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑