breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 40,387 C→T intergenic (‑1/+30) caiA ← / ← caiT crotonobetainyl‑CoA reductase/L‑carnitine:gamma‑butyrobetaine antiporter
RA 43,636 G→A G150D (GGC→GAC)  fixB → putative electron transfer flavoprotein FixB
RA 109,960 G→A R561H (CGT→CAT)  secA → protein translocation ATPase
JC JC 169,339 IS2 (+) +5 bp coding (1856‑1860/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 313,543 G→A intergenic (‑301/‑1748) ykgR ← / → insE1 putative membrane protein YkgR/IS3 element protein InsE
RA 339,935 C→T A56V (GCC→GTC)  yahH → putative uncharacterized protein YahH
RA 339,941 C→T T58I (ACT→ATT)  yahH → putative uncharacterized protein YahH
RA 339,958 G→A D64N (GAC→AAC)  yahH → putative uncharacterized protein YahH
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
MC JC 682,992 Δ4,859 bp IS5‑mediated [hscC]gltI [hscC], rihA, gltL, gltK, gltJ, sroC, gltI
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
JC JC 789,812 IS2 (–) +5 bp coding (164‑168/1149 nt) galK ← galactokinase
RA 791,688 G→A S123F (TCC→TTC)  galE ← UDP‑glucose 4‑epimerase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,100,070 G→A C144Y (TGT→TAT)  ycdY → chaperone protein YcdY
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,123,556 C→T E167K (GAA→AAA)  grxB ← reduced glutaredoxin 2
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,310,770 A→G H32H (CAT→CAC yciI ← protein YciI
RA 1,331,947 C→T G300G (GGC→GGT topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,412,897 G→A L113L (CTC→CTT intR ← putative integrase
RA 1,428,087 C→T E214K (GAG→AAG)  insH5 ← IS5 family transposase and trans‑activator
RA 1,610,076 T→G D202A (GAT→GCT)  uxaB ← tagaturonate reductase
RA 1,610,440 C→T E81K (GAA→AAA)  uxaB ← tagaturonate reductase
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,671,710 C→G intergenic (+26/‑67) asr → / → ydgU periplasmic chaperone Asr/uncharacterized protein YdgU
RA 1,721,136 C→T L38F (CTT→TTT)  ydhI → DUF1656 domain‑containing protein YdhI
RA 1,738,377 C→T H178H (CAC→CAT purR → DNA‑binding transcriptional repressor PurR
RA 1,776,715 A→T S377C (AGT→TGT)  ydiF → putative acetate‑CoA transferase YdiF
RA 1,856,968 T→A I268F (ATT→TTT)  ydjI ← L‑glycero‑L‑galacto‑octuluronate‑1‑phosphate aldolase
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,406 IS2 (–) +5 bp coding (26‑30/210 nt) cspC ← transcription antiterminator and regulator of mRNA stability CspC
RA 1,975,224 G→A A34V (GCG→GTG)  cheA ← chemotaxis protein CheA
RA 1,987,499 C→A intergenic (+71/+8) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
JC JC 2,239,800 IS30 (+) +2 bp coding (548‑549/999 nt) mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
JC JC 2,471,079 +A :: IS3 (+) +5 bp coding (435‑439/441 nt) yfdK ← putative tail fiber assembly protein YfdK
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,536,059 C→A H76N (CAC→AAC)  crr → Enzyme IIA(Glc)
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,682,766 IS1 (+) +9 bp intergenic (‑21/‑89) yphG ← / → yphH DUF5107 domain‑containing protein YphG/putative DNA‑binding transcriptional regulator, NAGC‑like
JC JC 2,698,402 IS30 (+) +2 bp coding (148‑149/636 nt) pgpC ← phosphatidylglycerophosphatase C
RA 2,700,747 G→A L84L (CTC→CTT acpS ← holo‑[acyl‑carrier‑protein] synthase
RA 2,748,396 G→A A145A (GCG→GCA ypjD → cytochrome c assembly family protein
RA 2,756,976 G→A R273Q (CGA→CAA)  intA → integrase IntA
RA 2,780,060 G→A H889H (CAC→CAT ypjA ← adhesin‑like autotransporter YpjA
RA 2,792,209 G→A V313M (GTG→ATG)  gabD → succinate‑semialdehyde dehydrogenase (NADP(+)) GabD
RA 2,800,435 G→A G97D (GGT→GAT)  ygaM → DUF883 domain‑containing protein YgaM
RA 2,810,925 G→A G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator MprA
RA 2,817,850 G→A noncoding (11/77 nt) argQ ← tRNA‑Arg
RA 2,825,328 G→A S83S (TCC→TCT mltB ← membrane‑bound lytic murein transglycosylase B
RA 2,841,238 G→A M83I (ATG→ATA ascB → 6‑phospho‑beta‑glucosidase AscB
RA 2,863,773 C→T L61F (CTT→TTT)  ygbL → putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
RA 2,878,077 C→T intergenic (+459/+492) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,889,365 C→T K245K (AAG→AAA cysI ← sulfite reductase, hemoprotein subunit
RA 2,905,825 C→T Q39* (CAA→TAA)  ygcG → TPM domain‑containing protein YgcG
JC JC 2,913,392 IS2 (–) +5 bp coding (256‑260/2235 nt) relA ← GDP/GTP pyrophosphokinase
RA 2,926,292 C→T intergenic (+96/‑16) queF → / → ppnN 7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
RA 3,088,010 2 bp→TT intergenic (+150/‑273) metK → / → galP methionine adenosyltransferase/galactose:H(+) symporter
RA 3,336,411 2 bp→AT coding (83‑84/1260 nt) murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,367,944 G→A A40T (GCG→ACG)  yhcG → DUF1016 domain‑containing protein YhcG
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,449,779 G→A S35S (AGC→AGT rpsC ← 30S ribosomal subunit protein S3
RA 3,468,768 C→T W362* (TGG→TGA chiA ← endochitinase
RA 3,474,425 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,543,877 G→A P237P (CCG→CCA rpnA → recombination‑promoting nuclease RpnA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,629,921 C→T E228K (GAG→AAG)  yhiI ← putative membrane fusion protein YhiI
RA 3,682,515 C→T M311I (ATG→ATA dctA ← C4 dicarboxylate/orotate:H(+) symporter
RA 3,696,909 G→A G151E (GGA→GAA)  bcsE → c‑di‑GMP‑binding protein BcsE
MC JC 3,708,467 Δ168 bp [proK] [proK]
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,761,230 G→A S7F (TCC→TTC)  selA ← selenocysteine synthase
RA 3,771,979 G→T intergenic (‑235/‑302) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
JC 3,822,651 (CAGGAT)1→2 coding (252/2109 nt) spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,823,162 C→T H255Y (CAC→TAC)  spoT → bifunctional (p)ppGpp synthase/hydrolase SpoT
RA 3,869,637 C→A L87L (CTC→CTA cbrA → colicin M resistance protein
RA 3,878,536 G→A Y528Y (TAC→TAT gyrB ← DNA gyrase subunit B
RA 3,883,678 G→A P18S (CCA→TCA)  dnaA ← chromosomal replication initiator protein DnaA
RA 3,912,074 G→A S532F (TCC→TTC)  glmS ← L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
RA 3,931,069 C→T E9K (GAA→AAA)  ravA ← regulatory ATPase RavA
RA 3,955,304 C→T R609R (CGC→CGT ilvD → dihydroxy‑acid dehydratase
RA 3,988,115 C→T D318N (GAT→AAT)  hemX ← PF04375 family protein HemX
RA 3,993,531 T→C R793R (CGT→CGC cyaA → adenylate cyclase
RA 3,996,049 T→A L156* (TTG→TAG)  yigA → DUF484 domain‑containing protein YigA
RA 4,011,802 C→T P243S (CCG→TCG)  bioP → biotin transporter
RA 4,031,491 A→T K111* (AAA→TAA)  pepQ → Xaa‑Pro dipeptidase
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,107,621 +G coding (70/963 nt) pfkA → 6‑phosphofructokinase 1
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,154,371 G→A A159A (GCC→GCT argE ← acetylornithine deacetylase
RA 4,161,248 G→T G61V (GGC→GTC)  fabR → DNA‑binding transcriptional repressor FabR
RA 4,164,123 C→G A162G (GCA→GGA)  btuB → cobalamin outer membrane transporter
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,226,699 G→A G958R (GGG→AGG)  metH → cobalamin‑dependent methionine synthase
RA 4,249,009 Δ1 bp coding (1039/1341 nt) lamB → maltose outer membrane channel/phage lambda receptor protein
RA 4,282,332 (A)5→6 coding (744/1293 nt) yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,392,997 G→A V357V (GTC→GTT queG ← epoxyqueuosine reductase
RA 4,393,552 G→A F172F (TTC→TTT queG ← epoxyqueuosine reductase
RA 4,396,373 C→T T103T (ACC→ACT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,396,772 T→C F236F (TTT→TTC amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,425,688 C→A H57N (CAC→AAC)  priB → primosomal replication protein N
RA 4,425,696 C→A N59K (AAC→AAA priB → primosomal replication protein N
RA 4,499,661 T→C intergenic (+161/‑1599) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,542,725 (T)6→5 intergenic (+92/‑390) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,630,375 G→A R50C (CGC→TGC)  ettA ← energy‑dependent translational throttle protein EttA
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211464 15188 39 [34] [34] 35 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1383143 1404628 21486 89 [0] [0] 79 [ycjW]–abgA [ycjW],ycjX,ycjF,tyrR,tpx,ycjG,mpaA,ymjC,ymjD,ycjY,pgrR,mppA,ynaI,insH4,ynaJ,uspE,fnr,ogt,abgT,abgB,abgA
* * ÷ NC_000913 1978495 1979270–1978503 9–776 113 [0] [3] 131 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423882–3424234 3424457–3424238 5–576 36 [34] [34] 39 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3762499–3764074 3765102–3764079 6–2604 35 [34] [34] 35 rhsA rhs element protein RhsA
* * ÷ NC_000913 3932009 3938982 6974 118 [0] [0] 122 [kup]–[rbsR] [kup],rbsD,rbsA,rbsC,rbsB,rbsZ,rbsK,[rbsR]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1313982107 (1.010)37 (0.350) 31/596 2.4 25.7% intergenic (‑319/‑38) yciC/ompW putative inner membrane protein/outer membrane protein W
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 13831420 (0.000)89 (0.840) 59/598 0.5 100% coding (820/999 nt) ycjW DNA‑binding transcriptional repressor YcjW
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 1397238NA (NA)113 (1.060) 71/598 0.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1404629 =0 (0.000)79 (0.740) 55/598 0.7 100% intergenic (‑64/‑112) abgA/abgR p‑aminobenzoyl‑glutamate hydrolase subunit A/putative LysR‑type DNA‑binding transcriptional regulator AbgR
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1467910 =NA (NA)46 (0.430) 39/598 1.6 53.5% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 1657525 40 (0.380)intergenic (‑68/‑40) ynfC/ynfD PF06788 family lipoprotein YnfC/DUF1161 domain‑containing protein YnfD
* ? NC_000913 1657521 =42 (0.400)43 (0.400) 31/598 2.4 50.6% intergenic (‑64/‑44) ynfC/ynfD PF06788 family lipoprotein YnfC/DUF1161 domain‑containing protein YnfD
?NC_000913 = 2070271 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 1979271 =3 (0.030)128 (1.200) 76/598 0.1 97.7% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)56 (0.530) 38/598 1.7 55.5% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 2819246 = 45 (0.420)intergenic (‑100/+135) csrA/alaS carbon storage regulator/alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
* ? NC_000913 2288919 =NA (NA)33 (0.310) 31/598 2.4 24.4% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3128122 102 (0.960)intergenic (‑101/‑150) glcD/glcC glycolate dehydrogenase, putative FAD‑linked subunit/DNA‑binding transcriptional dual regulator GlcC
* ? NC_000913 = 2290113NA (NA)53 (0.500) 43/596 1.3 53.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 2819249 46 (0.430)intergenic (‑103/+132) csrA/alaS carbon storage regulator/alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
* ? NC_000913 = 3187426NA (NA)118 (1.110) 76/596 0.1 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 3932008 0 (0.000)coding (693/1869 nt) kup K(+):H(+) symporter Kup
* ? NC_000913 3938983 =0 (0.000)122 (1.150) 76/598 0.1 100% coding (757/993 nt) rbsR DNA‑binding transcriptional dual regulator RbsR
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region