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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 29,486 | 0 | C | A | 47.8% | 16.4 / 17.0 | 23 | intergenic (+291/‑165) | dapB/carA | 4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl‑phosphate synthetase small subunit |
* | NC_000913 | 4,027,436 | 0 | T | G | 40.9% | 12.7 / 18.7 | 22 | intergenic (+208/+173) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 4,027,423 | 0 | T | C | 40.0% | ‑2.5 / 20.1 | 20 | intergenic (+195/+186) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 4,296,268 | 0 | T | C | 38.7% | 20.4 / 21.3 | 31 | intergenic (+474/+168) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 4,379,638 | 0 | G | A | 38.1% | 53.0 / 14.8 | 42 | F45F (TTC→TTT) | frdC | fumarate reductase membrane protein FrdC |
* | NC_000913 | 3,760,384 | 0 | A | C | 31.1% | 41.2 / 13.4 | 45 | V289G (GTG→GGG) | selA | selenocysteine synthase |
* | NC_000913 | 2,851,654 | 0 | T | G | 30.9% | 111.1 / 21.2 | 69 | G218G (GGT→GGG) | hypB | hydrogenase isoenzymes nickel incorporation protein HypB |
* | NC_000913 | 2,984,718 | 0 | G | C | 29.8% | 90.4 / 13.8 | 52 | P292R (CCG→CGG) | yqeF | putative acyltransferase |
* | NC_000913 | 1,174,605 | 0 | T | C | 29.2% | 126.3 / 10.4 | 68 | G187G (GGA→GGG) | ycfT | inner membrane protein YcfT |
* | NC_000913 | 4,027,448 | 0 | G | A | 28.6% | 34.5 / 11.9 | 21 | intergenic (+220/+161) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 1,174,626 | 0 | G | A | 27.7% | 124.7 / 16.1 | 66 | F180F (TTC→TTT) | ycfT | inner membrane protein YcfT |
* | NC_000913 | 4,027,449 | 0 | T | G | 27.3% | 37.2 / 11.2 | 22 | intergenic (+221/+160) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 2,185,199 | 0 | T | G | 25.4% | 135.0 / 11.1 | 72 | T35P (ACC→CCC) | thiM | hydroxyethylthiazole kinase |
* | NC_000913 | 1,867,714 | 0 | T | A | 23.2% | 128.0 / 10.6 | 56 | I269I (ATT→ATA) | yeaG | protein kinase YeaG |
* | NC_000913 | 3,375,528 | 0 | C | A | 23.2% | 121.0 / 11.2 | 56 | D220E (GAC→GAA) | dcuD | putative transporter DcuD |
* | NC_000913 | 4,027,458 | 0 | A | . | 22.7% | 50.1 / 14.1 | 22 | intergenic (+230/+151) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 4,027,460 | 0 | A | G | 22.7% | 42.0 / 10.3 | 22 | intergenic (+232/+149) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
* | NC_000913 | 2,262,931 | 0 | C | T | 20.5% | 185.4 / 10.6 | 78 | A189T (GCC→ACC) | fruB | fructose‑specific PTS multiphosphoryl transfer protein FruB |
* | NC_000913 | 2,395,439 | 0 | A | C | 20.0% | 182.5 / 11.2 | 75 | V153G (GTT→GGT) | nuoJ | NADH:quinone oxidoreductase subunit J |
* | NC_000913 | 4,027,462 | 1 | . | G | 20.0% | 65.5 / 19.2 | 25 | intergenic (+234/+147) | fre/fadA | NAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 125819 | 47 (0.780) | 3 (0.060) | 3/442 | 5.3 | 6.8% | coding (125/1893 nt) | aceF | pyruvate dehydrogenase, E2 subunit |
? | NC_000913 | 126432 = | 40 (0.740) | coding (738/1893 nt) | aceF | pyruvate dehydrogenase, E2 subunit |