breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4860CA47.8% 16.4 / 17.0 23intergenic (+291/‑165)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl‑phosphate synthetase small subunit
*NC_0009134,027,4360TG40.9% 12.7 / 18.7 22intergenic (+208/+173)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,027,4230TC40.0% ‑2.5 / 20.1 20intergenic (+195/+186)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,296,2680TC38.7% 20.4 / 21.3 31intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,379,6380GA38.1% 53.0 / 14.8 42F45F (TTC→TTTfrdCfumarate reductase membrane protein FrdC
*NC_0009133,760,3840AC31.1% 41.2 / 13.4 45V289G (GTG→GGG) selAselenocysteine synthase
*NC_0009132,851,6540TG30.9% 111.1 / 21.2 69G218G (GGT→GGGhypBhydrogenase isoenzymes nickel incorporation protein HypB
*NC_0009132,984,7180GC29.8% 90.4 / 13.8 52P292R (CCG→CGG) yqeFputative acyltransferase
*NC_0009131,174,6050TC29.2% 126.3 / 10.4 68G187G (GGA→GGGycfTinner membrane protein YcfT
*NC_0009134,027,4480GA28.6% 34.5 / 11.9 21intergenic (+220/+161)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009131,174,6260GA27.7% 124.7 / 16.1 66F180F (TTC→TTTycfTinner membrane protein YcfT
*NC_0009134,027,4490TG27.3% 37.2 / 11.2 22intergenic (+221/+160)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009132,185,1990TG25.4% 135.0 / 11.1 72T35P (ACC→CCC) thiMhydroxyethylthiazole kinase
*NC_0009131,867,7140TA23.2% 128.0 / 10.6 56I269I (ATT→ATAyeaGprotein kinase YeaG
*NC_0009133,375,5280CA23.2% 121.0 / 11.2 56D220E (GAC→GAAdcuDputative transporter DcuD
*NC_0009134,027,4580A.22.7% 50.1 / 14.1 22intergenic (+230/+151)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009134,027,4600AG22.7% 42.0 / 10.3 22intergenic (+232/+149)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase
*NC_0009132,262,9310CT20.5% 185.4 / 10.6 78A189T (GCC→ACC) fruBfructose‑specific PTS multiphosphoryl transfer protein FruB
*NC_0009132,395,4390AC20.0% 182.5 / 11.2 75V153G (GTT→GGT) nuoJNADH:quinone oxidoreductase subunit J
*NC_0009134,027,4621.G20.0% 65.5 / 19.2 25intergenic (+234/+147)fre/fadANAD(P)H‑flavin reductase/3‑ketoacyl‑CoA thiolase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12581947 (0.780)3 (0.060) 3/442 5.3 6.8% coding (125/1893 nt) aceF pyruvate dehydrogenase, E2 subunit
?NC_000913 126432 = 40 (0.740)coding (738/1893 nt) aceF pyruvate dehydrogenase, E2 subunit