Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,718	0	G	C	29.8%	90.4 / 13.8	52	P292R (CCG→CGG)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (7/26);  new base C (14/0);  total (21/31)
Fisher's exact test for biased strand distribution p-value = 3.40e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCA  >  NC_000913/2984477‑2984961                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                             tttGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCAATTc                                                                                                                                                                                                                                                     >  1:234146/1‑251 (MQ=255)         catgaCGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCTCCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCATGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAAt                                                                                                                                                                                                                                            <  2:459065/247‑1 (MQ=255)             aCGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGcc                                                                                                                                                                                                                                         <  1:444149/250‑1 (MQ=255)                tttCACCATTTCATGAACCAGAGAAACCAGGATTCGGCCACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCCGACACCCgcgcccatacccccccagccgggc                                                                                                                                                                                                                                      >  2:279662/1‑231 (MQ=255)                                     agaAACCATGATTCGGCAACCGGAAGAGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGAGGGCATACCCCCGCGCCATTCC‑CCTCAATGCC‑g‑gcaccacccccacaccggcaaaat                                                                                                                                                                                                              >  1:162429/1‑228 (MQ=255)                                     agaAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACACCCGCGCAAATCC‑CATCAATGCC‑G‑GGTCTACACCCACA‑CT‑GGCCAAt                                                                                                                                                                                                             >  1:231239/1‑250 (MQ=255)                                      gaAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGCGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTTCAACGGCGCAACTTC‑CATCAAAGAC‑G‑GGGCTACACCAAAG‑CTAGGCAAAtc                                                                                                                                                                                                            >  1:507102/1‑250 (MQ=255)                                              gATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGc                                                                                                                                                                                                   <  1:9896/251‑1 (MQ=255)                                                 tCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCgg                                                                                                                                                                                                 <  1:102814/250‑1 (MQ=255)                                                    gcacccggaagtgctaaTCGGGTGACCGAGTGCGACGGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTTTGCAGCAAACGCGTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCTCATTCTTCTGAGCAATTCC‑GCGCACACCC‑A‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGc                                                                                                                                                                                               <  2:148864/233‑1 (MQ=255)                                                         cGGAAGCGCATATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGCCCCGACGCTCATCCCACGCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATTCACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACg                                                                                                                                                                                       <  2:334326/251‑1 (MQ=255)                                                          ggAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCAACCCCCGCGCCATTTC‑CCTCAAATCC‑G‑GGTTTACACCCACC‑CC‑GGCAAAAGCGCGGAAGCGGGGCAGC‑ACgg                                                                                                                                                                                      >  1:213160/1‑251 (MQ=255)                                                           gAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACggg                                                                                                                                                                                     <  1:372971/251‑1 (MQ=255)                                                                      cGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACg                                                                                                                                                                          <  1:298006/251‑1 (MQ=255)                                                                           gACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGt                                                                                                                                                                     <  1:428378/251‑1 (MQ=255)                                                                               gAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCCTACACCGGCGCAATTCC‑CATCCATGCC‑G‑GGTCTACACCAACA‑CC‑GGCAAATGCCCGAAAGCGGGCCAGC‑CCGGGGAAAATCAACTCTCGTggct                                                                                                                                                                 >  1:53785/1‑248 (MQ=255)                                                                                aGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCttt                                                                                                                                                                <  1:122686/251‑1 (MQ=255)                                                                                      aTCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCGCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTc                                                                                                                                                        <  1:422239/251‑1 (MQ=255)                                                                                      aTCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCAGTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CAGCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTc                                                                                                                                                        <  1:415431/251‑1 (MQ=255)                                                                                            ccaccaTTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCtcat                                                                                                                                                  <  1:279662/251‑1 (MQ=255)                                                                                                   tgacattgacCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCGATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTcatcatcat                                                                                                                                            <  2:231239/250‑1 (MQ=255)                                                                                                             ccGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTg                                                                                                                                 <  1:171480/251‑1 (MQ=255)                                                                                                                    ccctCCCACTCAAGCATCTTGCCAACCCAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACGCCTGCGCAATTCC‑CAGCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATc                                                                                                                          <  2:74170/248‑1 (MQ=255)                                                                                                                    ccagcccACTCAAGCATCTTGCCAACCGAAATCGCCTGGGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACTCCGTCGCAATTCC‑CATCAAAGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCTCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATc                                                                                                                          <  2:180301/247‑1 (MQ=255)                                                                                                                          cACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCAATTCC‑CATCAATGCC‑G‑GATCTTCACCAACG‑CT‑GGCAAAAGCGCGAAAGCGGGCCCGC‑CAGGGTAAATTCAAAGCTCGTGCCTT‑TGC‑TTCGCTCATCATCATTAACCCAGCTGCGCCATCGttttt                                                                                                                    >  1:138355/1‑249 (MQ=255)                                                                                                                          cACTCAAGCATCGTGCCAACCGAAAGCGCCTGTGCACCAAACTCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGTCAACGGCGGGTCGCATTTATTTTAATGATACGGCGCCCA‑‑CC‑GAGATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTAt                                                                                                                    <  2:357708/251‑1 (MQ=255)                                                                                                                             tCAAGCATCTTGCCAACCGAAAGCGCCTGTGCCGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCCATTCC‑CCTCAATGCC‑G‑GATCTACACCAACG‑CT‑GGGCAATGCGCGAAAGCGGGCCAGC‑ACGGGGAAATTTAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTTTggg                                                                                                                 >  1:370924/1‑250 (MQ=255)                                                                                                                               aaGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGgatg                                                                                                               <  1:432928/251‑1 (MQ=255)                                                                                                                                  cATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGatgatg                                                                                                            <  1:59705/251‑1 (MQ=255)                                                                                                                                       tGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACCTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGc                                                                                                           <  1:322897/247‑1 (MQ=255)                                                                                                                                       gtccAACCGAAAGCGCCTGTGCAGCCAACGCTTCATTGGCCTCGATAAGATCGCCTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGTGTCGCTTTTTTTTTAATGATACG‑GAGCA‑CACC‑GAGATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCAt                                                                                                         <  2:501752/247‑1 (MQ=255)                                                                                                                                                   gcgcCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGAGCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACTCCGGCGCAATTCG‑CCGCACAGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTcaca                                                                                           <  2:268120/251‑1 (MQ=255)                                                                                                                                                                aaaCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGGCGCATACACCCGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑TGCAAAAGCGCGAATGCGGGCCAGC‑ACGGGGAAAATCAACGCCCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCCGCCGCGCCCTCGTTTTTGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑At                                                                             >  1:243147/1‑251 (MQ=255)                                                                                                                                                                  aCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCCATTCC‑CCTCAATGCC‑G‑GATCCCCACCAACC‑CC‑GGCCAATACGCGAATACGGGCCAGC‑ACGGGGAAAATCAACACTCGCGCTTT‑TTC‑TTCGCCCATCCACATTATCACAACTGCCCCCTCGTTTATAGAGGATGCATTACCCCCTGTCACACAACCAAGAccatt                                                                             >  2:507907/1‑246 (MQ=255)                                                                                                                                                                  aCGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCAATTCC‑CATCCATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAAAGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCCCGTGCTTT‑TGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCCTCGTTTATGGATGATGCCTTACCCCCCGTCACAGAACCCAGACT‑ATc                                                                            >  1:294718/1‑250 (MQ=255)                                                                                                                                                                                tcgctactatcgccttcgtccAACTGCCAGCCTACGCGCGCCAGGCAACGGCGGGTCGCTTACTCCGGCTCGATTCC‑CATCAATCCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTa                                                              <  2:238078/231‑1 (MQ=255)                                                                                                                                                                                        aTCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGCCGCATACACCGGCGCAATTTC‑CCTCAATGGC‑G‑GGTCCAAACCCACGGCT‑GGCAA‑TGCGCGCAAGCGCGGCAGC‑CCGGGGAAAATAAAAGCTCGGGGTTT‑GGCCATC‑CTCATCACAATTACCCCAGCTGCGGCATTGGTTTTGGATGATGCATTTCACCCCGTCACAGAACAGAGGCG‑ATCAAAAGAAGGGTTTaaaacggct                                                      >  1:360219/1‑244 (MQ=255)                                                                                                                                                                                                ccgCCAACTGCCAGCCTATCCGCGCCAGGCAACGGCTGGTCGCATACATCGGCGCGATACC‑CAGCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGAGGATGCATTACCCGCTGTCACAGATCCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCt                                             <  2:372348/249‑1 (MQ=255)                                                                                                                                                                                                 aGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCCTACACCCGCGCCCATCC‑CCTCCATGCCCG‑GA‑CTACACCAACG‑CT‑GGCAAATCCGCGCATGCGGGGCAAC‑ACGGGGTAATTCCACGCCCGTGCTTT‑TGC‑GTCGCTCCTCATCATTACCCCACCCGCGCCCTCGTTTATGGTGGATGCGTTACCCCCTCTCCCAGAACCCAGACTCAACAAA‑GAAGGATTTTAATAGGCTAAGCCTTCtt                                            >  2:111235/1‑250 (MQ=255)                                                                                                                                                                                                         gcacgCCTACACGCCCCATGCATCGGCGGGTCGCATACACCGGGTCAATTCC‑CAGCAAGGCC‑G‑GATCTACACCAACG‑CG‑GGCAAATGCGCGAATGCGGGCCAGC‑TCGGGTAAATTCAACGCCCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCg                                    <  2:162429/247‑1 (MQ=255)                                                                                                                                                                                                         gCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCCGCGCAAATCC‑CCTCAATGCC‑G‑GATCTACACCCACG‑CT‑GGCCAATGCGCGAATGCGGGCCCGC‑ACGGGGAAATTTAACGCTCGTGCTTT‑TGC‑TTCGCTCCTCATCATTACCGCAGCCGCGCCCTCGTTTATGGATGATGCATTACCCCCCGTCACAGAACCCAGACT‑ATCAAATGAAGGATTTAAAAGGGCTAAGCCTTCTTCGCTGGCg                                    >  1:48725/1‑251 (MQ=255)                                                                                                                                                                                                           cAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACTCCTGAATGATACG‑CCGCCATCCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTc                                  <  2:230581/251‑1 (MQ=255)                                                                                                                                                                                                              ccTACACGCTCCAGGCAACGGCGGGTCGCCAACACCCGCGCCATTCC‑CCTCAATACC‑C‑GGGCTCCACCCACC‑CC‑TGCCAATGCGCGAATGCGGGCCAGCCAAGGG‑AAAATCAACGCCCGGGCTTT‑TGC‑TTCGCTCCTCATCATTACCGCCACTGCCCCACCGGTTATTGGTGGTGCATTACCCCCTGTCCCCGAACCGAGACT‑ATCAAAAGAAAGATTTAAACGGGCTAAGACTTCTGCGCTGGCGTCAGt                               >  1:192850/1‑251 (MQ=255)                                                                                                                                                                                                              ccTACACGCTCCAGGCAACGGCGGGTCCCATACACCCGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAAAGCGGGCCAGC‑ACGGGGAAATTCAACGCTCGTGCTTTTGCC‑TTC‑CTCATCCTCATTACCGCACATCCGCCATCCTTTATGGATGATGCATTACCCCCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAgg                               >  1:498837/1‑250 (MQ=255)                                                                                                                                                                                                                 acacGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGc                             <  1:10223/250‑1 (MQ=255)                                                                                                                                                                                                                                cggcggGTCGCATACCCCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGt             <  1:508774/251‑1 (MQ=255)                                                                                                                                                                                                                                  gcggGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTAt           <  1:377112/251‑1 (MQ=255)                                                                                                                                                                                                                                      gTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGCGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGCGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATcaac       >  2:376039/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTTCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATcaacaa     <  1:98775/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacc   >  1:386585/1‑250 (MQ=255)                                                                                                                                                                                                                                            tACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca  >  2:77094/1‑250 (MQ=255)                                                                                                                                                                                                                                            tACACCGGCGCAATTCC‑CATAAATGCC‑G‑GATCTACACCCACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAAcaacca  >  2:456400/1‑250 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                             TTTGCGGGCATTACGTTTCACCATTTCATGAACCAGAGAAACCAGGATTCGGCAACCGGAAGCGCCTATCGGGTGACCGAGTGCGATCGCGCCACCATTGACATTGACCCGACGCTCATCCCACTCAAGCATCTTGCCAACCGAAAGCGCCTGTGCAGCAAACGCTTCATTAGCCTCGATAAGATCGACTTCAGCCAACTGCCAGCCTACACGCTCCAGGCAACGGCGGGTCGCATACACCGGCGCAATTCC‑CATCAATGCC‑G‑GATCTACACCAACG‑CT‑GGCAAATGCGCGAATGCGGGCCAGC‑ACGGGTAAATTCAACGCTCGTGCTTT‑GGC‑TTCGCTCATCATCATTACCGCAGCTGCGCCATCGTTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACT‑ATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCA  >  NC_000913/2984477‑2984961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑