| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,185,199 | 0 | T | G | 25.4% | 135.0 / 11.1 | 72 | T35P (ACC→CCC) | thiM | hydroxyethylthiazole kinase |
| Reads supporting (aligned to +/- strand): ref base T (13/40); new base G (16/2); total (30/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAT > NC_000913/2184949‑2185446 | gATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑AAAAGG‑t > 1:14032/1‑251 (MQ=255) aTAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑tt < 1:93926/251‑1 (MQ=255) cATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGACCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGGTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAACG‑TTTGcgcacac > 2:337360/1‑245 (MQ=255) gACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑GAAAGG‑AGTGCACCAcatcc > 2:475597/1‑250 (MQ=255) aCAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCAcatcat > 1:340136/1‑251 (MQ=255) ggtcggcgATAATCGGGCGCCCCCGCCGCTACTGGATCAAGCGTCGAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGTTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTcgg < 2:518136/248‑3 (MQ=255) ggcggcGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCCCATCATTGGTCATg > 2:356331/1‑251 (MQ=255) ggcgATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGATAAGGG‑TTTGcaccccccttttgggcgtgcg > 2:212317/1‑234 (MQ=255) gcgATAATCGTGCTCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGTCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCTCTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGt < 1:323698/251‑1 (MQ=255) gATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCCTTTGTCATGCAgggc > 1:426113/1‑248 (MQ=255) aaTCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATTATTGGGCATGCCGTgagc > 1:203364/1‑247 (MQ=255) acccaccGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑t < 1:84934/251‑1 (MQ=255) cccaccGCTACTGGATCAAGCGTCCAGGGTTGTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTTCACA‑AGAGGGGAA‑Tg < 2:448629/251‑1 (MQ=255) cccaccGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCCTTGGTCATGCAGGGCACA‑AGAGGGGGA‑Tg > 1:57278/1‑251 (MQ=255) accGCTACTGGACCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGATGGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATGGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTg < 2:125603/251‑1 (MQ=255) cGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTg < 1:131052/231‑1 (MQ=255) aCTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCCGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATGGGTCACGCAGCGCACA‑AGAGGGGAA‑TGTTGGTGa < 2:465514/249‑1 (MQ=255) aTCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTTGTCATGCAGTGCACA‑AGAGGGGGA‑TGTTGGTGAAAAAggg > 1:320501/1‑250 (MQ=255) cAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGgtg < 2:390495/250‑1 (MQ=255) aGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCTAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTaa < 1:89109/251‑1 (MQ=255) aGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTaa < 1:523357/251‑1 (MQ=255) gCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAAc < 1:495228/251‑1 (MQ=255) gCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAAc < 1:465818/251‑1 (MQ=255) gTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCCTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAAcgc < 1:501966/251‑1 (MQ=255) ccAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGt < 2:178239/251‑1 (MQ=255) tttGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTTCACCACATCATTGGTCATGCCGGGCACA‑AGAGGGGGA‑TGTTGGGGGAAAAGGTGTAACGCGGGCGCAGATTGCGCTGaaac > 1:172150/1‑249 (MQ=255) gcttgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGGGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCca < 2:122741/250‑1 (MQ=255) tgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCCCCACATCATTGGTCATGCCGGGCACA‑AGAGGGGAA‑TGTTGGGGAAAAAGGGGTAACGCGTGCGCCGATTGCGCTGAACCcagccg > 1:242324/1‑249 (MQ=255) ctCAACGGCAGCACGCATCGACTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAAG‑TTTTGAACACATCCATGGTTATGCCGTGC‑CACAGAGGGGGA‑TGATGGTGAAAAAAGGGGAACGCGGGCGCAGAtttgggtgaaccccacaggg > 2:350945/1‑233 (MQ=255) cAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAACCTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGTTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGt < 2:437513/249‑1 (MQ=255) cAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTTGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGt < 2:385775/249‑1 (MQ=255) cAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTc < 2:268325/250‑1 (MQ=255) cagcaCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGc < 2:426113/251‑1 (MQ=255) aCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGGGTTTG‑ACCACATCATTAGTTATGCAGGGCCCA‑AGGGGGGGA‑TTTTGGTGAAAAAGGAGGAACGCGTGAGCAGATTGCGCTGAACCCCACAGGTGGATTTGCATat > 1:25828/1‑250 (MQ=255) gCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGtt < 2:372862/251‑1 (MQ=255) cATCGCCTGGGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGttt < 2:25220/251‑1 (MQ=255) cATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGttt < 1:83225/251‑1 (MQ=255) cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TACAGG‑GTTGCACCACATCCTTGGTCCAGCCGTGCGCA‑CGAGGGGGA‑TGGTGGTGTGAAAAGTGTGAAGCGGGCGCCGAATGCGCGGGAACCAGCAGGGCGGCGTGCCTAGTTTGCTCCTGCCaca > 2:200132/1‑248 (MQ=255) ggCTGCGTCAGTGTGCCAACGTTAAGCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGt < 2:96189/251‑1 (MQ=255) gCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTgaa‑g < 1:217640/251‑1 (MQ=255) aGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCCTTGGTCATGCCGTGCACC‑AGAGGGGGA‑AGGTGGTGAAAAAGGTGTAACGCGTGCGCCGATTGCGCTGAACCCAGCAGGGCGGCTTGCATAGTTTTCTCCTGCCATAACGTGAA‑GAAGc > 1:319159/1‑250 (MQ=255) tgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCCCCACATCATTGGGCATGCAGTGCACA‑AGAGGGGGA‑TGTTGGTGGAAAAGGAGGAACGCGGGCGCAGATTGCGGTGAACCCAGCAGGGCGACTTGGATAGTTTGTTCCTGCCCTAACGTGAA‑GAAGCAATg > 2:183582/1‑250 (MQ=255) cAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑t < 1:92504/251‑1 (MQ=255) cAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGGA‑TGTTGGTGAAAAAGGTGTAACGCGGGGGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTTCTCCTGCCATAACGTGAA‑AAAGCAATGACC‑t > 1:411618/1‑251 (MQ=255) cGTTAATCGACAAGGCACTTGGGATAGCCGCACACTGAATGGCCTATACGGTTGCGATAACCATCGCTGGCGATGCACCGAGCGCCAGAAAGGTATTGGCGG‑TAAAGG‑TGTGCACCGCATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTGGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGAAC‑tggt < 2:209580/251‑1 (MQ=255) caacaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCGTCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGt < 1:186883/251‑1 (MQ=255) acaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGGA‑TGTTGGGGAAAAAGGGGTAACGCGTGCGCAGATTGCGCTGGACCCAGCAGGTCGGCTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTgg > 1:45480/1‑250 (MQ=255) acaaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGa < 2:290219/251‑1 (MQ=255) aCTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGGA‑TGTTGGTGAAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAAAGACC‑TGGTGGGCCGTGACTTcccc > 1:79506/1‑250 (MQ=255) ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTAc < 1:198080/250‑1 (MQ=255) ggCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACg < 2:476742/251‑1 (MQ=255) aaaCTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACAACATTGGTCATGCAGTGGACC‑AGAGGGGGA‑TGGTGGTGAAAAAAGTGTAAAGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGGCTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCAATATcc > 2:275417/1‑251 (MQ=255) actgactgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGTCCCCCACCATTGGTTATGCAGTGGACA‑AGAGGGGGA‑TGTTGGGGAAAAAGGGGTAACGCGTGCGCCGATTGTGCTGAACCCAAAAGGGCGACTTGCCTAGTTTGCTCCCGCCATAACGTGAA‑GAAGCAATTACC‑CGGGGGTCCGTGACTTCCCTAccatgggcttatcca > 2:400556/1‑236 (MQ=255) gactgGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGa < 1:337360/249‑1 (MQ=255) cGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACggg < 2:480528/251‑1 (MQ=255) cGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑CAAAGG‑GTTGCGCCACATCAATGGACATACCGGGCACA‑AAAGGGGGA‑TGTTGGGTAAAAAGGGGTAAAGCGTGCGCAGCATGCGCTGAAACCAGCAGGGAGACTTTCATAGTTTGCTCCTGCCATAACCTGGA‑AAAGCAAATACC‑TGGTGGTCCGTGACTTCCCTACGCCGGCATTAtaccgaaaaggggatacggg > 1:488568/1‑232 (MQ=255) cGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑GGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTGCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTAttt < 2:307584/250‑1 (MQ=255) gATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑AAAAAG‑TTTGCACCACCACATTGGGCATGCAGGGCACA‑AGAGGGGGA‑TGGTGGGGGAAAAGGGGTAACGCGGGCGCAGATTGGGCTGAACCCAGCCGGTCGGCTTTCATTGATTGCTCCTGCCATAACAGGGA‑GAAACCAAGACCCTGGTGG‑CCGTGACTTCCCTTCCCTGGCATTATTCAGATCCGGTGGTAAGGGGAttttt > 2:524353/1‑249 (MQ=255) aTAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTtctc < 2:319159/251‑1 (MQ=255) tAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTTCACCACATCATTTGTCAATCCGGGGACA‑AGGGGGGGA‑TGTTGGGGAAAAAGGGGGTACGCGTGGGGGGATTGCGCTGAACCCAGCAGGTCGGCTTGCATTGTTTTCTCCTGCCATAAAGTGAAAGAA‑CAATGACC‑TGGGGGTGCGTGACTTCCCTACGCTGGCATTATCCCGATCAGGTGGTACGGGTAttttccc > 2:241696/1‑247 (MQ=255) tAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGGACA‑AGAGGGGGA‑TGTTGGGGAAAAAGGTGGAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGCCTTCCCTACGCTGGCATTATTCCGATCAGGGGGTACGGGGATTtctc > 1:225610/1‑250 (MQ=255) aTCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGTA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCtt < 2:320501/251‑1 (MQ=255) cgcCAGCAAGGTATTGGCGG‑TAAAGG‑GTTGCACCCCATCATTGGTCATGCCGTGCACA‑AGAGGGGGA‑TGTTGGGGAAAAAGGGGTAACGCGCGCGCCGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGGGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGGGATACGGGGATTTCTCAGCCTTCACGCAGAAGGGCACCCCgg > 1:473483/1‑250 (MQ=255) cAGCAAGGTATTGGCGG‑GAAAGG‑TTTGCACCAAAGCATTGGACATGCGGTGCACA‑AGAGGGGAA‑TGTTGGGGAAAAAGGTGTAACGCGTGCGCAAATTGCGCTGAGCCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGTCC‑TGGTGGTCCGTGACTTCCCTCCGCTGGCATGAACCAGATCAGGTGATGCGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAggc < 2:61975/251‑3 (MQ=255) aGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑GGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTTCATTGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCg < 1:109062/251‑1 (MQ=255) cAAGGTATTGGCGG‑TAAAGG‑GTTGCACCACATCATTGGTCATGCCGTGCACA‑AGAGGGGAA‑TGTTGGGGAAAAAGGGGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGtt > 1:148494/1‑251 (MQ=255) agtGGCGT‑TAAAGT‑TGTGAACCACATCATTGGTAATGCAGGGCACA‑AGAGGGGAAATGT‑GGTGAAAAGGTTGTAGGGCGTGGGCAGATTGCTCTGATCCCAGCAGGACTTCTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGTTGGCATTATCCAGAGCAGGTGATACGGGTATTTCGCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGtg < 2:271353/248‑2 (MQ=255) tGGCGG‑TACAGG‑GTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGGGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCg > 2:365299/1‑251 (MQ=255) ggg‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatg < 2:177364/250‑1 (MQ=255) gg‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGAACCCCGAGTCGTTTGGTTGCgatg > 1:356877/1‑250 (MQ=255) aaaGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑CGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTGGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATa < 2:25828/250‑1 (MQ=255) aaGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATaa > 1:470157/1‑250 (MQ=255) aaGG‑GTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGGGCGCAGATTGCGCTGAACCCAGCAGGTCGGCTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGGAGGGCACCCCGAGTCGTTTGGGTGCGATGATaaa < 2:155829/251‑1 (MQ=255) aGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAt < 1:255646/251‑1 (MQ=255) | GATAAAAGTTCATGACAAAAATGGCGGCGATAATCGAGCGCACCCACCGCTACTGGATCAAGCGTCCAGGGTGTTTGAGAGCTTTTTGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGG‑TAAAGG‑TTTGCACCACATCATTGGTCATGCAGTGCACA‑AGAGGGGAA‑TGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAA‑GAAGCAATGACC‑TGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAAT > NC_000913/2184949‑2185446 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |