Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,262,931	0	C	T	20.5%	185.4 / 10.6	78	A189T (GCC→ACC)	fruB	fructose‑specific PTS multiphosphoryl transfer protein FruB
Reads supporting (aligned to +/- strand):  ref base C (46/16);  new base T (0/16);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 3.49e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCCGCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATG  >  NC_000913/2262689‑2263180                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            ttccGCATCCGCGTTCAGCAAGCGGTCAACTTTTTTGTCGAGCAATAAAGCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACGCACCAGCATGGCTGCCGTTGCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑g                                                                                                                                                                                                                                                    <  2:203097/248‑1 (MQ=255) tGCCGCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑g                                                                                                                                                                                                                                                    <  2:176665/250‑1 (MQ=255)   ccGCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACg                                                                                                                                                                                                                                                 <  1:268732/251‑1 (MQ=255)     gCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGCGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACgcg                                                                                                                                                                                                                                               >  2:467592/1‑249 (MQ=255)     gCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGaa                                                                                                                                                                                                                                               <  1:9220/251‑1 (MQ=255)       aTCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCCCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGaa                                                                                                                                                                                                                                               >  1:221467/1‑249 (MQ=255)               tCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTc                                                                                                                                                                                                                                      <  2:70701/250‑1 (MQ=255)                cAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCaa                                                                                                                                                                                                                                    <  1:28266/251‑1 (MQ=255)                       cGGTCAGCTTTATTGTCGAGCAATAAAGCAGCGAGGCGCTTAAGAACCGCGATGGGGTGAGCGTCATTCATCGCCACACTCACCAGCATGACTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGACTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGcccc                                                                                                                                                                                                                             <  1:237269/251‑1 (MQ=255)                         gTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAACGTGGCGTCAACTGcccccc                                                                                                                                                                                                                           >  2:95573/1‑250 (MQ=255)                         gTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCg                                                                                                                                                                                                                           >  1:329055/1‑251 (MQ=255)                            aGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACAACAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCct                                                                                                                                                                                                                        >  1:523309/1‑251 (MQ=255)                                       cGAGCAATAAATCAGCGCGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAcgc                                                                                                                                                                                                             >  2:449130/1‑251 (MQ=255)                                       cGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGAcgc                                                                                                                                                                                                             >  1:229490/1‑251 (MQ=255)                                            aaTAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGc                                                                                                                                                                                                        >  1:453156/1‑251 (MQ=255)                                               aaaTCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGtt                                                                                                                                                                                                     >  1:317792/1‑251 (MQ=255)                                                 accAGCGAGACGCTTAAGACCCGCGATGGGTTGATCGTCATTCATCCCCACCCTCACCAGCATGGCTGCCTTTCCGCC‑GTCCACATAAAAAGCATTTGCCGCCCGGCTT‑ACCGCACTCGCGCTACGCAGATTGCC‑TTGGG‑CGCTATCCCTCAGCCAGATCCCCTGTC‑CGAGATTCAGCGGTTTTTCATTGATGGCTTT‑GGT‑GACGAAAGGGGGGTCAACTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCa                                                                                                                                                                                                   <  1:62698/249‑1 (MQ=255)                                                   cAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAgc                                                                                                                                                                                                 >  1:249633/1‑251 (MQ=255)                                                      cGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGcc                                                                                                                                                                                              >  1:161860/1‑251 (MQ=255)                                                       gagaCGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCGGTTTCGCG‑GTCCACATCAAAAGCATTTGCCGCACGGCTA‑ACCGCAATCGCGCTACGCAGAATGCT‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGCGATTCAGCGGTTGTTAATTGATGGCTTT‑GGT‑GACGAACGGGGCGTCAACCGCCCCCGCCCCtttacgtcgcgcagatttcacctccg                                                                                                                                                                                             >  2:333169/1‑228 (MQ=255)                                                       gagaCGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCt                                                                                                                                                                                             >  1:127982/1‑251 (MQ=255)                                                        agaCGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCCTTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTg                                                                                                                                                                                            >  2:65558/1‑251 (MQ=255)                                                           cGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGa                                                                                                                                                                                           >  2:62849/1‑249 (MQ=255)                                                               taaaacccgcgtgggcctgcttgTCATTCCTCGCCACCCGCCCCAGCATGGCTGCCTTTTCGCC‑TCCCACATCAAAAGCATTTGCCGC‑CGGTTTTACCCAAATCGCGTTCCGCTGTTTGCC‑TTCGG‑CGCTATCGTTCAGCCAGATCCCTTGTC‑CGATTTTCAGCGTTTGTTCATTGTTGGTTTT‑GTT‑GAGGAAAGTGGCGTCACCCCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAagag                                                                                                                                                                                       <  1:333169/227‑1 (MQ=255)                                                               tAAGACCCGCAATGGGATGAACGTCATTCACCGCCACCCACACCAGCATGGCCGC‑GTTTCCCCCGTCCACATAAAAAGCTTTTGCCGCACGGCTT‑CCCGCAACCGCGCTCCGCAGATTCCC‑TTCGG‑CGCTAGCGCTAGGCCAGATCCCCTTTC‑CGAGATTCAGCGGTTGTTCATTGAGGCCTTT‑GGT‑GACGAAAGTGGCTTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGt                                                                                                                                                                                      <  1:467674/250‑1 (MQ=255)                                                                aaGAACCGCGATAGGCTGATCGACATTCATCGCCACACTCACCAGCATGACTCCCGTTTCCCA‑GTCCACATCACAAGCATTCGCCGCACGCCTT‑ACCGCAATCAAGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGCC‑CGAGATTCACCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGCCCCGCAGCGTTCAGCGACTGAAGAGTc                                                                                                                                                                                     >  2:193478/1‑250 (MQ=255)                                                                          aTGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCt                                                                                                                                                                          >  2:389462/1‑251 (MQ=255)                                                                                  aTCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGcgacg                                                                                                                                                                   >  2:375113/1‑250 (MQ=255)                                                                                  aTCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGcgacg                                                                                                                                                                   >  2:449827/1‑250 (MQ=255)                                                                                                ccACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCCCATCAAAACCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGCAATCCAGCGTCAGc                                                                                                                                                    >  2:379997/1‑251 (MQ=255)                                                                                                ccACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGc                                                                                                                                                    >  1:160691/1‑251 (MQ=255)                                                                                                   ccccccccAGCATGGCTGCCGTTTCGCC‑GCCCACAACAAAAGCATTCGCCGCACGGCTT‑ACCGCAACCGCGCTACGCAGATGGCC‑TTCGG‑CGCTATCGCCCAGCCAGATCCCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGTTTT‑GGG‑GACGAAAGTGGCGTCACCGCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCAtt                                                                                                                                                 <  1:361017/245‑1 (MQ=255)                                                                                                    cccccccAGCATGGCTGCCGTTCCCCC‑GCCCACATAAAAAGCTTTGGCCGCCCGGCTT‑ACCGCAACCGCGCTCCGCAGATTGCC‑TGCGG‑CGCTATCGCTCAGCCAGATCCCCTGTC‑CGAGATTCAGCGTTTTTTCATTGATGGTTTT‑GGT‑GACGAAAGGGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCAttt                                                                                                                                                <  1:336991/246‑1 (MQ=255)                                                                                                     cTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTc                                                                                                                                               >  2:25563/1‑251 (MQ=255)                                                                                                      tCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCg                                                                                                                                              >  1:87608/1‑251 (MQ=255)                                                                                                        cccgccATGGCTGCCTTTCCGCC‑GTCAAAAACAAAGGCTTTTGCCCCCCGGCTT‑CCCGCAACCGCGCTCCGCAGATTGCC‑TTCGG‑CGCTATCGCTCACCCAGATACCCTGCC‑CGAGATTCGGCGTTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGGGGCGCCAACTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGtt                                                                                                                                            <  1:474672/246‑1 (MQ=255)                                                                                                        aCCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATAGCTCAGCCAGATACCCTGCC‑CGCGATCCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGCAAGTGGCGCCAACTGCCCCCGCCTCTTTCCGCCGCGCAGCGTCCAGCGCCTGAAGCGTCCGCAGCTCCCCGGCGGCGCTATCCAgactccgcctttcgtt                                                                                                                                            >  2:127933/1‑236 (MQ=255)                                                                                                         ccAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTg                                                                                                                                           >  2:303683/1‑251 (MQ=255)                                                                                                          cAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGt                                                                                                                                          >  1:58990/1‑251 (MQ=255)                                                                                                                 gCTGCCGTTTCGCC‑GTCCACATCAAAAGCTTTGGCCGCGCGGCTT‑ACCGCAACCGCGCTACGCAGATTGCC‑TTCGG‑CGCTACCGCTCAGCCAGATACCCTGTC‑CGAGTTTCAGCGGTTGTTCATTGATGGTTTT‑GGG‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATc                                                                                                                                                              <  1:10802/224‑1 (MQ=255)                                                                                                                 gCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATc                                                                                                                                                              >  2:10802/1‑224 (MQ=255)                                                                                                                    gCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTc                                                                                                                                  >  1:48397/1‑249 (MQ=255)                                                                                                                              gctctcccgatcaaaGCATTTGCCGCACGGTTT‑ACCGCAACCGCGCTACGCA‑TTTGCCCTTCGG‑CCCTATCTCTCAGCCGGATCCCCTGCC‑CGAGATTCGGCGGTTGTTCAGTGATG‑TTTT‑GGTTGACAAAAGGGGCGTCAATACCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCAc                                                                                                                        <  1:386671/238‑1 (MQ=255)                                                                                                                                  ccACATCAAAAGCTTTTGCCGCCCGGCTT‑ACCGCAATCGCGCTCCGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATCCCCTGTC‑CGAGATTCGGGGGTTTTTCATTGATGGCTTT‑GGT‑GACGAAAGGGGCGTCACCTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGc                                                                                                                   <  1:449827/251‑1 (MQ=255)                                                                                                                                  ccACAACAAAAACATGTCCCGCACGGTTT‑CCCGCAGCCGCACCACGCAGATTGC‑‑TCCGGGCGCTATCGCCCTGCCAGATCCCCTGGC‑CGTGATCTAGCGTTTGTTCGTTGAGGTCTTT‑GGT‑GCCGAAAGTGGCTTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTg                                                                                                                    <  1:222075/250‑1 (MQ=255)                                                                                                                                      ataaaaaGCATTTGCCGCACGGCTT‑CCCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTTCCGCTCAGCCAGATCCCCTGTC‑CGAGATTCAGGGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGGGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCtttt                                                                                                               <  1:459377/248‑1 (MQ=255)                                                                                                                                        cAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCg                                                                                                             >  2:269566/1‑251 (MQ=255)                                                                                                                                        aaaaaGCATTTGCCGCACGGCTC‑ACCGCAATCGCGCACCGCAGATTGCT‑TTCGG‑CGCTATCCCTCAGCCAGATCCCCTGTC‑CGAGTTTCGGCGGTTGTTAATTGATGGTTTT‑GGG‑GACGAAGGTGGCGCCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCg                                                                                                             <  1:503992/250‑1 (MQ=255)                                                                                                                                           aaGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGAGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCCGCCTTTCGTTGTCGAGCTTCAGCTGCTCAc                                                                                                                        >  2:386671/1‑237 (MQ=255)                                                                                                                                                   gCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTa                                                                                                   >  1:86998/1‑250 (MQ=255)                                                                                                                                                    ccGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGCTATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAAt                                                                                                 >  2:404854/1‑251 (MQ=255)                                                                                                                                                       cACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATgcg                                                                                              >  2:301172/1‑251 (MQ=255)                                                                                                                                                        aCGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCCCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGCCGCGCAGCGTTCAGCGCCTGAAGAGACAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATgcgc                                                                                             >  1:355826/1‑251 (MQ=255)                                                                                                                                                             tt‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGt                                                                                        >  1:122907/1‑251 (MQ=255)                                                                                                                                                                cccgcagccgccccggccggtttGCC‑TGCGG‑CGCTAGCCCCCAGCCAGATACCCT‑TCGCGAAATTCGGCGGTTGTTAGTGGAGGGTTTT‑GGG‑GACGAGAGGGTCATCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGtt                                                                                       <  2:280744/229‑1 (MQ=255)                                                                                                                                                                         gcgcTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCtgt                                                                             >  2:483095/1‑251 (MQ=255)                                                                                                                                                                               cGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGc                                                                       >  1:304842/1‑251 (MQ=255)                                                                                                                                                                                gcgaaTTGCC‑TTCGG‑CGCCATCCCTCAGCCAAATCCCCTGTC‑CGAGATTCGGCGGTTGTTAATTGAGGGTTTT‑GGT‑GCCGAAGGTGCCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCt                                                                      <  1:58631/247‑1 (MQ=255)                                                                                                                                                                                gCAGATTGCC‑TCGGG‑CCCTACCGCCCAGCCAGTTCCCCTGTC‑GGAGATTCGGCGTTTGTTCATGGATGGTTTG‑GGT‑GACGAAAGTGGCGTCAACTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCt                                                                      <  1:389462/251‑1 (MQ=255)                                                                                                                                                                                 cAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATCCCCTGTC‑CGAGATTCGGGGGTTGTTCTTTGAGGGTTTT‑GGT‑GACAAAAGGGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTg                                                                     <  1:347400/251‑1 (MQ=255)                                                                                                                                                                                     tggCC‑TGCGG‑CGCTCCCGATCAGCAAGATCCCCTGTC‑CGGGTTTCAGGTGTTTTTCATTG‑TGGCTTTTGGG‑GACGAAGGGGGCGTCACCGCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACt                                                                  <  2:434103/248‑1 (MQ=255)                                                                                                                                                                                       gCC‑TTCGG‑CGCTATCGCTCAGCCAGATCCCCGGCC‑CGAGATTCAGCGGTTTTTCATTGATGGTTTT‑GGG‑GACGAAAGGGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCa                                                               <  1:269566/251‑1 (MQ=255)                                                                                                                                                                                        cc‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTc                                                                >  1:366148/1‑249 (MQ=255)                                                                                                                                                                                                 cgcTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTc                                                        >  2:250960/1‑250 (MQ=255)                                                                                                                                                                                                      ccccTCAGCCAGATACCCTCCC‑GGAGATTCAGGGGTTGTCCATTGATGGTTTT‑GGT‑GACGAAAGGGGCGCCAACTCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAAc                                                  <  1:244/248‑1 (MQ=255)                                                                                                                                                                                                        gCTCAGCCAGCTACCCTGTC‑CGAGATTCAGCGTTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACgg                                                >  2:153133/1‑251 (MQ=255)                                                                                                                                                                                                           cagcaaaactcccTGCC‑CGAGATCCGGCGGTTGTTCTTTTAGGGTTTT‑GGT‑GCAGAAGGGGGCGCCACCCGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAat                                             <  2:87608/241‑1 (MQ=255)                                                                                                                                                                                                           cAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAat                                             >  2:491859/1‑251 (MQ=255)                                                                                                                                                                                                                   tACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTc                                     >  1:283086/1‑251 (MQ=255)                                                                                                                                                                                                                      ccttcc‑cGAGATCAAGCGTTTGTTCATTGGTTGCTTT‑GGT‑GACGAAAGTGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGt                                  <  1:228394/246‑1 (MQ=255)                                                                                                                                                                                                                          gtcggagatccggCGGTTGTTCATTGATGCTTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTa                                 <  1:449130/237‑1 (MQ=255)                                                                                                                                                                                                                                    caggtttttttCATTGGTGGTTTT‑GGT‑GGAGAACGTCGCGTCAACCCCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCt                     <  2:16749/242‑1 (MQ=255)                                                                                                                                                                                                                                         gtttttCATTGATGGTTTT‑GGT‑GACAAAAGTGGCGTCACCTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGt                <  1:63273/247‑1 (MQ=255)                                                                                                                                                                                                                                         gttgttCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGt                >  1:112656/1‑251 (MQ=255)                                                                                                                                                                                                                                               cATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAgg          >  1:230921/1‑251 (MQ=255)                                                                                                                                                                                                                                                      ggCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGAAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGACGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGccc       >  2:205548/1‑247 (MQ=255)                                                                                                                                                                                                                                                       gCTTT‑GGT‑GACGAAGGTGGCCTCACCGGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATg  <  1:117557/251‑1 (MQ=255)                                                                                                                                                                                                                                                       gCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGAGTTCAGCGCCTGAAGAGTCAGAAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGAtt  >  2:271027/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                            TGCCGCATCCGCTTTCAGCAAGCGGTCAGCTTTATTGTCGAGCAATAAATCAGCGAGACGCTTAAGAACCGCGATGGGCTGATCGTCATTCATCGCCACACTCACCAGCATGGCTGCCGTTTCGCC‑GTCCACATCAAAAGCATTTGCCGCACGGCTT‑ACCGCAATCGCGCTACGCAGATTGCC‑TTCGG‑CGCTATCGCTCAGCCAGATACCCTGTC‑CGAGATTCAGCGGTTGTTCATTGATGGCTTT‑GGT‑GACGAAAGTGGCGTCAACTGCCCCCGCCTCTTTCAGACGCGCAGCGTTCAGCGCCTGAAGAGTCAGCAGATCGCTGGCGACGATATCCAGTGTCAGCATTTCGTTGTCGAGCTTCAGCTGCTCACTCTGCTTTTCGCCCATCAGTAATGCGCGAAGTTCTTCTGCTGTTGTTGCTGACTTCAGTTGTTCAGCAACGGAATCATCGCTCAGTACGTGGGTCAGCTGGCGTAGCAGGCCCAGATG  >  NC_000913/2262689‑2263180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑