Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,174,6050TC29.2% 126.3 / 10.4 68G187G (GGA→GGG) ycfTinner membrane protein YcfT
Reads supporting (aligned to +/- strand):  ref base T (38/8);  new base C (0/19);  total (38/30)
Fisher's exact test for biased strand distribution p-value = 1.80e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAAC  >  NC_000913/1174356‑1174854
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                           
cagcagGCTGGTGGAGCGACTACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCTCCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCAtt                                                                                                                                                                                                                                                             <  2:338996/250‑1 (MQ=255)
      gCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCccacc                                                                                                                                                                                                                                                      <  2:65637/251‑1 (MQ=255)
        gggtggAGCGCATACCGAATCGTTGCTCGGATGGAGAAAACAGCTTACTGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACATCCAGG‑CATCGACCGCCAGAAGGGTAAGCAAAGAA‑GCCATCAGCAGATGGCTG‑CGCAAC‑GTCACCTCTTTAACACAGGTCAT‑TATTGTGGCGACAAACCAGGCGCCAG‑GTCTTTAAGAAGGCAAA‑TTTCTGCT‑CCCACTG‑TCCATACCccacctc                                                                                                                                                                                                                                                    <  2:392561/250‑3 (MQ=255)
         ggtggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAC‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACg                                                                                                                                                                                                                                                   >  2:330256/1‑251 (MQ=255)
           tggAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGc                                                                                                                                                                                                                                                 >  1:306649/1‑251 (MQ=255)
                   aTACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑c                                                                                                                                                                                                                                        >  1:267306/1‑251 (MQ=255)
                                       ttGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTc                                                                                                                                                                                                                    >  2:248321/1‑250 (MQ=255)
                                         gATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTcagc                                                                                                                                                                                                                 >  2:481106/1‑251 (MQ=255)
                                          aTAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTcagc                                                                                                                                                                                                                 >  1:28848/1‑250 (MQ=255)
                                           tAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTcagc                                                                                                                                                                                                                 >  2:349939/1‑249 (MQ=255)
                                              aaCAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTac                                                                                                                                                                                                            >  1:104391/1‑251 (MQ=255)
                                                  gCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTACGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTacaaac                                                                                                                                                                                                        >  2:142551/1‑251 (MQ=255)
                                                           ccaccaaAACCGATACCAGCGACAACAGCAGGGAGATAGAAAACACCAAGC‑CCCCGCCCGCAAAAAGGGAAAGAAAAGAA‑CCCATCAGCAGATGGCGG‑CGAAGC‑GGCACCTCTTAAACACAGGTCAT‑TTTTGTGGCGCAAACCCATGCGCCAA‑GGTTGAAAAAAGGCAAA‑TTGCGGAC‑CCCACTG‑TTCATCCCCCCCCACGGCGTGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                               <  1:195755/250‑1 (MQ=255)
                                                            caccacAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑ACCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGAACTAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCAACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAAt                                                                                                                                                                                               >  2:504340/1‑250 (MQ=255)
                                                            caccacAATCGATAACAGCGACAACAGCAGCGAGATAGAAAAAAACCAGG‑CACAGACCGCCAGAACGGTAAACAAAAAA‑ACCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGACAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGGGGGAA‑CGTACTGAA‑CCGACACACTCAGCAGTACAAAACAGGCGAAt                                                                                                                                                                                               >  2:504326/1‑250 (MQ=255)
                                                                acaATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAgagg                                                                                                                                                                                          >  1:91524/1‑248 (MQ=255)
                                                                    ccGATACAAGCGAAAACAGCAGCGAGAGAGTAAACACCCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGC‑CGCAAC‑GGCACCTCTTTACCACGGGTCAG‑TATTGTGGCGCCAACCCAGACGCCAA‑GGCTATAATAAGGAAAA‑TTG‑GGATGCACACTC‑TTCCTCCCCCACCGCGGCGTGGGAA‑CAAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAgc                                                                                                                                                                                      <  1:120787/250‑1 (MQ=255)
                                                                           cAGCGACAAAAGCAGCGAAATAGAAACCACCCAGG‑CCCCGACCGCCAGAACGTTAAGCAAAAAA‑GCCTCCAGCAGATGGCGG‑CGAACC‑GCCCCCTCTTTAACACAGTTCAT‑TATTTTTGCGCCAACCAATGCTCCAA‑GCCTGTAAAAAGGAAAA‑TGGCGAAT‑CACCCTG‑TTCATTCCCCACCGCGGGGTGGAAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGAc                                                                                                                                                                               <  1:109655/251‑1 (MQ=255)
                                                                            aGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACg                                                                                                                                                                              >  1:53387/1‑251 (MQ=255)
                                                                              cGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCACACCACGGCGTGGGAA‑CGCAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGCGGCAGCGCCAGACGGc                                                                                                                                                                            >  1:397776/1‑251 (MQ=255)
                                                                               gACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCAAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGAGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAACCAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                                                                                                                                           >  1:416784/1‑251 (MQ=255)
                                                                               gACAACAGAAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGACAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTCAAAACACGGCGAATAGTGGCAGCGCCAGACGGCt                                                                                                                                                                           >  2:460659/1‑251 (MQ=255)
                                                                               aacaacaGCAGCGAGAAAGTAA‑CCACCAGGGCCCCGACCGCCAGAACGATAAGAAAAGAA‑GCCACCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTGGCCCCAACCCATGCGCCAA‑GGTGGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATCCCCCCCCACGGCGGGGGAA‑CAAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCt                                                                                                                                                                           <  1:147797/250‑1 (MQ=255)
                                                                                   acggaggcGAGATAGAAAACAACCAGG‑CCCCGCCCGCCAGAACGGTAATAAAAGAA‑GCCAGCAGCAGAGGGGGG‑GGCAAC‑GCCCCCTCTTAAACAAAGGTTAT‑TTTTGTGGCGCCAACCCATGCGCAAA‑GGCTGAAAAAAGGCAAT‑TTGCGGAT‑CCCTCTG‑TTAATCCCCCCCCACGGGGGGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTaaaa                                                                                                                                                                       <  2:494762/245‑1 (MQ=255)
                                                                                     agcagcGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAAt                                                                                                                                                                     >  2:343/1‑251 (MQ=255)
                                                                                            agaTAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACAt                                                                                                                                                              >  1:266645/1‑251 (MQ=255)
                                                                                            aaaaagaaaaCAACCAGG‑CACCGCCCCCAAGAACG‑AAAACAAAAAACGCCATAACCAGA‑GGCGGGCGAACC‑GGCACCTCTTAACCACAGGTCTT‑TATTGTTGCGCAAACCCATGCGCAAA‑GGCTGTAA‑AAGGAAAAATGGCGAAT‑CACCCTG‑TCCATCCCCCCCCACGGCGGGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACAt                                                                                                                                                              <  2:266645/244‑1 (MQ=255)
                                                                                             aatagaaaccacccAGG‑CCCCGCCCGCCAGAGCGGAAAGAAAAGAA‑TCCATCAGCAGGTGGCGG‑CGCAAC‑GGCCCCTTTTTAACAAAGTTCAT‑TTTTTTGGCGCCAACCCATGCGCCAG‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CCCCCTT‑TTCTTCCCCCCCCGCGGCGTGGAAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATa                                                                                                                                                             <  1:11422/239‑1 (MQ=255)
                                                                                               aaatAAAAAACCAGG‑CACCGACCGCAAGAACGGTAAGCAAAGAA‑GCCATCGGCAGATGGCGG‑CGCACC‑GGCCCCTCTTAAACAAGGGTCTT‑TATTTTTGCGCCAACCGACGCGCAAA‑GGCTGAAATAAGGAAAA‑TTGCGGAT‑CACACTG‑TTCTTTCCCCCCCGCGGGGTGGGAA‑CAAAATTAT‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATAcc                                                                                                                                                           <  1:187588/248‑1 (MQ=255)
                                                                                                  ataaccaacaggcccccgaccgCCAGAAGGAAAAGCAAAAAC‑GCCAAAAGCAAAGGGGGG‑CGCAAC‑GGAACTCCTTAAACACTGGTTTT‑TATTTTTGCGCCAACCCCTCCGCAAG‑GGCTAAAAAAAGGCAAA‑TGGCGGAT‑CACCCTT‑TTCAT‑CCCCCCCCCCGCGGGGGAAACGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCAc                                                                                                                                                         <  1:232966/236‑1 (MQ=255)
                                                                                                      aaaacgaggcccctgccgCCAGAACGGGAAGCAAAGAA‑GCCCACAGCAGGGGGCG‑‑CGGAACCGGCCCCTCTT‑AACCCAAGTCCTATCTTGTAGCCCAAAACCATGCGCTAA‑GGCTATAAGAAGGCAAA‑TTGCGTCC‑CCCACTC‑ATCATTCCCCCCCACGCTGTGGGAA‑C‑AAATTAAACCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGaaa                                                                                                                                                     <  2:70318/236‑1 (MQ=255)
                                                                                                           cggcccccaacccccAGACCGGTAAGAAAAGAA‑GCCAACAGCAGATGGCGG‑CGCAAC‑GCCCCCTCTTTACCACAGGTAAT‑TATTGTTGCGCCAAACCATGCGCCAG‑GGCTGTAATAAGGCAAA‑GTGCGGAT‑CACACTG‑TTCATTCCCCCCCCCGGCGTGGGAA‑CGAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGAc                                                                                                                                                <  1:241572/238‑1 (MQ=255)
                                                                                                           aGG‑CACCGACCGCCAGAACGGTAAGCAAAGCA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGAc                                                                                                                                                >  1:162206/1‑250 (MQ=255)
                                                                                                                accgaccgCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAAtt                                                                                                                                            >  2:261398/1‑250 (MQ=255)
                                                                                                                 ccgacccCCAAAGCGGGAAGCAAAAAA‑GCCATCAGCAGGGGGGGG‑CAAACC‑GGCCCCTTTTAAACACAGGTAAT‑TTTTGTGGCGCAAACCCAGGCCCAAA‑GGTTGTAAAAAGGAAAA‑TGGCGTAC‑CCCCCTT‑TTCTTCCCCCCCCAGGGCGTGGGAA‑GAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTaa                                                                                                                                          <  2:436393/251‑1 (MQ=255)
                                                                                                                  cgaccgCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAAc                                                                                                                                         >  2:250775/1‑251 (MQ=255)
                                                                                                                   gaccgCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACg                                                                                                                                        >  1:402092/1‑251 (MQ=255)
                                                                                                                               ggTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATAcc                                                                                                                            >  2:442969/1‑251 (MQ=255)
                                                                                                                                gTAAGAAAAGAA‑GCCATCAGCAGAGGGCGG‑CGCAAC‑GGCACTTTGTTAACACAGTACAT‑TATTGTTGCGCCAACCCTGGCGCCAA‑GGCTTTAATAAGGCAAA‑TTGGGGAC‑CACACTG‑TTCATCCCCCCCCAGGGCGGGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACca                                                                                                                           <  2:61866/251‑1 (MQ=255)
                                                                                                                                   aGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACcaca                                                                                                                         >  1:20750/1‑250 (MQ=255)
                                                                                                                                       aagaa‑gCAATCGGCAGAGGGCGC‑CGAACC‑GGCACCTCTTTAACATAGGTATT‑TATTGTGGCGCAAACCCAGGCGCCAA‑TGCTGTAATAGGGCAAA‑TGGCGGA‑‑CCCCCTGGTCCATCCCCCCCCCCGGCGGGGAAA‑CGAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCATACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTg                                                                                                                    <  1:149072/251‑1 (MQ=255)
                                                                                                                                                          gccgg‑caCAAC‑GGCACCTCTTAAACACAGCTCTT‑TTTTGTTGCGCCAACCCATCCGCAAA‑GGGTGAAAAAAGGCAAT‑TTGCGTAT‑CACCCTT‑TTCATACCCCCCCACGGCGGGGGAA‑CGAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGatcatc                                                                                                  <  2:104391/249‑1 (MQ=255)
                                                                                                                                                           gggg‑cgCAAC‑GGCCCCTTTTTAACACAGGTCAT‑TATTGTTGCGCAAAACCAGGCGCCAA‑GGCTGTAATAGGGAAAA‑TTGCGGAT‑CACACTG‑TTCTTCCCCCCCCCCGGCGTGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATcc                                                                                                 <  1:435128/249‑1 (MQ=255)
                                                                                                                                                                 gaaaC‑GGCCCCTTTTAAACACAGTTCAT‑TTTTGTTGCCCAAAACCATGCCCCAA‑GGCTGTAATAAGGAAAA‑GTGGGGAT‑CACCCTG‑TTCATCCCCCCCCCCGGCGTGGAAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGc                                                                                            <  1:395781/249‑1 (MQ=255)
                                                                                                                                                                  cAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCGGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGttc                                                                                            >  1:353817/1‑248 (MQ=255)
                                                                                                                                                                          aCCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGAGATCAACCCCAGCAGGAAcac                                                                                    >  2:474589/1‑249 (MQ=255)
                                                                                                                                                                              cttaaaaacaGGTCAT‑TATTGTGGCGCAAAACCATGCCCCAA‑GGCTGTAAAAAGGAAAA‑TGGCGGAT‑CACCCTG‑TTCATCCCCCCCCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCAc                                                                                  <  1:481106/242‑1 (MQ=255)
                                                                                                                                                                                           aat‑tattGTTGCGC‑AAACCCAGGGCCAAAGGCTTAAATAAGGAAAA‑TTGCGGAT‑CACACTT‑TTCATCCCCCCCCAGGGCGGGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCa                                                                    <  1:168952/249‑1 (MQ=255)
                                                                                                                                                                                                            aaCCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGTACATCCCGCGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCCTTGGAGGc                                                   >  2:110660/1‑251 (MQ=255)
                                                                                                                                                                                                            aaCCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATAATCCCGGGCAGGAACTCACCGGGGGGAACGGGATAAGCGGCATTgggggg                                                   >  2:169304/1‑247 (MQ=255)
                                                                                                                                                                                                               cATGCGCCAG‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTAATTCCCCCCCACGGCGGGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCAtt                                                <  1:343/251‑1 (MQ=255)
                                                                                                                                                                                                                gggccccAA‑GGCTGAAATAAGGCAAA‑TGGCGGAC‑CACACTT‑TTCATCCCCCCCCACGGCGGGGGAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTg                                               <  2:20750/246‑1 (MQ=255)
                                                                                                                                                                                                                   ccccAA‑GGCTGTAAAAAGCCAAT‑TGGCGGAT‑CACACTG‑TCCATCCCCCCCCACGGGGGGGGAA‑CAAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCtt                                            <  1:136534/249‑1 (MQ=255)
                                                                                                                                                                                                                     ccAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTa                                           >  1:81460/1‑250 (MQ=255)
                                                                                                                                                                                                                                aataaGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGGAACGGCATAAGCGGCATTGGAGGCAATGCTTAAATCGCGCTc                                 >  1:39518/1‑250 (MQ=255)
                                                                                                                                                                                                                                 ataagcaaaa‑TTGCGGAT‑CCCACTA‑TCCTGTCCCCACCACGGCGTGGAAA‑CAAAATAAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAg                               <  1:220239/244‑1 (MQ=255)
                                                                                                                                                                                                                                 ataaGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGGGCAGGAACTCACCGGTGGAATCGGCATAAACGGCATTggggggaatggttaaaacccgctcac                               >  2:225992/1‑227 (MQ=255)
                                                                                                                                                                                                                                  acagtaaaa‑TTGTGGAT‑CAC‑CTGGTTCATCCCCCACCACGGCATGGTAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAgg                              <  1:221072/245‑1 (MQ=255)
                                                                                                                                                                                                                                    aGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGGGCAGGAACTCACCCGTGGAATCGGCATAAGCGGCATTGGAGGCATTTGTTAAATCGCGCTCAGGTg                            >  1:261621/1‑251 (MQ=255)
                                                                                                                                                                                                                                       gaaatgggCGGAT‑CACCCTG‑TTCATCCCCCCCCGCGGCGGGGAAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgcc                          <  1:169304/244‑1 (MQ=255)
                                                                                                                                                                                                                                        aaa‑TGGCGGAT‑CACACTG‑TTCATCCCCCACCACGGGGGGGAAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccag                        <  1:214845/251‑1 (MQ=255)
                                                                                                                                                                                                                                              ggcggaacccacTG‑TTCATTCCCCCCCACGGCGTGGAAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTgccagcca                     <  1:248321/241‑1 (MQ=255)
                                                                                                                                                                                                                                                ggAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAACGGCATTGGAGGCCTTGCTTAAATCCCGCTCAGGAGCCAGCCCCTgg                 >  1:378940/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 gAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAAGTGCCAGCCACTggg                >  1:423843/1‑250 (MQ=255)
                                                                                                                                                                                                                                                        acTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCCACCCGCGCCGGGACACACCGGGGGAAACGGCATAAACGGCAATGGAGGCATTGCTTAAATCGCGCTCAGGGGCCAGCCCCTgggttagcg          >  2:426087/1‑244 (MQ=255)
                                                                                                                                                                                                                                                        acTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCCTAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGGGCCAGCCACTGGTTCAgcg          >  1:284899/1‑251 (MQ=255)
                                                                                                                                                                                                                                                          tG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCCAGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGGGCCAGCCCCTAGTTCAgcgc         >  1:73337/1‑250 (MQ=255)
                                                                                                                                                                                                                                                            tttCATCCCCCACCCGGGGGTGGAAA‑CAAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCa        <  1:349939/249‑1 (MQ=255)
                                                                                                                                                                                                                                                               cATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGGGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACCGGGTCAGCGCCCtt     >  1:203136/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                 ttCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCCCAGGTGCCCGCCACTGGGTCAGCGCACTTAAc  >  1:453114/1‑251 (MQ=255)
                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                           
CAGCAGGCTGGTGGAGCGCATACCGAATCGTTGCTCGTATTGATAAAACAGCTTCATGATCACCACAATCGATACCAGCGACAACAGCAGCGAGATAGTAAACAACCAGG‑CACCGACCGCCAGAACGGTAAGCAAAGAA‑GCCATCAGCAGATGGCGG‑CGCAAC‑GGCACCTCTTTAACACAGGTCAT‑TATTGTTGCGCCAAACCATGCGCCAA‑GGCTGTAATAAGGCAAA‑TTGCGGAT‑CACACTG‑TTCATTCCCCACCACGGCGTGGGAA‑CGAAATTAA‑CCGCCACACTCAGCAGTACAAACAAGGCGAATAGTGGCAGCGCCAGACGGCTAAAAATTTTACATACCACGAAATAGACAATTAACGCATACAGATACCACAAGCTGGTGCTGGCGGTGATCATCCCGTGCAGGAACTCACCGGTGGAATCGGCATAAGCGGCATTGGAGGCATTGCTTAAATCGCGCTCAGGTGCCAGCCACTGGTTCAGCGCACTTAAC  >  NC_000913/1174356‑1174854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑