Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,867,714	0	T	A	23.2%	128.0 / 10.6	56	I269I (ATT→ATA)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base T (32/11);  new base A (0/13);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 1.32e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCA  >  NC_000913/1867465‑1867950                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               aTTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTa                                                                                                                                                                                                                                               <  2:339927/251‑1 (MQ=255)    tggtggCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTaa                                                                                                                                                                                                                                              >  2:277404/1‑249 (MQ=255)        ggCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGtgct                                                                                                                                                                                                                                        <  1:465230/251‑1 (MQ=255)              atCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCAGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATc                                                                                                                                                                                                                                  <  1:171133/250‑1 (MQ=255)                cACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATccc                                                                                                                                                                                                                                <  1:460816/250‑1 (MQ=255)                      gTTCCGGGTAGTGAAGTTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCTGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑AGGAATTCGTTGCGATGTTTAAA‑GCACCGATTAAATTGCTGC‑ATACCTT‑GTTa                                                                                                                                                                                                                         >  2:219530/1‑250 (MQ=255)                       ttCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAAc                                                                                                                                                                                                                       >  2:375684/1‑251 (MQ=255)                                      gTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTcaggcagg                                                                                                                                                                                                        >  1:210265/1‑251 (MQ=255)                                              gtaaaTTCTGAAACAAATTGCTACCGCAAAAACGGACCCCGGCTATGAGAAAAACCAGGACATCCCCGCGCTGGTTGGAAAA‑GTCGTTATTCGTAACC‑TCGAACACTCCGCGCAGAATGACCGGGA‑CGCCTAGGGTTATTCGGG‑TGCGCTGTGCCGCGCAAATCAGGGG‑ATCA‑TGGATTTCGTTGAGATTTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑TTTAACCGCCACTCAGGAAGGTAACTACa                                                                                                                                                                                                <  1:457648/248‑1 (MQ=255)                                                     cTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGAc                                                                                                                                                                                         >  1:2510/1‑251 (MQ=255)                                                      ggaaacaaATTGCTATCGCAAAAACGGACCCGGGCGTTGAGAAAAACCAGGCCATCCCCGCGTGGGTGGGGAAA‑GTCGATATTCGTAAAC‑TCGACCCCGACGCGCAGAAGGACCCGGA‑CGCCTACGTCTATTCGGG‑TGCGCTGTGCCGCGCCAA‑CAGGGGGATAA‑TGGAAGTCGTTGGGATGTTAAAA‑GCACCGATAAAAGTGCTGC‑ACCCTTT‑TTTAACCGCCACTCAGGAAGGTAACTACAACGGGACg                                                                                                                                                                                        <  2:430842/248‑1 (MQ=255)                                                       gCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACgg                                                                                                                                                                                       >  1:343335/1‑251 (MQ=255)                                                       gCAAAAAATTGCTACCGCCAAAACGGACCCCGGCGATGAGAACACCCAGGACATCTCCGCGCTGGTTGGAAAA‑GTCGATATTCGTAAAC‑TCGACCACTACGCGCAAAATGACCCGGA‑CGCCTACGGCTATTCCGG‑GGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCCCCAATTAAGGTGCTGC‑ACCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACg                                                                                                                                                                                        <  1:30707/250‑1 (MQ=255)                                                         aacaaATTGCTATCGCCAAAACGGAACACGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGa                                                                                                                                                                                      >  2:461750/1‑250 (MQ=255)                                                          acaaATTGCGACCGCCAAAAGGGAACCGGGGGATGAAAACAACCAGGACTCCCCCGCG‑TGGGTGGGAAAAGTAGATATTCGAAACC‑CCGACCACTCCGCCAAGAATGCCCCGGC‑CCCCT‑CGGGTTTTCCGGGTGGGCTGGGCGGCCCAAAGCGGGGG‑ATCA‑GGGAGTTGGTGGAG‑TGTTTAAAAGCACCGATAAAAGGGCTGC‑ACCCTTT‑GTAAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAg                                                                                                                                                                                    <  2:131953/251‑1 (MQ=255)                                                                tGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTCAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTAtct                                                                                                                                                                              >  1:146264/1‑251 (MQ=255)                                                                   tacagCCAAAACGGACCCCGGGGAGGAGAACAACCAGGACACCCCCGCGCGGGTGGGGAAA‑GTCGATATTCGTAAAC‑CCGAACACTCCGCGCAGAATGTCCCGGC‑CGCCGAGGGCTTTTCGGG‑TGCGCTGGGCCGCGCCAATCAGGGG‑‑TCAATGGAATTCGTTGAGATGTTAAAA‑GCACCAAAAAAAGGGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTAtctc                                                                                                                                                                             <  1:277404/245‑1 (MQ=255)                                                                    agccccaaacgggAACCCGGCGAGGAGAACAACCAGGACACCCCCGCGGTGGTTGGGAAA‑GTCGTTATTCGAAACC‑TCGACCCCTACGCGAAGAATGCCCCGGC‑CGCCAACGGCTTTTCGGG‑GGCGCGGTGCCGCGCAAAACGGGGG‑ATCA‑GGAAATTCGTGGAGATTTTAAAA‑CCACGGATTAAAGTGCTGC‑ACCCGTT‑GTTACCCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTcc                                                                                                                                                                            <  2:127896/238‑1 (MQ=255)                                                                        ccAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTg                                                                                                                                                                      >  1:61494/1‑251 (MQ=255)                                                                          caaaCGGAACCCGGCGTTGAGAACAACCAGGACATCTCCGCGCTTGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑TTCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTAAAACGGGACGGCAGGTATCTCCGCCCTGcc                                                                                                                                                                    >  2:300566/2‑251 (MQ=255)                                                                             acagAACCGGGCGAGGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATAACCCAGA‑CGCCTACGTCTATGCCGG‑TGCGCTGGGCCGCGCCAATCAGGGG‑ATCA‑TTGAATTCGTTGAGATGTTTAAA‑GCACCGGTTCAAGGGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGAAAGGTAACAACAACGGGACGGAAGGTATCTCAGCCATGcctgt                                                                                                                                                                 >  2:8282/4‑248 (MQ=255)                                                                              cGGACCCCGGCGATGGAAACACCCAGGACATCTCCGCGTTGTTGGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAAGGCCCGGGA‑CGCCTCCGGCTATTCCGG‑TGCGCTGTGCCGCGCAAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTAAAA‑CCCCCGATAAAAGTGTGGC‑ATCCTTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTc                                                                                                                                                                <  1:180876/251‑1 (MQ=255)                                                                                 aaCCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAAc                                                                                                                                                             >  1:481800/1‑251 (MQ=255)                                                                                          aTGAGAACAACCAGACCATCCCCGCGCTGGTGGGGAAA‑GTCGTTATTCGTAACC‑TCGACCCCTCCGCGCAGAATGCCCCGGC‑CGCCTCCGGCTTTTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGATTTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTAAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGattatt                                                                                                                                                    <  1:513451/251‑1 (MQ=255)                                                                                               aacaacCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGc                                                                                                                                               >  1:287028/1‑251 (MQ=255)                                                                                                acaacCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGCACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGc                                                                                                                                               >  1:385465/1‑250 (MQ=255)                                                                                                          cATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACg                                                                                                                                    >  1:436579/1‑251 (MQ=255)                                                                                                              tcCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTc                                                                                                                                >  2:227916/1‑251 (MQ=255)                                                                                                                   gcTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAAt                                                                                                                           >  1:438019/1‑251 (MQ=255)                                                                                                                      ggTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATggg                                                                                                                        >  1:35635/1‑251 (MQ=255)                                                                                                                       gTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGt                                                                                                                       >  1:111849/1‑251 (MQ=255)                                                                                                                           ggAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGAAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑CGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTGCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCAcc                                                                                                                   >  2:268621/1‑250 (MQ=255)                                                                                                                            gAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑AACA‑TGGAATTCGTTGAGAAGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGGTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACtt                                                                                                                  >  2:513691/1‑251 (MQ=255)                                                                                                                             aaa‑GTCGATATTC‑TAAACCTCGAACCCCACGCGCAGAATGCCCCGGA‑CGCTTACGTCTATTCGGG‑TGCGCGGTGCCGCGCAAATAGGGGG‑ATCA‑TGGAATTCGTTGAGATGTTAAAA‑GCACCGATAAAAGTGCTCA‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACttt                                                                                                                 <  1:239832/251‑1 (MQ=255)                                                                                                                                    gATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCCCACTCGAACGAGTCCGCATGGGTCCCTTTCCGta                                                                                                            >  2:327878/1‑250 (MQ=255)                                                                                                                                    gATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCCGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGta                                                                                                            >  1:373793/1‑250 (MQ=255)                                                                                                                                        ttCGTAAAC‑TCAAACCCTCCGCACAGAATGCCCCGGA‑CCCCTCCGGCTATTCCGG‑GGCGCTGTGCCGCGCCATTCGGGGG‑ATCA‑TGGATTTCGTTGAGATGTTTAAA‑GCCCCGATAAAGGTGCTGC‑ATCCCTT‑TTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGtaataa                                                                                                        <  1:389696/250‑1 (MQ=255)                                                                                                                                         tCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTAGCACACTCGAACGAGTCCGACTGGGTCACTTTCCGTAATAAc                                                                                                       >  2:304521/1‑250 (MQ=255)                                                                                                                                                          cccgcgCAGAAGGACC‑GGAGCGCCTAGGGCTATTCCGG‑GGCGCTGGCCCGCGCAAATCAGGGG‑AAAA‑GGGATTTCGTTGAGATTTTTAAA‑GCACCAATTAAAGTG‑TGCCATCCCTT‑TTAAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCtt                                                                                      <  1:253665/249‑1 (MQ=255)                                                                                                                                                           aCGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTc                                                                                     >  2:96186/1‑251 (MQ=255)                                                                                                                                                                   aaTGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCg                                                                             >  1:423470/1‑251 (MQ=255)                                                                                                                                                                      gACCGGGA‑CGCCTACGGCTATTCCGG‑GGCGTGGGGCCGCGCCAATCAGGGA‑ATCA‑TGAAATTCGTGTAAATTTTAAAA‑GCCCCGATAAAAGTGCTCC‑CTCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAGGCCTTCCTCGATCgtgt                                                                          <  1:105490/251‑1 (MQ=255)                                                                                                                                                                       aCCCGGA‑CGACTACGGCTAGTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGATCAACGGGACTATTCTCGCACACTCGAACGAGACCGAATGGGTCACTTTACGTAATAACACAAACAACGAAGCCTTCCTCGATCGTGtt                                                                         >  2:447859/1‑251 (MQ=255)                                                                                                                                                                             gccgcatacgttttttCCGG‑TGCGCTGTGCCGCCCCGAGCGGGGG‑ATAT‑TGGAATTGGTTGAGATTTTAAAA‑CCACCGATTAAAGTGTTGC‑AACCCTT‑TTAAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACAt                                                                    <  1:21143/237‑1 (MQ=255)                                                                                                                                                                                    ccggattttCCGG‑TGCGCTGTGCCGACACAAACGGGGG‑ATCA‑TGAAATGGGTTGAGATTTTAAAA‑GCACCGATAAAAGTCCTGC‑ATCCTTT‑CTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGaa                                                              <  2:502794/243‑1 (MQ=255)                                                                                                                                                                                                         gcgccccgcaattcggggaaTCA‑GGGAATGCGTTGAGAGCTTAAAA‑GCGCCGATAAAAGTGCT‑C‑AACCCTTTCTAAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCa                                          <  1:311496/232‑1 (MQ=255)                                                                                                                                                                                                                ccAATCAGGGG‑ATCA‑TGAAATTCGTGGAGATGTTAAAA‑GCACCGATAAAAGTGCTGC‑ATCCCTT‑TTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGaa                                  <  1:44832/251‑1 (MQ=255)                                                                                                                                                                                                                 cAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGa                                   >  1:60235/1‑249 (MQ=255)                                                                                                                                                                                                                   aacAGGGG‑ATCG‑TGGAATTCGTTTAGATGTTAAAA‑GCCCCGATAAAAGTGCTGA‑TTCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGGAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAagag                               <  1:455044/249‑1 (MQ=255)                                                                                                                                                                                                                    tCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                 >  1:385544/1‑248 (MQ=255)                                                                                                                                                                                                                    tCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                 >  1:419074/1‑248 (MQ=255)                                                                                                                                                                                                                     cAGGGG‑AACA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAag                                 >  1:506094/1‑247 (MQ=255)                                                                                                                                                                                                                      ggggg‑ACCA‑TGGAATTCGTTGAGATTTTAAAA‑CCACCGATAAAAGTGCTCC‑ACCCCTT‑TTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATc                            <  1:375684/250‑1 (MQ=255)                                                                                                                                                                                                                        ggg‑ATCA‑TGGAATTCGTTGAGATGTTAAAA‑GCCCCGATAAAAGTGCTGC‑ACCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCaa                          <  1:236916/251‑1 (MQ=255)                                                                                                                                                                                                                                 tGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCGCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTAc                   >  1:501871/1‑251 (MQ=255)                                                                                                                                                                                                                                                    taaaaccACCAATTAAAGTGCTGC‑ACCCTTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATca  <  1:513298/244‑1 (MQ=255)                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ATTTGGTGGCGATATCACTAAGTTCCGGGTAGTGAAGGTCTGGCCGTCAATTCTGCAACAAATTGCTATCGCCAAAACGGAACCCGGCGATGAGAACAACCAGGACATCTCCGCGCTGGTTGGGAAA‑GTCGATATTCGTAAAC‑TCGAACACTACGCGCAGAATGACCCGGA‑CGCCTACGGCTATTCCGG‑TGCGCTGTGCCGCGCCAATCAGGGG‑ATCA‑TGGAATTCGTTGAGATGTTTAAA‑GCACCGATTAAAGTGCTGC‑ATCCCTT‑GTTAACCGCCACTCAGGAAGGTAACTACAACGGGACGGAAGGTATCTCCGCCCTGCCGTTCAACGGGATTATTCTCGCACACTCGAACGAGTCCGAATGGGTCACTTTCCGTAATAACAAAAACAACGAAGCCTTCCTCGATCGTGTTTACATCGTGAAGGTGCCGTATTGCTTGCGCATTTCCGAAGAGATCAAAATCTACGAGAAATTGCTTAATCA  >  NC_000913/1867465‑1867950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑