mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 155,463 | A→T | *247K (TAA→AAA) | yadV ← | putative fimbrial chaperone YadV |
| MC JC | 244,301 | Δ9,446 bp | yafJ–yafP | yafJ, dpaA, yafQ, dinJ, yafL, rayT, dinB, yafN, yafO, yafP | |
| RA | 254,249 | A→T | intergenic (+1088/+10) | yafP → / ← pepD | putative N‑acetyltransferase YafP/peptidase D |
| MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
| RA | 389,601 | C→T | D43N (GAC→AAC) | hemB ← | porphobilinogen synthase |
| RA | 389,867 | T→C | intergenic (‑140/‑384) | hemB ← / → yaiT | porphobilinogen synthase/putative autotransporter YaiT |
| MC JC | 390,251 | Δ14,565 bp | [yaiT]–[dgcC] | 16 genes [yaiT], ytiB, insF2, insE2, yaiT, iprA, ampH, sbmA, yaiW, yaiY, yaiZ, ddlA, iraP, phoA, psiF, [dgcC] |
|
| MC JC | 519,143 | Δ14,682 bp | [ybbO]–[allZ] | [ybbO], [tesA], ybbA, ybbP, rhsD, ybbC, ylbG, selU, allS, allA, [allR], [allZ] | |
| RA | 535,337 | T→C | A474A (GCT→GCC) | gcl → | glyoxylate carboligase |
| RA | 536,173 | T→C | L155P (CTC→CCC) | hyi → | hydroxypyruvate isomerase |
| RA | 536,492 | A→G | intergenic (+6/‑94) | hyi → / → glxR | hydroxypyruvate isomerase/tartronate semialdehyde reductase 2 |
| RA | 536,549 | T→C | intergenic (+63/‑37) | hyi → / → glxR | hydroxypyruvate isomerase/tartronate semialdehyde reductase 2 |
| MC JC | 536,617 | Δ14,712 bp | [glxR]–[ybcF] | [glxR], ybbW, allB, ybbY, glxK, allE, allC, allD, fdrA, ylbE, ylbF, [ybcF] | |
| RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
| MC JC | 565,054 | 44181 bp→TGA | [intD]–insL2 | 52 genes [intD], renD, insE3, insF3, renD, emrE, ylcJ, ybcK, ybcL, ybcM, ylcH, ybcN, ninE, ybcO, rusA, ylcG, quuD, nmpC, insH2, nmpC, essD, rrrD, rzpD, rzoD, borD, ybcV, ybcW, ylcI, nohD, tfaD, tfaX, appY, ompT, envY, ybcH, nfrA, nfrB, cusS, cusR, cusC, cusF, cusB, cusA, pheP, ybdG, nfsB, ybdF, ybdJ, ybdK, sokE, hokE, insL2 |
|
| RA | 670,635 | A→T | L324Q (CTG→CAG) | holA ← | DNA polymerase III subunit delta |
| RA | 673,386 | C→A | P466P (CCG→CCT) | leuS ← | leucine‑‑tRNA ligase |
| RA | 1,048,596 | G→A | G32G (GGC→GGT) | gfcC ← | capsule biosynthesis GfcC family protein |
| MC JC | 1,048,805 | Δ48,085 bp | [gfcB]–ycdU | 45 genes [gfcB], gfcA, insA4, insB4, cspH, cspG, ymcF, ymcE, gnsA, yccM, torS, torT, torR, torC, torA, torD, cbpM, cbpA, yccE, agp, yccJ, wrbA, ymdF, rutG, rutF, rutE, rutD, rutC, rutB, rutA, rutR, putA, ymdG, putP, efeO, efeB, phoH, pgaD, pgaC, pgaB, pgaA, dgcT, insF4, insE4, ycdU |
|
| JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
| RA | 1,196,861 | A→G | intergenic (+79/+6) | C0293 → / ← ymfD | small regulatory RNA C0293/putative SAM‑dependent methyltransferase YmfD |
| MC JC | 1,196,867 | Δ27,041 bp | [ymfD]–ymgJ | 37 genes [ymfD], ymfE, lit, intE, xisE, ymfH, ymfI, ymfJ, ymfK, ymfT, ymfL, ymfM, ymfN, ymfR, beeE, ymfQ, ycfK, tfaP, tfaE, pinE, mcrA, iraM, ymgK, ymgL, ycgX, bluR, bluF, ycgZ, ymgA, ariR, ymgC, pdeG, ymgF, ymgD, ymgG, ymgI, ymgJ |
|
| MC JC | 1,299,499 | Δ1,199 bp | insH21 | insH21 | |
| RA | 1,301,917 | C→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,301,919 | G→T | Q246Y (CAG→TAT) | oppA → | oligopeptide ABC transporter periplasmic binding protein |
| RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
| RA | 1,628,332 | T→C | intergenic (+69/‑20) | ydfG → / → rspR | 3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR |
| MC JC | 1,628,340 | Δ10,127 bp | rspR–[cspI] | 17 genes rspR, ydfZ, ydfI, ydfJ, ynfT, ydfK, pinQ, tfaQ, stfQ, nohA, ynfO, ydfO, gnsB, ynfN, ynfR, ynfQ, [cspI] |
|
| RA | 1,638,571 | G→A | H33Y (CAT→TAT) | cspI ← | cold shock‑like protein CspI |
| RA | 1,640,045 | +C | coding (13/534 nt) | rrrQ ← | putative lysozyme |
| MC JC | 1,640,057 | Δ7,591 bp | [rrrQ]–[dicC] | 18 genes [rrrQ], [ydfR], essQ, ynfU, cspB, cspF, ynfS, ydfT, ydfU, rem, hokD, relE, relB, ydfV, flxA, ydfW, ydfX, [dicC] |
|
| RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| JC JC | 2,016,437 | Δ2 bp :: IS186 (–) +8 bp :: Δ1 bp | coding (570‑577/687 nt) | fliH → | flagellar biosynthesis protein FliH |
| RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
| RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| RA | 2,867,454 | 2 bp→AA | coding (97‑98/993 nt) | rpoS ← | RNA polymerase sigma factor RpoS |
| RA | 2,926,772 | C→A | G155G (GGC→GGA) | ppnN → | nucleotide 5'‑monophosphate nucleosidase |
| RA | 2,985,726 | A→G | intergenic (‑134/‑121) | yqeF ← / → yqeG | putative acyltransferase/putative transporter YqeG |
| RA | 2,985,816 | T→C | intergenic (‑224/‑31) | yqeF ← / → yqeG | putative acyltransferase/putative transporter YqeG |
| MC JC | 2,985,842 | Δ47,769 bp | yqeG–[idi] | 39 genes yqeG, yqeH, yqeI, yqeJ, yqeL, yqeK, ygeG, ygeH, ygeI, insD4, insC4, ygeQ, glyU, actS, xdhA, xdhB, xdhC, ygeV, ygeW, ygeX, ygeY, hyuA, yqeA, yqeB, yqeC, mocA, ygfK, ssnA, ygfM, xdhD, xanQ, guaD, ghxQ, ygfS, ygfT, uacT, yqfG, yqfH, [idi] |
|
| MC JC | 3,110,591 | Δ24,234 bp | [yghD]–yghT | 20 genes [yghD], yghE, yqhJ, yghF, yghG, pppA, yghJ, glcA, glcB, glcG, glcF, glcE, glcD, glcC, yghO, insH9, yghQ, yghR, yghS, yghT |
|
| RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
| RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
| JC JC | 3,707,484 | IS3 (+) +3 bp | coding (220‑222/1608 nt) | dppA ← | dipeptide ABC transporter periplasmic binding protein |
| JC JC | 3,720,136 | IS3 (–) +3 bp :: +TCA | coding (88‑90/213 nt) | cspA → | cold shock protein CspA |
| JC JC | 3,890,411 | IS1 (–) +9 bp | coding (176‑184/1248 nt) | tnaB → | tryptophan:H(+) symporter TnaB |
| MC JC | 4,060,444 | Δ19,761 bp | yihL–yiiF | 19 genes yihL, yihM, yihN, ompL, yihO, yihP, yihQ, yihR, yihS, yihT, yihU, yihV, csqR, yihX, yihY, dtd, fabY, yiiE, yiiF |
|
| MC JC | 4,086,996 | Δ13,531 bp | yiiG–rhaT | yiiG, frvR, frvX, frvB, frvA, rhaM, rhaD, rhaA, rhaB, rhaS, rhaR, rhaT | |
| RA | 4,139,288 | C→T | D141N (GAC→AAC) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| RA | 4,139,511 | Δ1 bp | coding (198/663 nt) | fsaB ← | fructose‑6‑phosphate aldolase 2 |
| MC JC | 4,139,771 | 10483 bp→TG | [ptsA]–[eptC] | [ptsA], frwC, frwB, pflD, pflC, frwD, yijO, [eptC] | |
| RA | 4,235,651 | T→C | intergenic (+244/‑255) | pgi → / → yjbE | glucose‑6‑phosphate isomerase/uncharacterized protein YjbE |
| RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| MC JC | 4,299,522 | Δ12,535 bp | mdtP–alsB | mdtP, mdtO, mdtN, ytcA, yjcS, alsK, alsE, alsC, alsA, alsB | |
| RA | 4,312,070 | Δ1 bp | intergenic (‑28/+31) | alsB ← / ← alsR | D‑allose ABC transporter periplasmic binding protein/DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,151 | G→C | Q281E (CAA→GAA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,215 | A→G | T259T (ACT→ACC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,219 | A→G | L258P (CTA→CCA) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,289 | A→G | Y235H (TAT→CAT) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,312,498 | C→G | R165P (CGC→CCC) | alsR ← | DNA‑binding transcriptional repressor AlsR |
| RA | 4,313,027 | T→G | intergenic (‑36/‑323) | alsR ← / → rpiB | DNA‑binding transcriptional repressor AlsR/allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B |
| MC JC | 4,313,864 | Δ16,405 bp | yjdP–rdcB | 18 genes yjdP, phnP, phnO, phnN, phnM, phnL, phnK, phnJ, phnI, phnH, phnG, phnF, phnD, phnC, yjdN, yjdM, rdcA, rdcB |
|
| RA | 4,558,907 | T→A | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 238194 | 238635 | 442 | 5 [4] | [4] 6 | [ykfM] | [ykfM] |
| * | * | ÷ | NC_000913 | 263076 | 315228 | 52153 | 30 [0] | [0] 20 | ykfI–ykgR | ykfI,yafW,ykfH,ykfG,yafX,ykfF,ykfB,yafY,yafZ,ykfA,perR,insN,insI1,insN,eyeA,ykfC,insH1,mmuP,mmuM,afuC,insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR |
| * | * | ÷ | NC_000913 | 315400–316474 | 388643 | 72170–73244 | 5 [4] | [0] 30 | [insE1]–[tauD] | [insE1],insF1,rclC,rclB,rclA,rclR,ykgE,ykgF,ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,lacY,lacZ,lacI,mhpR,mhpA,mhpB,mhpC,mhpD,mhpF,mhpE,mhpT,yaiL,frmB,frmA,frmR,yaiO,yaiX,insC1,insD1,yaiX,yaiP,ykiC,yaiS,tauA,tauB,tauC,[tauD] |
| * | * | ÷ | NC_000913 | 1103666 | 1103783 | 118 | 6 [4] | [3] 5 | csgD/csgB | DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit |
| * | * | ÷ | NC_000913 | 1638632 | 1638812 | 181 | 5 [4] | [4] 6 | [cspI] | [cspI] |
| * | * | ÷ | NC_000913 | 1792128 | 1792259 | 132 | 5 [4] | [4] 5 | rflP/nlpC | anti‑FlhDC factor RflP/NlpC/P60 family lipoprotein NlpC |
| * | * | ÷ | NC_000913 | 1978495 | 1979270 | 776 | 42 [0] | [1] 26 | insB5–insA5 | insB5,insA5 |
| * | * | ÷ | NC_000913 | 2103982 | 2104122 | 141 | 5 [4] | [4] 5 | wbbK | putative glycosyltransferase WbbK |
| * | * | ÷ | NC_000913 | 2229063 | 2229325 | 263 | 7 [3] | [4] 5 | [yohP] | [yohP] |
| * | * | ÷ | NC_000913 | 2406788 | 2407179 | 392 | 71 [1] | [0] 46 | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
| * | * | ÷ | NC_000913 | 2470113 | 2470327 | 215 | 6 [4] | [4] 5 | yfdI | serotype‑specific glucosyl transferase YfdI |
| * | * | ÷ | NC_000913 | 2877915 | 2878267 | 353 | 5 [4] | [4] 5 | iap/cas2 | alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2 |
| * | * | ÷ | NC_000913 | 2903692 | 2903867 | 176 | 5 [4] | [4] 5 | ygcE/queE | putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE |
| * | * | ÷ | NC_000913 | 3423862–3424231 | 3424410–3424238 | 8–549 | 5 [4] | [4] 5 | [rrlD] | [rrlD] |
| * | * | ÷ | NC_000913 | 3597850 | 3597932 | 83 | 5 [4] | [4] 5 | livK/panZ | L‑leucine/L‑phenylalanine ABC transporter periplasmic binding protein/PanD maturation factor |
| * | * | ÷ | NC_000913 | 3798981 | 3799305 | 325 | 5 [4] | [3] 5 | waaU | putative ADP‑heptose:LPS heptosyltransferase 4 |
| * | * | ÷ | NC_000913 | 3804470 | 3805087 | 618 | 5 [3] | [4] 5 | waaS | lipopolysaccharide core biosynthesis protein WaaS |
| * | * | ÷ | NC_000913 | 4235817 | 4252280 | 16464 | 5 [3] | [0] 6 | yjbE–malM | yjbE,yjbF,yjbG,yjbH,yjbT,psiE,xylE,malH,malG,malF,malE,malK,lamB,malM |
| * | * | ÷ | NC_000913 | 4475987 | 4476273 | 287 | 5 [2] | [4] 5 | yjgL | protein YjgL |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 263075 | 0 (0.000) | 30 (0.570) | 30/482 | 0.5 | 100% | intergenic (+129/+253) | thrW/ykfI | tRNA‑Thr/toxin of the YkfI‑YafW toxin‑antitoxin system |
| ? | NC_000913 | 388644 = | 0 (0.000) | coding (850/852 nt) | tauD | taurine dioxygenase | |||||
| * | ? | NC_000913 | = 1397238 | NA (NA) | 42 (0.800) | 39/486 | 0.3 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
| ? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
| * | ? | NC_000913 | 1978503 = | NA (NA) | 70 (1.330) | 63/484 | 0.0 | 100% | noncoding (768/768 nt) | IS1 | repeat region |
| ? | NC_000913 | = 2406787 | 0 (0.000) | intergenic (‑146/‑774) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA | |||||
| * | ? | NC_000913 | 1979271 = | 1 (0.020) | 25 (0.470) | 25/486 | 0.7 | 96.2% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
| ? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
| * | ? | NC_000913 | 2407180 = | 0 (0.000) | 46 (0.870) | 45/484 | 0.2 | 100% | intergenic (‑539/‑381) | lrhA/alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
| ? | NC_000913 | 3583428 = | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region | |||||