Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,139,511 Δ1 bp coding (198/663 nt) fsaB ← fructose‑6‑phosphate aldolase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,139,5110C.97.5% 172.4 / ‑1.3 40coding (198/663 nt)fsaBfructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base . (19/20);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.27e-01

ACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTT  >  NC_000913/4139272‑4139739
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
aCCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCgg                                                                                                                                                                                                                           >  1:339826/1‑251 (MQ=255)
   gTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTc                                                                                                                                                                                                                        >  1:425019/1‑251 (MQ=255)
             cgtttcCGCCCTGGGCATCTACGCGGTTAAAATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCGAGTGTAGTAATACCCTCTTTTTTCAGTCTTTTAATTGCTGCCAGAACTTCGGACGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCCCATGGTCTg                                                                                                                                                                                                                >  2:444166/5‑249 (MQ=255)
                      ccTGGGCATCTACGCGGGTAACATGCGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACATCAGTGGCAAGTGTAGTAATACCCTCTTTTTGCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCTCCGGGATTGTCACCTCAATACCCGGAAGAGCGTCGCGCAGGCGCGTCGCTTCATCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGaa                                                                                                                                                                                                     <  1:445137/251‑1 (MQ=255)
                         gggCTTCTACGCGGTTAACATACGGAGCAACGTATTTGGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAAt                                                                                                                                                                                                    <  2:380476/249‑1 (MQ=255)
                         gggCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCCTGGTCTGAGCCAACAGAAt                                                                                                                                                                                                    >  2:65090/1‑249 (MQ=255)
                         gggCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCA‑GCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAAt                                                                                                                                                                                                    <  1:65090/249‑1 (MQ=255)
                         cggCATCTACGCCGTTAACATACGGAGCACCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAATCAGAAt                                                                                                                                                                                                    <  2:380479/248‑1 (MQ=255)
                             aTCTAAGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGATAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCtc                                                                                                                                                                                              >  2:459669/1‑251 (MQ=255)
                                      gTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAAt                                                                                                                                                                                     <  1:459669/251‑1 (MQ=255)
                                        tAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATc                                                                                                                                                                                    <  2:418568/250‑1 (MQ=255)
                                               cGGAGCACCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCa                                                                                                                                                                                       >  2:302509/1‑240 (MQ=255)
                                               cGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCa                                                                                                                                                                                       <  1:302509/240‑1 (MQ=255)
                                                              tGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCCGACGCGGCAGCAc                                                                                                                                                             >  2:471634/1‑251 (MQ=255)
                                                              tGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAg                                                                                                                                                                         >  2:403848/1‑239 (MQ=255)
                                                              tGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCGTTTTGCAg                                                                                                                                                                         <  1:403848/239‑1 (MQ=255)
                                                               gCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACt                                                                                                                                                            >  2:157211/1‑251 (MQ=255)
                                                                 cccTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTc                                                                                                                                                          <  2:21890/251‑1 (MQ=255)
                                                                              gCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTcc                                                                                                                                             <  1:254707/251‑1 (MQ=255)
                                                                                    aaCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTg                                                                                                                                      <  2:339826/251‑1 (MQ=255)
                                                                                     aCCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTgg                                                                                                                                     >  2:410465/1‑251 (MQ=255)
                                                                                       ccTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCa                                                                                                                                   <  1:191452/251‑1 (MQ=255)
                                                                                                              agTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTATGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGCCTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGt                                                                                                            >  2:445935/1‑251 (MQ=255)
                                                                                                                  ccAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCAcc                                                                                                        >  2:375843/1‑251 (MQ=255)
                                                                                                                              aTACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTAGGAAGTCATCGGGATTTTCACCACAATACCCGGAATCGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATCTGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATgggg                                                                                            >  1:219148/1‑251 (MQ=255)
                                                                                                                                             aGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGCATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCTCTTTTTGCAGACGCGGCAGCCCTTCCCATAGGGACTCC‑TTGCTGGCAGCGATAATGACCGGGTTAGTGGTCACCCCGGCAATTGGGAATATGCGTGcc                                                                                >  2:268031/1‑248 (MQ=255)
                                                                                                                                                  tttAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTc                                                                        >  1:328274/1‑251 (MQ=255)
                                                                                                                                                          ctgcCAGACCTTCGGAAGTCAACGGGCTTTTCACCACAATACCCGGCATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAAAAGAATGCCCTCATCACCAATCGCTTTTTGCGGCCGCGGCAGCACTTCCAATATGG‑CTCCCTTACTGGCAGCGATAATGCCCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGCCGTTCGAATTCTg                                                                >  2:211161/1‑251 (MQ=255)
                                                                                                                                                              cAGAACTTCGGAAGTCACCGGGAGTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCCTCTTCCACCCTCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCACACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCTGCAGCACTTCCCATATGGACGCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGGTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGAc                                                            <  1:299440/251‑1 (MQ=255)
                                                                                                                                                               aGACCTTCGGAAGTCACCGGTATTTTCACCCCAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGGCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACg                                                           <  2:321110/251‑1 (MQ=255)
                                                                                                                                                                gACCTTCGGCAGTCACCGGGCTTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGt                                                          >  2:279812/1‑251 (MQ=255)
                                                                                                                                                                     tCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTCCGACTTCTGCGACGTTAg                                                       <  2:425019/249‑1 (MQ=255)
                                                                                                                                                                      cGGAAGTCACCGGGATTTTCACCCCAATACCCGGAATAGCGTCGCGCGGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGACCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCCCTTCCCATATGGACTCA‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGACAGACGTTCGACTTCTGCGACGTTAGCgg                                                    >  2:424954/1‑251 (MQ=255)
                                                                                                                                                                          aGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTc                                                <  2:53027/251‑1 (MQ=255)
                                                                                                                                                                             cACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCa                                              <  1:157211/250‑1 (MQ=255)
                                                                                                                                                                                cGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATa                                          <  1:410465/251‑1 (MQ=255)
                                                                                                                                                                                 gggATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTGGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATAc                                         <  1:253264/251‑1 (MQ=255)
                                                                                                                                                                                  ggATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATAc                                         >  2:5963/1‑250 (MQ=255)
                                                                                                                                                                                               caTACCCGTAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTCCCATGATGtt                           <  2:261064/250‑1 (MQ=255)
                                                                                                                                                                                                                        cgcttcgcttCTTCCACCATCCC‑TGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCGGCGACGTTAGCGGTGTCCAGAGACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGtt  <  1:471634/251‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                     
ACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTCATGGTCTGAGCAAACAGAATGCCCTCATCACCAATCGCTTTTTGCAGACGCGGCAGCACTTCCCATATGGACTCC‑TTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTT  >  NC_000913/4139272‑4139739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑