Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,205	C→A	T29K (ACG→AAG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,205	0	C	A	100.0%	131.5 / NA	35	T29K (ACG→AAG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/22);  total (13/22)

TGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485974‑3486446                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  ggTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGaa                                                                                                                                                                                                                                <  2:337832/250‑1 (MQ=255)         aTGTATGCAAAGGACTTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGa                                                                                                                                                                                                                        <  1:14586/251‑1 (MQ=255)         aTGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCgg                                                                                                                                                                                                                         >  2:249649/1‑250 (MQ=255)           gTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGaa                                                                                                                                                                                                                       >  1:312153/1‑250 (MQ=255)              tGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGc                                                                                                                                                                                                                   >  1:379584/1‑251 (MQ=255)                  aagtaCGTCACATTACCGTGCAGTACAGTTGATAGCCCCTGCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGta                                                                                                                                                                                                               <  2:14389/247‑1 (MQ=255)                        gTCACATTACCGTGCAGTACAGTGGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACAt                                                                                                                                                                                                         <  2:88289/251‑1 (MQ=255)                                 cagtgaaGTAAAGTTGAAAGCCCCTTCCCAGGTAGGGGGAAGAATACGTCGGAAATCCAGAGACAGCGGGGTTATATGGCTCGGGAGAAAGCGTATAAAAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAAAAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAgg                                                                                                                                                                                                <  1:18236/245‑1 (MQ=255)                                               tGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTg                                                                                                                                                                                   <  2:379584/250‑1 (MQ=255)                                                   aGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGAt                                                                                                                                                                              <  1:96733/251‑1 (MQ=255)                                                      cccTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCGGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCAGGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCaa                                                                                                                                                                           <  2:243983/251‑1 (MQ=255)                                                          ccccAGGTAGCGGGAAGCATATTTCGTCAATCCAGAGACAGCGTCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGAATTACCGCGCATGGTGCTTGGCAAACCTCAAACAGCCCCGACTCTCGAATTGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCGAGCTTATTCACCAGGGTGAAAAAGCGTAAACGCTGGACGACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGAc                                                                                                                                                                       <  1:445053/250‑1 (MQ=255)                                                              aGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa                                                                                                                                                                     >  2:328654/1‑249 (MQ=255)                                                                   gCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGt                                                                                                                                                              >  1:149542/1‑251 (MQ=255)                                                                    cGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGCCCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTa                                                                                                                                                             <  1:277865/251‑1 (MQ=255)                                                                    cGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATACGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGt                                                                                                                                                              >  2:227802/1‑250 (MQ=255)                                                                                ttCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCTACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATc                                                                                                                                                  <  2:331545/250‑1 (MQ=255)                                                                                     cAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGACAGCTTATACCAGACGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGCCTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTcc                                                                                                                                            >  1:220906/1‑251 (MQ=255)                                                                                          cAGAGACAGCGTCGTTATCTGGCTCTGGAGAAAGCTTAAAACAGAGGATAACCGCGCATTGTGCTTGGCAAACCGCAAACAGACCCGACTCGCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCGGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCGATCt                                                                                                                                       <  2:169905/251‑1 (MQ=255)                                                                                                  gcggcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAgg                                                                                                                               <  1:367279/251‑1 (MQ=255)                                                                                                        ttATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGAtt                                                                                                                         >  1:84403/1‑251 (MQ=255)                                                                                                                            cTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTg                                                                                                     >  2:27303/1‑251 (MQ=255)                                                                                                                                       ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                                                                                                          >  1:87602/1‑235 (MQ=255)                                                                                                                                       ggATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTgg                                                                                                          <  2:87602/235‑1 (MQ=255)                                                                                                                                        gATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACGGGACTCTCGAATGGCTCTTGTCTCATTGCCACATTCATACGTACCCATCCAAGAGCAAGCTTATTCACCAGGGGGCAAAAGCGGAAACGCTGTACTACATCGTTAACGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTCTCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGc                                                                                         >  1:319662/1‑251 (MQ=255)                                                                                                                                             ccGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGa                                                                                    <  1:249244/251‑1 (MQ=255)                                                                                                                                                      gTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAgcgc                                                                           <  1:260431/251‑1 (MQ=255)                                                                                                                                                        gCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCCCCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAt                                                                         >  2:475752/1‑251 (MQ=255)                                                                                                                                                                             cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCt                                                    <  1:384318/251‑1 (MQ=255)                                                                                                                                                                             cccGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTACTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCAGGGGTACGTGCGAAAACCGCCt                                                    <  2:146313/251‑1 (MQ=255)                                                                                                                                                                                    ctcGAATGGTTCTTGTCTCATTGCCACATTCATAAGTCCCCACCCAAGAGCAAGCTTATCCCCCAGGGTGAAAAAGCGGAAACGCTGTCCTAAAGCGTTAAAGGCTCTGGGGCAGTGCTGATAAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATGGGCGACCGGGGCCTTTTTGAAGGGGCCCAGAACCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGaa                                               <  1:328654/249‑1 (MQ=255)                                                                                                                                                                                       gAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGACAGGAACg                                                                                 <  1:352389/212‑1 (MQ=255)                                                                                                                                                                                       gAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATAGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGAAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGACTGTTTGAAGAGGGCCAGGCACg                                                                                 >  2:352389/1‑212 (MQ=255)                                                                                                                                                                                                 tGTCTCATGCCCACATTCATAAGTCCCCACCCAAGAGCAAGCTTATACACCAGGGTAAAAAAGCGGAAACGCTGTACTACATCTTTAAAGTCTCTGTGGCAGTGCTGATCAAAGAGGAAGAGGTAAAAAAAAGGACCCTCTCCTATCTAAATCAGGGTGATTTTATGGGCGAACTGGGCCTGTTTGAGGGGGGCCAGGAACGTAGCGCATGGGTACGTGCAAAAACCGCCTGTGAAGTGGCTGAAATTTc                                 <  2:84403/250‑1 (MQ=255)                                                                                                                                                                                                                               caaaGAGCAAGCTTATTCACCAGGGTAAAAAGGCGAAACCGCGGTCTTACATCTTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGGGGGTAAAGAAATGATCCCCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTTTTTGAAGGGGGCCAGGACCGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa  <  1:109804/249‑1 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TGTACTGCATGTATGCAAAGGACGTCACATTACCGTGCAGTACAGTTGATAGCCCCTTCCCAGGTAGCGGGAAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTA  >  NC_000913/3485974‑3486446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑