Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,139,288	C→T	D141N (GAC→AAC)	fsaB ←	fructose‑6‑phosphate aldolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,139,288	0	C	T	100.0%	170.5 / NA	51	D141N (GAC→AAC)	fsaB	fructose‑6‑phosphate aldolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/27);  total (24/27)

CCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTC  >  NC_000913/4139043‑4139526                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               ccTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGc                                                                                                                                                                                                                                           <  1:394354/251‑1 (MQ=255)      agagATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCCCCTGAACCGTACGAATGCCGTTTCCGCCCTgg                                                                                                                                                                                                                                      >  1:453026/1‑251 (MQ=255)        agaTGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGc                                                                                                                                                                                                                                    <  1:221036/251‑1 (MQ=255)           tGAGTAGTGCCACATCAGGCATTACAGTTGTGTTCGAACATCTCTATAGCTGACTCTACCGCAGGGGGGTTGAGCCTTTGTTGCGCTGCATCAAAGGGCAGGGCGATGGATTCACATCATGCCAGTAAACAGTCCAGCGCCGGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTGCTAACAGCGTTTGCAGCTCCTGAACCGTCCGAATGCCGTTTCCGCCATGGGCATc                                                                                                                                                                                                                                 >  2:299440/1‑251 (MQ=255)            gAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCt                                                                                                                                                                                                                                <  1:12611/251‑1 (MQ=255)            gAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGGTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCt                                                                                                                                                                                                                                <  1:232699/251‑1 (MQ=255)             agtagtGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTa                                                                                                                                                                                                                               <  1:199482/251‑1 (MQ=255)                    caaaaTGCGGCATTCCATTCGTGTTGGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTTAACCGTACGAATGCCGTTTCCGCCCTAGGCATCTAGGCGGtt                                                                                                                                                                                                                        <  2:68474/249‑1 (MQ=255)                     cAAATGCGGCATTCCAGTCGTGTTCGAACGTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACACCCGGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTGTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGGTTCCGCCCTGGGCATCTACGCGGTTa                                                                                                                                                                                                                       <  2:321393/251‑1 (MQ=255)                          gCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCACCCTGGGCATCTACGCGGTTAACAt                                                                                                                                                                                                                   >  1:271534/1‑250 (MQ=255)                                   cAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAAc                                                                                                                                                                                                         <  2:337617/251‑1 (MQ=255)                                   cAGTCGTGTTCGAACTTCTATATAGATGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAAc                                                                                                                                                                                                         >  1:380479/1‑251 (MQ=255)                                   cAGTCGTGTTAGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAAc                                                                                                                                                                                                         >  1:380476/1‑251 (MQ=255)                                                        ataGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGc                                                                                                                                                                                    >  1:21890/1‑251 (MQ=255)                                                        ataGCGGACTCTACCGCAGGGGGGTTGAGCATTTGTTGCGCTACATTTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGc                                                                                                                                                                                    <  2:235442/251‑1 (MQ=255)                                                         taGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCg                                                                                                                                                                                   >  1:53027/1‑251 (MQ=255)                                                              gACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGTTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACTCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCtaa                                                                                                                                                                              <  1:284505/251‑1 (MQ=255)                                                              gACTCTACCGCAGGGGTGTTGAGCATTGGTTTCGCTACATCTAAGGGCAGGGTGATGGGTTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCt                                                                                                                                                                                <  1:207175/249‑1 (MQ=255)                                                                         aGGGGTGTTGAGCATTTGTTGCGCTAAATCTAAGCGCAGGGTGAGGGATTCACATCCTGCCAGTAACCAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTCTGCCCCTGCCAGTGCGGCTAATAACCCTtgtg                                                                                                                                                                   >  2:445137/1‑251 (MQ=255)                                                                          ggggTGTTGAGCATTTGTTGCGCTACATCTAAGGGCATGGTTATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCTCCATGCTTTCTGGCGCGTGAATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGc                                                                                                                                                                                          <  1:278447/227‑1 (MQ=255)                                                                          ggggTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGc                                                                                                                                                                                          >  2:278447/1‑227 (MQ=255)                                                                           gggTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCACTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcg                                                                                                                                                                 >  1:258862/1‑251 (MQ=255)                                                                            ggTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTgcgg                                                                                                                                                                >  2:366491/1‑251 (MQ=255)                                                                                 ggatcATTCGTTGCGCGCCATATAAGTGCCGGGTGATGGATTCCCACCCGTCCAGTAAACAGTCCAGCGCCTGGCGCGGCGTTTTAAAGCTGGCTGCCAGCACCAGGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTTCGAATGCCGTTTCCGCCCGGGGCATCTGCGCGGTTAACAGACGGAACAACGTAGGTTTCCCCTTCCAGTGCGGGGAATGACCCtttgtcgtcgcta                                                                                                                                                           <  2:449316/247‑12 (MQ=255)                                                                                  gAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCtat                                                                                                                                                          >  1:207264/1‑251 (MQ=255)                                                                                         tgttgCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCGGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATAcag                                                                                                                                                    <  1:295507/250‑1 (MQ=255)                                                                                           ttgCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTcc                                                                                                                                                                                                                                             >  1:345617/1‑159 (MQ=255)                                                                                           ttgCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTcc                                                                                                                                                                                                                                             <  2:345617/159‑1 (MQ=255)                                                                                               gcTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCCCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGcc                                                                                                                                             <  2:453026/251‑1 (MQ=255)                                                                                                  ccatctacggccgtgttgttggtttCACATCCTGCCAGTAAACAGTCCAGCGCCTGACTCGGCGTTTTAAAGCTGGCTGCCCTCCCCATTCTTTCTGGCTCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCCCCCTGGGCATCTACGCGGTTAACATTCGGAGCACCGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCTCTATCTACAGCAGTTCCAAg                                                                                                                                          <  2:416625/228‑1 (MQ=255)                                                                                                     tCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt                                                                                                                                       >  1:383357/1‑251 (MQ=255)                                                                                                        aaGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAgtgt                                                                                                                                       <  1:313717/248‑1 (MQ=255)                                                                                                        aaGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCATGACGCGGCGGTTTCAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAATCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGGTAACATCCGGAACAACGTATTTTGCCCCTGCCCGTGCGGCTAATAACCCTTGTGAGGCGCTATATACAGCAGTGCCAAGTgtagg                                                                                                                                    >  2:182328/1‑250 (MQ=255)                                                                                                                   tagggATTCACCTCCGGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCCCCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGTCTAATAACCCTTGTGCGGCGCTAGAGACAGCATTGCCAAGTGTAGTAATACCctct                                                                                                                          <  2:301165/247‑1 (MQ=255)                                                                                                                           cacaTCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGt                                                                                                                 <  1:366491/251‑1 (MQ=255)                                                                                                                                    ccAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAAc                                                                                                                                                                                                                     >  2:262753/1‑142 (MQ=255)                                                                                                                                    ccAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAAc                                                                                                                                                                                                                     <  1:262753/142‑1 (MQ=255)                                                                                                                                     cAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATtg                                                                                                       <  2:271534/251‑1 (MQ=255)                                                                                                                                        tAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCCGCACCATGCTTTCTGGCGCGCGCATTTCTAACCGCGTTTGCAGCTCCTGAACCGTACGACTGCCGTTTCCGCCCTGGGCATATACGCGGtt                                                                                                                                                                                                                        >  1:290468/1‑135 (MQ=255)                                                                                                                                        tAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTGTCTGGCGCGTGCATGTCTAGCAGCTTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCAGGGCATCTTCGCGGtt                                                                                                                                                                                                                        <  2:290468/135‑1 (MQ=255)                                                                                                                                           ccaggccagggccggaggcggcgTTTTAAAGCTGGCTGCCAGCACCATGCTGTCTGGCGCGTGCATTTCTAGCAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGGTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATGTTGCCCCTGCCAGTGCGGCTAATAACCCTGGGGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCGCTTTTTTCAGTATTTTAATTGCTGCCa                                                                                                 <  2:284009/234‑1 (MQ=255)                                                                                                                                                                     tAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCCAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGCTTTTc                                                                       >  2:254707/1‑251 (MQ=255)                                                                                                                                                                     tAAAGCTGGCTGCCACCCCCATGCTATGTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACAGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTc                                                                       >  2:253264/1‑251 (MQ=255)                                                                                                                                                                       aaGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGATTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTcac                                                                     >  1:212770/1‑251 (MQ=255)                                                                                                                                                                                              ttCTGGCGAGTGCATTTGTCACAGCGTTTGCAGATCCTGAACCGTACGAATGCCGTTTCCGCCCTAGGCATCTACGCGGTTAACATAAGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACCGCCGCGCCAAGTGTAGGAATACCCTATTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTcgc                                              >  2:297602/1‑251 (MQ=255)                                                                                                                                                                                                               cTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGcttct                             >  1:314585/1‑251 (MQ=255)                                                                                                                                                                                                                     gCGTTTGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTccacc                       <  2:383357/251‑1 (MQ=255)                                                                                                                                                                                                                          tGCAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATcc                   <  1:112008/250‑1 (MQ=255)                                                                                                                                                                                                                            cAGCTCCTGAACCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATccctg                <  1:126242/251‑3 (MQ=255)                                                                                                                                                                                                                                      aCCGTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCgg     >  1:339826/1‑251 (MQ=255)                                                                                                                                                                                                                                         gTACGAATGCCGTTTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCC‑TGCGCGTCGCGGCTc  >  1:425019/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               CCTTTAGAGATGAGTAGTGCCAAATGCGGCATTCCAGTCGTGTTCGAACTTCTCTATAGCTGACTCTACCGCAGGGGTGTTGAGCATTTGTTGCGCTACATCTAAGGGCAGGGTGATGGATTCACATCCTGCCAGTAAACAGTCCAGCGCCTGACGCGGCGTTTTAAAGCTGGCTGCCAGCACCATGCTTTCTGGCGCGTGCATTTCTAACAGCGTTTGCAGCTCCTGAACCGTACGAATGCCGTCTCCGCCCTGGGCATCTACGCGGTTAACATACGGAGCAACGTATTTTGCCCCTGCCAGTGCGGCTAATAACCCTTGTGCGGCGCTATATACAGCAGTGCCAAGTGTAGTAATACCCTCTTTTTTCAGTATTTTAATTGCTGCCAGACCTTCGGAAGTCACCGGGATTTTCACCACAATACCCGGAATAGCGTCGCGCAGGCGCTTCGCTTCTTCCACCATCCCCTGCGCGTCGCGGCTC  >  NC_000913/4139043‑4139526

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑