Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	536,492	A→G	intergenic (+6/‑94)	hyi → / → glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	536,492	0	A	G	100.0%	246.1 / NA	64	intergenic (+6/‑94)	hyi/glxR	hydroxypyruvate isomerase/tartronate semialdehyde reductase 2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/29);  total (35/29)

TTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCA  >  NC_000913/536245‑536737                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tttATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACg                                                                                                                                                                                                                                                    >  1:356915/1‑251 (MQ=255)       atatGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTc                                                                                                                                                                                                                                              >  1:134193/1‑251 (MQ=255)                  aTGGAAGGTGAATTAACCAACGCCATGAGTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGGCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATGAATTATGATTATCGCGTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGCGTGAATAGAAACCCCAAACCACCACTGAAGCCGGCTTACGCTGGATGAATCCGTACCGGTAAAACGTGACGCTATTCAGACAATGCtt                                                                                                                                                                                                                                   <  1:448242/251‑1 (MQ=255)                     gAAGGTGAATGAAACAACCCCATGACTCAGTGAGCTGATAAAATTGGTCACCTGCAAATAGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGAGTGTGAATCTAATCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAATCGTGACGCTATTCAGACAATGCttttt                                                                                                                                                                                                                                <  2:260107/251‑1 (MQ=255)                                        ccATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGc                                                                                                                                                                                                               >  2:67935/1‑249 (MQ=255)                                             aCTCAGTGGGCGGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTAGCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGAt                                                                                                                                                                                                        <  2:389385/251‑1 (MQ=255)                                                cAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATc                                                                                                                                                                                                       <  1:339459/249‑1 (MQ=255)                                                cAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCgg                                                                                                                                                                                                     >  1:297521/1‑250 (MQ=255)                                                            aaaaTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAAt                                                                                                                                                                                          <  2:381537/250‑1 (MQ=255)                                                               aTTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGCTCGTGTTGTccgacttc                                                                                                                                                                                        >  2:410812/1‑242 (MQ=255)                                                                  ggTCCCCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGa                                                                                                                                                                                    <  2:479744/250‑1 (MQ=255)                                                                     cACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAg                                                                                                                                                                                <  1:236064/251‑1 (MQ=255)                                                                          gCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGa                                                                                                                                                                             <  1:395376/249‑1 (MQ=255)                                                                             aaTTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTCTCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAttt                                                                                                                                                                        >  2:230736/1‑251 (MQ=255)                                                                             aaTTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAAttt                                                                                                                                                                        <  1:390831/251‑1 (MQ=255)                                                                                tGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTaa                                                                                                                                                                      <  2:50178/250‑1 (MQ=255)                                                                                                   ggcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACt                                                                                                                                                  <  1:230736/251‑1 (MQ=255)                                                                                                   ggcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACt                                                                                                                                                  <  1:67935/251‑1 (MQ=255)                                                                                                    gcgAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTg                                                                                                                                                 >  2:172008/1‑251 (MQ=255)                                                                                                          cgggaacaggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGCAACTTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGAttt                                                                                                                                           >  2:31321/1‑251 (MQ=255)                                                                                                           gggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTa                                                                                                                                          <  2:134193/251‑1 (MQ=255)                                                                                                           gggaaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTa                                                                                                                                          <  1:123940/251‑1 (MQ=255)                                                                                                              aaccggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTg                                                                                                                                       >  1:105017/1‑251 (MQ=255)                                                                                                               accggAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTgg                                                                                                                                      <  1:441957/251‑1 (MQ=255)                                                                                                                     gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAGTTCTGACTACAACGGTTGGGTTAGGTGAGACAATACACCCCAAACCACCACAGCAGCCGGTCTACGCTGGCTGGATCAGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTATTTAGGCTGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGaaa                                                                                                                                                                                  >  1:478310/1‑201 (MQ=255)                                                                                                                     gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTTTTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                        <  2:148629/195‑1 (MQ=255)                                                                                                                     gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTTTTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTc                                                                                                                                                                                        >  1:148629/1‑195 (MQ=255)                                                                                                                     gaAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTCGGGTGTGAATATAAACCCCGAACCGCCGCTGAAGCCGGTTTACGCTGTATGGATCCGTATCGTTAAAGCGTGACGCTATTCAGACAAAGCTTTTTTAGGCGTCTAAGTGGGCAGGGGATCGTGTTTTCCGAATTCAGGaaa                                                                                                                                                                                  <  2:478310/201‑1 (MQ=255)                                                                                                                              tATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgt                                                                                                                         >  1:160891‑M1/1‑247 (MQ=255)                                                                                                                               aTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAg                                                                                                                                 >  1:333429/1‑240 (MQ=255)                                                                                                                               aTGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAg                                                                                                                                 <  2:333429/240‑1 (MQ=255)                                                                                                                                  aTTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgt                                                                                                                   >  1:442720‑M1/1‑243 (MQ=255)                                                                                                                                    tATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAgggg                                                                                                                                                                                                          <  2:83065/162‑1 (MQ=255)                                                                                                                                    tATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAgggg                                                                                                                                                                                                          >  1:83065/1‑162 (MQ=255)                                                                                                                                       ctctTTAAGGTAATCGAAACTTCTGCCCGCACCGGTTGGGTTGGGTGTGAATATAAACCCCAAACCATCACGAAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGCGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggc                                                                                                               >  2:477190‑M1/1‑238 (MQ=255)                                                                                                                                       ctctTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggc                                                                                                               >  2:264080‑M1/1‑238 (MQ=255)                                                                                                                                         ctTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACTCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCGGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggc                                                                                                               <  1:477190‑M1/248‑13 (MQ=255)                                                                                                                                            tAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattag                                                                                                          <  2:65700‑M1/250‑18 (MQ=255)                                                                                                                                             aaGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagac                                                                                                        >  1:107304‑M1/1‑232 (MQ=255)                                                                                                                                                gTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatt                                                                                                     >  1:32234‑M1/1‑229 (MQ=255)                                                                                                                                                  aaTCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttg                                                                                                   >  1:5130‑M1/1‑227 (MQ=255)                                                                                                                                                  aaTCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtaggcgtaggcattagacatttg                                                                                                   <  2:423487‑M1/251‑25 (MQ=255)                                                                                                                                                       aaaaTTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTCAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGCTTTATTGGCTTAGGCATTAgtagtcgtaggcattcgacatttgtgcct                                                                                              >  2:134618‑M1/1‑222 (MQ=255)                                                                                                                                                       aaaaTTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcct                                                                                              <  2:297521‑M1/251‑30 (MQ=255)                                                                                                                                                             cTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCCGGGGCTCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgattcg                                                                                        >  2:217393‑M1/1‑216 (MQ=255)                                                                                                                                                               gACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgac                                                                                      <  2:356915‑M1/251‑38 (MQ=255)                                                                                                                                                                   aCAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGACGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGCTCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgctt                                                                                  >  1:300300‑M1/1‑210 (MQ=255)                                                                                                                                                                                 gggtGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgttcctgatgcgacgcttgacgcgtcttatc                                                                     <  2:105017‑M1/250‑55 (MQ=255)                                                                                                                                                                                   gtgtGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTTGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattaggcatttgtgcctgatgcgacgcttgacgcgtcttatcagg                                                                  <  1:443521‑M1/251‑58 (MQ=255)                                                                                                                                                                                   gtgtGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcagg                                                                  >  1:264573‑M1/1‑194 (MQ=255)                                                                                                                                                                                    tgtgAATATAAACCCCAAACCACCCCGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggc                                                                 >  1:369232‑M1/1‑193 (MQ=255)                                                                                                                                                                                             aaaCCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccg                                                        >  1:188675‑M1/1‑184 (MQ=255)                                                                                                                                                                                                  ccAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATGCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacagttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgcc                                                   <  2:273818‑M1/251‑73 (MQ=255)                                                                                                                                                                                                         accacGGCAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccg                                            >  1:138614‑M1/1‑172 (MQ=255)                                                                                                                                                                                                              ggAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTCGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggagccgcatccggcaat                                       >  1:66710‑M1/1‑167 (MQ=255)                                                                                                                                                                                                                  gCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggt                                   >  1:79301‑M1/1‑163 (MQ=255)                                                                                                                                                                                                                   ccGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtg                                  <  2:442720‑M1/251‑90 (MQ=255)                                                                                                                                                                                                                     ggTTTACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgc                                 >  1:15075‑M1/1‑160 (MQ=255)                                                                                                                                                                                                                         tACGCTGGATGGATCCGTACCGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGACTTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaat                            >  2:300999‑M1/1‑156 (MQ=255)                                                                                                                                                                                                                          aCGCTGGATGGATCCGTACCGTTAAAACGTGAAGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcaaaatg                           >  2:466251‑M1/1‑155 (MQ=255)                                                                                                                                                                                                                                             cGTTAAAACGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacg        >  2:59639‑M1/1‑136 (MQ=255)                                                                                                                                                                                                                                                 aaaaCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcat     <  2:160891‑M1/250‑119 (MQ=255)                                                                                                                                                                                                                                                  aaaCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatct   >  1:278951‑M1/1‑131 (MQ=255)                                                                                                                                                                                                                                                   aaCGTGACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCCGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTAgtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatctt  >  1:58151‑M1/1‑130 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TTTATCATATGCAGCGGATGGAAGGTGAATTAACCAACACCATGACTCAGTGGGCTGATAAAATTGGTCACCTGCAAATTGCCGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTATGATTATCTCTTTAAGGTAATCGAAAATTCTGACTACAACGGTTGGGTTGGGTGTGAATATAAACCCCAAACCACCACGGAAGCCGGTTTACGCTGGATGGATCCGTACCGTTAAAACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGAAATTTAAAAGAGGTTAATTATGAAACTGGGATTTATTGGCTTAGGCATTATGGGTACACCGATGGCCATTAATCTGGCGCGTGCCGGTCATCAATTACATGTCACGACCATTGGACCGGTTGCTGATGAATTACTGTCACTGGGTGCCGTCAGTGTTGAAACTGCTCGCCA  >  NC_000913/536245‑536737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑