| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,628,332 | T→C | intergenic (+69/‑20) | ydfG → / → rspR | 3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,628,332 | 0 | T | C | 94.7% | 63.5 / ‑6.5 | 19 | intergenic (+69/‑20) | ydfG/rspR | 3‑hydroxy acid dehydrogenase YdfG/DNA‑binding transcriptional repressor RspR |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (6/12); minor base G (0/1); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAA > NC_000913/1628087‑1628550 | aaaGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGGCACAAAAAAGTGGTAGACTCATGCAGTTAacccact < 2:388346/250‑1 (MQ=255) gAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcagg > 1:378672‑M1/1‑232 (MQ=255) aaTACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttc < 1:176779‑M1/251‑35 (MQ=255) aCCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctca < 2:337572‑M1/251‑38 (MQ=255) gAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagtt < 1:131417‑M1/251‑68 (MQ=255) gtgtCAACGCTGCCTGCTCACGTCAAGATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTTAATGTCCACCGGCAGTAATTTTGATACCCGGCGTAACTGCCTGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACGCACTCACttcttgaactgcgtcaggacctttatgttcgttctcaataccaaattcaactgcctgttttgcattttatgttttgaaatcg < 2:234082‑M1/251‑83 (MQ=255) gCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgtttttaaatcgttgctctg < 1:132091‑M1/251‑91 (MQ=255) gTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctc < 1:297518‑M1/211‑64 (MQ=255) gTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggcccgttctcaataccaaattcaacttcctgattctc > 2:297518‑M1/1‑148 (MQ=255) tatcaataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacc < 2:393055‑M1/164‑22 (MQ=255) tatcaataCCCTGGAAATGATGCCGGTTAACCAAAGCTATGCCGGACTGAATGTCCACCGTCCGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacc > 1:393055‑M1/1‑143 (MQ=255) ataCCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacc > 1:376262‑M1/1‑138 (MQ=255) gCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccat < 1:211824‑M1/250‑129 (MQ=255) gTTACCCAAAGCTATGCCGGGCTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccacctttc < 2:41393‑M1/251‑134 (MQ=255) aTGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaa < 2:378672‑M1/250‑146 (MQ=255) ggACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaa < 2:376262‑M1/251‑152 (MQ=255) tGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctt > 1:391079‑M1/1‑92 (MQ=255) tGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctt < 2:391079‑M1/223‑132 (MQ=255) cacaAAAAAGTGGTAGACTCATGCAGTTAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttaaaccatttcactaaaccagtcattttgttagacataattattacc < 2:356768‑M1/250‑212 (MQ=255) ttAACCCACTCACtacatgaacagcggcaggacctttaggtccgttctcaataccaaattcaacttcctgattctcagttaatgttttgaaatcgttgctctgaattgctgagaaatagacaaacacatctttgctgccatctttcggcgtgatgaaaccaaaacctttttcagggttcaa > 1:84844‑M1/1‑13 (MQ=255) | AAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAGATGTCAGCGAAGCCGTCTGGTGGGTGTCAACGCTGCCTGCTCACGTCAATATCAATACCCTGGAAATGATGCCGGTTACCCAAAGCTATGCCGGACTGAATGTCCACCGTCAGTAATTTTTATACCCGGCGTAACTGCCGGGTTATTGCTTGTCACAAAAAAGTGGTAGACTCATGCAGTTAACTCACTCACAAGCAAGAACGAATGACCGTCGAAACGCAACTTAATCCCACACAGCCTGTCAATCAGCAGATTTATCGTATTCTTCGTCGCGACATTGTCCATTGCCTGATTGCTCCAGGCACACCGTTGTCGGAAAAAGAAGTTTCTGTTCGTTTCAATGTGTCACGCCAGCCGGTTCGTGAAGCCTTTATTAAACTGGCGGAAAACGGCCTGATTCAAA > NC_000913/1628087‑1628550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |