breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,833,5670TG42.1% 49.4 / 10.2 57G364G (GGT→GGGnorVanaerobic nitric oxide reductase flavorubredoxin
*NC_000913442,0640CT33.3% 42.2 / 12.7 36F172F (TTC→TTTthiItRNA uridine 4‑sulfurtransferase
*NC_0009133,177,7480CA33.3% 47.5 / 11.1 41E55* (GAG→TAG) nudFADP‑sugar pyrophosphatase
*NC_000913501,5640AC32.4% 62.1 / 11.4 37*559E (TAA→GAA) ybaLputative transporter YbaL
*NC_0009133,824,7320TG31.7% 136.3 / 12.2 83V73G (GTG→GGG) trmHtRNA (Gm18) 2'‑O‑methyltransferase
*NC_0009134,056,1150AC30.9% 58.7 / 12.3 44G75G (GGT→GGGglnLprotein histidine kinase NtrB
*NC_000913494,5500GC28.9% 81.6 / 11.6 45R49P (CGG→CCG) recRrecombination mediator protein RecR
*NC_0009134,192,4250CG28.1% 103.4 / 10.0 58G71R (GGG→CGG) thiG1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase
*NC_0009134,223,5970CT28.0% 83.9 / 15.9 50R11K (AGA→AAA) iclRDNA‑binding transcriptional repressor IclR
*NC_00091367,7570TC23.2% 184.3 / 12.0 85D194G (GAT→GGT) araAL‑arabinose isomerase
*NC_0009132,846,7050GC21.4% 141.8 / 12.0 70V236V (GTC→GTGhycDformate hydrogenlyase subunit HycD

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4317019 =109 (2.370)3 (0.070) 3/470 3.0 2.9% coding (200/1137 nt) phnM RPnTP hydrolase
?NC_000913 4317231 = 92 (2.030)coding (665/681 nt) phnL methylphosphonate degradation complex subunit PhnL
* ? NC_000913 3583428 =NA (NA)3 (0.070) 3/476 3.0 5.4% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4283908 53 (1.150)coding (995/1650 nt) actP acetate/glycolate:cation symporter
* ? NC_000913 846798 =37 (0.810)3 (0.070) 3/468 3.0 5.8% coding (322/660 nt) glnP L‑glutamine ABC transporter membrane subunit
?NC_000913 = 3925239 61 (1.350)coding (395/1890 nt) mnmG 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG