Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,192,425	0	C	G	28.1%	103.4 / 10.0	58	G71R (GGG→CGG)	thiG	1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (26/15);  new base G (0/16);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 9.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTGGT  >  NC_000913/4192177‑4192674                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                          cgcgcCGAGCGGCATCCCCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCgg                                                                                                                                                                                                                                                         <  2:285471/251‑1 (MQ=255) cgcgcCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCgg                                                                                                                                                                                                                                                         >  1:411771/1‑251 (MQ=255)  gcgcCGAGCGGCAGCCCCGCTGCACAGCATACTTCTTCCAGACTGTTACACAATACCGGAGCGGCCCCGCAGTAAGGCATCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACATCCAGCGGGCGTTAGGGTGAATCTCTAAGTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCATATGTGCGGCGAAAA‑TGGCCGCTTCCGCTGTTGTCTCCCCGGa                                                                                                                                                                                                                                                        <  2:300436/251‑1 (MQ=255)         gcgtcATCACCGCTGCACAGCCGACTTCTTCCAGGCGTTTCCACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAAGCCCAGGTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTATCCAGTTTG‑TGCCTAACGCTTCACGTGCCTGATGGACTGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTAttt                                                                                                                                                                                                                                                 <  1:290123/247‑1 (MQ=255)           ggCATCACCGCTGCAAAGCCGCCTTCATCCAGACGTTTACACAATCCAGGATCGACCCCGCAGTACGGCAGCACGACAAATCCCTGTTGTAACAGCGTTTCGCCGGCTGTCAGGGATTCGCTGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGCATCTCTAGTTTTAACCAGTTTG‑TGCCTAACGCTACCCGAGCCAGATGTGCGGCGAACA‑TGGCTTCTTCCGATGTTTTCGCACCGGATATATCTgg                                                                                                                                                                                                                                               >  2:433636/1‑251 (MQ=255)              aTCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGGTCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTCTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGgc                                                                                                                                                                                                                                              >  1:322687/1‑249 (MQ=255)                   cGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAggg                                                                                                                                                                                                                                       >  1:278045/1‑251 (MQ=255)                    gCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGCTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAgggg                                                                                                                                                                                                                                      >  1:244724/1‑250 (MQ=255)                       gcacacccgccatcttcCAGACGTTTCAACAATACAGGCTCGGCCCCGCAGTACGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGCTCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTccc                                                                                                                                                                                                                                   >  2:304534/13‑249 (MQ=255)                         acaGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCacac                                                                                                                                                                                                                                 >  1:185384/1‑251 (MQ=255)                          caGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTGTTCGCCCCGGATGTATTTGGCAGCAGGTTCACAcc                                                                                                                                                                                                                                <  1:179915/251‑1 (MQ=255)                              cGACTTCTTCCAGACGTTTACACAATCCCGGAGCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGTTCGGGCAAAAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAGCCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGTCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCTCGGATGTATTGGGCAGCAGGGTCGCACccgcc                                                                                                                                                                                                                            <  2:319594/251‑1 (MQ=255)                                       ccaaacttttACACAAGTCCGGGTCGGCCCCGCAATAGGGCAGCGCGACAAATTCCGGTTGTACCAGTGTTGCGGGGGCTTCCAGGGTTTCGAGGGGATCGGGCAACAGCAAGCGGGGGTTAGGGTGAATCTCAAATTTTAACCGGTTTG‑TGCCTAACGCTTCACGAGCCAGAGGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTTGCCCGGGATGTATTTGGCGGCGGGGTCCCACCCGCCGCGATAAGc                                                                                                                                                                                                                   <  2:72295/244‑1 (MQ=255)                                        cAGACTTTTACACAATACCGGATCGGCCCCGAAGTAAGGCAGCACGACAAATCCCTGTTGTCCCAGCGTTTCGGCGGCTTTCAGGGTTTTGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCg                                                                                                                                                                                                                  <  1:27879/251‑1 (MQ=255)                                             gTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGCTCg                                                                                                                                                                                                             >  1:109052/1‑251 (MQ=255)                                                              ccgcccccGCAGAAGGGCAGCCCAACAAACCCTTGTGGTACCGGCGTTTCGGCGGTTTTCGGGGTTTCGAGGGGAGCGGGCAACGGCCAGCGGGCGTCAGGGGGATTCTCTAATTTAAACCAGTTGG‑TGCCTACCGCTTCACGAGCCAGAGGGGCGGCGAAAA‑TGGCTTCTCCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCGGGGTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTtgtg                                                                                                                                                                                            <  2:353991/247‑1 (MQ=255)                                                                 gCCCCGCAGTACGGCAGAATGACAAATCCCTGTTGTTCCAGTGTTTGGGCGGCTTTAAGGGTTCCGATGGGTTCGGGCAACAGCCAGGGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGGGGCGAAAA‑GGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGAGGGGTAACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTg                                                                                                                                                                                         <  1:107384/251‑1 (MQ=255)                                                                 gCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTg                                                                                                                                                                                         >  1:287517/1‑251 (MQ=255)                                                                  ccccGCAGTAAGGCAGCCCGACAAATCCCTGTTGTACCGGCGTTTCGGCGGTTTTCAGGGTTTCGATGGGATCGGGCAACGGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACCCTTCACGAGCCGGAGGGGCGGCGAAAA‑TGGCTTCTCCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCGGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTgg                                                                                                                                                                                        <  1:412817/251‑1 (MQ=255)                                                                           aaGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGAGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCg                                                                                                                                                                               >  1:265511/1‑251 (MQ=255)                                                                                cgagcgacaactctgggttgtACCAGCGTTTGGGGGGTTTTCAGGGTTTCGATGGGCTGGGGCACCAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTGG‑TGCCTAACTCTTCAGGAGCCAGAGGGGCGGCGAAAA‑GGGTTTCTTCCGCTGTTTGCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACg                                                                                                                                                                          <  2:396746/235‑1 (MQ=255)                                                                                 gcaCGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGAGGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGt                                                                                                                                                                         <  1:246413/251‑1 (MQ=255)                                                                                    cGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTc                                                                                                                                                                      >  1:4012/1‑251 (MQ=255)                                                                                     gACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGt                                                                                                                                                                                                                                      >  2:379082/1‑186 (MQ=255)                                                                                     gACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTGTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGt                                                                                                                                                                                                                                      <  1:379082/186‑1 (MQ=255)                                                                                        aaaTCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCACGTCGACACGTTTCATCg                                                                                                                                                                  >  2:346303/1‑251 (MQ=255)                                                                                           ccccTGTTGTACCAGCGTTTCGGCGGCTTTAAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTACCGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCa                                                                                                                                                               <  1:95913/250‑1 (MQ=255)                                                                                                gttgtACCAGCGTTTGGGCGGTTTTCGGGGTTTCGATGGGAGCGGGCAACAGCCAGCGGGCGTCGGGTTGAATCTCAAATTTTACCCAGTTTT‑TGCCTAACGCTTCACGGGCGAGATGGGGGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGTTGTTTTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAgtgt                                                                                                                                                           <  1:431513/250‑1 (MQ=255)                                                                                                 ttgtACCAGCGTTTCGGCGGCTTTCGGGGTTTCGATGGTATGGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTACCGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTc                                                                                                                                                          <  1:8537/250‑1 (MQ=255)                                                                                                    tACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCAcc                                                                                                                                                       >  1:7547/1‑250 (MQ=255)                                                                                                    caccggggTTTGGGCGGCTTTGAGGGTTTGGAGGGGATCGGGCAACAGCCGGGGGGCGGCGGGGTGAATCACTAATTTTAACCAGTTTG‑TGCCTACCGCTTCACGAGCGAGGTGGGCGGCAAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCGGGGTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCa                                                                                                                                                      <  2:11079/244‑1 (MQ=255)                                                                                                      ccagggtgtCGGGGGCTTTCAGGGTTTGGAGGGGCTCGGGCAACAGCCAGGGGGCGGCAGGGTGAATCTCTATTTTTAACCTTTTTG‑GTCCAAACGCTTCACGAGCAAGGGGGGCGGCGAAAA‑GGGCTTCTTCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCGGGGTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAgc                                                                                                                                                    <  2:185384/243‑1 (MQ=255)                                                                                                       cggggttggtgtgcgtttCAGGGTTGCGGGGGGACCGGGCAACGGCCGGTGGGCGTCAGGGTGACTCTTTAATTGTAACC‑GTTTGGTGCAAAACTTTTCTCGGCCCAGATGGGCGTCTAAAA‑GGGCTTCTTCCGCTTTTTTCGCCCGTGTTGGATTTGGCATGAGGTGCCCCGCCGCCGCTATAAGCGTTTCGAGGATAGCGTCGTTGTGCTGGCGCAATGCGTCACGTTTCATCTCCAGTGTCAGCAgct                                                                                                                                                   <  2:429414/236‑1 (MQ=255)                                                                                                           gTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAgctggct                                                                                                                                               >  1:11539/1‑251 (MQ=255)                                                                                                                      tttCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAgcgc                                                                                                                                    >  1:100857/1‑251 (MQ=255)                                                                                                                           gggTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATc                                                                                                                               >  1:388083/1‑251 (MQ=255)                                                                                                                                              aaaCAGCCAGAGGGCGTAAGGGGGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTCTTTGGCAGCAGGGTCAAACCCGCCGCGCTACGCGGTTCGAGGATCGCAGCGTTGAGCTGGCGCACGTCGACACGTTTCCTCGCCAGTGTAACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCCGTtgtg                                                                                                            >  2:296079/2‑251 (MQ=255)                                                                                                                                                     cagcGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGc                                                                                                     >  1:120957/1‑251 (MQ=255)                                                                                                                                                        cGGGCGCCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGACAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATACGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGCTCGCCTCCACCATCAGTTGTGAAGAAGCGa                                                                                                   >  1:416875/1‑250 (MQ=255)                                                                                                                                                                ggggTGAATCTCTAATTTTACCCATTTTG‑TGCCTAACGCTTCACGACCCAGATGGGGGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCGGGATGTATTTGGCAGCGGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCtg                                                                                          <  1:394293/250‑1 (MQ=255)                                                                                                                                                                aGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCtg                                                                                          >  1:313187/1‑251 (MQ=255)                                                                                                                                                                  ggTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCGGGATGTATTGGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCATCGTtg                                                                                                                                                                                              <  1:132938/149‑1 (MQ=255)                                                                                                                                                                  ggTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGATGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTtg                                                                                                                                                                                              >  2:132938/1‑149 (MQ=255)                                                                                                                                                                  ggTGAATCTATAGTTTTAACTGGTTTG‑TCCCTAACGCTTCTCG‑GGCAGGTGGGGGGGGAAAAATGGCTTCTCCCGCTTTTTGCGCCCCGGATGTATTTGGCAGCTGGGCCCCCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAAGTTCCCtgtg                                                                                        <  2:441982/251‑1 (MQ=255)                                                                                                                                                                    tGAATTTCTATTTTTAACCAGTTTG‑GGCCAACCGTTTCACGAGCAAGATGGGCGGCGAAAT‑TGCCTTCTCCCGCTTTTTTCGCCCGGGAGGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGc                                                                                       <  2:445237/250‑1 (MQ=255)                                                                                                                                                                     gAATCTCTAATTTTACCCGGTTTG‑GGCCTACCGCTTCCCGAGCCAGAGGGGCGGCGAAAA‑TGCCTTCTCCCGCTTTTTCCGCCCGGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGcc                                                                                      <  1:4979/250‑1 (MQ=255)                                                                                                                                                                     gAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGATTCACGAGCCAGAAAGGCAGCGAAAA‑TGGCTTCTTCCGCTGTTTTAGCCCCGGATGTATTTGGCAGAAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCAAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGATGTGAAGAAGCGAATTTACCTGTGCCg                                                                                     >  1:227519/1‑251 (MQ=255)                                                                                                                                                                     aaaTCTCTAATTTTACCCGGTTTG‑TGCCTAACGCTTCCCGGGCCGGAGGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCGGGAGGTATTGGGAGGCGGGGTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCg                                                                                     <  2:400425/250‑1 (MQ=255)                                                                                                                                                                     aaaTCTCTAATTTTAACCAGTTTG‑TGCCTACCGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGcc                                                                                      <  1:268389/249‑1 (MQ=255)                                                                                                                                                                                   aaCCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCCCCAGCTGGCTGCCGGACGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGAtgtg                                                                        >  2:262298/1‑250 (MQ=255)                                                                                                                                                                                                      gCTTCACGAGACAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGAGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGc                                                     >  1:450410/1‑251 (MQ=255)                                                                                                                                                                                                             gAGCAAGATGGGCGGCGAAAA‑TGGCTTTCTCCGCTGTGTTCGCCCGGGATGTATTTGGCAGGGGGGTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGGTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGt                                              <  2:322687/251‑1 (MQ=255)                                                                                                                                                                                                              ggccagtggggCGGCGAAAT‑GGGCTTCCTCCGCTGTTTCCGCCCCGGATGGTTTGGGCGGCGGGTTCACCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGGGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTa                                             <  1:49547/243‑1 (MQ=255)                                                                                                                                                                                                                   gtgggggggcgAAAA‑TGTCTTGTCCCGTTGTTTTCGCCGGGGATGTTTTGGGCAGGGGGGTCCCCCCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACAtt                                        <  2:7547/244‑1 (MQ=255)                                                                                                                                                                                                                      gggCGGCGAAAA‑GGTCTCCTCCCGCTGTTTTCGCCCGGGTTGTGTTGGGCAGCGGGGTCCCACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCa                                     <  2:87782/251‑1 (MQ=255)                                                                                                                                                                                                                                   tGGCTTCCTCCGCTGTTTTCGCCCAGGATGTAATTGGCAGAAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACAAGCTGGCTGCAGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGCAAACAGATGGGAATCAAACGTTTTGTCCGCAATACGTAACATTGCAACCCCCTGCAAt                         >  1:160110/1‑251 (MQ=255)                                                                                                                                                                                                                                     gCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATa                        >  2:383815/1‑250 (MQ=255)                                                                                                                                                                                                                                        tctcccGCTTTTTTCGCCCGGGATGTATTTGGCAGGAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAAc                      <  1:398265/245‑1 (MQ=255)                                                                                                                                                                                                                                                        ccccGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTg    >  1:112241/1‑251 (MQ=255)                                                                                                                                                                                                                                                        ccccGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAAGACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTg    <  1:304534/251‑1 (MQ=255)                                                                                                                                                                                                                                                          ccGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTGGt  >  1:10538/1‑251 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                          CGCGCCGAGCGGCATCACCGCTGCACAGCCGACTTCTTCCAGACGTTTACACAATACCGGATCGGCCCCGCAGTAAGGCAGCACGACAAATCCCTGTTGTACCAGCGTTTCGGCGGCTTTCAGGGTTTCGATGGGATCGGGCAACAGCCAGCGGGCGTCAGGGTGAATCTCTAATTTTAACCAGTTTG‑TGCCTAACGCTTCACGAGCCAGATGGGCGGCGAAAA‑TGGCTTCTTCCGCTGTTTTCGCCCCGGATGTATTTGGCAGCAGGGTCACACCCGCCGCGATAAGCGGTTCGAGGATAGCGTCGTTGTGCTGGCGCAAGTCGACACGTTTCATCGCCAGTGTCACCAGCTGGCTGCCGGAAGCGCGGATCGCCTCCACCATCAGTTGTGAAGAAGCGAATTTCCCTGTGCCGGTAAACAGATGTGAATCAAACGTTTTGTCCGCAATACGTAACATTTCAACCCCCTGCAATAACCTGAAAAAGCAGGATCTGGT  >  NC_000913/4192177‑4192674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑