| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 442,064 | 0 | C | T | 33.3% | 42.2 / 12.7 | 36 | F172F (TTC→TTT) | thiI | tRNA uridine 4‑sulfurtransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/19); new base T (12/0); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAA > NC_000913/441826‑442309 | aaGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCCGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCgccccgcccc > 1:56622/1‑242 (MQ=255) cGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAg < 2:345714/251‑1 (MQ=255) tGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATTGGCACCCCGGa > 1:313733/1‑250 (MQ=255) ttCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCCATAGGCAccccgggag > 1:468351/1‑243 (MQ=255) tATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGCACGGCACCCAGGAAGATGGGCt > 1:371089/1‑251 (MQ=255) cagatGGAAGGTAAAACCTTCTGCGTACGCGTTAAGCGCCGTGGCAAACATTATTTTAGCTCGATTGATGTGGAACGTTACGTCTGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTTGAAGACGATCGTCTCCTGCTGATTAAAGTCCGCTACGAATGTATTGGCGTTTTCCCGATCGGCACCCAGCAAGATGTGCTGTCTCTCAt < 2:186549/247‑1 (MQ=255) ggAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑cc < 1:345648/250‑1 (MQ=255) gCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTTGAACGTTACGTCGGCGGCGGTTTAAATCAGCAGATTGAATCCGCGCGCGTGAAGCTGGTCAATCCGGATGTGACTGGCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCTCTACGAAGGTATTGGCGGTTGCCCGAGCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑GCGGTGGTTTCTACTcc < 2:396984/248‑1 (MQ=255) gCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGgt < 2:468351/251‑1 (MQ=255) cgcgTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGTTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑c < 1:435128/251‑1 (MQ=255) aaGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGGTCCGCACCCACGAAGATGTGCTGTCGCTCCTTT‑CCGTGGGTTTCGACTCCCGGGTTT‑CCAGttt > 2:332210/1‑250 (MQ=255) gCAAACATGATGTTAGCGCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAACCAGCAGATTGAACCCGCGCGTGTGAAGCTGACCAATCCTGACGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCgg < 1:19107/181‑1 (MQ=255) gATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑TCGGTGGTTTCGACCCCGGGGTTT‑CCAGTTATATGTTGATGCGTCGCgg > 1:411487/1‑251 (MQ=255) tttAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCggtgg < 1:465653/206‑1 (MQ=255) aGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCcg < 2:61581/251‑1 (MQ=255) cGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTg < 1:292351/251‑1 (MQ=255) tGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCg < 1:438519/181‑1 (MQ=255) aaCGTTACGTCGGCGGCGGTTTAAAGCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGcttc < 2:391857/251‑1 (MQ=255) aCGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGGGTTT‑CCAGTTATAGGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGcttct < 1:455634/251‑1 (MQ=255) cGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGGTTCCCGATCGGGACCCAGGAAGATGTGCTGTCGCTCATTT‑TCGGGGGGTTCGACTCCGGGGGTT‑CCCGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCCTTACTGgttctt > 1:306983/1‑251 (MQ=255) ggcggcggTTTAAATCAGAATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGCTGTGACTGTCCATCTTGAAGAGGAAGACGATAGTCTCCTGCTGGTTACAGGCCGCTACGAAGGTATTGGCGGTTTTCCCATCGGCACCCCGGGAGATGAGGTGTCGCTCATTTTCTGGGGGTTT‑GTCTCCGGGGTTTTCCCGTTAT‑TGTTGTTTCGGCGCGGCGGCCGCGCGGACTAATGCTTCTTTTACCttcg > 2:281454/1‑248 (MQ=255) tAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGgcggcgcggc < 2:227330/250‑1 (MQ=255) tCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGTTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTcc < 1:373174/172‑1 (MQ=255) ttGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTTTCCGG‑GGTTTCGCCTCCGGCGTTT‑CCCGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTggg > 1:52244/1‑250 (MQ=255) cgcgTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATCGGCACCCAGGAAGATGTGCTGTCGCCCATTT‑TCGGTGGTTTCGACTCCGGGGGTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAggg > 1:171962/1‑250 (MQ=255) gTGAAGCTGACCAATCCGGATGTGCCTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCACGAAAGTGTGCTGGCGCTCATTT‑CCGGTGGTTTCCACTCCGGTGTTT‑CCCATTATATGTTGTTGCGTCGCGGGTGCCCCGTGCATTACTGCTTTTTTTAACTCGGCGGCGCGGCGGCGGGAATTGGCGTGCGGCAgggggg > 2:320539/1‑247 (MQ=255) gTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATCGGCAACCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGGTTCGACTCCGGTGTTT‑CCAGGTATATGTTGATGCGTCGCGGCTTCCGCGTGCGTTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGCGCGTCAgggggg > 1:203460/1‑247 (MQ=255) gCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCAtt < 1:253034/251‑1 (MQ=255) tGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTTCCGATCGGCCCCCAGGAAGATGTGCTGTCGCTCCTTT‑CCGGGGGTTTCGGCTCCGGTGTTT‑TCAGTTATATGTTGATGCGTCGCGGCTGCCGCGGGCATTAATGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGGGCGGCAGGGGGGGCATTAt > 1:440493/1‑251 (MQ=255) cAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCCCCCCGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTTGACTCCGGGGTTT‑TCCGTTATATGTTGTTGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTTACCTCGGCGGCGCGGCGCATGAAATTGGCGGGCGTCTGGGGGGGCATTa > 2:74768/1‑246 (MQ=255) cAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCTGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTa < 1:74768/246‑1 (MQ=255) ccATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCt < 1:1890/251‑1 (MQ=255) agtggaagACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑TCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCCTGAAATTTGCGGGCGTCCGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACcgcg > 1:167330/1‑251 (MQ=255) gCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGgtcgtc < 2:440493/251‑1 (MQ=255) cGAAGGTATTGGCGGTTTTCCGATCGGCCCCCCGGACGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTTTATGTTTATGCGTCGCGGGTGCCGCGGGCATTACTGCTTCTTTTACCTCGGCGGCGCGGCGCATGAAATTGGGGTGCGTCAGGTGGGGCATTTTCTGTGGGAACGCTTTGGCAGCTCCCACCGCGGGGGTTTTGTCGCTCTTTATTTTCAACCGGGCGGCgggg > 2:403463/1‑251 (MQ=255) gTTTCCCGATCGGCACCCGTGAAGAAGTGCGGGCGCTCATTT‑CCGGTGCATTCGACTCCGGCGTTT‑ACAGTTATATGTTGATGAGACGCGGCTGCAGCGCGCATTACTGCTTCTTTAACCTCGGCGGCGAGGCGCATGAAATTGGCGTGCGTTAGGCGGAGCATTATTAGCGGAACCACTGTCACAGCGCCCACCCCGTGCGTAAAGTCGCTATTCATTTCGATGCGGTCGTCGGGGAAATTCTCGAGaa > 2:318553/1‑250 (MQ=255) | AAGCGTTGGTTCAGTATCGCGATCAGCTGGAAGGCAAAACCTTCTGCGTACGCGTGAAGCGCCGTGGCAAACATGATTTTAGCTCGATTGATGTGGAACGTTACGTCGGCGGCGGTTTAAATCAGCATATTGAATCCGCGCGCGTGAAGCTGACCAATCCGGATGTGACTGTCCATCTGGAAGTGGAAGACGATCGTCTCCTGCTGATTAAAGGCCGCTACGAAGGTATTGGCGGTTTCCCGATCGGCACCCAGGAAGATGTGCTGTCGCTCATTT‑CCGGTGGTTTCGACTCCGGTGTTT‑CCAGTTATATGTTGATGCGTCGCGGCTGCCGCGTGCATTACTGCTTCTTTAACCTCGGCGGCGCGGCGCATGAAATTGGCGTGCGTCAGGTGGCGCATTATCTGTGGAACCGCTTTGGCAGCTCCCACCGCGTGCGTTTTGTCGCTATTAATTTCGAACCGGTCGTCGGGGAAATTCTCGAGAA > NC_000913/441826‑442309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |