breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
MC JC 1,586,443 Δ555 bp [ydeP][ydeQ] [ydeP], [ydeQ]
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,695,565 C→T G169G (GGG→GGA uidA ← beta‑glucuronidase
RA 1,699,275 C→T intergenic (‑95/‑80) malI ← / → malX DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,823,288 C→T G161D (GGC→GAC)  recA ← DNA recombination/repair protein RecA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,880,635 T→C L138L (TTA→TTG casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,893,294 Δ1 bp coding (638/1272 nt) ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
MC JC 3,090,238 688 bp→74 bp [yggI][endA] [yggI], [endA]
MC JC 3,131,341 Δ128 bp IS5‑mediated yghQ ← putative transport protein YghQ
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,807,520 IS1 (–) +8 bp coding (572‑579/1035 nt) waaQ ← lipopolysaccharide core heptosyltransferase 3
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,585,480 G→A Q428* (CAA→TAA)  hsdR ← type I restriction enzyme EcoKI endonuclease subunit
RA 4,602,509 C→T W117* (TGG→TAG)  yjjP ← putative succinate exporter YjjP
RA 4,616,669 G→T L133I (CTA→ATA)  yjjI ← DUF3029 domain‑containing protein YjjI
MC JC 4,628,621 Δ6,226 bp ettA[rob] ettA, slt, trpR, yjjX, ytjC, [rob]
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258676 258682 7–775 23 [0] [0] 3 insB9–[crl] insB9,insA9,[crl]
* * ÷ NC_000913 275509 321802 46294 32 [0] [2] 3 [mmuP]–[ykgE] [mmuP],mmuM,afuC,insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,insF1,rclC,rclB,rclA,rclR,[ykgE]
* * ÷ NC_000913 321859 348973 27115 3 [1] [2] 3 [ykgE]–[prpB] [ykgE],ykgF,ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,[prpB]
* * ÷ NC_000913 348977 372744 23768 3 [2] [2] 4 [prpB]–[mhpD] [prpB],prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,lacY,lacZ,lacI,mhpR,mhpA,mhpB,mhpC,[mhpD]
* * ÷ NC_000913 1694762 1695469 708 4 [0] [0] 3 uidA beta‑glucuronidase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)23 (0.520) 22/460 0.7 100% intergenic (+8/+16) crl/insB9 RNA polymerase holoenzyme assembly factor Crl/IS1 family transposase B
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl RNA polymerase holoenzyme assembly factor Crl
* ? NC_000913 = 2755080 (0.000)32 (0.730) 30/456 0.4 98.5% coding (184/1404 nt) mmuP S‑methyl‑L‑methionine transporter
?NC_000913 372749 = 1 (0.020)coding (635/810 nt) mhpD 2‑hydroxypentadienoate hydratase
* ? NC_000913 1207790 =19 (0.410)12 (0.280) 12/444 1.4 43.9% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 13 (0.300)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780516 (0.350)23 (0.540) 22/444 0.7 62.2% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 13 (0.300)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase