Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	97.6%	120.4 / NA	41	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (24/16);  minor base A (1/0);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636684‑4637164                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                gAGGGGCCTGAAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGACTGGTTCGTCCTGGCCATTTTTGTCAAAGAAGTTTAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGGTCGACACCGCAAAGTTGCTGGCGGGGCTGGCGCGTCCCGATTTGCTCTCTGGGGGCCCCCCGGACTGGCCACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTcc                                                                                                                                                                                                                                        >  2:190208/1‑250 (MQ=255)     ggCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCACAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCACTCCGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTTATTAGCTACAACATCCCCCGTTTCGCGcc                                                                                                                                                                                                                                    >  2:302210/1‑251 (MQ=255)        cTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCCGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGGGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCGGGCGGGGCCCGGTTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGGGTTTAATCAGATCCAACATCCCCCGGTTCGCGCCAat                                                                                                                                                                                                                                 >  2:209421/1‑251 (MQ=255)           tAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACAACGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCCTGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATc                                                                                                                                                                                                                              >  1:273579/1‑251 (MQ=255)                     cGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGAGCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTa                                                                                                                                                                                                                    <  2:394898/251‑1 (MQ=255)                              gttgttGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCACCTACAACATCCCCCGTTTCGCGCCAATATCTATCGCATTAACAAACTgc                                                                                                                                                                                                           <  2:188318/251‑1 (MQ=255)                                 gttgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCa                                                                                                                                                                                                        <  1:142903/251‑1 (MQ=255)                                  ttgCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCCATATCGGTGGCCTTAACAAAGTGCGCa                                                                                                                                                                                                        >  2:173436/1‑250 (MQ=255)                                                      ggtggcggtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTatat                                                                                                                                                                                     <  1:97437/249‑1 (MQ=255)                                                             gtggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATTACCGATgc                                                                                                                                                                            <  2:445180/251‑1 (MQ=255)                                                               ggcAGCCTGGTTCGAACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATCTGACCGATgcgc                                                                                                                                                                          >  2:37465/1‑251 (MQ=255)                                                                            gTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCCATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTAtt                                                                                                                                                             >  2:411876/1‑251 (MQ=255)                                                                                   ccATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAAGGAGGGGAAGTTGATCGACACCGCAACGTTGCTGGCGGAGCTCGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCCACTGGCGCAGGCGTTTAATCAGCTACAAAATCCCCCGTTTCGCGCCAATCTCGGTGGCCTTAACAAAGCGCGCAATGAATATCATGTCTATATGACCGATGCGCCGGGCAACGTATTTTTCgct                                                                                                                                                      >  2:428954/1‑249 (MQ=255)                                                                                       ttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCg                                                                                                                                                   <  2:273579/250‑1 (MQ=255)                                                                                             cAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAAt                                                                                                                                             <  2:177332/250‑1 (MQ=255)                                                                                               aagaagTTAAACCGGGCGTGCGAAGAGCAACGCAGGGGACGTTGGTCGACACCGCAACGTTGCCGGCGGAGCAGGCGCGTCCCGCTTTGCTCTCCGGGGACCCAACCCATGGGCAACTGGCGCCGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGAGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCCGGGCAAAGTATTGTTAGATTAGGCAAATaa                                                                                                                                           >  2:51837/1‑250 (MQ=255)                                                                                                        aaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGGGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGAATTGTTCGATTCGGCAAATAAAGCCGTTgg                                                                                                                                  >  2:100328/1‑250 (MQ=255)                                                                                                        aaCCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGGCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGa                                                                                                                                 <  2:314813/251‑1 (MQ=255)                                                                                                          ccGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGGATTGTTCGATTCGGCAAATAAAGCCGTTGGAc                                                                                                                                >  1:134400/1‑250 (MQ=255)                                                                                                                  gCGAAGAGCGACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGAGGAGCTGGCGCGTCCCGCTTTGCCCTCTGGGGACCCACCGCATGGGCAACTGGCGCAGGCGTTTAATACGCTACCACATCCCCCGTTCCCCGCCAATCTCGGTGGAATTCACAAAGCGCGCACTGAATATCCAGCCTCTATGACCCATGCGCCGGGCCAAGGATGGTTCTATTCGGCAAATAAAGCCGTAGGCCAGGattatt                                                                                                                       >  2:223752/1‑251 (MQ=255)                                                                                                                     aaGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTcgc                                                                                                                    >  1:164702/1‑251 (MQ=255)                                                                                                                                      cGTTGATCGACACCGCAACGTTGCTGGCGGGGCTGGAGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAACTAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTgg                                                                                                   >  2:173381/1‑251 (MQ=255)                                                                                                                                       gTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGc                                                                                                  >  1:90369/1‑251 (MQ=255)                                                                                                                                          gATCGACACCGCAACGTGGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTAGAATGACGTCTGGCTa                                                                                                <  2:9494/250‑1 (MQ=255)                                                                                                                                             cGACACCGCAAAGTTCCTGGCCGAGCTGGCGCGCCCCGATTTGATCCCTGGGGACCCGCAGCCTGAGAAACCGGCGCAGGCGTTTAATCAGCTACAACATCAACCGTTTCGCCCCAATATCGGTGGCATTTAAGAAGTGCGTCATGAATAACATGTCTATATGCCAGATGCGCCGGGCCAAGTATTGTTCGATTCGGACAATATAGCCGTTGGCCAGGCTTATTCGCGCTGGACAGACGTCTGGATAACGt                                                                                            >  2:194907/1‑251 (MQ=255)                                                                                                                                               acacCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTg                                                                                          >  1:171849/1‑251 (MQ=255)                                                                                                                                                               gtcGGAGCTGGCGCGTTCCGATTTTCTCTCTGGAGACCCAACGCATGGGCAACTTGCGCTGTCTTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGCCCGATGCGCAGGGCAAAGTTTTGTTCGATTCGGCAAATAAAGCCGTTTGACAGGATTATTCGCGCTGGAATGACGTCTGGCTATCGTTTCGGGGTTAGTATGGTg                                                                          <  2:376756/249‑1 (MQ=255)                                                                                                                                                                 cGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTGAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGCCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTTGTCAGTATGGTgcg                                                                        <  2:283117/251‑1 (MQ=255)                                                                                                                                                                  ggAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGGTTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAAGATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcgc                                                                       <  2:188803/251‑1 (MQ=255)                                                                                                                                                                    aGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAGCTGGCGCAGGCGTTTAATCCGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAACGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGGgcgcgc                                                                     >  2:49008/1‑251 (MQ=255)                                                                                                                                                                     gCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATCTCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGGATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTgcgcgc                                                                     >  2:113388/1‑250 (MQ=255)                                                                                                                                                                             gTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGAGCAGCACGTTg                                                            >  2:162976/1‑251 (MQ=255)                                                                                                                                                                               cccGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGc                                                           >  1:419174/1‑250 (MQ=255)                                                                                                                                                                                  gATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGCTGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATCAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGaaaa                                                        >  2:436145/1‑250 (MQ=255)                                                                                                                                                                                                  cctcAACGCATGGGCAACTGGCGCAGGTGTTTAATCAGTTTCAACATCCCTCGTTGCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGTCCGATGCGCAGGGCAAAGTATTGTGCGATTCGGCAAATAAAGCCGTTGGACTTGATTATTGGCTCTGGAATGACGGCTGGCTAGCGTTGCGTGGTGAGTATGTTGCTCGCATCACGTTGTAAAATCCTGCCGATCCCGaa                                       <  2:276102/248‑1 (MQ=255)                                                                                                                                                                                                                 aaCTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtat                        <  1:173436/251‑1 (MQ=255)                                                                                                                                                                                                                  aCTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCACTATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGCTGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtatg                       >  1:50837/1‑251 (MQ=255)                                                                                                                                                                                                                      gcgcAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGGCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAAGCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGc                   <  2:264705/251‑1 (MQ=255)                                                                                                                                                                                                                                ttAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGAGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGAGCAGAACGTTGCAAAATCCTGCCGATCACGAAAGTTCTGTGATGTATGTTGCCGCACCGAtt         >  2:305137/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaTCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTAt       >  1:303020/1‑251 (MQ=255)                                                                                                                                                                                                                                       gCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACg  <  2:171849/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                GAGGGGCCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCGATTATGGACG  >  NC_000913/4636684‑4637164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑