Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,699,275	C→T	intergenic (‑95/‑80)	malI ← / → malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,699,275	0	C	T	100.0%	72.8 / NA	23	intergenic (‑95/‑80)	malI/malX	DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/13);  total (10/13)

TTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCT  >  NC_000913/1699043‑1699515                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 gtCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGACATGCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTAGTTTTTTGGCGGGAGCCATGCAGATGACCTACTCCCTTTTTATGTGACAGATAAAACTTGTTACCTTTTATTTATCGTATACCCGCTATTATCGTGGCGTAATGTGATTTATGTCTCGCTAACATGTGATaa                                                                                                                                                                                                                                <  2:290001/250‑1 (MQ=255)                   gcgTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGGAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGGTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGGTTATGTGACAGATAAAACGGTTTACCTTTTATTTATCTTTTAACCGCTATTATCGTTGCGGAATGTGATTTATGTCTCACTAAAATTTGATAAAAAGGTTTAtctttttgc                                                                                                                                                                                                              >  1:50685/1‑246 (MQ=255)                                                      cACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTGTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTGCCTTTTATTTATCTTATACCCGCTATTATCTTTGCGTAATTTGATTTATGTCTCAGTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACgagga                                                                                                                                                                           <  1:424546/251‑1 (MQ=255)                                                       ccTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCTCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTGTTATCTTCTCGCGCAATTTACTGAATTCAGATTGTTCTCTACgaggat                                                                                                                                                                          <  2:29894/250‑2 (MQ=255)                                                                                  cGACACGCCCGCAGCCAGCGCAACATCATGAATGGCTATTCTTTAGGCGGTAGCCATGCAGATGACCTACTCACTGCTTATGTGACCGATAAAACGTTTTACCTTTTATTTATCTTCTACCCGCTATTATCGTTGCGAAATGTGATTTATGTCTCACTAAACTTTGCCAAAACGTTTTATCTTCCCGCGCAATTTACTGCCTCCCGATTGTTCTCTACGCGGAGGCGTTTTATGACGGCGAAAACAGcccc                                                                                                                                               >  2:33598/1‑248 (MQ=255)                                                                                  cGACACCCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGAAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCAcc                                                                                                                                               >  2:143108/1‑251 (MQ=255)                                                                                                                                   gTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTATCACTAAAATTTGATAAAACGTTTTAtc                                                                                                                                                                                                                     >  1:224581/1‑132 (MQ=255)                                                                                                                                   gTAGCCATGCAGATGACCTACTCCCTGATTATATGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCGCACTAAAATTTGAGAAAACGTTTTAtc                                                                                                                                                                                                                     <  2:224581/132‑1 (MQ=255)                                                                                                                                     aGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGtt                                                                                            >  1:322894/1‑251 (MQ=255)                                                                                                                                         aTGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGt                                                                                                                                         <  1:173308/202‑1 (MQ=255)                                                                                                                                         aTGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGt                                                                                                                                         >  2:173308/1‑202 (MQ=255)                                                                                                                                           gCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCg                                                                                       <  1:359229/250‑1 (MQ=255)                                                                                                                                                     tACTCCCTGAGTATGTGATAGATAATACGTTTTCCCTTTTATTTATCTTGTACCCGCTACTATCGTTGCGTAATGTGATTTATGTCACACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCATATTGTTCTCTACGAGGAGTTTTTTTATGACAGCGAAAACAGCACCGATAGTCACGCTGGTGGAGTGTTTCCAGCAGTTAGGCAAAACCTTCATGTTGCTCGTGGGattatt                                                                             <  2:118523/250‑1 (MQ=255)                                                                                                                                                      aCTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACTATGAGTCTTTTTATGACGGCGAAAACAGCTCCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTg                                                                            <  1:460753/250‑1 (MQ=255)                                                                                                                                                         cccTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCg                                                                         <  1:162086/250‑1 (MQ=255)                                                                                                                                                             gATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTc                                                                      <  2:210619/249‑1 (MQ=255)                                                                                                                                                                         gATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCt                                                        <  2:368825/251‑1 (MQ=255)                                                                                                                                                                          aTAAAACGTTTTACCTTTTATTTATCTTATCCCCGCTATTATCGTTGCGTAATTTGATTTCTGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCCTTATTGTCGTTCTGCGGCATTATgatc                                                       >  2:282072/1‑248 (MQ=255)                                                                                                                                                                            caatcgttaaaccgtttatttaTGTAATACCCGCTATTATAGTCGAGTCATGTGACTTATGTATCACTAAAATCTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAACCAGCACCGAAAGTCACGCTGTGGGAGTTCTTACAGCAGTTAGGCAAACCATTCATGTTACCAGTGGAATTATTGTCGTTCTGAGGCATTATGCTCgg                                                     >  2:468823/15‑251 (MQ=255)                                                                                                                                                                                   tttACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCTTTGGt                                              >  2:468124/1‑251 (MQ=255)                                                                                                                                                                                                        aCCCGCTATTATCGTTGCGTAATGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCatga                          <  2:322894/250‑1 (MQ=255)                                                                                                                                                                                                                             aTGTGATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTc    >  1:220414/1‑251 (MQ=255)                                                                                                                                                                                                                               gtgATTTATGTCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCt  <  1:468124/251‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                 TTCAATGGCGGCGTTAACGCGTTCTCCTGTGGCGGTAGAGATTCGCCCTTTGCCACTAAGCACCAGCGAAACGGTACTTACCGACACGCCCGCAGCCAGCGCAACATCATGAATGGTTATTTTTTTGGCGGTAGCCATGCAGATGACCTACTCCCTGATTATGTGACAGATAAAACGTTTTACCTTTTATTTATCTTATACCCGCTATTATCGTTGCGTAATGTGATTTATGCCTCACTAAAATTTGATAAAACGTTTTATCTTCTCGCGCAATTTACTGAATCCAGATTGTTCTCTACGAGGAGTCGTTTTATGACGGCGAAAACAGCACCGAAAGTCACGCTGTGGGAGTTCTTCCAGCAGTTAGGCAAAACCTTCATGTTACCCGTGGCATTATTGTCGTTCTGCGGCATTATGCTCGGCATTGGTAGTTCTCTTAGCAGCCATGATGTCATAACCCTGATCCCGGTCCT  >  NC_000913/1699043‑1699515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑