breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 74 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,212,9590GC79.5% 90.1 / 20.1 40intergenic (+13/‑81)rrlE/rrfE23S ribosomal RNA/5S ribosomal RNA
*NC_0009131,336,6740GA78.7% 235.6 / 53.2 108G282R (GGG→AGG) acnAaconitate hydratase 1
*NC_0009134,449,5860GA77.3% 191.5 / 51.6 88P23S (CCG→TCG) ppainorganic pyrophosphatase
*NC_0009131,001,2510AC76.3% 170.3 / 35.1 76Q679P (CAA→CCA) elfCputative fimbrial usher protein ElfC
*NC_0009132,042,1710GA76.0% 258.5 / 81.4 129intergenic (+146/‑197)zinT/yodBmetal‑binding protein ZinT/putative cytochrome b561 YodB
*NC_0009134,423,3370CT75.7% 140.3 / 41.9 70S164S (TCC→TCTulaEL‑ribulose‑5‑phosphate 3‑epimerase UlaE
*NC_000913696,4700CT75.3% 167.7 / 52.8 85noncoding (35/75 nt)glnXtRNA‑Gln
*NC_0009133,152,4940GT75.3% 163.6 / 63.6 97A87S (GCG→TCG) metCcystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase
*NC_0009131,287,0400GA73.2% 95.8 / 28.9 58intergenic (+56/+26)ychS/rttRputative uncharacterized protein YchS/small RNA RttR
*NC_0009132,142,1500AG70.1% 108.0 / 67.6 77R22R (CGT→CGCdcddCTP deaminase
*NC_00091358,0650CA38.7% 127.7 / 22.8 93P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009131,351,4630CA37.8% 86.9 / 24.6 90E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009134,213,3100GT37.4% 167.5 / 14.2 107G26V (GGA→GTA) yjaAstress response protein
*NC_0009132,155,8800CT33.9% 60.4 / 58.2 59L206L (CTC→CTTmdtBmultidrug efflux pump RND permease subunit MdtB
*NC_0009133,669,7150GT31.1% 174.5 / 10.0 104E42* (GAG→TAG) treFcytoplasmic trehalase
*NC_0009133,911,5330CA30.6% 276.2 / 17.4 144intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,990,4800CA30.1% 239.3 / 10.3 133intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009131,466,4380TG29.6% 74.2 / 26.3 54pseudogene (1047/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009131,936,6340CT29.6% 207.4 / 115.7 136intergenic (‑320/‑18)zwf/yebKNADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK
*NC_0009132,472,8170CA29.1% 156.0 / 12.0 86G22V (GGA→GTA) yfdNuncharacterized protein YfdN

Marginal new junction evidence (lowest skew 10 of 46 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 69674158 (0.620)15 (0.220) 12/432 3.5 21.2% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 69 (1.010)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 69646966 (0.710)25 (0.270)
+T
18/586 3.5 27.1% noncoding (36/75 nt) glnX tRNA‑Gln
?NC_000913 696583 = 69 (0.740)noncoding (34/75 nt) glnV tRNA‑Gln
* ? NC_000913 = 4542690163 (1.750)22 (0.240) 17/570 3.6 13.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 132 (1.460)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 3583428 =NA (NA)19 (0.200) 18/588 3.6 17.0% noncoding (1/768 nt) IS1 repeat region
?NC_000913 3731420 = 93 (1.000)coding (290/993 nt) xylF xylose ABC transporter periplasmic binding protein
* ? NC_000913 = 3584195NA (NA)18 (0.190) 16/588 4.0 17.3% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 3731428 86 (0.920)coding (298/993 nt) xylF xylose ABC transporter periplasmic binding protein
* ? NC_000913 = 19785021 (0.010)20 (0.220) 15/572 4.1 97.6% intergenic (‑305/+16) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA5/uspC IS1 family protein InsA/universal stress protein C
* ? NC_000913 2765412 =115 (1.230)18 (0.230) 11/500 4.5 15.6% intergenic (‑259/+101) abpA/yfjM anti‑bacteriophage protein/protein YfjM
?NC_000913 = 2765419 97 (1.220)intergenic (‑266/+94) abpA/yfjM anti‑bacteriophage protein/protein YfjM
* ? NC_000913 2288919 =NA (NA)17 (0.180) 13/586 4.7 14.8% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3349205 98 (1.060)coding (605/729 nt) mtgA peptidoglycan glycosyltransferase MtgA
* ? NC_000913 = 2237687102 (1.090)17 (0.180) 13/588 4.7 14.3% coding (67/1011 nt) mglC D‑galactose/methyl‑galactoside ABC transporter membrane subunit
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 4542682 =150 (1.610)18 (0.200) 12/570 4.8 11.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 135 (1.490)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit