breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,477,2650AC38.3% 104.1 / 31.9 81L122* (TTA→TGA) ‡dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009134,136,4670AC37.8% 49.2 / 12.2 45E120D (GAA→GACyijEcystine exporter
*NC_000913161,5590GA36.8% 95.5 / 12.8 58S158L (TCG→TTG) thpRRNA 2',3'‑cyclic phosphodiesterase
*NC_0009131,922,8150CA32.6% 152.6 / 16.6 92G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009133,054,8940GT32.3% 141.8 / 25.8 96P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009131,097,1180CA32.3% 20.2 / 23.1 31intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009133,269,9430GT30.9% 63.7 / 14.6 56intergenic (+341/+273)yhaC/rnpBuncharacterized protein YhaC/RNase P catalytic RNA component
*NC_000913847,9550GA30.5% 169.8 / 30.5 106A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009134,249,8100CA29.5% 173.9 / 14.7 88P86Q (CCG→CAG) malMmaltose regulon periplasmic protein
*NC_0009133,054,8860GT29.0% 156.6 / 26.4 93Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009134,296,2680TC29.0% 45.3 / 15.0 31intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,923,5390CA28.3% 191.2 / 13.9 99E48* (GAG→TAG) rsmG16S rRNA m(7)G527 methyltransferase
*NC_000913782,1940TG28.2% 182.5 / 14.7 103L37R (CTA→CGA) nadAquinolinate synthase
*NC_0009132,239,9940GT27.3% 247.3 / 16.4 110R119S (CGT→AGT) mglBD‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
*NC_0009131,334,9660AC27.1% 204.0 / 25.1 124intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_000913538,0750AG26.6% 135.4 / 12.1 64D148G (GAT→GGT) ybbWputative allantoin transporter
*NC_0009132,767,4450CA26.5% 197.3 / 10.3 84intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009133,202,0040CA26.3% 146.1 / 55.2 76G38G (GGC→GGAccafused CCA tRNA nucleotidyltransferase/phosphohydrolase
*NC_0009132,477,2640TC25.9% 162.5 / 13.9 81L122L (TTA→TTG) ‡dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009133,226,2240GT25.6% 260.4 / 10.1 125intergenic (+53/‑10)ebgC/ygjIDUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta/putative transporter YgjI

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2357730103 (1.150)10 (0.130) 10/518 5.0 10.0% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 90 (1.160)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 460616690 (1.010)5 (0.080) 5/420 6.0 7.9% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 53 (0.850)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 265341976 (0.850)7 (0.090) 7/548 6.4 9.1% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 71 (0.870)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 265350291 (1.020)6 (0.080) 6/530 6.7 7.5% intergenic (‑47/‑353) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
?NC_000913 2653825 = 67 (0.850)intergenic (‑370/‑30) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 78122244 (0.490)4 (0.060) 4/446 6.8 10.8% noncoding (76/76 nt) lysY tRNA‑Lys
?NC_000913 781653 = 33 (0.500)intergenic (+1/‑432) lysQ/nadA tRNA‑Lys/quinolinate synthase
* ? NC_000913 = 4542690158 (1.770)6 (0.070) 6/580 7.2 4.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 111 (1.280)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =155 (1.740)6 (0.070) 6/580 7.2 4.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 111 (1.280)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 3469405116 (1.300)4 (0.050) 4/566 8.1 3.0% coding (449/2694 nt) chiA endochitinase
?NC_000913 3469715 = 151 (1.790)coding (139/2694 nt) chiA endochitinase
* ? NC_000913 = 125176672 (0.810)4 (0.050) 4/574 8.1 5.6% coding (701/1920 nt) dhaR DNA‑binding transcriptional dual regulator DhaR
?NC_000913 1252103 = 65 (0.760)coding (1038/1920 nt) dhaR DNA‑binding transcriptional dual regulator DhaR
* ? NC_000913 = 8208262 (0.020)3 (0.030) 3/592 8.9 60.2% coding (34/714 nt) ybhM Bax1‑I family protein YbhM
?NC_000913 2066159 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region