| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 161,559 | 0 | G | A | 36.8% | 95.5 / 12.8 | 58 | S158L (TCG→TTG) | thpR | RNA 2',3'‑cyclic phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (7/29); new base A (21/0); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.55e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGA > NC_000913/161261‑161851 | cgccgcTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCANCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACga < 2:626848/300‑1 (MQ=255) gcTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGTGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACgaggag < 1:638174/300‑1 (MQ=255) aTTCCCATGTGTGTTGCTATTTCCCTTTGGTGTTGTCTGAATTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAACTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGTTG‑AACTc < 2:201222/300‑1 (MQ=255) aTTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAGTGTGCGTCCACGGG‑AAAAAAAGGAGGAGTCAAAggggaaccc > 1:681679/1‑294 (MQ=255) gtgtttCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAc < 1:226023/297‑1 (MQ=255) gTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCATGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACATTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAc < 2:150347/300‑1 (MQ=255) gTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAc < 1:55962/300‑1 (MQ=255) gTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTTTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGTAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTg < 2:248288/300‑1 (MQ=255) gTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTGGGGTGTGTGTCCCCAGG‑CAAACAAGGAGGAGT‑AAAGGGTG‑AACTCCGTCCCCGCATACGAA‑C‑AGTT‑AAAAcccggaggaggggttg > 1:707851/1‑286 (MQ=255) cGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGTTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCAGCGGGTGTGATCACATCGGCTAAAAAACGTTNGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTNTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTc < 2:559711/300‑1 (MQ=255) ttGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGAGTTTTCGTCCCCAGG‑AAAAAGAAGAGGAGT‑CAAAGGTG‑GACCCCATCACCCCCTACAAC‑C‑AATT‑CAAACCTGGCTGCGGG‑GTTGTC‑CCCGCCCCTCTTGCGTcgcttaataa > 1:401659/1‑293 (MQ=255) cacaACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGt < 1:269730/300‑1 (MQ=255) caACCGGTCATCTCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTaa < 2:404186/300‑1 (MQ=255) tCGCACCCGTATTCGTGCAGTTTAGCGTTAATGCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCGTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATg < 1:165423/300‑1 (MQ=255) tCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGTGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATg < 1:193666/300‑1 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCTGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCCAGG‑AAAACAAGGAGGCGT‑CAAAGGTG‑GACCCCATCCCCACATACGAA‑C‑AATT‑CAAACCCGGCGGAGGG‑GGTTTT‑ACCAACCCGCTGGAGTCGCGCAATAAGGGAATATGTGGGTGAAAAAGACAATTGCttttc > 1:223034/1‑298 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTCGCGGGTGCGTCCCCAGG‑CCAACAAGGAGGCGG‑AAAGGGTGGAAC‑CCATCCCCGAACACGAC‑C‑AGTT‑AAAAACTGGCGGAGGG‑GTTTTC‑CCCACCCCTCCGGCGTCTAACAATAAGGTAATACGTGGACGAAAcagaagaacactttgc > 1:404703/1‑285 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACAGG‑AAAACGAGGAGGAGT‑AAAAGGTG‑AACCCCGTCACCACACACGAC‑C‑AATT‑CAAACCCGGCGGCGGG‑GTTGTC‑CCCACCTCGCTGGCGTCGAGCAATAAGGTAATATGTGGATGAAAAGGAAGATTGCtttta > 1:403875/1‑298 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGAATGTGTGTCCACAGG‑AAAACAAAGAGGCGT‑GAAGGGTG‑GAATCCGTCACCGCACACGAA‑C‑AGTT‑GAAACCTTGCGGGGGG‑GTTGTC‑ACCACCTCGCCGGCATTGAGCAACAAGGGAATCGGTGGATGAAAAGGAAGATTGCTTT‑Ga > 2:408762/1‑300 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAACGTGTTTTCCCACAGG‑CAAACAAGGAGGCGT‑AAAAGGTG‑AACCCCCTCCCCCTCTCCGAA‑C‑CGTT‑CAAAACTGGCGGCGGG‑GTTGTC‑ACCGCCTCGCTGGTGTCACGCAATAAGGTCATATGTGGATTAAACGGACGACTTCTTT‑Ga > 2:26396/1‑300 (MQ=255) gtgtAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGGATGTGCGTCCCCAGG‑CAAAAAAGGAGGCGT‑AAAGGGTG‑GACCCCATCACCACACCCGAA‑C‑AATT‑CAAACCCTGAGGAGGG‑ATTGTCCACC‑CCCCACTTGTGTTTCGCAATAAGGGAATCTGTGGATTAAACGGACGATTGCtttta > 1:127607/1‑298 (MQ=255) gCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCCCGGG‑CAAACAAGGAGGAGT‑CAAAGGTG‑AACTCCACCCCCACACACGAA‑C‑AATT‑CAAAACTTGCGGCGGG‑AGTTTT‑ACCACCTCGCTGGAGTTGCGCAACAAGGTAAGATGTGGATGAAAAGGAAGATTGCTTT‑TAAaaca > 1:453176/1‑300 (MQ=255) aTTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGCGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGGGGGTGTGTCCCCGGGACAAA‑GAGGGGGGGT‑GAAAGGTG‑AACCCCGTCACCGCAGACGAAAC‑ATTT‑ACAACC‑GGCGGCGGGGATT‑TC‑ACCACCCCTCCGGGGTCGCGAAAGAAGGGGACATGTGGGTGAAACGGACAAGTGTTTT‑GGAAACAAccacc > 1:531950/1‑297 (MQ=255) aTTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAAAGTGTGTGTCCACGGG‑AAAAAAAGGGGGCGG‑AAAGGGTG‑AACCCCGTCACCTCACACGAA‑C‑AATT‑GAAACCTGGCGGGGGG‑GTTGTT‑AACACCTCGCTGGTGTTGCGACATAAGGTAACATGTGGATTACACGGAAAATTGCTTT‑TAAAACAAccccc > 1:532362/1‑297 (MQ=255) cATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGTGTCCCCAGG‑CAAACAAGGAGGAGT‑AAAAGGTG‑AACCCCATCCCCACACCCCAC‑C‑AATT‑CAAACCCTGCGGAGGG‑GTTGTC‑CCCGCCCCACTTGCGTTGCGCAATAAGGTAATATGTGGATGAAAAGGACAATTTTTTT‑TAAAAAAAACACTGCGGGC‑Aga > 1:433719/1‑299 (MQ=255) ggTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCCAGG‑AAAACAAAGAAGAGT‑AAAGGGGG‑GAATCCTTCACCGCATCCAAC‑CCATTC‑AAAACC‑CGCGGAGGG‑ATTTTT‑CCCGCCCCGCTTGCGTTGAGCAAGAAGGTCATGTGTGGGTGGAACAGACAACTTTTTT‑GAAAACAACCACTGCGGGC‑AGCCtgtt > 1:215652/1‑299 (MQ=255) ggTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGAGTGTGTGTCCCCGGG‑CAAAAAAGGAGGAGT‑AAAGGGTG‑AACCCCATCACCACACACGAA‑C‑CGGT‑AAAACCCTGCGGAGGG‑GTTGTC‑CCCACCCCGCTTGTGTTGCGCAAGACGGTAATATGTGGATGCAACGGATGATTGCTTT‑TAAAAAACCCCCTGCGGGC‑AGCCtgtg > 1:584767/1‑300 (MQ=255) ggTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGCGTGTGCGTCCACAAG‑AAAAAAAGGAGGAGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑CAAACCTGGCGGCGGG‑GGTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTCNTATGTGGATGAAACGGACGATTGCTTT‑GGAAACAACCGCTGCGGGC‑ANCCtgtg > 2:590432/1‑300 (MQ=255) tgtgATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGaa < 1:284072/300‑1 (MQ=255) gtgATCACATCTGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAAc < 1:125809/300‑1 (MQ=255) gtgATCACATCGGCTAAAAAACGGTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAAc < 2:436981/300‑1 (MQ=255) gtgAGCACAGCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTTTAGAGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGTCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAAc < 2:81276/300‑1 (MQ=255) cGGCTANAAAACGTTGGGAACGCTGAAGTAGCGTCGCGCGCTGTAGAGGGGGAGNAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATAtt < 2:577692/300‑1 (MQ=255) cGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATAtt < 1:34091/300‑1 (MQ=255) gCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCg < 2:417560/300‑1 (MQ=255) ttGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCCAGG‑AAAACAAGGAGGAGT‑AAAAGGTG‑AACTCCATCCCCACACACAAA‑C‑AATTCAAAACC‑CGCGGGGGG‑ATTGTC‑ACCACCCCGCTTGCGTCGCGCAACCAGGTAATATGTGGATGAAACGGACGATTGCTTT‑TAAAACAACCGCTTCGGGC‑CGCCTTTGAACGGAGCaccttttacagcctgattc > 1:246408/1‑282 (MQ=255) gcgcgcTGTAGAGGGGTAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCa < 1:382649/300‑1 (MQ=255) gcgcgcTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCa < 1:707508/300‑1 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACANTCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGCGTGTGTGTCCCCGGG‑CAAACAAGGGGGGGT‑AAAAGGTG‑GACTCCATCCNCACATACGAA‑C‑AGTTCAAAACCTG‑TGGATGG‑ATTTTC‑CCCGCCTCGCTGGCGTCTCGCAACAAGGTAATATGTGGATGAAAAAGACGATTGATTT‑TAAAACAACCGCTGCGGGC‑AAACTGTGAACGGAGCATATTCGCCAACCTGATTCAGCCGCATGGCGGCTGACGCATcccc > 2:500277/1‑299 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTTTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCt < 2:342312/300‑1 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGGGTGTTCTTCCCCGGG‑AAAACAAGGAGGAGT‑CAAAGGTG‑AACCCCGTCCCCACATCCAAA‑C‑AATT‑CAAACCCGGCGGCGGG‑ATTGTC‑CCCACCCCACCGGTGTCGAGCAATAAGGTAACATGTGGATGAAACAAACGATTGCTTT‑TAAAACAACCGCCGTGGGC‑AGCCCGTGGACGGAGCATCTTCTCCCACTGGATTCAGACGCGTGGCGGTTGACGCATCCCt > 1:622503/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTTCGTCCCCAGG‑CAAACAAGGAGGAGT‑AAAAGGTG‑AACTCCATCCCCGCATACGAC‑C‑AGTT‑CAAACCTGGCGGAGGG‑ATTGTT‑ACCGCCTCGCTGGCGTTGAGCAACAAGGTAATATGTGGATGAAACGGACAATTTCTTT‑TAAAACAACCGCTTCGGGC‑AGCCTGTGAACGGAGCACCTTCTCCAACTTGATTCAGCCGCGTTGCGGCttacacaccccc > 1:693135/1‑289 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCCAGG‑CAAACAAGGAGGCGT‑AAAAGGTG‑AACTCCATCCACGCATACGAA‑C‑AATTCAAAACC‑GGCGGCGGG‑ATTGTC‑ACCACCTCGCTGGTGTCGCGACATAAGGTAATACGTGGATGAAACAGACGATTGCTTT‑TAAAAAAACCACTGCGGGCCACCCT‑TGAACAGAGCATCTTTTCCCACTTGATTCAGCCACGTGGTGGCTGACGAATCCCt > 1:569027/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGAGTGTGCGTCCACGGG‑AAAACAAGAAAGAGT‑AAAAGGTG‑AACCCCGTCACCACATCCGAA‑C‑AGTT‑CAAACCCGGCGGCGGG‑AGTGTT‑ACCGCCTCGCTGGCGTCGCGCAATAAGGCGATATGTGGATGAAACGGAAGATTGCTTT‑GGAAACAACCGCTGCGGGC‑AGCATTTGGAGGGAGGATATTCGCCAGCTGGATTCAGCCGCGTGGTGGGCGATGCATCCCt > 1:150835/1‑300 (MQ=255) cTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGAGAGTGTGTGTCCCCGGG‑AAAAAAAGGAGGAGT‑AAAAGGTG‑AACCCCATCACCACACCCGAA‑C‑CGTT‑CAAACCTGGCGGAGGG‑GTTGTC‑ACCACCTCACCGGGGTTGCATAATAAGGTAACATGTGGATTAAACAGACAATTGGTTT‑TAAAACAACCGCCGCGGGT‑AGCTCGTGGAAGGAGCATATTCGCCAGATTGATTCAGCCGCATGGCGGCTTGCGCATcccc > 1:225738/1‑299 (MQ=255) gTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCCCAGG‑CAAACAAGGGAGCGT‑AAAAGGTG‑AACTCCATCACCACATCCAAA‑C‑AATT‑AAAAACTCGCGGAGGG‑ATTGTT‑ACCACCCCTCTGGCGTCGCGAAACAAGGGAATATGTGGATGAAAAAGACGATTGTTTT‑TAAAAAAAACACCGTGGGC‑AGAATGTGGACAGAGAATATTTGCCAGCTGGAGTCCGCCGCGTGGTGGCTGACGCAcccccca > 1:195139/1‑297 (MQ=255) agagtgggagggaaTTTCATTTCGACAGTCTTTGTTATTACTTTAGCGCCCGGCNTTGTAGCTGCGTGTAGCATATGCGTCCACGGT‑CAAACTAGTAGGCGT‑AAAGGGTG‑AACTCCGTCACCTCATACGAC‑C‑AATT‑AAAACCTGTCGGCGGG‑ATTGGC‑ACCGCCTCACTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAacc < 2:517079/288‑1 (MQ=255) ggggAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCGTATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAaccacac < 1:299431/300‑1 (MQ=255) ggggAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAaccacac < 1:299427/300‑1 (MQ=255) cAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGNCGCGCAATAAGGTAATATGTGGATGANACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTg < 2:488876/300‑1 (MQ=255) ttGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGGGGCGTGTGTGTTTCCGGG‑CAAACGAAGGGGGGT‑GAAGGGGG‑AACTCCGTTCCAGCATACGAC‑C‑AGTT‑CAAACCCTGTGGGGGG‑ATTTTC‑CCCCCCTCCCTGGCGTCGCGCAAAGAGGTAAGACGGGGATNAAACGGACGATTGCTTTTGAAAA‑AACCCCCTCGGGC‑AACCCATGGACGGAGGCTATTTTCCAGCCGGGTTCAGCCGCACGGCGGCTGACGCATCCCTCCCCCCACCACACCCGAACGCAACCATTGCCCgat > 2:172468/1‑298 (MQ=255) tGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGtcatca < 2:221776/300‑1 (MQ=255) ttAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCg < 1:449028/300‑1 (MQ=255) ttttAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAAcc < 2:447451/300‑1 (MQ=255) ttttAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAAcc < 1:582701/300‑1 (MQ=255) tAGCGGCGTGTAGCGTATGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACTAgg < 2:241002/300‑1 (MQ=255) cgTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACg < 1:384311/300‑1 (MQ=255) cgTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCAAACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACg > 1:696659/1‑300 (MQ=255) cgTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑AACGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCAAACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACg > 1:696464/1‑300 (MQ=255) gg‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGNGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAgaga > 1:493166/1‑300 (MQ=255) | CGCCGCTCTGGCTTTGAGTTAATTCCCAGGTGTGTGGGTATTTCCGTTTGGTGTTGTCTGAAGTCGAATACCAGACGGTATCGCCAGGCGTTGCACAACCGGTCATCGCACCCGTATTCGGGCAGTGTAGCGTTAATTCGCGACCATCGGGTGTGATCACATCGGCTAAAAAACGTTTGTAACGCTGAATTAGCGTCGCGCGCTGTAGAGGGGGAGAAAATTCCATTGCGACAATCCTTGTTATTGCGTTAGCGCCCAGCGTTTTAGCGGCGTGTAGCGTGTGCGTCCACGGG‑CAAACGAGGAGGCGT‑AAAGGGTG‑AACTCCGTCACCGCATACGAC‑C‑AGTT‑AAAACCTGGCGGCGGG‑ATTGTC‑ACCGCCTCGCTGGCGTCGCGCAATAAGGTAATATGTGGATGAAACGGACGATTGCTTT‑GAAAACAACCGCTGCGGGC‑AGCCTGTGAACGGAGCATATTCGCCAGCTGGATTAAGCCGCGTGGCGGCTGACGCATCCCTAACCACACCACACGCGAACGCAGCCATTGTCCGGCGTCATCAAGCGTGAGTGTGAAACCAGGTTGACGAATCCGTCCGGCTAAAAGAGA > NC_000913/161261‑161851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |