breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 83,579 (A)7→8 intergenic (‑50/+43) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 124,078 C→T D354D (GAC→GAT aceE → pyruvate dehydrogenase E1 component
RA 137,378 C→T T99M (ACG→ATG)  cueO → multicopper oxidase CueO
RA 198,747 A→G S274G (AGC→GGC)  bamA → outer membrane protein assembly factor BamA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 262,762 Δ1 bp intergenic (+6/‑109) proA → / → thrW glutamate‑5‑semialdehyde dehydrogenase/tRNA‑Thr
RA 498,116 C→T P21L (CCT→CTT)  hemH → ferrochelatase
RA 498,809 A→G K252R (AAA→AGA)  hemH → ferrochelatase
RA 499,060 T→G D305A (GAC→GCC)  aes ← acetylesterase
MC JC 499,082 Δ52,248 bp [aes][ybcF] 46 genes
RA 562,573 G→A V80I (GTT→ATT)  sfmH → putative fimbrial adhesin protein SfmH
RA 647,062 G→A R150C (CGT→TGT)  citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 662,387 C→T V86I (GTT→ATT)  ybeD ← DUF493 domain‑containing protein YbeD
RA 671,264 A→G G114G (GGT→GGC holA ← DNA polymerase III subunit delta
RA 694,721 C→T G79S (GGC→AGC)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 709,364 A→G D344G (GAT→GGT)  chiP → chitooligosaccharide outer membrane channel
RA 723,528 (T)8→7 coding (887/2685 nt) kdpD ← sensor histidine kinase KdpD
RA 981,850 (G)6→5 coding (804/1848 nt) ldtD → L,D‑transpeptidase LdtD
MC JC 1,042,956 Δ72,555 bp [etk]mdtG 71 genes
RA 1,350,723 T→C N106S (AAC→AGC)  fabI ← enoyl‑[acyl‑carrier‑protein] reductase
MC JC 1,351,171 Δ239,229 bp ycjDydeT 230 genes
RA 1,590,430 T→C intergenic (‑429/+424) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,782,065 (G)5→4 coding (671/1290 nt) ydiS → putative electron transfer flavoprotein‑quinone oxidoreductase YdiS
RA 1,789,654 T→C D14D (GAT→GAC ydiE → PF10636 family protein YdiE
RA 1,905,761 G→A G25D (GGT→GAT)  mntP → Mn(2(+)) exporter
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
MC JC 2,085,042 Δ109,115 bp [tsuA][mrp] 106 genes
MC JC 2,286,372 Δ22,969 bp yejO[alkB] 24 genes
RA 2,311,650 A→G F367S (TTC→TCC)  ompC ← outer membrane porin C
MC JC 2,312,100 24707 bp→A [ompC][gyrA] 16 genes
MC JC 2,348,550 Δ41,411 bp [yfaE][yfbP] 40 genes
RA 2,389,974 T→G intergenic (+10/+74) yfbP → / ← nuoN uncharacterized protein YfbP/NADH:quinone oxidoreductase subunit N
RA 2,390,295 T→G Q404P (CAG→CCG)  nuoN ← NADH:quinone oxidoreductase subunit N
RA 2,424,640 T→C Q198Q (CAA→CAG hisM ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM
RA 2,531,483 T→A S7S (TCT→TCA cysZ → sulfate:H(+) symporter
RA 2,756,045 T→C intergenic (+90/‑114) ssrA → / → intA tmRNA/integrase IntA
RA 2,756,047 T→C intergenic (+92/‑112) ssrA → / → intA tmRNA/integrase IntA
MC JC 2,756,415 Δ57,799 bp [intA]emrB 53 genes
RA 2,823,802 A→G intergenic (‑33/+47) recA ← / ← pncC DNA recombination/repair protein RecA/NMN aminohydrolase
MC JC 2,823,891 Δ42,638 bp [pncC]ygbN 41 genes
RA 2,880,912 A→G I46T (ATC→ACC)  casD ← type I‑E CRISPR system Cascade subunit CasD
RA 2,925,035 C→T T82T (ACG→ACA syd ← SecY‑interacting protein
RA 2,940,568 T→C T226A (ACC→GCC)  rlmM ← 23S rRNA 2'‑O‑ribose C2498 methyltransferase
RA 3,152,939 A→G E235G (GAA→GGA)  metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase/alanine racemase
RA 3,194,362 (G)6→7 coding (1499/1662 nt) yqiK → flotillin family inner membrane protein YqiK
RA 3,214,325 T→C F427L (TTC→CTC)  rpoD → RNA polymerase sigma factor RpoD
RA 3,216,473 A→G Y7H (TAC→CAC)  nfeF ← NADPH‑dependent ferric‑chelate reductase
MC JC 3,216,779 53253 bp→GTC [nfeR]yhaC 50 genes
RA 3,297,193 G→A L316L (CTG→TTG)  yraQ ← permease family protein YraQ
RA 3,304,813 (C)7→6 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,321,967 A→G intergenic (‑354/+105) yhbX ← / ← leuU putative hydrolase YhbX/tRNA‑Leu
RA 3,436,697 G→A E60E (GAG→GAA trkA → NAD‑binding component of Trk potassium transporters
RA 3,449,779 G→A S35S (AGC→AGT rpsC ← 30S ribosomal subunit protein S3
RA 3,468,768 C→T W362* (TGG→TGA chiA ← endochitinase
RA 3,474,425 T→C K43R (AAA→AGA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,523,800 C→G Y310* (TAC→TAG mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,718,336 A→C *237E (TAA→GAA)  yiaF ← DUF3053 domain‑containing protein YiaF
RA 3,725,393 G→A intergenic (‑75/+20) glyQ ← / ← ysaB glycine‑‑tRNA ligase subunit alpha/putative lipoprotein YsaB
RA 3,725,582 C→A C44F (TGC→TTC)  ysaB ← putative lipoprotein YsaB
MC JC 3,725,590 46159 bp→ATCG [ysaB]yibI 41 genes
RA 3,834,428 (G)6→5 coding (110/2319 nt) yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,854,281 A→G L120L (TTA→TTG emrD → multidrug efflux pump EmrD
RA 3,922,994 T→C intergenic (‑554/+63) atpI ← / ← rsmG ATP synthase accessory factor AtpI/16S rRNA m(7)G527 methyltransferase
RA 3,939,662 C→T M317I (ATG→ATA hsrA ← putative transporter HsrA
RA 4,026,473 Δ1 bp coding (1486/1494 nt) ubiD → 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
RA 4,033,572 T→C I143T (ATC→ACC)  trkH → K(+) transporter TrkH
RA 4,106,199 A→G E127G (GAG→GGG)  cpxP → periplasmic protein CpxP
RA 4,123,930 A→T V176E (GTA→GAA)  cytR ← DNA‑binding transcriptional repressor CytR
RA 4,174,041 A→T E320D (GAA→GAT birA → DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
RA 4,204,890 C→T G348S (GGT→AGT)  purD ← phosphoribosylamine‑‑glycine ligase
RA 4,241,235 A→G G340G (GGT→GGC xylE ← D‑xylose:H(+) symporter
RA 4,278,657 T→C V60A (GTT→GCT)  ghxP → guanine/hypoxanthine transporter GhxP
RA 4,278,682 C→T A68A (GCC→GCT ghxP → guanine/hypoxanthine transporter GhxP
RA 4,279,023 T→C F182S (TTC→TCC)  ghxP → guanine/hypoxanthine transporter GhxP
RA 4,279,034 Δ2 bp coding (556‑557/1350 nt) ghxP → guanine/hypoxanthine transporter GhxP
MC JC 4,279,050 Δ82,373 bp [ghxP][cadC] 72 genes
RA 4,362,045 T→C intergenic (‑111/+506) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe
RA 4,444,533 A→G D141G (GAC→GGC)  tamB → translocation and assembly module subunit TamB
MC JC 4,488,473 Δ111,735 bp yjgRyjjA 100 genes
RA 4,600,215 +C intergenic (‑26/+23) yjjA ← / ← dnaC DUF2501 domain‑containing protein YjjA/DNA replication protein DnaC
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,618,638 +C coding (410/1323 nt) deoA → thymidine phosphorylase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 263050 388707 125658 21 [19] [20] 21 ykfI–tauD ykfI,yafW,ykfH,ykfG,yafX,ykfF,ykfB,yafY,yafZ,ykfA,perR,insN,insI1,insN,eyeA,ykfC,insH1,mmuP,mmuM,afuC,insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,insF1,rclC,rclB,rclA,rclR,ykgE,ykgF,ykgG,ykgH,betA,betB,betI,betT,yahV,pdeL,yahB,yahC,yahD,yahE,yahF,yahG,yahH,yahI,yahJ,yahK,yahL,yahM,yahN,yahO,prpR,prpB,prpC,prpD,prpE,codB,codA,cynR,cynT,cynS,cynX,lacA,lacY,lacZ,lacI,mhpR,mhpA,mhpB,mhpC,mhpD,mhpF,mhpE,mhpT,yaiL,frmB,frmA,frmR,yaiO,yaiX,insC1,insD1,yaiX,yaiP,ykiC,yaiS,tauA,tauB,tauC,tauD
* * ÷ NC_000913 787553 922303 134751 21 [20] [18] 21 [gpmA]–macB [gpmA],galM,galK,galT,galE,modF,modE,acrZ,modA,modB,modC,ybhA,pgl,ybhD,ybhH,ybhI,ybhJ,ybhC,ybhB,bioA,bioB,bioF,bioC,bioD,uvrB,ybhK,moaA,moaB,moaC,moaD,moaE,ybhL,ybhM,ybhN,clsB,ybhP,ybhQ,ybhR,ybhS,ybhF,ybhG,cecR,rhlE,ybiA,dinG,ybiB,hcxB,zbiJ,ybiE,ybiJ,ybiI,ybiX,fiu,mcbA,rlmF,ybiO,glnQ,glnP,glnH,dps,rhtA,yliM,ompX,opgE,rybA,mntS,mntR,ybiR,ldtB,ybiT,ybiU,ybiV,ybiW,ybiY,fsaA,moeB,moeA,iaaA,gsiA,gsiB,gsiC,gsiD,pdeI,dgcI,rimO,bssR,yliI,gstB,dacC,deoR,ybjG,mdfA,ybjH,ybjI,ybjJ,rcdA,rybB,ybjL,ybjM,grxA,ybjC,nfsA,rimK,ybjN,potF,potG,potH,potI,ybjO,rlmC,artJ,artM,artQ,artI,artP,ybjP,ybjQ,amiD,ybjS,ybjT,ltaE,poxB,hcr,hcp,lysO,aqpZ,ybjD,ybjX,macA,macB
* * ÷ NC_000913 1591211 1652830 61620 21 [20] [20] 21 [hipA]–[ynfP] [hipA],hipB,lsrK,lsrR,lsrA,lsrC,lsrD,lsrB,lsrF,lsrG,tam,yneE,uxaB,dgcF,yneG,glsB,sad,yneJ,yneK,ydeA,marC,marR,marA,marB,eamA,ydeE,mgtS,mgtT,mgrR,dgcZ,ydeI,ydeJ,dcp,ydfG,rspR,ydfZ,ydfI,ydfJ,ynfT,ydfK,pinQ,tfaQ,stfQ,nohA,ynfO,ydfO,gnsB,ynfN,ynfR,ynfQ,cspI,rzpQ,rzoQ,rrrQ,ydfR,essQ,ynfU,cspB,cspF,ynfS,ydfT,ydfU,rem,hokD,relE,relB,ydfV,flxA,ydfW,ydfX,dicC,dicA,ydfA,ydfB,ydfC,dicF,dicB,ydfD,[ynfP]
* * ÷ NC_000913 3423834–3424234 3424451–3424238 5–618 21 [20] [20] 21 [rrlD] [rrlD]
* * ÷ NC_000913 3619001 3718329 99329 21 [20] [20] 24 rhsB–ghrB rhsB,yhhH,yhhI,yhhJ,rbbA,yhiI,yhiY,yhiJ,yhiM,yhiN,pitA,uspB,uspA,yriA,yriB,dtpB,rsmJ,prlC,rlmJ,gor,dinQ,agrA,agrB,arsR,arsB,arsC,yhiS,insH11,yhiS,slp,dctR,yhiD,hdeB,hdeA,hdeD,arrS,gadE,gadF,mdtE,mdtF,gadW,gadY,gadX,gadA,ccp,treF,yhjB,rcdB,yhjD,yhjE,yhjG,pdeH,kdgK,yhjJ,dctA,pdeK,bcsC,bcsZ,bcsB,bcsA,yhjR,bcsE,bcsF,bcsG,ldrD,rdlD,yhjV,dppF,dppD,dppC,dppB,dppA,proK,eptB,yhjX,yhjY,tag,yiaC,bisC,yiaD,ghrB

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =44 (0.460)48 (0.540) 47/448 0.4 57.1% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 31 (0.350)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780543 (0.450)51 (0.570) 44/448 0.5 58.9% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 31 (0.350)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)48 (0.510) 44/472 0.6 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein