Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,444,533 A→G D141G (GAC→GGC)  tamB → translocation and assembly module subunit TamB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,444,5330AG99.1% 428.2 / NA 114D141G (GAC→GGC) tamBtranslocation and assembly module subunit TamB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (58/55);  minor base T (1/0);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 114 total aligned reads displayed.

CCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCC  >  NC_000913/4444287‑4444776
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
cctgatcggACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAACGCGGGCAATCTGTTTCTGGCTGTCGGGTTTGAGTGCTTGTGGAACAG‑AATGCTTTGTATAAATGATCTGGCGCTGAAAGACATTCAGGTAAACATCAACAGAAAAAAAATGCCGCCTTCTGAA‑CAGGTTGAAGAAAAGGAAGAAAGCGGT‑CCGCTGAACCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAAc                                                                                                                                                                                                                                                 <  2:660082/243‑1 (MQ=255)
  ttGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGt                                                                                                                                                                                                                                               >  1:137468/1‑251 (MQ=255)
   tGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGt                                                                                                                                                                                                                                               <  1:233946/250‑1 (MQ=255)
    ntCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGG‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTc                                                                                                                                                                                                                                              >  2:306006/2‑250 (MQ=255)
    gTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAAAGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTc                                                                                                                                                                                                                                              >  1:311122/1‑250 (MQ=255)
     tCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGGGAGCCTGTGGAACCGCAGTG‑TTTGTTTTAATGAACTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGCTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGAACTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCCCTGGGCAACggca                                                                                                                                                                                                                                             >  2:506857/1‑247 (MQ=255)
      cTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAAc                                                                                                                                                                                                                                           >  1:648714/1‑251 (MQ=255)
           gttcgttATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGGGGCACTGGGCAACGTCAACCTTa                                                                                                                                                                                                                                       >  2:643004/1‑250 (MQ=255)
               gttATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGGTTTAAAGGCTGTGGAACAGTAGTG‑TTTGTATTAATGATCTGGCGCCGAAAGACATTCAGGCCAACATCGACAGAAAAAAAATGCCTTCTTCTGAA‑CAGGCTGAAGAAGAGGAAGATAGCGGT‑CCGCCGGATCTCTCCACCCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGAtt                                                                                                                                                                                                                                  >  1:635258/1‑251 (MQ=255)
               gttATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGGGGCACTGGGCAACGTCCACATTAAGAtt                                                                                                                                                                                                                                  >  1:131716/1‑251 (MQ=255)
                  aTGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATtgat                                                                                                                                                                                                                               >  1:137733/1‑251 (MQ=255)
                       cagcCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGGTGTAGGGCTTGAGGGCCTGTGGAACCGAAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑AAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTTCCCGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGacac                                                                                                                                                                                                                          >  2:618246/1‑251 (MQ=255)
                          ccAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCGGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGAcaccac                                                                                                                                                                                                                       <  2:137468/251‑1 (MQ=255)
                           cAGGCGTGGCGGAAAAAGCGGGCAATCTGCATCGGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACg                                                                                                                                                                                                                      <  2:137733/251‑1 (MQ=255)
                               ggTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAAGGATCTGGCGCTGAAAGACATTCAGGTTAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTAt                                                                                                                                                                                                                  <  1:660106/250‑1 (MQ=255)
                                 gggCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCg                                                                                                                                                                                                                <  1:217894/250‑1 (MQ=255)
                                     ggTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTgt                                                                                                                                                                                                            >  2:604796/1‑250 (MQ=255)
                                           aGCGGGCAATCTACATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACt                                                                                                                                                                                                      >  1:481682/1‑251 (MQ=255)
                                              gggCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCa                                                                                                                                                                                                   <  1:376675/251‑1 (MQ=255)
                                                gCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCAcc                                                                                                                                                                                                 <  2:459969/251‑1 (MQ=255)
                                                  attCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAAGGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTc                                                                                                                                                                                               <  2:48792/249‑1 (MQ=255)
                                                   aTCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAAc                                                                                                                                                                                                                                           >  1:13462/1‑206 (MQ=255)
                                                   aTCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTGGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAAc                                                                                                                                                                                                                                           <  2:13462/206‑1 (MQ=255)
                                                     cTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCgg                                                                                                                                                                                            >  1:130109/1‑251 (MQ=255)
                                                     cTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCgg                                                                                                                                                                                            >  1:394932/1‑251 (MQ=255)
                                                       gCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGcc                                                                                                                                                                                          >  1:100432/1‑251 (MQ=255)
                                                                tGTCGGGCTTGAGTGCCTGTGGAACGGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTg                                                                                                                                                                                        >  1:322800/1‑244 (MQ=255)
                                                                tGTCGGGCTTGAGTGCCTGTGGAACGGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTGGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCtn                                                                                                                                                                                        <  2:322800/244‑2 (MQ=255)
                                                                        ttGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGaa                                                                                                                                                                          >  2:355139/1‑250 (MQ=255)
                                                                           aGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAacc                                                                                                                                                                      >  1:585453/1‑251 (MQ=255)
                                                                               ccTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTcc                                                                                                                                                                                              >  2:491444/1‑223 (MQ=255)
                                                                               ccTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTcc                                                                                                                                                                                              <  1:491444/223‑1 (MQ=255)
                                                                                  gtgGAACAGCGGTG‑TTTGTATTAATGATCTGGCGCTAAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGTATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAaccctgacc                                                                                                                                                                <  1:96594/250‑1 (MQ=255)
                                                                                  gtgGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAaccctgaccc                                                                                                                                                               >  1:3576/1‑251 (MQ=255)
                                                                                    ggAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCa                                                                                                                                                                                                   <  1:292691/213‑1 (MQ=255)
                                                                                        cagcagTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAAc                                                                                                                                                         <  1:306006/251‑1 (MQ=255)
                                                                                          gcagTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCg                                                                                                                                                       >  1:585334/1‑251 (MQ=255)
                                                                                          gcagTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCg                                                                                                                                                       >  2:601549/1‑251 (MQ=255)
                                                                                                  ttttATTAAGGATCGGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCg                                                                                                                                                 <  2:131716/247‑1 (MQ=255)
                                                                                                         aaTGGTCTGGCACGGAAAGACATTAAGGTAAACACGGACAGTAAAAAAACGCCTCCTGCGGAC‑CAGTTTGAAGAAGAGAAAGATAGCGGC‑CCGCTGGATCTCCCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAg                                                                                                                                          <  2:47807/250‑1 (MQ=255)
                                                                                                            gaccTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGAc                                                                                                                                                                                                       <  1:17954/183‑1 (MQ=255)
                                                                                                            gATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGAc                                                                                                                                                                                                       >  2:17954/1‑186 (MQ=255)
                                                                                                               cTGGCGCTAAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATtgatga                                                                                                                                                                                                                             <  2:448081/161‑1 (MQ=255)
                                                                                                                      tGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                                            >  1:695894/1‑251 (MQ=255)
                                                                                                                       gAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGctc                                                                                                                           >  1:186066/1‑251 (MQ=255)
                                                                                                                       gAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGctc                                                                                                                           >  1:245612/1‑251 (MQ=255)
                                                                                                                       gAAAGACAGTCAGTACAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGC‑CCGCTGGACCTCCCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGct                                                                                                                            <  2:432805/250‑1 (MQ=255)
                                                                                                                       aaaaGACATTCAGGTAAACATGGAAAGAAAAAAAATGCCTCCT‑GTGAAACAGGTTGAAGAAGAGGAAGATAG‑GGTGCCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGc                                                                                                                             <  1:27849/248‑1 (MQ=255)
                                                                                                                        aaaGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTg                                                                                                                                                                                   <  1:508067/194‑1 (MQ=255)
                                                                                                                        aaaGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTg                                                                                                                                                                                   >  2:508067/1‑194 (MQ=255)
                                                                                                                        aaaGACATTCAGGCCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGAAAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGctct                                                                                                                          <  2:100432/251‑1 (MQ=255)
                                                                                                                             cATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCg                                                                                                                      >  2:474384/1‑250 (MQ=255)
                                                                                                                                    gTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGc                                                                                                              <  1:337416/251‑1 (MQ=255)
                                                                                                                                    gTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGc                                                                                                              >  1:508669/1‑251 (MQ=255)
                                                                                                                                      cAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCACATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAAACCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCgg                                                                                                            >  2:501626/1‑251 (MQ=255)
                                                                                                                                                    aaaaaaTGCCTCCTTCTGAA‑CAGGTTGCAGAAGAGGAAGATAGCGGT‑CCGCTGGCTCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGCGAAAACCCTGCCCCTGAACCCGACGTCGCTGCAAGGCCTGCTGATTTCTCTGCCGAAAGTGGCGGTAGAGGAGCAggg                                                                                               >  2:329444/1‑249 (MQ=255)
                                                                                                                                                    aaaaaaTGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGga                                                                                               >  1:548218/1‑250 (MQ=255)
                                                                                                                                                    aaaaaaTGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCGGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGgaa                                                                                              <  2:181088/251‑1 (MQ=255)
                                                                                                                                                     aaaaaTGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGCAGTGGCGCAGga                                                                                               >  1:168943/1‑249 (MQ=255)
                                                                                                                                                         aTGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGt                                                                                         <  2:55335/251‑1 (MQ=255)
                                                                                                                                                           gCCTCCTTCTGAA‑CAGTTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTgn                                                                                       <  2:311122/251‑2 (MQ=255)
                                                                                                                                                              ccctTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCGCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCg                                                                                    <  2:358299/250‑1 (MQ=255)
                                                                                                                                                                     gAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGaa                                                                             <  1:474384/251‑1 (MQ=255)
                                                                                                                                                                      aa‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGGGCAGGAAGAAGGGGTCGAACCGaa                                                                             >  2:408604/1‑250 (MQ=255)
                                                                                                                                                                          aGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAAtt                                                                         >  1:143285/1‑251 (MQ=255)
                                                                                                                                                                           ggTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGCCACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCACGAAAAAGCGGACGAACCCAAAATTg                                                                        >  1:659695/1‑251 (MQ=255)
                                                                                                                                                                            gTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGGCGAACCGGAAATTg                                                                        >  1:262568/1‑250 (MQ=255)
                                                                                                                                                                              tGAAGAAAAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGaa                                                                      <  2:548218/250‑1 (MQ=255)
                                                                                                                                                                               gaagaaGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATtgat                                                                                                                                                                                                                               >  1:288297/1‑96 (MQ=255)
                                                                                                                                                                               gaagaaGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATtgat                                                                                                                                                                                                                               <  2:288297/96‑1 (MQ=255)
                                                                                                                                                                                aagaagAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGCAGGAAGAAGTGGGCGAACCGAAAATTGaaaa                                                                    >  2:63419/1‑250 (MQ=255)
                                                                                                                                                                                aagaagAGGAAGATAGCGGC‑CCGCTGGATCTCACCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGaaaan                                                                   <  2:327771/251‑2 (MQ=255)
                                                                                                                                                                                 agaagaGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATc                                                                  >  1:585870/1‑251 (MQ=255)
                                                                                                                                                                                    agagGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGc                                                               <  1:408604/251‑1 (MQ=255)
                                                                                                                                                                                    agagGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGc                                                               <  1:355139/251‑1 (MQ=255)
                                                                                                                                                                                     gagGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCa                                                              <  2:186066/251‑1 (MQ=255)
                                                                                                                                                                                     gagGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCa                                                              <  2:648714/251‑1 (MQ=255)
                                                                                                                                                                                       ggAAGATAGCGTT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCTACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGc                                                            <  1:645678/251‑1 (MQ=255)
                                                                                                                                                                                        gAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGGGGCGGAGGGAGGAGGGGGCGAGCCGAAAATTGTAAATCCGCAGcc                                                           >  2:24935/1‑251 (MQ=255)
                                                                                                                                                                                          aGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGGCGAACCGAAAATTGAAAATCCGCAGCCgg                                                         >  1:10077/1‑251 (MQ=255)
                                                                                                                                                                                            aTAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGAt                                                       <  2:394932/251‑1 (MQ=255)
                                                                                                                                                                                            aTAGCGGT‑CCGCGGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGAt                                                       <  2:53393/251‑1 (MQ=255)
                                                                                                                                                                                              aGCGGT‑CCGCTGGATCTCTCCACGCCGGAGCCCATCACCATGACACGGGGGGAACTGGGCAACGGAAACAGTAAGATTGAGGACACCAAGGCATCGGAGATGGACTACACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGGCGCGGAAAGGCCTGCTGATGGCTCTGACGAAAGTGGCGGAAAAGGTGAAGGAAGCAGTGATAGAACCGAAAATTGAAAATCCGCAGCCGGATg                                                      >  2:130987/1‑250 (MQ=255)
                                                                                                                                                                                                  gT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGAc                                                                                                                                                     <  1:607503/151‑1 (MQ=255)
                                                                                                                                                                                                  gT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGAc                                                                                                                                                     >  2:607503/1‑151 (MQ=255)
                                                                                                                                                                                                  gT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGaaa                                                   >  1:31513/1‑249 (MQ=255)
                                                                                                                                                                                                     ccGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGcc                                               <  2:31513/251‑1 (MQ=255)
                                                                                                                                                                                                     ccGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGc                                                <  1:643004/250‑1 (MQ=255)
                                                                                                                                                                                                          ggacccctcCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCt                                            <  2:245612/243‑1 (MQ=255)
                                                                                                                                                                                                          ggATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCCGGAAGAAGGGGTCGAAACGAAAATTGAAAATCCGCAGCCGGGTGAAAAGccccacg                                          >  2:643102/1‑246 (MQ=255)
                                                                                                                                                                                                            aTCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGc                                        >  1:627801/1‑251 (MQ=255)
                                                                                                                                                                                                             gctctcCACTCCGTATCCCATCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCg                                       <  2:635258/250‑1 (MQ=255)
                                                                                                                                                                                                                           aTCCCCTCACCCTGACACGGGTGGCACTGTTCCCCGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTTACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGAATCCTCTGCCGACAGTGGCGGAAGTGGCGCAGGAAGAAGGGGTCGAACCGAAAATTCAAAAACCCCAGCCGGCTGCAAAGCCCCTCGGCGAAACGCTGAAAGac                         >  2:25065/1‑250 (MQ=255)
                                                                                                                                                                                                                                aTCACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATctctn                    <  2:318875/251‑2 (MQ=255)
                                                                                                                                                                                                                                  cACCCTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCttt                   <  1:601549/250‑1 (MQ=255)
                                                                                                                                                                                                                                   ccccTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTtc                 <  1:604796/250‑1 (MQ=255)
                                                                                                                                                                                                                                     ccTGACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTtctc               <  1:577490/251‑1 (MQ=255)
                                                                                                                                                                                                                                        gACACGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCg              <  1:467894/249‑1 (MQ=255)
                                                                                                                                                                                                                                          cacGGGTGGCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGCAGTGGCGCAGGAAGAAGTGGTCGAACCGGAAATTGAAAATCCCCAGCCGGATGAAAAGCCGCTCGGGGAAACGCTGAAAGATCTCTTTTCTCGCCCg          >  2:660523/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  gCACTGGGCAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTAGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGcc  >  1:227805/1‑251 (MQ=255)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   
CCTTGTCTGACGTTCGTTATGAGCAGCCAGGCGTGGCGGTAAAAGCGGGCAATCTGCATCTGGCTGTCGGGCTTGAGTGCCTGTGGAACAGCAGTG‑TTTGTATTAATGATCTGGCGCTGAAAGACATTCAGGTCAACATCGACAGTAAAAAAATGCCTCCTTCTGAA‑CAGGTTGAAGAAGAGGAAGATAGCGGT‑CCGCTGGATCTCTCCACGCCGTATCCCATCACCCTGACACGGGTGGCACTGGACAACGTCAACATTAAGATTGATGACACCACGGTATCGGTGATGGACTTCACCTCCGGCCTGAACTGGCAGGAGAAAACCCTGACCCTGAAACCGACGTCGCTGAAAGGCCTGCTGATTGCTCTGCCGAAAGTGGCGGAAGTGGCGCAGGAAGAAGTGGTCGAACCGAAAATTGAAAATCCGCAGCCGGATGAAAAGCCGCTCGGCGAAACGCTGAAAGATCTCTTTTCTCGCCCGGTATTGCC  >  NC_000913/4444287‑4444776

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑