Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,922,994	T→C	intergenic (‑554/+63)	atpI ← / ← rsmG	ATP synthase accessory factor AtpI/16S rRNA m(7)G527 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,922,994	0	T	C	100.0%	226.1 / NA	57	intergenic (‑554/+63)	atpI/rsmG	ATP synthase accessory factor AtpI/16S rRNA m(7)G527 methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/31);  total (26/31)

TTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGCACCAGCTCAC  >  NC_000913/3922752‑3923239                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      ttGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGaaaa                                                                                                                                                                                                                                                 <  1:348821/249‑1 (MQ=255)   gCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAACCCt                                                                                                                                                                                                                                              <  2:549158/250‑1 (MQ=255)   gCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCt                                                                                                                                                                                                                                              <  1:358004/250‑1 (MQ=255)    cAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTgg                                                                                                                                                                                                                                            <  1:14797/251‑1 (MQ=255)           aTGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGgttgttg                                                                                                                                                                                                                                      <  2:522252/250‑1 (MQ=255)           aTGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGgttgttg                                                                                                                                                                                                                                      <  2:426602/250‑1 (MQ=255)           aTGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATAGCAGCAGAAAACCTGgttgttg                                                                                                                                                                                                                                      <  2:527898/250‑1 (MQ=255)             gATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGgttgttgtt                                                                                                                                                                                                                                    <  1:621840/250‑1 (MQ=255)                    cAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCt                                                                                                                                                                                                                            <  2:26347/251‑1 (MQ=255)                    cAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCt                                                                                                                                                                                                                            <  2:647554/251‑1 (MQ=255)                      gTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTa                                                                                                                                                                                                                           >  1:451673/1‑250 (MQ=255)                              aCAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCa                                                                                                                                                                                                                   >  2:101640/1‑250 (MQ=255)                                       gTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATg                                                                                                                                                                                                         >  1:50978/1‑251 (MQ=255)                                         tttAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGAt                                                                                                                                                                                                       >  2:661848/1‑251 (MQ=255)                                          ttAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGAtt                                                                                                                                                                                                      >  1:353354/1‑251 (MQ=255)                                                     cATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATaa                                                                                                                                                                                            >  2:507185/1‑250 (MQ=255)                                                           cacaACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAaaattaa                                                                                                                                                                                     <  2:605393/251‑1 (MQ=255)                                                              aacaaAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAaaattaaatt                                                                                                                                                                                  <  1:586274/251‑1 (MQ=255)                                                                    aaCCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTAttt                                                                                                                                                                            <  1:101640/251‑1 (MQ=255)                                                                       caccCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCt                                                                                                                                                                         >  2:517655/1‑251 (MQ=255)                                                                         cccATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCttt                                                                                                                                                                       <  1:374411/251‑1 (MQ=255)                                                                            aTTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTCATTTGCTTTAAt                                                                                                                                                                    <  1:483845/251‑1 (MQ=255)                                                                                aCATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATcacc                                                                                                                                                                >  1:597120/1‑251 (MQ=255)                                                                                        aataatGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATg                                                                                                                                                        <  1:360006/251‑1 (MQ=255)                                                                                              gTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAGCCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTc                                                                                                                                                  >  2:333705/1‑251 (MQ=255)                                                                                                tttAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGc                                                                                                                                                >  1:246480/1‑251 (MQ=255)                                                                                                    aCAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATc                                                                                                                                            <  2:439875/251‑1 (MQ=255)                                                                                                                 gTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAAcc                                                                                                                               <  2:184490/251‑1 (MQ=255)                                                                                                                                 ttgaGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCTCCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACt                                                                                                                <  1:467252/247‑1 (MQ=255)                                                                                                                                  ttAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAACAAAGCCACGTTCTCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTg                                                                                                               >  2:637506/1‑250 (MQ=255)                                                                                                                                   tAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGa                                                                                                              <  2:114836/250‑1 (MQ=255)                                                                                                                                     gCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTc                                                                                                           <  2:246480/251‑1 (MQ=255)                                                                                                                                     gCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTc                                                                                                           <  2:392889/251‑1 (MQ=255)                                                                                                                                                     aCTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGa                                                                                                                                                                                                                                                    >  1:552804/1‑98 (MQ=255)                                                                                                                                                     aCTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGa                                                                                                                                                                                                                                                    <  2:552804/98‑1 (MQ=255)                                                                                                                                                               tATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGcaacaa                                                                                   >  2:258526/1‑249 (MQ=255)                                                                                                                                                                       gCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTc                                                                          >  2:693459/1‑250 (MQ=255)                                                                                                                                                                            ccAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTc                                                                    <  1:507185/251‑1 (MQ=255)                                                                                                                                                                             cAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTcc                                                                   <  2:501014/251‑1 (MQ=255)                                                                                                                                                                              aaaaaaaGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCg                                                                  >  1:77296/1‑251 (MQ=255)                                                                                                                                                                              aaaaaaaGCCACGTTATCTGGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCg                                                                  <  2:353354/251‑1 (MQ=255)                                                                                                                                                                                 acaaGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGc                                                                >  2:367129/3‑250 (MQ=255)                                                                                                                                                                                   aaGCCACGTTATCTTGTTGATGCAAAAGAATGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCAtt                                                             >  1:475374/1‑251 (MQ=255)                                                                                                                                                                                       cACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAGCCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGcc                                                         <  1:333705/251‑1 (MQ=255)                                                                                                                                                                                          gTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCtt                                                      <  2:702577/251‑1 (MQ=255)                                                                                                                                                                                                 tgttgATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCa                                                   <  2:181476/247‑1 (MQ=255)                                                                                                                                                                                                  gttgATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGcgcg                                              >  2:140878/1‑251 (MQ=255)                                                                                                                                                                                                  gttgATGCAAAAGAGCGAACGCGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGcgcg                                              >  2:396791/1‑251 (MQ=255)                                                                                                                                                                                                     gATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAg                                           <  2:457885/251‑1 (MQ=255)                                                                                                                                                                                                      aTGCAAAAGAGTAAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGt                                             >  1:496016/1‑248 (MQ=255)                                                                                                                                                                                                         naaaaGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAAc                                       >  2:325316/2‑251 (MQ=255)                                                                                                                                                                                                               agTGAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCtt                                 >  1:191280/1‑251 (MQ=255)                                                                                                                                                                                                                  gAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGa                                                                                                                            <  1:601735/157‑1 (MQ=255)                                                                                                                                                                                                                  gAACGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGa                                                                                                                            >  2:601735/1‑157 (MQ=255)                                                                                                                                                                                                                   aaCGTGGCGTTAAATGTAACCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTc                             >  2:444491/1‑251 (MQ=255)                                                                                                                                                                                                                                    aaCCAGTTATATCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGc            >  1:312149/1‑251 (MQ=255)                                                                                                                                                                                                                                              atCAGCAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGCACCAGCTCAc  >  2:368352/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      TTGCAAGTTTATGATATTTCAGTCTAAAAACAGATACTGTTTTAATAAATGACATTTACACAACAAAAACCACCCATTGACATTTTTAATAATGTTTTAACAGCCAATGATGGTTCTTAGCGCCGATTTTTAGCAGACTGATATTTTCACTAATGACTTATTTTCTGCTTACCAAAAAAAGCCACGTTATCTTGTTGATGCAAAAGAGTGAACGTGGCGTTAAATGTAACCAGTTATATCAGTAGAAAACCTGGTTGTTGTTAACAGTCTAACCGGTCAATTTTTTATGATTTTTTTGATAAAAATTAAATTTTATTTGCTTTAATCACCACCAGATGACGTTCGCCATCCAGGGCTGGAACCTGAAGTTTAACCACTGATTCGACCTGATATTCTTCGGGCAACAAAGCGATTTCATCTTCCGGCATTTGCCCTTTCAGCGCGTAGAAACGGCCTTGCTCACCAGGAAGATGGTGGCACCAGCTCAC  >  NC_000913/3922752‑3923239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑