Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,321,967	A→G	intergenic (‑354/+105)	yhbX ← / ← leuU	putative hydrolase YhbX/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,321,967	0	A	G	100.0%	299.0 / NA	78	intergenic (‑354/+105)	yhbX/leuU	putative hydrolase YhbX/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/43);  total (35/43)

ATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGG  >  NC_000913/3321734‑3322205                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               atgatgCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAAc                                                                                                                                                                                                                               >  2:183369/1‑251 (MQ=255)   gatgCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTg                                                                                                                                                                                                                             >  1:361035/1‑251 (MQ=255)    atgCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTgg                                                                                                                                                                                                                            <  2:268027/251‑1 (MQ=255)      gCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGAc                                                                                                                                                                                                                          <  1:477125/251‑1 (MQ=255)           cATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGagat                                                                                                                                                                                                                     <  1:547836/251‑1 (MQ=255)           cATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGagat                                                                                                                                                                                                                     <  1:401691/251‑1 (MQ=255)               gCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGagatacag                                                                                                                                                                                                                 <  1:113126/251‑1 (MQ=255)                 gTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGagatacagat                                                                                                                                                                                                               <  2:361035/251‑1 (MQ=255)                  ttCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGagatacagat                                                                                                                                                                                                               >  1:593322/1‑250 (MQ=255)                    ctcAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATAcc                                                                                                                                                                                                            >  2:412720/1‑251 (MQ=255)                         tAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGAc                                                                                                                                                                                                        >  1:455289/1‑250 (MQ=255)                          aaTTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACa                                                                                                                                                                                                       >  1:465575/1‑250 (MQ=255)                            ttAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAAcc                                                                                                                                                                                                    >  1:9101/1‑251 (MQ=255)                                           aaTGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATCCCTCAGACCAgg                                                                                                                                                                                     <  1:488324/251‑1 (MQ=255)                                                      aaaCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGa                                                                                                                                                                           >  1:245031/1‑250 (MQ=255)                                                       aaCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGaaa                                                                                                                                                                         <  1:183369/251‑1 (MQ=255)                                                        aCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGa                                                                                                                                                                                          <  2:210466/233‑1 (MQ=255)                                                        aCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGa                                                                                                                                                                                          >  1:210466/1‑233 (MQ=255)                                                        aCATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGaaaa                                                                                                                                                                        <  2:9101/251‑1 (MQ=255)                                                         cATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGaaaaa                                                                                                                                                                       <  1:357032/251‑1 (MQ=255)                                                               gCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGc                                                                                                                                                                 >  2:468666/1‑251 (MQ=255)                                                                cAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCt                                                                                                                                                                <  1:161454/251‑1 (MQ=255)                                                                 aGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCtt                                                                                                                                                               <  1:310483/251‑1 (MQ=255)                                                                 aGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCtt                                                                                                                                                               >  1:595466/1‑251 (MQ=255)                                                                    aCGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                            >  2:213854/1‑251 (MQ=255)                                                                    aCGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                            <  1:139099/251‑1 (MQ=255)                                                                         gCCATCAGTCGTTATACTGACAGTAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                                       <  2:403731/251‑1 (MQ=255)                                                                         gCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGt                                                                                                                                                       <  2:403761/251‑1 (MQ=255)                                                                            aTCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGtctc                                                                                                                                                    <  2:441950/251‑1 (MQ=255)                                                                            aTCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGtctc                                                                                                                                                    <  2:481563/251‑1 (MQ=255)                                                                              cAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCtt                                                                                                                                                  <  2:159311/251‑1 (MQ=255)                                                                                gTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCtttn                                                                                                                                                <  2:317284/251‑2 (MQ=255)                                                                                   gTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCAtt                                                                                                                                                                                                 <  2:569826/199‑1 (MQ=255)                                                                                   gTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCAtt                                                                                                                                                                                                 >  1:569826/1‑199 (MQ=255)                                                                                      ataCTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGa                                                                                                                                           <  2:112450/250‑1 (MQ=255)                                                                                              aGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTAc                                                                                                                                  >  1:14810/1‑251 (MQ=255)                                                                                                gAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCg                                                                                                                                <  1:645167/251‑1 (MQ=255)                                                                                                 aGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                            >  2:150555/1‑222 (MQ=255)                                                                                                 aGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTc                                                                                                                                                            <  1:150555/222‑1 (MQ=255)                                                                                                     ggATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGAc                                                                                                                           >  1:678166/1‑251 (MQ=255)                                                                                                      gATGGTTTATCGGGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACg                                                                                                                          >  1:497132/1‑251 (MQ=255)                                                                                                      gATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACg                                                                                                                          <  2:58860/251‑1 (MQ=255)                                                                                                            ttATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGATGGGACt                                                                                                                     <  2:14810/250‑1 (MQ=255)                                                                                                                 gagaAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAGCTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACTGGACTTGAAcc                                                                                                               <  2:593322/251‑1 (MQ=255)                                                                                                                        aaaCATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCa                                                                                                                                                           >  1:684754/1‑200 (MQ=255)                                                                                                                        aaaCATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCa                                                                                                                                                           <  2:684754/200‑1 (MQ=255)                                                                                                                         aaCATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCt                                                                                                                                                                                              <  2:1450/164‑1 (MQ=255)                                                                                                                         aaCATATCCGTATTCGTACCGGAACTGGAGTTGAAACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCt                                                                                                                                                                                              >  1:1450/1‑164 (MQ=255)                                                                                                                             tatCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCttt                                                                                                                                                 >  1:194965/1‑205 (MQ=255)                                                                                                                             tatCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCttt                                                                                                                                                 <  2:194965/205‑1 (MQ=255)                                                                                                                               tCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTg                                                                                                 >  2:689914/1‑251 (MQ=255)                                                                                                                                ccGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTgg                                                                                                >  1:292216/1‑251 (MQ=255)                                                                                                                                 cGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTggg                                                                                               >  1:589527/1‑251 (MQ=255)                                                                                                                                          aCCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTaccac                                                                                      <  2:678166/251‑1 (MQ=255)                                                                                                                                            nggAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCt                                                                                    >  2:325642/2‑251 (MQ=255)                                                                                                                                             ggAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTc                                                                                   >  2:45250/1‑251 (MQ=255)                                                                                                                                                            acaATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCt                                                                     >  2:513669/1‑250 (MQ=255)                                                                                                                                                                 aaaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCCCCTCAAGGTAGCGTGTCTACCAAt                                                               <  1:557989/251‑1 (MQ=255)                                                                                                                                                                  aaGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAAtt                                                              >  2:124924/1‑251 (MQ=255)                                                                                                                                                                      gCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTccac                                                          <  2:203892/251‑1 (MQ=255)                                                                                                                                                                            tATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCt                                                     <  2:455289/250‑1 (MQ=255)                                                                                                                                                                            tATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTc                                                    >  2:340566/1‑251 (MQ=255)                                                                                                                                                                                   gTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACgg                                             >  2:417156/1‑251 (MQ=255)                                                                                                                                                                                     aGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                                                            >  2:286978/1‑170 (MQ=255)                                                                                                                                                                                     aGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGa                                                                                                                            <  1:286978/170‑1 (MQ=255)                                                                                                                                                                                        aGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGAtact                                        <  2:497132/251‑1 (MQ=255)                                                                                                                                                                                         gggAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCgggtgacgacca                                        >  2:620343/1‑240 (MQ=255)                                                                                                                                                                                              aaaTTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACtttt                                  >  2:490325/1‑251 (MQ=255)                                                                                                                                                                                                 tttATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACttttt                                 >  2:512636/1‑249 (MQ=255)                                                                                                                                                                                                    ataACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTc                            <  1:512636/251‑1 (MQ=255)                                                                                                                                                                                                          aaGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGata                      <  1:325642/251‑1 (MQ=255)                                                                                                                                                                                                          aaGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGat                       <  1:213854/250‑1 (MQ=255)                                                                                                                                                                                                          aaGGCGTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGa                        <  2:245031/249‑1 (MQ=255)                                                                                                                                                                                                               gTACCGGCACCATCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGcn                 <  2:317453/251‑2 (MQ=255)                                                                                                                                                                                                                          aTCGTTTCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAg      <  2:292216/251‑1 (MQ=255)                                                                                                                                                                                                                                tCAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCgg  >  1:699241/1‑249 (MQ=255)                                                                                                                                                                                                                                 cAAGGTACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                                                           <  1:566002/175‑1 (MQ=255)                                                                                                                                                                                                                                 cAAGGAACCGGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGc                                                                           >  2:566002/1‑175 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                               ATGATGCGGCCATTGCGTTCTCAGTAATTAAATAACATTATCAATGCGTCTCTAAACATTACGCAGAACGTAGCCATCAGTCGTTATACTGACAGGAGCAGGATGGTTTATCGAGAAGTAAACATATCCGTATTCGTACCGGAACTGGAGTTGACACAATAAAGTGCCAATTATGTCAGTAGAAGGGAAAAATTTATAACTAAGGCGTACCGGCACCATCGTTTCAAGGTACCAGCTACGAGTAAAGCAACTGGACGAGATACAGATACCTGACAACCATTCCTCAGACCAGGACCAAAACGAAAAAAGACGCTTTTCAGCGTCTCTTTTCTGGAATTTGGTACCGAGGACGGGACTTGAACCCGTAAGCCCTATTGGGCACTACCACCTCAAGGTAGCGTGTCTACCAATTCCACCACCTCGGCACGGATACTACTTTTTAGTTCGGGATATCGCTGGTCGGCTTAGCCGG  >  NC_000913/3321734‑3322205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑