Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,590,430	T→C	intergenic (‑429/+424)	ydeT ← / ← hipA	fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,590,430	0	T	C	100.0%	304.9 / NA	80	intergenic (‑429/+424)	ydeT/hipA	fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/39);  total (41/39)

CTAAACAGAATATTATTGCGAACAAGGGAAGTGTCAGGAAAGGTCAAGAGCTACCAATGGTTAATATGGCTATAAATCATTAGCTATGGTTAATGTTTGCTTGCTGAAACTAACACCGAATTGCTCTAAACAGATAAGTGCAGGGTTTATTAAAGCTGTCCGACAGACTTTCTTAACCTGGTAATATCTCTCTTGGGTGACTCTCCAAACATCCGCGAATATTCCCGACTGAAATGGGATAGCTTTCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAA  >  NC_000913/1590185‑1590675                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      catcgcctgagcgataccgtaggcgatggatagtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTaa                                                                                                                                                                                                                                                  <  2:7755‑M2/36‑1 (MQ=255)  atcgcctgagcgataccgtaggcgatggatagtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAAc                                                                                                                                                                                                                                                 <  1:599831‑M2/37‑1 (MQ=255)      cctgagggataccgtaggcgatggatagtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaagcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGAc                                                                                                                                                                                                                                             <  2:266609‑M2/41‑1 (MQ=255)                        cgatggatagtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCtgtggg                                                                                                                                                                                                                           >  1:201428‑M2/193‑250 (MQ=255)                          atggatagtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTgtggtg                                                                                                                                                                                                                          >  2:102217‑M2/191‑250 (MQ=255)                                agtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCg                                                                                                                                                                                                                    <  1:149014‑M2/66‑1 (MQ=255)                                 gtttgctggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCgtn                                                                                                                                                                                                                  <  2:325627‑M2/68‑2 (MQ=255)                                        ggcaacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGttca                                                                                                                                                                                                           <  1:395678‑M2/75‑1 (MQ=255)                                           aacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTa                                                                                                                                                                                                                  <  2:609124‑M2/68‑1 (MQ=255)                                           aacaccggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTa                                                                                                                                                                                                                  >  1:609124‑M2/174‑241 (MQ=255)                                              accggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGc                                                                                                                                                                                                     >  1:301301‑M2/171‑251 (MQ=255)                                                cggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattacaatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCAt                                                                                                                                                                                                   <  1:90952‑M2/83‑1 (MQ=255)                                                 ggttaccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATc                                                                                                                                                                                                  >  2:402074‑M2/168‑251 (MQ=255)                                                     accagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTc                                                                                                                                                                                              <  1:531650‑M2/88‑1 (MQ=255)                                                      ccagaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCg                                                                                                                                                                                             >  1:109371‑M2/163‑251 (MQ=255)                                                        ngaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGcc                                                                                                                                                                                           >  2:308899‑M2/161‑251 (MQ=255)                                                         gaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCt                                                                                                                                                                                          >  1:468378‑M2/160‑251 (MQ=255)                                                         gaatgcgcttaccggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCt                                                                                                                                                                                          >  1:578639‑M2/160‑251 (MQ=255)                                                                cttaccggaaagcaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTc                                                                                                                                                                                    >  1:488564‑M2/153‑250 (MQ=255)                                                                   accggaaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCAtt                                                                                                                                                                                 <  2:661283‑M2/101‑1 (MQ=255)                                                                        aaagaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACg                                                                                                                                                                           >  1:432789‑M2/145‑251 (MQ=255)                                                                           gaaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAg                                                                                                                                                                        <  2:335187‑M2/110‑1 (MQ=255)                                                                            aaaacccatagctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGc                                                                                                                                                                       <  2:686647‑M2/111‑1 (MQ=255)                                                                                    tagctttaatccttattgttgatgcttgttgtgcctgacaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTctt                                                                                                                                                               >  2:525305‑M2/133‑250 (MQ=255)                                                                                     agctttaatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTg                                                                                                                                                               <  1:402074‑M2/119‑1 (MQ=255)                                                                                          taatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGttcattc                                                                                                                                                                                                        >  1:504732‑M2/127‑204 (MQ=255)                                                                                          taatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTAATACCg                                                                                                                                                         >  2:86060‑M2/127‑251 (MQ=255)                                                                                          taatccttattgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACAGCGTAATGttcattc                                                                                                                                                                                                        <  2:504732‑M2/78‑1 (MQ=255)                                                                                                  attgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACt                                                                                                                                                                                      <  1:90346‑M2/96‑1 (MQ=255)                                                                                                  attgttgatgcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACt                                                                                                                                                                                      >  2:90346‑M2/119‑214 (MQ=255)                                                                                                           gcttgttgtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCg                                                                                                                                        >  2:239734‑M2/110‑251 (MQ=255)                                                                                                                  gtgcctgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGAt                                                                                                                                  <  2:201428‑M2/148‑1 (MQ=255)                                                                                                                       tgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCAtt                                                                                                                                                                   >  1:559791‑M2/98‑212 (MQ=255)                                                                                                                       tgaaaatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCAtt                                                                                                                                                                   <  2:559791‑M2/115‑1 (MQ=255)                                                                                                                           aatcaggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTa                                                                                                                         >  1:486219‑M2/94‑250 (MQ=255)                                                                                                                              caggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAAt                                                                                                                      >  2:666205‑M2/91‑250 (MQ=255)                                                                                                                               aggcgattcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATa                                                                                                                     >  1:208534‑M2/90‑250 (MQ=255)                                                                                                                                    attcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATcc                                                                                                                                >  1:401219‑M2/85‑234 (MQ=255)                                                                                                                                    attcgtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATcc                                                                                                                                <  2:401219‑M2/150‑1 (MQ=255)                                                                                                                                        gtcgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGCATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGc                                                                                                            >  2:547167‑M2/81‑250 (MQ=255)                                                                                                                                          cgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCAcc                                                                                                         >  1:613888‑M2/79‑251 (MQ=255)                                                                                                                                          cgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCAcc                                                                                                         >  2:620608‑M2/79‑251 (MQ=255)                                                                                                                                          cgttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCAcc                                                                                                         <  2:488564‑M2/173‑1 (MQ=255)                                                                                                                                           gttttagtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCAccc                                                                                                        <  1:86060‑M2/174‑1 (MQ=255)                                                                                                                                             tttagtcaacagtacgaacagataaccggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGAATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGACGCCTGCCTTCATTGAGACGTAGCCATTTCTTCTATCGGAGTGGAATCATTCCAGCTTGCTCCGTCCTTATATAATGAAAAGCACCct                                                                                                       >  2:454499‑M2/76‑250 (MQ=255)                                                                                                                                                 gtaaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGgt                                                                                                  <  1:666205‑M2/180‑1 (MQ=255)                                                                                                                                                   aaacagtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGgtgt                                                                                                >  1:639963‑M2/70‑251 (MQ=255)                                                                                                                                                    aacagtacgaacagataaacgattattataatcaacctggctgtgagtagctatagttgccaggtccgCAAATATTCCCGACTAAAATGTGATAGCTTCCGTAACCGACATCATAGTCTGCTGTAGTGACATCGTAATTTTCATTCAGCATCTGTCGCCTGACTTCATTTAGACTTACCCATTTCTGATACCGGATTTGACTCATTCCCGCTTGATCCGTCCTTAAATAAATAACagcaccctcttatgtn                                                                                               <  2:312907‑M2/183‑7 (MQ=255)                                                                                                                                                       agtacgaacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTc                                                                                            <  2:301301‑M2/186‑1 (MQ=255)                                                                                                                                                           cgaccagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTAt                                                                                        <  2:116919‑M2/190‑1 (MQ=255)                                                                                                                                                             aacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGc                                                                                      >  1:327282‑M2/60‑251 (MQ=255)                                                                                                                                                             aacagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGc                                                                                      >  1:39346‑M2/60‑251 (MQ=255)                                                                                                                                                               cagataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGc                                                                                      >  2:366531‑M2/58‑249 (MQ=255)                                                                                                                                                                 gataaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAgtg                                                                                  <  2:486219‑M2/196‑1 (MQ=255)                                                                                                                                                                    aaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTgaga                                                                                                                                                                             >  1:4000‑M2/53‑157 (MQ=255)                                                                                                                                                                    aaacggttattataatcaacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTgaga                                                                                                                                                                             <  2:4000‑M2/105‑1 (MQ=255)                                                                                                                                                                               ataatcaacctggctgtgagtagatatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCgacagac                                                                    >  1:211950‑M2/42‑251 (MQ=255)                                                                                                                                                                                    caacctggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTgc                                                               >  1:76542‑M2/37‑251 (MQ=255)                                                                                                                                                                                          ggctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGctct                                                          <  1:274103‑M2/220‑1 (MQ=255)                                                                                                                                                                                           gctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCctc                                                                                                      >  2:82270‑M2/30‑205 (MQ=255)                                                                                                                                                                                           gctgtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCctc                                                                                                      <  1:82270‑M2/176‑1 (MQ=255)                                                                                                                                                                                              gtgagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCa                                                     <  2:48329‑M2/225‑1 (MQ=255)                                                                                                                                                                                                gagtagctatagttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATg                                                   >  2:126676‑M2/25‑251 (MQ=255)                                                                                                                                                                                                           gttgccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCGTCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAAt                                        >  1:69950‑M2/14‑251 (MQ=255)                                                                                                                                                                                                              gccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTccc                                                                                                                                         >  1:132922‑M2/11‑151 (MQ=255)                                                                                                                                                                                                              gccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTccc                                                                                                                                         <  2:132922‑M2/141‑1 (MQ=255)                                                                                                                                                                                                              gccaggtccgCGAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATaa                                      <  2:109371‑M2/240‑1 (MQ=255)                                                                                                                                                                                                               ccaggtccgCGAATATTCCCGGCTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGAGCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGGTTTCGTATGCAGTGTCTATGCGACAGACACTGCGTTCTTAGCATGGGGCAGGGAATAAcc                                    <  2:211950‑M2/242‑1 (MQ=255)                                                                                                                                                                                                                      cgcgAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTAt                             <  1:102217/251‑1 (MQ=255)                                                                                                                                                                                                                        cgAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAAGAACCAGGCTAttt                           <  1:525305/251‑1 (MQ=255)                                                                                                                                                                                                                         gAATATTCCCGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTTCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtc                          >  1:669899/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccGACTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGc                   >  1:235646/1‑250 (MQ=255)                                                                                                                                                                                                                                    aCTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtct                         >  2:198620/1‑241 (MQ=255)                                                                                                                                                                                                                                    aCTGAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTtct                         <  1:198620/241‑1 (MQ=255)                                                                                                                                                                                                                                       gAAATGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGc             <  1:239734/250‑1 (MQ=255)                                                                                                                                                                                                                                        aaaTGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGcc            >  2:351199/1‑250 (MQ=255)                                                                                                                                                                                                                                         aaTGGGATAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCaa          <  1:308899/251‑1 (MQ=255)                                                                                                                                                                                                                                               aTAGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGa    <  1:126676/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaaa  <  2:432789/251‑1 (MQ=255)                                                                                                                                                                                                                                                 aGCTTCCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGaa   >  2:641826/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CTAAACAGAATATTATTGCGAACAAGGGAAGTGTCAGGAAAGGTCAAGAGCTACCAATGGTTAATATGGCTATAAATCATTAGCTATGGTTAATGTTTGCTTGCTGAAACTAACACCGAATTGCTCTAAACAGATAAGTGCAGGGTTTATTAAAGCTGTCCGACAGACTTTCTTAACCTGGTAATATCTCTCTTGGGTGACTCTCCAAACATCCGCGAATATTCCCGACTGAAATGGGATAGCTTTCGTAACCGACAGCATAGGCTGCTGTGGTGACATCGTAATGTTCATTCAGCATCTGTCGCCTGACTTCATTGAGACGTAGCCATTTCTGATACCGGAGTGGACTCATTCCCGCTTGATCCGTCCTTAAATAAGTAACAGCACCCTCTGGTGTTTTCGTATGCAGTGTCTATGCGACAGACACTGCGCTCTTAGCATGGGGCAGGGAATAACCAGGCTATTTCTCCATGCACAGGCCAATTATGAAA  >  NC_000913/1590185‑1590675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑