breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 38 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,228,2780GC43.8% 34.3 / 11.7 32intergenic (‑1008/‑704)yohF/yohPputative oxidoreductase YohF/putative membrane protein YohP
*NC_000913811,7210TG43.4% 83.7 / 16.0 83V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_0009134,256,0690GC34.0% 92.3 / 15.9 50P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase
*NC_0009131,101,6730TA34.0% 95.3 / 14.8 53L4F (TTA→TTTcsgGcurli secretion channel
*NC_0009132,326,4500TG33.8% 115.2 / 18.0 77G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009132,839,3590TG32.4% 127.8 / 12.4 72intergenic (‑95/‑165)ascG/ascFDNA‑binding transcriptional repressor AscG/beta‑glucoside specific PTS enzyme IIBC component
*NC_0009131,101,6760GA29.6% 91.2 / 13.3 55R3R (CGC→CGTcsgGcurli secretion channel
*NC_0009132,962,4950TG29.5% 137.0 / 10.1 95S90S (TCA→TCCppdCprepilin‑type N‑terminal cleavage/methylation domain‑containing protein PpdC
*NC_0009134,171,5570CG29.4% 72.2 / 53.0 52intergenic (+13/‑80)rrlB/rrfB23S ribosomal RNA/5S ribosomal RNA
*NC_0009134,201,2780AC28.9% 143.2 / 10.3 84G137G (GGT→GGGzraPsignaling pathway modulator ZraP
*NC_000913463,2460TG28.3% 159.7 / 11.1 93G444G (GGT→GGGppiDperiplasmic folding chaperone
*NC_0009131,579,7570CA28.2% 52.9 / 11.8 39C345F (TGC→TTC) ydeMputative anaerobic sulfatase maturation enzyme YdeM
*NC_0009134,296,2680TC27.6% 40.4 / 17.4 29intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,788,0250TG27.5% 195.3 / 11.2 103G396G (GGT→GGGenvCmurein hydrolase activator EnvC
*NC_000913949,1180CT27.3% 108.9 / 18.2 66E151K (GAA→AAA) ycaNputative DNA‑binding transcriptional regulator YcaN
*NC_0009132,336,5940AC27.0% 138.0 / 11.6 75G17G (GGT→GGGyfaADUF2138 domain‑containing protein YfaA
*NC_000913647,2100AC26.8% 180.9 / 11.1 100G100G (GGT→GGGcitGtriphosphoribosyl‑dephospho‑CoA synthase
*NC_0009134,213,7650AC26.8% 132.3 / 13.7 71V120G (GTG→GGG) yjaBpeptidyl‑lysine N‑acetyltransferase YjaB
*NC_000913937,9420AC26.3% 147.1 / 10.9 76T191P (ACC→CCC) lolAouter membrane lipoprotein carrier protein
*NC_0009131,876,0150AG26.2% 179.6 / 12.8 83I115V (ATT→GTT) nimT2‑nitroimidazole exporter

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 993094 =97 (1.080)3 (0.030) 3/480 6.6 3.4% coding (2473/2613 nt) pepN aminopeptidase N
?NC_000913 1726646 = 77 (0.880)intergenic (+24/‑13) nemR/nemA DNA‑binding transcriptional repressor NemR/N‑ethylmaleimide reductase
* ? NC_000913 2929131 =138 (1.540)3 (0.030) 3/492 6.7 2.6% coding (903/1290 nt) sdaC L‑serine:H(+) symporter SdaC
?NC_000913 = 3511404 85 (0.950)coding (19/363 nt) yhfY PRD domain‑containing protein YhfY