| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,171,557 | 0 | C | G | 29.4% | 72.2 / 53.0 | 52 | intergenic (+13/‑80) | rrlB/rrfB | 23S ribosomal RNA/5S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (14/22); new base G (5/10); total (19/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.95e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACGAAACTTGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTT > NC_000913/4171401‑4171801 | aCGAAACTTGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTa < 1:338326/251‑1 (MQ=31) gAAACTTTCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAAGTTGCCTGGCGGCAGTAgc < 2:278271/251‑1 (MQ=14) aaaCTTGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGCGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAgcg > 2:409098/1‑251 (MQ=14) tGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGAAGAAGCGCgg < 2:164916/249‑1 (MQ=14) ccccGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCggtggt < 2:77287/251‑1 (MQ=31) tctcCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAccc < 1:408625/250‑1 (MQ=31) gACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGATTGAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGcc < 1:266577/250‑1 (MQ=14) gACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGcc < 1:390557/249‑1 (MQ=31) ccccTTTAAGGGTCCTGAAGGAACGTAGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATGTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGaa < 2:60681/250‑1 (MQ=14) tttAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTc > 1:704544/1‑250 (MQ=31) tAAGGGTCCTGAAGGAACATTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAg < 1:363318/250‑1 (MQ=14) aaGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGaa < 1:475371/251‑1 (MQ=31) ccTGAAGGAACGTTGAAGACGACGACGTTGNAAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGaa < 2:360661/250‑1 (MQ=11) aaCGTTGAAGACGACGACGTTGATAGGCCGGGTGTAGAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAg < 2:91854/251‑1 (MQ=11) tGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGcc < 1:651687/250‑1 (MQ=31) gacgacgacGTTGAGAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAGCCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTTGCGCCGATgg < 2:274914/251‑1 (MQ=14) gacgacgTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAg > 2:62927/1‑188 (MQ=32) gacgacgTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAg < 1:62927/188‑1 (MQ=32) acgacgTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTCCAACGCCGAAGCTGTGTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAgt < 2:167973/251‑1 (MQ=14) acgTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGtg‑tg < 1:184235/251‑1 (MQ=11) acgTTGATAGGCAGGGTGTGTAAGAGCAGCGATGCGTTGAGCTCACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGCAGCTGTTTTGGCGGAT‑GAGAGAAGAGTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTCGCGGCAGTAACGCGGGGGTCACACCTGACCCCATGCCGCACGCCGGAGGGAAAGGCCGCAGCGCCGGTggaaggggg > 2:721195/1‑244 (MQ=11) ttGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑Tgggg > 1:518486/1‑251 (MQ=14) aGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATTAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTGGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGtctc < 2:291131/250‑1 (MQ=14) aGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGtctc < 1:67947/250‑1 (MQ=31) ggCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCAGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTccc < 1:399475/251‑1 (MQ=14) cGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCAt < 1:118501/251‑1 (MQ=31) ggTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGc < 2:224545/251‑1 (MQ=11) tgtAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACATTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGGAGCGACGATGGTAGGG‑GGGGGTCTCCCCATgaga > 2:382228/1‑246 (MQ=11) gtAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCgag > 2:562666/1‑249 (MQ=11) gcAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGa < 1:93464/251‑1 (MQ=11) gcAGCGATGAGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTGTGGCGGAT‑GAGAGAAGATGTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGa < 2:396281/251‑1 (MQ=2) agagATGAGTTGAGCTAAACGGTAATAATGAACCGCGAGGCTTAACCTTACAACGCCGACGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAAAGCAAAAGCGGTCTGAATAAAA‑AGAATTAGCCTTGCGGCAGTAGCGCGGTGGACCCACCTGACCCCATGCCGAACTCAGAAGGGAAAGGCCGTAGCGCCGATGGTGGGGGTGGGG‑CTCACCATGCGAGAGTAGGGaa > 2:594414/4‑250 (MQ=11) cGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTg < 1:586462/251‑1 (MQ=31) gCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGAACCCATGCCGAACTCAGAAGTGAAACGACGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGcc > 1:32652/1‑249 (MQ=11) cGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCGGACCCCATGCCGAGCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAgg < 1:89065/251‑1 (MQ=11) gTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGc > 1:537836/1‑251 (MQ=11) gTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCACCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGc < 1:398118/251‑1 (MQ=11) gAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATc < 1:432187/251‑1 (MQ=11) gAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCAt < 1:217438/250‑1 (MQ=31) gCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGGCCCAACTGCCCCCATGCCGAACTCAGAAGGGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGAAGGGAACGGCCCGGCATCa > 2:164167/1‑250 (MQ=11) aaCCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAAt > 1:436668/1‑250 (MQ=31) ttACTAATGACCCGGGAGGCTTAACCTTACAACGCCGAAGCTGGTGTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACCGATTAAATCAAAACGCAGAAGCGGTCTGA‑TAAAACAGGATTTGCCTGGCGGCAGTAGAGGGGAGATCCCCCCTGACCCCATGCCGAACTCCGAAGGGAAACGCCGTTGAGCAGCTGGTAGTG‑TGGGGCCTCCCCATACGAGAACAGGGACAGGCAAGGCATCAAATAAAACg > 2:475258/2‑251 (MQ=2) cTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGaa > 2:527432/1‑250 (MQ=35) aaTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGAG‑TGGGGTCCCCCCATGCGAGAGTAGGGAACTGCCCGGCATCAAATAAAACGAAAgg > 2:385914/1‑251 (MQ=25) aaTGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGAGTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAgg < 2:498435/251‑1 (MQ=25) aGGCTTAACCTTACAACGCCGAAGATGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGaaa < 1:409098/251‑1 (MQ=21) ccTTACAACGCCGAAGCTGTCTTGGCGGAT‑GAGAGAAGATTTTCAGACTGATACAGCTTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGGCCCCATGCCGAACTCAGAAGTGAAACGCCGTAGAGCCTATGGTAGTG‑TGGGGGCTCCTCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCGCAGTCTGAAGACTGGGc < 2:417613/251‑1 (MQ=11) cAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCGTTc > 1:361705/1‑251 (MQ=37) gCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGt > 1:217267/1‑190 (MQ=11) gCCGAAGGTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGt < 2:217267/190‑1 (MQ=11) gCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGCCTGGGCCTTTCGttt > 1:536940/1‑251 (MQ=37) cGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAACCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGACCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAACGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCCTCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGtttt > 1:32643/1‑250 (MQ=255) | ACGAAACTTGCCCCGAGATGAGTTCTCCCTGACCCTTTAAGGGTCCTGAAGGAACGTTGAAGACGACGACGTTGATAGGCCGGGTGTGTAAGCGCAGCGATGCGTTGAGCTAACCGGTACTAATGAACCGTGAGGCTTAACCTTACAACGCCGAAGCTGTTTTGGCGGAT‑GAGAGAAGATTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGA‑TAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTG‑TGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTT > NC_000913/4171401‑4171801 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |