Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913463,2460TG28.3% 159.7 / 11.1 93G444G (GGT→GGG) ppiDperiplasmic folding chaperone
Reads supporting (aligned to +/- strand):  ref base T (25/41);  new base G (24/2);  total (50/43)
Fisher's exact test for biased strand distribution p-value = 1.39e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTT  >  NC_000913/462999‑463492
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                              
aaTCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGa                                                                                                                                                                                                                                                              >  1:495459/1‑250 (MQ=255)
   cGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAAc                                                                                                                                                                                                                                                          <  1:400252/251‑1 (MQ=255)
   cGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAAc                                                                                                                                                                                                                                                          <  1:667342/251‑1 (MQ=255)
    gTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGaaaa                                                                                                                                                                                                                                                           >  1:69124/1‑249 (MQ=255)
    gTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGaaaa                                                                                                                                                                                                                                                           >  1:521204/1‑249 (MQ=255)
     ttAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGGAAACgg                                                                                                                                                                                                                                                        >  1:264379/1‑251 (MQ=255)
       aGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGgc                                                                                                                                                                                                                                                       <  1:169993/250‑1 (MQ=255)
       aGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGTGAAAACGgcg                                                                                                                                                                                                                                                      >  1:573808/1‑251 (MQ=255)
       aGACGAAGTACGGTACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGgcg                                                                                                                                                                                                                                                      <  2:698160/251‑1 (MQ=255)
            aaGTACGTGACGACATTGCGGCGAAAGTGAACCACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATATTTAACGGCGGGCT‑GGTAGGGGGAAAAGGGGCGCCg                                                                                                                                                                                                                                                 >  2:293075/1‑251 (MQ=255)
                  gTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGGCGCTATCTTTAACGGCGGTCT‑GGGAGGGGAAAACGGCGCGCCGGGCATc                                                                                                                                                                                                                                           >  1:248188/1‑251 (MQ=255)
                    gacgacATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGGAAACGGCGCGCCGGGGATC‑a                                                                                                                                                                                                                                         >  1:210404/1‑250 (MQ=255)
                     acgacATTGCGGCGAAAGTGAAACCCGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGCCGCTATCTTTAACGGCGGTCT‑GGTGGGGGCAAACGGCGCGCCCGGCATctc                                                                                                                                                                                                                                         >  2:182088/1‑248 (MQ=255)
                     acgacATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCACC‑AAc                                                                                                                                                                                                                                       >  1:6038/1‑251 (MQ=255)
                       gacATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑TGGAGGTGCAAACGGGGCGCCggcccccaaccc                                                                                                                                                                                                                                      >  1:158845/1‑241 (MQ=255)
                            tGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AAATCTGAc                                                                                                                                                                                                                                 >  1:111968/1‑250 (MQ=255)
                            tGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGAGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGCTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGGCTTGGTAGGGGAAACCGGGGCGC‑GGGGATC‑AAatctcac                                                                                                                                                                                                                                 >  2:479470/1‑247 (MQ=255)
                                gcgAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGTGAAAACGGCGCGCCGGGCCAC‑AACTCTGAcaacct                                                                                                                                                                                                                            >  1:674457/1‑247 (MQ=255)
                                  gAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGAAGCAATAAGAGAGCGATGCGGCAAGCAACGACACCGAATCTCTGGCAGGAGCAGAGCAAGCTGACGGCGTTAAAGCCACTCAGACGGGGTGGTTCAGCACAGATAACATGACAGAAAAGGTGAACTTCAAGCCGGTTGCCGAAGAAATCTTTAACGGCGGGCT‑GGGAGGGGAAAAAGGCGCCACGGGCATC‑AActctagcaccctc                                                                                                                                                                                                                           >  2:53940/1‑241 (MQ=255)
                                    aaGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGGCT‑GGGAGGGGGAAACGGGGAGCCGGGCACC‑AAACCTGAcactaccccc                                                                                                                                                                                                                        >  2:168656/1‑243 (MQ=255)
                                      gTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGGGAAAACGGGGCGCCGGGCCTT‑AACCCTGAcatcacccacgt                                                                                                                                                                                                                      >  2:72760/1‑244 (MQ=255)
                                      gTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGt                                                                                                                                                                                                                      >  1:340578/1‑251 (MQ=255)
                                       tGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAAACGCGCGCCGGGGATC‑AAATCTGAcatcatacacgt                                                                                                                                                                                                                      >  1:721196/1‑244 (MQ=255)
                                        gAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGAAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGACGCGCCGGGGCTC‑AACACTGTCATCATCAcccga                                                                                                                                                                                                                     >  1:233069/1‑247 (MQ=255)
                                        aaaaCACGAAAAAGCCCTCGATGCGTACTACNCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGGGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAg                                                                                                                                                                                                                    <  2:360362/250‑1 (MQ=255)
                                         acacacGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGTGTAAAAGGGGCGCCGGGCATCCAActctacaacatccccggga                                                                                                                                                                                                                     >  2:65506/3‑235 (MQ=255)
                                                       ccTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTAAAGCCGGTTGCCGACGCTATCTTTAACGGCGGGCT‑GGTAGGTGGAAAAGGGGCGCCGGGCGTC‑AACTCTGGCATACTCACCGTAAAAGGCGACCgcgcc                                                                                                                                                                                                      >  2:426246/1‑249 (MQ=255)
                                                              gCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGtgct                                                                                                                                                                                              <  1:658517/251‑1 (MQ=255)
                                                                gTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTTACATCATCAACGTAGACGGCGACCGCGCATTCGtgctgc                                                                                                                                                                                            >  1:21067/1‑251 (MQ=255)
                                                                 tactacGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑TGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCCGACATCATACCCGTAGACGGCGAACGCGCATTCGtgtggcg                                                                                                                                                                                           >  1:3960/1‑246 (MQ=255)
                                                                 tactacGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGGGAAAACAGCGCGCCCGGCCTC‑AAACCTGCCATCATCCCCGGAGACGGCGGCCGCGCCTTCGTGCTgcg                                                                                                                                                                                           >  2:578663/1‑251 (MQ=255)
                                                                            gcagcaGAAAGGGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGGCGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTATACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGc                                                                                                                                                                                <  2:248188/251‑1 (MQ=255)
                                                                                cagAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACATGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGGAAACGGCGCGGCGGGCATC‑AACTCTGACATCATCACCGGAGACGGCGGACGGGCATTCGTGCTGGGCAACAGCggggaca                                                                                                                                                                             >  1:227777/1‑244 (MQ=255)
                                                                                      gTGAGCGATGCGGCCAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAACGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGGAACCGGCGGGCCGGGCATC‑AAACCTGACCTCATCACCCTAGACGGCGACCCCGCATTAGTGCTGCCCCCCAGCCAGCC‑CAAACCggg                                                                                                                                                                     >  2:476394/1‑250 (MQ=255)
                                                                                       ggAGCGATGCGGCAGGCAACGACGCCGAGGCTCTGGCCGGTGCAGAGCAAGCTGCCGGAGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGaa                                                                                                                                                                    <  1:159295/250‑1 (MQ=255)
                                                                                       ggAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGaa                                                                                                                                                                    <  1:73693/250‑1 (MQ=255)
                                                                                         aGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTTGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGc                                                                                                                                                                  <  1:266614/251‑1 (MQ=255)
                                                                                          gCGATGCGGCAAGCAACGACCCCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATTACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGc                                                                                                                                                                  <  1:286286/250‑1 (MQ=255)
                                                                                             aTGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGGAAAAGGCGCGCCGGGCCTC‑AACTCTGACATCATCACCGTAGACGGCGGCCGCGCATTCGTGCTGCGCATCAGCGGGCAACAAAC‑GGAAGCgggg                                                                                                                                                              >  1:634841/1‑249 (MQ=255)
                                                                                             aTGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTg                                                                                                                                                              <  1:159989/251‑1 (MQ=255)
                                                                                                    aagcaaCGACACCGAGTCTCGGGCCGGTGCAGAGCAAGCTGCCGGCGTGAAAGCCACTCATGCGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTGGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGt                                                                                                                                                      <  2:167591/251‑1 (MQ=255)
                                                                                                      gcaaCGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCC‑GGTAGAGGAAAAAGGCGCGCCGGGCATC‑AAATCAGACATAATCCACGGAGACGGCCACAGCGCCATCGTGCCACGCCTCAGCGAGCA‑CCAAACGGAAGCgggggaacccttg                                                                                                                                                     >  1:530544/1‑240 (MQ=255)
                                                                                                           gACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCATC‑AACTATGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGGAGCGGGGAAA‑CCGCTGGc                                                                                                                                                  >  1:478211/1‑248 (MQ=255)
                                                                                                             ccccggatcgctGGCCGGGGCAGAGCAAGCTNCCGGCGTTAAAGCCACGCAGACGGGTTGGTTCAGCAAAGATAACCGGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑Tg                                                                                                                                            <  2:325251/241‑1 (MQ=255)
                                                                                                              cccgggTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTGAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGt                                                                                                                                           <  1:325185/246‑1 (MQ=255)
                                                                                                              accggctttctgccaggcgaagagCAAGCTGCCTGCGTTAAAGCGCCTCAGACGGGTGGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTGTAAGGCGGGTGCCGTCGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGCCGCGCCGGGCATC‑AACGCTGACATCATCTCCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGt                                                                                                                                           <  2:705544/231‑1 (MQ=255)
                                                                                                                cgtgTCTCGGGCCGGGGAAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCGTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTc                                                                                                                                         <  2:165316/248‑1 (MQ=255)
                                                                                                                  aggcgctGGCCGGTGCAGAGCAAACTGCCGGCGGTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCAGCACCGTAGACGGCGACCGCGCATGCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAg                                                                                                                                       <  2:158845/246‑1 (MQ=255)
                                                                                                                          gCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGt                                                                                                                               <  1:259925/251‑1 (MQ=255)
                                                                                                                             ggTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGGAGGCGACCGCGCAATCGTGCTGCGCATCAGCGGGCA‑CAAACCGGAAACGGTGGAA‑ACGGTGGGAGA‑TGGTCAGGAAAAAggta                                                                                                                             >  1:161570/1‑247 (MQ=255)
                                                                                                                                gCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGc                                                                                                                         <  2:163522/251‑1 (MQ=255)
                                                                                                                                      cAAGCTGCCGGCGTTAAAGCCGCTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGATCTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAATCCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGt                                                                                                                  <  1:474221/251‑1 (MQ=255)
                                                                                                                                       aaGCTGCCGGCGTTCAAGCCACTCAGACGGGTTGGTTCAGCAAAGATACCCTGCCGGAAGAGTTTAACTTCAAGCCTGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAATCCGGAAGCTGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGtt                                                                                                                 <  2:396864/251‑1 (MQ=255)
                                                                                                                                       aaGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑TGTAGGGGAAAACGGCGCGCCCGGCCTC‑AACTCTGACATCATCACCCTAAAAAGCGACCCCGCCTTCGTGCTGCGCATCAGCGCGCC‑CAAAACGGAAACCGCGAAA‑CCGCTGGCAAA‑AGTTCAGGAACAAATTAAGGC‑ATTGGtt                                                                                                                 >  1:618322/1‑251 (MQ=255)
                                                                                                                                       aaGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGtt                                                                                                                 <  1:641399/251‑1 (MQ=255)
                                                                                                                                       aaGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AAATCTGACATCATCACCGTAGACCGCGACCGCGCATTCGTGCTGCGCCTCAGCGAGCA‑CAAAACGGAAACGGGGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGtt                                                                                                                 >  1:311611/1‑251 (MQ=255)
                                                                                                                                               ggCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGGTGCCGACGCTATCTTTAACGGCGGTCT‑TGTAGGGGGAAACGGCGCGCCGGGTCTC‑AAAACTGGAATCATCACCGGAGACGGCGACCGCGCATTTGTGCTGCGCAGCAGCGAGCC‑AAAACCGGCAGAGGGGAAA‑CCGTGGGGAGACTGT‑GAGGAACAAGTTAAGGC‑AATGGttaaacaac                                                                                                          >  1:43802/1‑243 (MQ=255)
                                                                                                                                                      aaGCCCCTCAGCCGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGGTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGcg                                                                                                 <  2:69124/251‑2 (MQ=255)
                                                                                                                                                            cTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGaa                                                                                                                                                                    <  2:705329/182‑1 (MQ=255)
                                                                                                                                                              cAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGaacaa                                                                                           <  1:531642/249‑1 (MQ=255)
                                                                                                                                                              cAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGACT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGaacaac                                                                                          <  1:493346/250‑1 (MQ=255)
                                                                                                                                                                       tctttggtctcaGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCAGCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGACCAACAGGCG‑AAAg                                                                                <  2:577859/239‑1 (MQ=255)
                                                                                                                                                                            cAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGACT‑GGTAGGGGAAAACGGCGCGCCCGGCATA‑AACTCTGACATCATCACCGGAGACGGCGACCGCGCATTCATGCTGCGCACCCGCGAGCA‑CAAACCCGAAGGGGTGAAA‑ACGGTGGCCGG‑TGTTCAGGAACAAAGTAAGGC‑ATTGGTT‑CAGCAAAAACAAGCTGAACAACAGGCG‑AAAATGGATg                                                                          >  1:474/1‑251 (MQ=255)
                                                                                                                                                                                aaaGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGGCGGGGATC‑AAATCTGACATCATCCCCGTAGACGGCGGCCGCGCAATCGGGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGGGAAA‑CCGGTGGCCGA‑TGGTCAGGAAACAGGTAAGGCCATTG‑TT‑TAGCACAAAAAAAACGAACAAAAGGCG‑AAAGTgggggatg                                                                       >  1:196318/1‑244 (MQ=255)
                                                                                                                                                                                 aaGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTg‑ag                                                                    <  1:417082/251‑1 (MQ=255)
                                                                                                                                                                                  aGATAACCTGCCGGAAGAGTTGAGCTTCAAGCCGGTTGCCGACGCTATCGTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGGGGATGCTg‑aga                                                                   <  2:38682/251‑1 (MQ=255)
                                                                                                                                                                                         cTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGGCGGCGAACGCGCATTCGTGCTGCGCATCAGCGGGAA‑CAAACCGGAAGCGGTGAAA‑ACGTTGGGAGA‑TGTTTAGGAACAAGGTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGAAGCTG‑AGaaaatgac                                                            >  1:478470/1‑246 (MQ=255)
                                                                                                                                                                                           gCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTTT‑GGGGGGTGGAAAAGGGGCGCCGGGCAAC‑CACTCCGACAACATAACCGGCGACGGCGACCGCGCATTCGTGCTGCGCATCCGCGAGCA‑CAAACCGGAAGCGGAGAAAACCGT‑GGCCGA‑TGTTCAGGAACAAGATAAGGC‑ATTGGTT‑TAGCACAACAAAGTGGAACAACCGGCGCAAAG‑GGGTGCTg‑aggaaatgcggg                                                          >  1:323315/1‑241 (MQ=255)
                                                                                                                                                                                           gCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGGCT‑GGTAGGTGAAAACGGCCCGCCGGGCATC‑AAAACTGTCATCCTCAACGCAGACGGCGGCCGCCCATACGAGCTGCGCAAACGCGAGAC‑CAAACCGGAAGGGGGGAAA‑CCGGTAGCAAC‑TGCCCAGGGACAAGTTAAAGC‑AATGGGT‑CCGCCCAAAAAAGCTGGACANAAGGCggacgagcacactgcgaaaacgctgg                                                            >  2:370937/1‑226 (MQ=255)
                                                                                                                                                                                            ccGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGTAAAAGGCGCGCCGGGCATC‑AACTCTTACATCCTCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAACGGTGAAA‑ACGTTGGCAGA‑TGTTCAGGAACAAGGTAAGGC‑ATTGGGT‑CAGCACAACAAAGATGAACAACAAGCG‑AAAGAGGATGCTT‑AGAAACTGCTGGt                                                         >  1:441181/1‑251 (MQ=255)
                                                                                                                                                                                               gAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCATC‑AACTCTGAAATCCTCACCCTAGACGGCGACCGCGCATTCGTGCTTCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGATAAGGC‑ATTGGTT‑CAGCACAACAAAACTGAAAAAACGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTg                                                       >  1:27066/1‑250 (MQ=255)
                                                                                                                                                                                                  gagTTGAACTTCAAGCCGGGTGCCTGCGCTATCTCTAACGGCGGGCG‑GGGAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑GGTTCAGGAACAAGTTAAGGC‑GTTGGTG‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGAttt                                                   <  2:76553/251‑1 (MQ=255)
                                                                                                                                                                                                  gagTGGAACTTCAAGCCGGTTGCCGACGAGAGCTTTAACGGCGGTCT‑GTTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGAttt                                                   <  2:512796/251‑1 (MQ=255)
                                                                                                                                                                                                       gAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGaaa                                               <  1:438937/250‑1 (MQ=255)
                                                                                                                                                                                                          cTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGGAGCGGGGAAA‑CCGGTGGCAGG‑TTTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAAGAG‑AAAGGGGATGCTG‑AGAAACTGCTGGTTGGGTTGAAAGCCg                                           >  2:85460/1‑251 (MQ=255)
                                                                                                                                                                                                            tCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCg                                           <  1:293075/249‑1 (MQ=255)
                                                                                                                                                                                                             cgaGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AAATCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTCGTTGATTTGAAAGCCGGc                                         <  2:614352/248‑1 (MQ=255)
                                                                                                                                                                                                             cAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCG‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCa                                        <  2:495459/251‑1 (MQ=255)
                                                                                                                                                                                                               ggCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGGGAAAACGGCGCGCCGGGCCTC‑AAATCTGACATCATCCCCGTAGACGGGGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCCGTGGGAGA‑TGGTCAGGAACAAGTTAAGGC‑AATGGTT‑CAGCACAACAAAGCTGAACAAAAGGCG‑GAAGTGGATGCTG‑AGAAAATGCTGGTTGATTTGAAAGCCGGCaa                                       >  2:581548/2‑250 (MQ=255)
                                                                                                                                                                                                                gCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAg                                     <  2:210404/251‑1 (MQ=255)
                                                                                                                                                                                                                  cGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGGAGGTGAAACCGGCCCGCCGGGCATC‑AAATCTGACATCATCACCGGAGACGGGGAACGCCCATTCGTGCATCGCATCAACGAGCA‑CAAAACGGAAGCGGTGAAA‑ACGTTGGCCGC‑TGGTCAGGAACAAGATAAGGG‑ATTGGTTTCA‑CACAAAAAAGATGAAAAACAAGGG‑AAAGTGGATGGTGGAAAAAC‑GCTGGGTGATTTTAAAGcagggcaaggg                                   >  2:181219/1‑241 (MQ=255)
                                                                                                                                                                                                                      tGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCAGC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCg                                <  2:573808/250‑1 (MQ=255)
                                                                                                                                                                                                                           aCGCTATCTTTAACGGCGGTCT‑GGGAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑GAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCt                          >  1:583658/1‑251 (MQ=255)
                                                                                                                                                                                                                             gCTATCTTTAACGGCGGGCT‑GGTAGGAGAAAACGGCGCGCCGGGGATC‑AACTCTGACATCATCCCCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGGGAAA‑CCGTTGGCAGA‑AGTTCAGGAACAAGTTAAGGG‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGAGGCGG‑CGAAAATGCTGGTTGGTTTTAAAACCGGCAAAAGGGCGGAAgaga                         >  2:511188/1‑247 (MQ=255)
                                                                                                                                                                                                                                    ttAACGGCGGTCT‑GGTGGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATGCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAgg                   <  2:63701/249‑1 (MQ=255)
                                                                                                                                                                                                                                       aCGGCGGTCT‑GGTAGGTGAAAACGGCGCTCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGcc              >  1:479882/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gcggTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGCGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGt           >  2:138201/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cggTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTc          >  1:430130/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cggTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTc          <  1:168656/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cggTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTc          >  1:623762/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  ggTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCATCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAAt    <  2:521204/251‑1 (MQ=255)
                                                                                                                                                                                                                                                  ggTAGGTGAAAACGGCGCGCCGGGCATC‑AACNCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAAt    <  2:352734/251‑1 (MQ=255)
                                                                                                                                                                                                                                                    gaGGTGAAAACGGCGCGCCGGGCATC‑AACTCNGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAAttt  <  2:340578/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                              
AATCGTTAGACGAAGTACGTGACGACATTGCGGCGAAAGTGAAACACGAAAAAGCCCTCGATGCGTACTACGCGCTGCAGCAGAAAGTGAGCGATGCGGCAAGCAACGACACCGAGTCTCTGGCCGGTGCAGAGCAAGCTGCCGGCGTTAAAGCCACTCAGACGGGTTGGTTCAGCAAAGATAACCTGCCGGAAGAGTTGAACTTCAAGCCGGTTGCCGACGCTATCTTTAACGGCGGTCT‑GGTAGGTGAAAACGGCGCGCCGGGCATC‑AACTCTGACATCATCACCGTAGACGGCGACCGCGCATTCGTGCTGCGCATCAGCGAGCA‑CAAACCGGAAGCGGTGAAA‑CCGTTGGCAGA‑TGTTCAGGAACAAGTTAAGGC‑ATTGGTT‑CAGCACAACAAAGCTGAACAACAGGCG‑AAAGTGGATGCTG‑AGAAACTGCTGGTTGATTTGAAAGCCGGCAAAGGTGCGGAAGCTATGCAGGCTGCCGGTCTGAAATTT  >  NC_000913/462999‑463492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑