breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,645 G→A W282* (TGG→TAG)  thrB → homoserine kinase
RA 71,170 T→C S262P (TCG→CCG)  araC → DNA‑binding transcriptional dual regulator AraC
RA 81,044 C→T L305L (CTG→CTA leuB ← 3‑isopropylmalate dehydrogenase
RA 81,102 G→A S286L (TCG→TTG)  leuB ← 3‑isopropylmalate dehydrogenase
RA 85,203 C→T P279L (CCG→CTG)  leuO → DNA‑binding transcriptional dual regulator LeuO
RA 154,182 C→T L415L (CTG→CTA htrE ← putative fimbrial usher protein HtrE
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 223,560 T→A intergenic (+152/‑211) gmhB → / → rrsH D‑glycero‑beta‑D‑manno‑heptose‑1,7‑bisphosphate 7‑phosphatase/16S ribosomal RNA
RA 246,985 C→T P92S (CCG→TCG)  yafL → NlpC/P60 family protein YafL
MC JC 253,287 Δ20,668 bp IS5‑mediated pepD[ykfC] 27 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 290,892 C→T M87I (ATG→ATA insB3 ← IS1 family protein InsB
RA 292,019 C→T L174L (CTA→TTA)  yagJ → protein YagJ
RA 309,381 C→T D216N (GAT→AAT)  ecpB ← putative fimbrial chaperone EcpB
RA 309,985 T→A G14G (GGA→GGT ecpB ← putative fimbrial chaperone EcpB
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
MC JC 392,967 Δ11,356 bp IS3‑mediated yaiT[dgcC] yaiT, iprA, ampH, sbmA, yaiW, yaiY, yaiZ, ddlA, iraP, phoA, psiF, [dgcC]
RA 410,666 C→T P175S (CCT→TCT)  mak → fructokinase
RA 421,027 C→T R14R (CGC→CGT proY → putative transporter ProY
RA 456,711 C→G P12R (CCC→CGC)  clpP → ATP‑dependent Clp protease proteolytic subunit
RA 472,849 A→G G84G (GGA→GGG glnK → nitrogen regulatory protein PII‑2
RA 495,622 C→T T168I (ACC→ATC)  htpG → chaperone protein HtpG
RA 534,742 G→A G276D (GGT→GAT)  gcl → glyoxylate carboligase
RA 547,891 C→T L404L (CTC→CTT fdrA → putative oxamate carbamoyltransferase FdrA
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 582,597 C→T intergenic (+500/‑278) tfaD → / → tfaX putative tail fiber assembly protein TfaD/protein TfaX
RA 694,941 G→A L5L (CTC→CTT miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
JC JC 771,310 IS1 (+) +9 bp intergenic (+699/‑140) mngB → / → cydA alpha‑mannosidase/cytochrome bd‑I subunit 1
RA 771,334 C→T intergenic (+723/‑124) mngB → / → cydA alpha‑mannosidase/cytochrome bd‑I subunit 1
RA 780,624 C→T noncoding (71/76 nt) lysT → tRNA‑Lys
RA 789,580 C→A E134* (GAA→TAA)  galK ← galactokinase
RA 790,832 Δ1 bp coding (198/1047 nt) galT ← galactose‑1‑phosphate uridylyltransferase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 884,892 C→T S407F (TCT→TTT)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 908,169 C→T R38R (AGG→AGA ybjT ← putative NAD(P)‑binding protein YbjT
RA 909,990 C→T E354K (GAG→AAG)  poxB ← pyruvate oxidase
RA 922,954 G→T A14A (GCG→GCT clpS → specificity factor for ClpA‑ClpP chaperone‑protease complex
RA 1,123,823 2 bp→AG coding (231‑232/648 nt) grxB ← reduced glutaredoxin 2
RA 1,147,792 T→A S58T (TCG→ACG)  plsX → putative phosphate acyltransferase
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC JC 1,300,894 IS1 (+) +8 bp intergenic (+347/‑281) insH21 → / → oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,317,162 G→A P21S (CCT→TCT)  trpA ← tryptophan synthase subunit alpha
MC JC 1,321,416 Δ1,470 bp coding (62‑1531/1563 nt) trpE ← anthranilate synthase subunit TrpE
RA 1,331,059 2 bp→AT coding (12‑13/2598 nt) topA → DNA topoisomerase 1
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,428,150 G→A L193L (CTG→TTG)  insH5 ← IS5 family transposase and trans‑activator
RA 1,489,491 G→A L420L (CTG→CTA aldA → aldehyde dehydrogenase A
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
JC JC 1,652,229 IS2 (–) +5 bp intergenic (+2244/+495) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,751,024 C→A intergenic (‑11/+53) ydhX ← / ← ydhW putative 4Fe‑4S ferredoxin‑like protein YdhX/uncharacterized protein YdhW
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,962,172 C→T A67A (GCC→GCT yecT → protein YecT
RA 1,996,243 G→A S197L (TCA→TTA)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,143,828 C→T P188L (CCA→CTA)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,188,087 T→C T110A (ACG→GCG)  yehA ← putative fimbrial adhesin YehA
RA 2,254,795 G→A A131V (GCG→GTG)  rihB ← pyrimidine‑specific ribonucleoside hydrolase RihB
RA 2,281,282 G→A Q217Q (CAG→CAA radD → putative DNA repair helicase RadD
RA 2,304,522 2 bp→TC intergenic (+129/+585) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,304,537 A→G intergenic (+144/+571) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,304,540 C→T intergenic (+147/+568) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,304,549 C→T intergenic (+156/+559) eco → / ← mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
RA 2,335,464 G→A A394V (GCG→GTG)  yfaA ← DUF2138 domain‑containing protein YfaA
RA 2,374,594 C→T C137Y (TGC→TAC)  menE ← o‑succinylbenzoate‑‑CoA ligase
RA 2,384,808 C→T K305K (AAG→AAA yfbK ← IPR002035/DUF3520 domain‑containing protein YfbK
RA 2,410,419 G→A L312L (CTG→TTG)  yfbS ← putative transporter YfbS
RA 2,417,352 G→A G91D (GGC→GAC)  yfcC → putative transporter YfcC
RA 2,444,820 C→T A247A (GCG→GCA yfcA ← putative transporter YfcA
RA 2,447,079 G→A A132V (GCC→GTC)  aroC ← chorismate synthase
RA 2,471,453 G→A T22M (ACG→ATG)  yfdK ← putative tail fiber assembly protein YfdK
RA 2,484,827 C→T L152F (CTT→TTT)  evgS → sensor histidine kinase EvgS
RA 2,522,742 G→A A415V (GCG→GTG)  xapB ← xanthosine:H(+) symporter XapB
RA 2,553,552 C→T R110W (CGG→TGG)  hemF → coproporphyrinogen III oxidase
RA 2,564,496 G→T intergenic (+124/‑27) yffR → / → yffS uncharacterized protein YffR/uncharacterized protein YffS
RA 2,566,734 G→A P51S (CCG→TCG)  eutA ← ethanolamine ammonia‑lyase reactivase EutA
RA 2,567,321 G→A P263S (CCT→TCT)  eutH ← putative ethanolamine permease EutH
RA 2,576,833 G→A F515F (TTC→TTT maeB ← malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
RA 2,583,790 G→A A579V (GCG→GTG)  aegA ← putative oxidoreductase AegA
RA 2,587,666 C→T T24T (ACC→ACT acrD → multidrug efflux pump RND permease AcrD
RA 2,637,689 G→A L322L (CTG→TTG)  bamB ← outer membrane protein assembly factor BamB
RA 2,652,867 G→A G125D (GGC→GAC)  sseA → 3‑mercaptopyruvate sulfurtransferase
RA 2,654,800 G→A A45V (GCT→GTT)  sseB ← protein SseB
RA 2,655,820 G→A P180L (CCA→CTA)  pepB ← aminopeptidase B
RA 2,662,928 G→A V132V (GTC→GTT trmJ ← tRNA Cm32/Um32 methyltransferase
RA 2,675,137 G→A P209S (CCG→TCG)  yphC ← putative zinc‑binding dehydrogenase YphC
RA 2,724,285 G→A R53Q (CGG→CAG)  yfiM → DUF2279 domain‑containing protein YfiM
RA 2,729,586 G→A intergenic (‑142/+30) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA
RA 2,779,623 C→T S1035N (AGC→AAC)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,783,462 C→T intergenic (‑736/+176) ypjA ← / ← ypjB adhesin‑like autotransporter YpjA/DUF5508 domain‑containing protein YpjB
RA 2,823,537 A→G L78P (CTG→CCG)  recA ← DNA recombination/repair protein RecA
RA 2,838,241 G→A intergenic (‑136/+13) hydN ← / ← ascG putative electron transport protein HydN/DNA‑binding transcriptional repressor AscG
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
RA 3,087,508 C→T P268L (CCA→CTA)  metK → methionine adenosyltransferase
RA 3,167,148 G→A H191Y (CAT→TAT)  ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
MC JC 3,360,851 Δ4,705 bp IS5‑mediated gltF[yhcE] gltF, yhcA, yhcD, [yhcE]
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,465,502 G→A E141K (GAG→AAG)  gspM → Type II secretion system protein GspM
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,542 G→A T141T (ACG→ACA crp → DNA‑binding transcriptional dual regulator CRP
RA 3,486,565 T→G L149R (CTG→CGG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,524,913 C→T F681F (TTC→TTT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,555,047 2 bp→TG coding (1963‑1964/2706 nt) malT → DNA‑binding transcriptional activator MalT
MC JC 3,559,895 Δ7 bp intergenic (+29/+721) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,685,480 C→T E47K (GAA→AAA)  pdeK ← c‑di‑GMP phosphodiesterase PdeK
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
JC JC 4,044,847 IS5 (+) +4 bp coding (649‑652/1431 nt) yihF → DUF945 domain‑containing protein YihF
JC JC 4,069,146 IS1 (–) +9 bp coding (123‑131/2037 nt) yihQ ← sulfoquinovosidase
JC JC 4,124,494 IS2 (+) +5 bp intergenic (‑38/+114) cytR ← / ← priA DNA‑binding transcriptional repressor CytR/primosomal protein N'
RA 4,166,554 C→T intergenic (+269/‑105) murI → / → rrsB glutamate racemase/16S ribosomal RNA
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,322,288 A→G F45F (TTT→TTC phnF ← putative transcriptional regulator PhnF
RA 4,325,000 C→T G56S (GGC→AGC)  phnC ← phosphonate/phosphate ABC transporter ATP binding subunit
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,549,043 T→G L79R (CTG→CGG)  fimH → type 1 fimbriae D‑mannose specific adhesin

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196292 1211454 15163 40 [39] [37] 40 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 3423799–3424233 3424451–3424238 6–653 40 [39] [39] 40 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 19785024 (0.040)139 (1.350) 84/582 0.0 98.6% intergenic (‑305/+16) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA5/uspC IS1 family protein InsA/universal stress protein C