breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 45 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091358,0650CA39.1% 159.7 / 32.5 110P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,054,8940GT34.0% 141.1 / 33.2 94P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009132,159,4130CT33.3% 49.2 / 13.5 36A343V (GCG→GTG) mdtCmultidrug efflux pump RND permease subunit MdtC
*NC_0009132,767,4450CA33.3% 245.1 / 29.0 129intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009132,722,8380GT32.4% 191.1 / 13.5 105E38* (GAG→TAG) pssAphosphatidylserine synthase
*NC_0009131,902,6130CA32.2% 167.3 / 14.3 90S189Y (TCC→TAC) manXmannose‑specific PTS enzyme IIAB component
*NC_0009134,296,1910AC32.1% 14.9 / 21.3 28intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009132,864,4260GT32.0% 184.1 / 10.9 125G64V (GGA→GTA) ygbMputative 2‑oxo‑tetronate isomerase YgbM
*NC_0009133,911,5330CA31.1% 273.3 / 24.0 161intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,767,4440CA31.0% 275.8 / 13.8 129intergenic (+89/‑266)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_00091312,1520GT29.9% 212.1 / 10.6 117intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009131,015,4880CA29.6% 234.3 / 16.8 135intergenic (+29/‑227)pqiC/rmfintermembrane transport lipoprotein PqiC/ribosome modulation factor
*NC_0009132,477,2650AC29.4% 176.5 / 12.7 85L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_000913878,3750GT29.4% 298.4 / 23.8 160R43L (CGA→CTA) bssRregulator of biofilm formation
*NC_0009131,922,8150CA28.3% 214.1 / 25.5 127G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009133,741,8710GT28.1% 223.8 / 14.5 121S221Y (TCC→TAC) plaRDNA‑binding transcriptional repressor PlaR
*NC_0009131,097,1180CA28.0% 76.8 / 20.2 50intergenic (+289/+447)ycdU/serXuncharacterized protein YcdU/tRNA‑Ser
*NC_0009132,073,6130GT27.9% 159.7 / 10.8 86R692L (CGG→CTG) fluself recognizing antigen 43 (Ag43) autotransporter
*NC_000913141,8490CA27.1% 235.9 / 19.4 118P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase
*NC_0009131,756,3530CA26.7% 248.7 / 14.0 120S219Y (TCC→TAC) pykFpyruvate kinase 1

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4542690166 (1.520)22 (0.210) 21/580 4.2 12.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 135 (1.280)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =162 (1.490)22 (0.210) 19/580 4.7 13.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 136 (1.290)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 439243781 (0.740)10 (0.120) 10/442 5.6 13.4% intergenic (+2/‑35) glyV/glyX tRNA‑Gly/tRNA‑Gly
?NC_000913 4392661 = 69 (0.860)intergenic (+3/‑231) glyY/yjeV tRNA‑Gly/uncharacterized protein YjeV
* ? NC_000913 = 3648146126 (1.160)8 (0.090) 8/482 6.9 7.4% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 98 (1.120)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 69674177 (0.710)6 (0.070) 6/442 7.3 8.6% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 70 (0.870)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 781577 =70 (0.640)10 (0.090)
+GAAG
9/590 7.7 11.1% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 93 (0.850)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 69650580 (0.730)4 (0.040) 4/538 9.6 5.7% intergenic (‑1/+37) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 696832 = 61 (0.620)intergenic (‑2/+33) glnW/glnU tRNA‑Gln/tRNA‑Gln
* ? NC_000913 = 1050545NA (NA)4 (0.040) 4/598 10.2 2.8% noncoding (768/768 nt) IS1 repeat region
?NC_000913 1902911 = 138 (1.270)coding (864/972 nt) manX mannose‑specific PTS enzyme IIAB component
* ? NC_000913 257908 =NA (NA)4 (0.040) 4/598 10.2 2.8% noncoding (768/768 nt) IS1 repeat region
?NC_000913 1902911 = 138 (1.270)coding (864/972 nt) manX mannose‑specific PTS enzyme IIAB component
* ? NC_000913 = 290416396 (0.880)6 (0.060) 3/540 10.3 5.5% intergenic (+789/+584) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
?NC_000913 2904408 = 120 (1.220)intergenic (+1034/+339) ygcE/queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE