breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 1,065,278 C→T R415C (CGT→TGT)  yccE → uncharacterized protein YccE
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,301,998 A→G S273G (AGC→GGC)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
MC JC 1,397,239 Δ7,315 bp IS5‑mediated ynaJ[abgA] ynaJ, uspE, fnr, ogt, abgT, abgB, [abgA]
MC JC 1,411,925 Δ23,060 bp [ttcA]ynaM 34 genes
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,737,584 A→G intergenic (‑39/‑260) cydH ← / → purR cytochrome bd‑I accessory subunit CydH/DNA‑binding transcriptional repressor PurR
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,867,453 C→A Q33Y (CAG→TAT rpoS ← RNA polymerase sigma factor RpoS
RA 2,867,455 G→A Q33Y (CAG→TAT)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,908,319 C→G D450H (GAC→CAC)  pyrG ← CTP synthetase
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
JC JC 3,890,892 IS1 (+) +9 bp coding (657‑665/1248 nt) tnaB → tryptophan:H(+) symporter TnaB
RA 3,980,226 G→A V91I (GTT→ATT)  rffM → UDP‑N‑acetyl‑D‑mannosaminuronic acid transferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,366,110 IS1 (+) +8 bp coding (657‑664/1302 nt) dcuA ← C4‑dicarboxylate transporter DcuA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978495 1979270 776 142 [0] [0] 170 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423807–3424231 3424486–3424238 8–680 39 [36] [38] 39 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =66 (0.610)50 (0.490) 40/566 1.6 40.9% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 82 (0.800)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780568 (0.620)53 (0.510) 46/566 1.1 42.0% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 82 (0.800)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994981 (0.010)133 (1.270) 85/574 0.1 99.2% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)133 (1.270) 81/574 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)141 (1.290) 83/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)169 (1.550) 102/598 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL