Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,147,792	0	T	A	79.6%	125.7 / 25.4	55	S58T (TCG→ACG)	plsX	putative phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (7/4);  new base A (25/18);  total (32/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACAT  >  NC_000913/1147549‑1148028                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             cTGCGTGATGAAGCTTAGGGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCt                                                                                                                                                                                                                                       >  2:327234/1‑251 (MQ=255)     gTGATGAAGCGTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCACACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGAGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCa                                                                                                                                                                                                                                    >  2:85587/1‑250 (MQ=255)       gatgaAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGc                                                                                                                                                                                                                                     >  2:45237/1‑247 (MQ=255)        atgaAGCTTATTGAGGATTTTCCCCAGGCAACGGGGGAAAGACCAAACCGGGCGGCGACGATTCCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCCCGCCATTCCTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGatt                                                                                                                                                                                                                                <  2:89456/251‑1 (MQ=255)              cTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGAGCCGATACCTTGACACGTCTAACCCTGGAGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCt                                                                                                                                                                                                                          >  1:31431/1‑251 (MQ=255)                  gTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGGGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTgcgc                                                                                                                                                                                                                      >  2:321647/1‑251 (MQ=255)                             ccccAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATc                                                                                                                                                                                                           >  1:95856/1‑251 (MQ=255)                               ccAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGc                                                                                                                                                                                                         >  1:227160/1‑251 (MQ=255)                               ccAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGc                                                                                                                                                                                                         <  1:7672/251‑1 (MQ=255)                                      aCTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAg                                                                                                                                                                                                      >  2:444581/1‑247 (MQ=255)                                                         gggCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCt                                                                                                                                                                                >  1:254156/1‑250 (MQ=255)                                                         gggCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTa                                                                                                                                                                               <  1:288730/251‑1 (MQ=255)                                                              gggacgaTACCTTGACACGTCTAACCCTGGCGTTAGATCTAATGGGAGGGGATTTGGGCCCTTACGGGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCACAACTCACTCTTCGGTTAGTCGGAAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACTTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGGGATGCCTGGCCTTCGTAAGCTATCcgc                                                                                                                                                                          <  2:37425/249‑1 (MQ=255)                                                                    aTACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGt                                                                                                                                                                    >  1:333943/1‑251 (MQ=255)                                                                       ccTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTGGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGt                                                                                                                                                                 <  2:256511/251‑1 (MQ=255)                                                                          tGACCCGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTgg                                                                                                                                                               <  1:265277/250‑1 (MQ=255)                                                                           gACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTggg                                                                                                                                                              >  1:404837/1‑250 (MQ=255)                                                                                       ccTGGAGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGcgcg                                                                                                                                                 >  2:105094/1‑251 (MQ=255)                                                                                           gCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGc                                                                                                                                             <  2:207484/251‑1 (MQ=255)                                                                                            cGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGcc                                                                                                                                            >  1:111938/1‑251 (MQ=255)                                                                                                aGATGTCATGGGAGGGGGTTTTGGACCTTCCGTGACAGTGCCTGCAGCATTACAAGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCAGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGACAGTTATCGCCAGGGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGGTCAATGCGCGTGGCCCTgg                                                                                                                                        >  1:126040/1‑251 (MQ=255)                                                                                                 gATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTgg                                                                                                                                        >  1:119088/1‑250 (MQ=255)                                                                                                        tGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTg                                                                                                                                >  1:382189/1‑251 (MQ=255)                                                                                                                        cccNTACGTGACAGGGCCTCCCGCATTTCAGGCCCTGAATTCCAATTCGGACCTCACTCTCCTTCTAGTCGTCAATTTCTCCGCCATCGCGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCGAGTGATGCCCGGCCCTCGCAAGCTATCCTCCCCAGTCGTGGTAGTTCAATGCGCGTGGCCTTCGTGCTGCTGAACGAAGGGCGAgccc                                                                                                                <  2:450779/251‑3 (MQ=255)                                                                                                                             ccGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGc                                                                                                            <  1:268311/250‑1 (MQ=255)                                                                                                                                    aGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTc                                                                                                     >  2:360312/1‑250 (MQ=255)                                                                                                                                     gTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTc                                                                                                     >  2:26598/1‑249 (MQ=255)                                                                                                                                      tGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGACAGAAGGTCGAGCGCAAGCCTGTGTCAGt                                                                                                  >  2:320050/1‑251 (MQ=255)                                                                                                                                        ccTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCAGCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGc                                                                                                <  2:417850/251‑1 (MQ=255)                                                                                                                                           gcagcaTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCgg                                                                                             >  2:176054/1‑251 (MQ=255)                                                                                                                                           gcagcaTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCg                                                                                              <  1:169979/250‑1 (MQ=255)                                                                                                                                               caTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCATTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAAt                                                                                         <  1:468740/251‑1 (MQ=255)                                                                                                                                                aTTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAAt                                                                                         >  1:416027/1‑250 (MQ=255)                                                                                                                                                        cACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGgcg                                                                                >  2:407587/1‑251 (MQ=255)                                                                                                                                                             aaTTCTAATTCGCAACTCACTCTTCTTTTAGTCGGAAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGAt                                                                           <  2:111938/251‑1 (MQ=255)                                                                                                                                                                  tAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGc                                                                      >  2:137486/1‑251 (MQ=255)                                                                                                                                                                                     cttTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCa                                                    >  1:148326/1‑250 (MQ=255)                                                                                                                                                                                       tttAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCa                                                    <  2:119088/248‑1 (MQ=255)                                                                                                                                                                                       tttAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAg                                                  <  1:360312/250‑1 (MQ=255)                                                                                                                                                                                           gTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCGGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGGGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCt                                             <  2:191663/251‑1 (MQ=255)                                                                                                                                                                                           ccgtagttcttccgatctaTCACGCCATTCCTTGCTAAATCTGACTTTGAATAACGTCCGCGTCGGCAGATTTTTCCTTCGCAGTCAGTTATCGCCAGTGATGCCCGTCCTTCGCAAGCTTTCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGAGTTGATGGGGCTGGCAAAATTATTACTCAAGCCCCt                                             <  2:372492/233‑1 (MQ=255)                                                                                                                                                                                                 aaTTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCaa                                                                                                                                                       >  1:276420/1‑139 (MQ=255)                                                                                                                                                                                                     ccGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGACGAGCGCAAGCCTGTGTCAGAGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGAtt                                   >  2:216197/1‑251 (MQ=255)                                                                                                                                                                                                         cGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGc                               <  2:194683/251‑1 (MQ=255)                                                                                                                                                                                                           ccATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGt                             <  1:407587/251‑1 (MQ=255)                                                                                                                                                                                                             aTCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCt                                             >  1:372492/1‑233 (MQ=255)                                                                                                                                                                                                               cACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                          <  1:26598/250‑1 (MQ=255)                                                                                                                                                                                                               cACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCg                          <  2:28167/250‑1 (MQ=255)                                                                                                                                                                                                                aCGCCATTACTTGCTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgc                        >  2:465754/1‑251 (MQ=255)                                                                                                                                                                                                                  gCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGgcgc                      >  2:178156/1‑251 (MQ=255)                                                                                                                                                                                                                             cTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATAc                                                                                       <  1:4327/175‑1 (MQ=255)                                                                                                                                                                                                                             cTAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATAc                                                                                       >  2:4327/1‑175 (MQ=255)                                                                                                                                                                                                                              tAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTg                 <  1:97923/244‑1 (MQ=255)                                                                                                                                                                                                                              tAAAGCTGACTTTGAACAACGTACGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTg                 >  2:97923/1‑244 (MQ=255)                                                                                                                                                                                                                                      aCTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGTGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACAt  >  2:417460/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                             CTGCGTGATGAAGCTTAGTGAGGATTTTCCCCAGGCAACTGGGGAAAGACCAAACCGGGCGGCGACGATACCTTGACACGTCTAACCCTGGCGTTAGATGTCATGGGAGGGGATTTTGGCCCTTCCGTGACAGTGCCTGCAGCATTGCAGGCACTGAATTCTAATTCGCAACTCACTCTTCTTTTAGTCGGCAATTCCGACGCCATCACGCCATTACTTGCTAAAGCTGACTTTGAACAACGTTCGCGTCTGCAGATTATTCCTGCGCAGTCAGTTATCGCCAGTGATGCCCGGCCTTCGCAAGCTATCCGCGCCAGTCGTGGGAGTTCAATGCGCGTGGCCCTGGAGCTGGTGAAAGAAGGTCGAGCGCAAGCCTGTGTCAGTGCCGGTAATACCGGGGCGCTGATGGGGCTGGCAAAATTATTACTCAAGCCCCTGGAGGGGATTGAGCGTCCGGCGCTGGTGACGGTATTACCACAT  >  NC_000913/1147549‑1148028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑