breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 108,585 C→T R103C (CGT→TGT)  secA → protein translocation ATPase
RA 134,256 G→T intergenic (+44/‑132) acnB → / → yacL AcnB/PF06062 family protein YacL
RA 156,068 A→G V45A (GTA→GCA)  yadV ← putative fimbrial chaperone YadV
RA 157,043 G→A intergenic (‑160/+210) yadN ← / ← folK putative fimbrial protein YadN/2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
JC JC 167,915 IS2 (–) +5 bp coding (432‑436/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 287,356 C→T L190L (CTG→TTG)  yagH → putative xylosidase/arabinosidase
RA 289,091 A→G L24L (CTT→CTC xynR ← DNA‑binding transcriptional repressor XynR
RA 306,212 2 bp→AA coding (605‑606/1644 nt) ecpD ← fimbrial adhesin EcpD
RA 322,064 C→T L153L (CTG→TTG)  ykgE → putative lactate utilization oxidoreductase YkgE
JC JC 362,204 IS1 (–) +9 bp coding (968‑976/1254 nt) lacY ← lactose permease
JC JC 432,120 IS1 (+) +9 bp intergenic (‑107/+184) tsx ← / ← yajI nucleoside‑specific channel‑forming protein Tsx/putative lipoprotein YajI
RA 458,188 G→A D255N (GAT→AAT)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 459,432 C→T S182F (TCT→TTT)  lon → Lon protease
RA 462,394 C→A L160L (CTC→CTA ppiD → periplasmic folding chaperone
RA 489,655 C→G V1040V (GTC→GTG mscK → potassium dependent, small conductance mechanosensitive channel
RA 543,263 2 bp→GT coding (783‑784/786 nt) allE ← (S)‑ureidoglycine aminohydrolase
MC JC 575,786 Δ3,398 bp IS5‑mediated [nmpC]borD [nmpC], essD, rrrD, rzpD, rzoD, borD
RA 628,569 A→G N7D (AAT→GAT)  entA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
RA 659,517 G→A L234L (CTG→TTG)  lipA ← lipoyl synthase
RA 659,527 G→T T230T (ACC→ACA lipA ← lipoyl synthase
RA 696,470 C→T noncoding (35/75 nt) glnX ← tRNA‑Gln
RA 727,761 C→T E324E (GAG→GAA kdpA ← K(+) transporting P‑type ATPase subunit KdpA
RA 758,593 T→C intergenic (+188/‑113) sdhB → / → sucA succinate:quinone oxidoreductase, iron‑sulfur cluster binding protein/subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 768,617 G→A E214K (GAG→AAG)  mngB → alpha‑mannosidase
RA 788,983 C→A G333C (GGC→TGC)  galK ← galactokinase
RA 789,853 C→A V43F (GTT→TTT)  galK ← galactokinase
RA 790,086 C→T G315D (GGT→GAT)  galT ← galactose‑1‑phosphate uridylyltransferase
RA 856,434 C→T P158S (CCT→TCT)  ybiT → putative ATP‑binding protein YbiT
RA 857,041 C→A T360K (ACG→AAG)  ybiT → putative ATP‑binding protein YbiT
RA 873,398 Δ1 bp coding (420/2349 nt) pdeI → putative c‑di‑GMP phosphodiesterase PdeI
MC JC 923,998 Δ3 bp coding (735‑737/2277 nt) clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
RA 973,577 C→T S14F (TCC→TTC)  cmoM → tRNA cmo(5)U34 methyltransferase
RA 1,005,691 T→A I308I (ATT→ATA pyrD → dihydroorotate dehydrogenase, type 2
RA 1,005,703 C→T A312A (GCC→GCT pyrD → dihydroorotate dehydrogenase, type 2
RA 1,043,607 G→A L202L (CTG→TTG)  etk ← protein‑tyrosine kinase Etk
RA 1,050,092 C→T S87L (TCA→TTA) 
L22L (CTC→CTT
insA4 →
insB4 →
IS1 family protein InsA
IS1 family protein InsB
RA 1,146,079 C→A V173F (GTT→TTT)  yceF ← m(7)GTP pyrophosphatase
RA 1,157,167 A→G T131A (ACC→GCC)  ycfH → putative metal‑dependent hydrolase YcfH
RA 1,157,902 G→T V12F (GTC→TTC)  ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,195,620 A→G K166K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase
RA 1,220,493 C→T intergenic (+1292/+1812) ymgF → / ← ymgD inner membrane protein YmgF/PF16456 family protein YmgD
RA 1,233,669 G→A S350F (TCT→TTT)  nhaB ← Na(+):H(+) antiporter NhaB
RA 1,290,947 C→T Q236* (CAA→TAA)  rssB → regulator of RpoS
RA 1,292,992 A→C intergenic (‑70/‑535) hns ← / → tdk DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,318,275 Δ1 bp coding (141/1194 nt) trpB ← tryptophan synthase subunit beta
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
MC JC 1,369,587 Δ26,457 bp IS5‑mediated [pspD]ynaI 25 genes
RA 1,405,758 G→A intergenic (‑7/‑221) mcaS ← / → smrA small regulatory RNA McaS/DNA endonuclease SmrA
RA 1,425,329 Δ1 bp intergenic (+90/‑48) trkG → / → ynaK K(+) transporter TrkG/ParB‑like nuclease domain‑containing protein YnaK
RA 1,433,144 G→A F177F (TTC→TTT pinR ← putative site‑specific recombinase
RA 1,531,689 A→G intergenic (‑1185/‑127) yncO ← / → ydcC protein YncO/H repeat‑associated putative transposase YdcC
RA 1,553,630 C→A G70V (GGT→GTT)  adhP ← ethanol dehydrogenase/alcohol dehydrogenase
RA 1,555,731 Δ1 bp intergenic (‑62/+95) maeA ← / ← sra malate dehydrogenase (oxaloacetate‑decarboxylating)/ribosome‑associated protein Sra
JC JC 1,596,039 IS2 (+) +5 bp intergenic (‑3597/+2574) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,600,234 C→T intergenic (‑25/+54) lsrK ← / ← lsrR autoinducer‑2 kinase/DNA‑binding transcriptional repressor LsrR
RA 1,601,098 C→A S48S (TCG→TCT lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,601,103 C→T V47M (GTG→ATG)  lsrR ← DNA‑binding transcriptional repressor LsrR
RA 1,632,712 A→G intergenic (‑427/‑178) ydfJ ← / → ynfT putative transporter YdfJ/protein YnfT
RA 1,641,002 T→C intergenic (+27/+337) ynfU → / ← cspB putative zinc‑binding protein YnfU/cold shock‑like protein CspB
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,649,971 C→T P60S (CCG→TCG)  ydfD → lysis protein
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,702,757 C→T F175F (TTC→TTT add → adenosine deaminase
RA 1,737,913 A→T K24* (AAA→TAA)  purR → DNA‑binding transcriptional repressor PurR
RA 1,793,377 Δ1 bp coding (182/750 nt) btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,861,311 C→T G8S (GGC→AGC)  ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,874,202 G→A intergenic (‑20/‑150) yoaI ← / → yeaL protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
RA 1,882,591 G→A C227Y (TGC→TAC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
JC JC 1,907,439 IS1 (+) +8 bp intergenic (‑4/+2) cspC ← / ← yobF transcription antiterminator and regulator of mRNA stability CspC/DUF2527 domain‑containing protein YobF
RA 1,911,929 2 bp→TT coding (647‑648/882 nt) htpX ← protease HtpX
RA 1,915,132 G→T P135T (CCG→ACG)  proQ ← RNA chaperone ProQ
RA 1,915,945 C→T G62S (GGC→AGC)  msrC ← free methionine‑(R)‑sulfoxide reductase
RA 1,936,634 C→T intergenic (‑320/‑18) zwf ← / → yebK NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase/DNA‑binding transcriptional repressor YebK
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
MC JC 2,092,996 (TGGC)4→3 coding (599‑602/1071 nt) hisC → histidinol‑phosphate aminotransferase
RA 2,099,525 A→T I30N (ATT→AAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
JC JC 2,150,948 IS1 (+) +9 bp coding (30‑38/1947 nt) yegI ← putative serine/threonine‑protein kinase YegI
RA 2,155,880 C→T L206L (CTC→CTT mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,224,169 A→G F234F (TTT→TTC pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,237,105 G→T P217T (CCG→ACG)  mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
JC JC 2,237,684 IS5 (+) +4 bp coding (67‑70/1011 nt) mglC ← D‑galactose/methyl‑galactoside ABC transporter membrane subunit
RA 2,264,460 2 bp→AT coding (598‑599/1182 nt) setB → sugar exporter SetB
RA 2,319,078 C→A D267Y (GAT→TAT)  rcsC ← histidine kinase RcsC
RA 2,360,550 Δ1 bp coding (865/1206 nt) rhmD ← L‑rhamnonate dehydratase
RA 2,392,233 C→G G270A (GGT→GCT)  nuoM ← NADH:quinone oxidoreductase subunit M
RA 2,423,891 C→T V207I (GTA→ATA)  hisP ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, ATP binding subunit
RA 2,443,447 G→A A562T (GCG→ACG)  mnmC → fused tRNA 5‑aminomethyl‑2‑thiouridylate methyltransferase and tRNA 5‑aminomethyl‑2‑thiouridylate synthase
JC JC 2,448,805 IS2 (–) +5 bp coding (200‑204/552 nt) smrB → putative endonuclease SmrB
RA 2,473,392 G→A intergenic (‑146/‑212) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,473,397 +T intergenic (‑151/‑207) yfdO ← / → yfdP protein YdfO/uncharacterized protein YfdP
RA 2,510,609 C→T P327L (CCG→CTG)  yfeO → putative transport protein YfeO
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,580,956 Δ1 bp coding (1321/2004 nt) tktB → transketolase 2
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,597,615 G→A Q2* (CAA→TAA)  purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthase
RA 2,630,852 G→A S176N (AGC→AAC)  yfgI → nalidixic acid resistance protein YfgI
RA 2,633,322 Δ1 bp coding (749/1467 nt) guaB ← inosine 5'‑monophosphate dehydrogenase
RA 2,732,992 G→A I394I (ATC→ATT
I394I (ATC→ATT
clpB ←
clpB ←
chaperone protein ClpB
chaperone protein ClpB
RA 2,739,269 Δ1 bp coding (801/1122 nt) tyrA ← fused chorismate mutase/prephenate dehydrogenase
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
RA 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,981,924 C→T G87R (GGG→AGG)  araE ← arabinose:H(+) symporter
RA 2,982,000 C→T Q61Q (CAG→CAA araE ← arabinose:H(+) symporter
RA 3,040,842 2 bp→AA coding (228‑229/267 nt) sdhE ← FAD assembly factor
RA 3,043,360 G→A I88I (ATC→ATT yqfB ← N(4)‑acetylcytidine amidohydrolase
RA 3,071,412 C→T intergenic (‑168/+47) fbaA ← / ← pgk fructose‑bisphosphate aldolase class II/phosphoglycerate kinase
RA 3,089,109 A→T K276* (AAA→TAA)  galP → galactose:H(+) symporter
RA 3,132,532 G→A P227P (CCC→CCT yghR ← putative ATP‑binding protein YghR
RA 3,161,616 G→A R352C (CGC→TGC)  ftsP ← cell division protein FtsP
RA 3,167,566 C→T V51V (GTG→GTA ygiS ← putative deoxycholate binding periplasmic protein
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,242,343 C→T M324I (ATG→ATA uxaA ← D‑altronate dehydratase
JC JC 3,262,484 IS5 (+) +4 bp coding (1174‑1177/1209 nt) tdcD ← propionate kinase
JC JC 3,291,225 IS2 (+) +5 bp coding (2412‑2416/2517 nt) yraJ → putative fimbrial usher protein YraJ
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
MC JC 3,349,206 Δ16,350 bp IS5‑mediated [mtgA][yhcE] [mtgA], elbB, arcZ, arcB, yhcC, gltB, gltD, gltF, yhcA, yhcD, [yhcE]
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,423,776 G→T noncoding (12/120 nt) rrfD ← 5S ribosomal RNA
RA 3,432,105 C→T L120L (CTG→CTA smg ← DUF494 domain‑containing protein Smg
RA 3,443,961 G→A A46V (GCC→GTC)  secY ← Sec translocon subunit SecY
RA 3,443,980 G→A P40S (CCG→TCG)  secY ← Sec translocon subunit SecY
RA 3,452,022 G→A P89S (CCG→TCG)  rplD ← 50S ribosomal subunit protein L4
RA 3,452,218 G→A F23F (TTC→TTT rplD ← 50S ribosomal subunit protein L4
RA 3,457,378 C→T L334L (CTC→CTT gspD → Type II secretion system protein GspD
RA 3,458,245 A→C S623S (TCA→TCC gspD → Type II secretion system protein GspD
RA 3,474,425 T→G K43T (AAA→ACA)  rpsL ← 30S ribosomal subunit protein S12
RA 3,494,679 C→T T223T (ACC→ACT nirB → nitrite reductase (NADH) large subunit
RA 3,525,566 T→C intergenic (+143/+23) mrcA → / ← nudE peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA/ADP‑sugar diphosphatase NudE
RA 3,537,999 C→T F205F (TTC→TTT yhgF → putative RNA‑binding protein YhgF
RA 3,555,047 G→T V655F (GTC→TTC)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,606,891 C→T F147F (TTC→TTT zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,617,379 T→A I122K (ATA→AAA)  nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,624,545 T→A D56E (GAT→GAA yhhI → putative transposase
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,679,508 C→T T30T (ACC→ACT kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,686,705 G→A F823F (TTC→TTT bcsC ← cellulose synthase outer membrane channel
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,712,407 G→A G176G (GGC→GGT yhjY ← putative outer membrane protein YhjY
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,955 2 bp→AA coding (810‑811/1323 nt) xylA ← xylose isomerase
JC JC 3,731,420 IS1 (–) +9 bp coding (290‑298/993 nt) xylF → xylose ABC transporter periplasmic binding protein
RA 3,769,447 G→A E35K (GAA→AAA)  yibV → PF15596 family protein YibV
RA 3,773,040 (G)6→4 coding (760‑761/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,783,983 G→A S49F (TCC→TTC)  secB ← protein export chaperone SecB
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,865,885 A→G V130A (GTT→GCT)  yidE ← putative transport protein YidE
RA 3,899,195 C→T E67K (GAA→AAA)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,903,234 G→A V156V (GTC→GTT bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,961,662 G→T G329V (GGC→GTC)  rep → ATP‑dependent DNA helicase Rep
RA 3,965,174 G→A H153Y (CAC→TAC)  rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,968,683 G→A M256I (ATG→ATA rfe → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 3,969,748 T→C S240P (TCG→CCG)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,999,664 C→T S561L (TCG→TTG)  uvrD → DNA helicase II
RA 4,009,698 G→A A177T (GCA→ACA)  pldB → lysophospholipase L2
RA 4,017,197 C→T intergenic (+5/‑136) udp → / → rmuC uridine phosphorylase/putative recombination limiting protein RmuC
RA 4,084,903 T→G N307T (AAC→ACC)  fdoG ← formate dehydrogenase O subunit alpha
RA 4,092,703 A→G F41L (TTC→CTC)  frvA ← putative PTS enzyme IIA component FrvA
RA 4,113,851 G→A H208Y (CAT→TAT)  fpr ← flavodoxin/ferredoxin‑NADP(+) reductase
RA 4,141,577 A→G I215I (ATT→ATC ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,143,377 C→T S283L (TCA→TTA)  frwC → putative PTS enzyme IIC component FrwC
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,215,347 T→A intergenic (+138/‑131) metA → / → aceB homoserine O‑succinyltransferase/malate synthase A
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,293,781 2 bp→AT coding (239‑240/597 nt) nrfG → putative formate‑dependent nitrite reductase complex subunit NrfG
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,400,254 C→T intergenic (+52/‑34) miaA → / → hfq tRNA dimethylallyltransferase/RNA‑binding protein Hfq
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
JC JC 4,542,042 IS1 (+) +8 bp coding (6‑13/597 nt) fimE → regulator for fimA
RA 4,545,746 T→C I217I (ATT→ATC fimD → type I fimbriae usher protein
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196314 1211461 15148 32 [29] [29] 32 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1632735–1632847 1633412–1632935 89–678 32 [31] [31] 32 ynfT–ydfK ynfT,ydfK
* * ÷ NC_000913 3423810–3424234 3426666–3424239 6–2857 32 [30] [31] 34 [rrlD] [rrlD]
* * ÷ NC_000913 3762413–3764074 3765136–3764079 6–2724 32 [30] [31] 32 rhsA rhs element protein RhsA
* * ÷ NC_000913 4035418–4035730 4036916–4035736 7–1499 32 [31] [30] 33 [rrsA] [rrsA]
* * ÷ NC_000913 4038058–4038686 4039210–4038691 6–1153 32 [31] [31] 33 rrlA 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4254774 (0.750)39 (0.390) 31/598 2.1 34.5% coding (145/771 nt) fixA putative electron transfer flavoprotein FixA
?NC_000913 273955 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 12994980 (0.000)138 (1.420) 81/590 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)41 (0.420) 33/596 1.9 34.4% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 1667532 = 78 (0.790)coding (1033/1221 nt) mlc DNA‑binding transcriptional repressor Mlc
* ? NC_000913 = 141062592 (0.930)122 (1.240) 80/598 0.1 57.0% coding (1115/1374 nt) dbpA ATP‑dependent RNA helicase DbpA
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1469296 =NA (NA)34 (0.350) 24/596 3.0 33.0% noncoding (1/1221 nt) IS30 repeat region
?NC_000913 4509606 = 69 (0.700)noncoding (600/795 nt) IS911 repeat region
* ? NC_000913 = 166753577 (0.780)39 (0.390) 34/598 1.8 33.6% coding (1030/1221 nt) mlc DNA‑binding transcriptional repressor Mlc
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 1979270NA (NA)31 (0.320) 24/594 3.0 28.4% noncoding (1/768 nt) IS1 repeat region
?NC_000913 2619885 = 78 (0.800)coding (276/1290 nt) uraA uracil:H(+) symporter UraA
* ? NC_000913 2288919 =NA (NA)37 (0.370) 31/598 2.1 26.6% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 4540910 = 102 (1.030)intergenic (‑1409/‑47) nanC/fimB N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
* ? NC_000913 = 2290113NA (NA)43 (0.440) 39/598 1.4 29.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 4540913 105 (1.060)intergenic (‑1412/‑44) nanC/fimB N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
* ? NC_000913 = 3653230NA (NA)44 (0.450) 36/598 1.6 30.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3793847 100 (1.010)intergenic (‑164/‑140) htrL/rfaD protein HtrL/ADP‑L‑glycero‑D‑mannoheptose 6‑epimerase
* ? NC_000913 4542682 =45 (0.460)116 (1.210) 72/580 0.1 74.7% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 35 (0.370)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269047 (0.480)115 (1.200) 81/580 0.0 74.1% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 35 (0.370)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit