Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	788,983	C→A	G333C (GGC→TGC)	galK ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	788,983	0	C	A	100.0%	220.3 / NA	61	G333C (GGC→TGC)	galK	galactokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/33);  total (28/33)

GACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACG  >  NC_000913/788691‑789277                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       gACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGTTTGCTGTACGGCAGGCACCAGCTCTTACGGGATCAGCGCGACGATACAGCCGCCACATCCGCCGCAGGTCATg                                                                                                                                                                                                                                                                                                 >  2:237898/1‑300 (MQ=255)   cGCTACCATCGGAGAGCGAAATACGGGCGGGAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACGACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTGCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCg                                                                                                                                                                                                                                                                                               <  2:213321/300‑1 (MQ=255)         cATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGcc                                                                                                                                                                                                                                                                                         <  1:748266/300‑1 (MQ=255)                                                  cAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCagag                                                                                                                                                                                                                                                <  2:266806/300‑1 (MQ=255)                                                  cAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCagag                                                                                                                                                                                                                                                >  1:315430/1‑300 (MQ=255)                                                      ccATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTTACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCNCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTc                                                                                                                                                                                                                                            >  1:544436/1‑300 (MQ=255)                                                           aGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCCTATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTACGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGTGTCCGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAAttt                                                                                                                                                                                                                                       >  2:13721/1‑300 (MQ=255)                                                            gCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCANCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTANGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTg                                                                                                                                                                                                                                      >  2:724106/1‑300 (MQ=255)                                                              gTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCg                                                                                                                                                                                                                                    >  2:317973/1‑300 (MQ=255)                                                                  cTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTACGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCAc                                                                                                                                                                                                                                >  2:241448/1‑300 (MQ=255)                                                                        tccatGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGAt                                                                                                                                                                                                                          >  1:745632/5‑300 (MQ=255)                                                                        tCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTAACAGAGTGTCAATTTGCGGCACGGTGAt                                                                                                                                                                                                                          >  1:745235/1‑300 (MQ=255)                                                                                  gttACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTATTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAatca                                                                                                                                                                                                                >  2:97741/1‑300 (MQ=255)                                                                                               aaCAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTNGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAgg                                                                                                                                                                                                   <  1:545501/300‑1 (MQ=255)                                                                                                     cGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATgag                                                                                                                                                                                             >  1:251084/1‑300 (MQ=255)                                                                                                            gcggACCACCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCGCCTTTTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAACTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGc                                                                                                                                                                                      <  2:369846/297‑1 (MQ=255)                                                                                                                             aGTGTGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATTGTTTACAAACGTAAAAAGTCCCTTTAATACCTGTTTTTGCTTCATATTTTTCATCGACAGCTTGCTGTACGGCATGCACCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCGAATCCGCCGCAGTTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTGTGACGATTGCTACCAGAGGGGCAATGTGCGGCACGGTGATGGCGAAACAATCGCGCATATAGGTATgagatgcagacatcgaaggggataggc                                                                                                                                                                     <  1:630388/300‑24 (MQ=255)                                                                                                                              gTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACg                                                                                                                                                                    >  1:242837/1‑300 (MQ=255)                                                                                                                               ggCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTTTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTGTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACTGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGt                                                                                                                                                                   <  2:405255/299‑1 (MQ=255)                                                                                                                                       tttCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCTTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTc                                                                                                                                                           <  1:203937/300‑1 (MQ=255)                                                                                                                                       tttCGGTCAGCACTGTCCTGCTCCTTTTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGTTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGTACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTc                                                                                                                                                           <  1:203812/300‑1 (MQ=255)                                                                                                                                           gTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCt                                                                                                                                                       >  1:179488/1‑300 (MQ=255)                                                                                                                                                gCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGTTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGACAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGAGAATAGAGGCATGAGAATCCGCCATCAACTCGCCCATACGTTTCCGGTCGACTTGCTc                                                                                                                                                  >  2:396016/1‑300 (MQ=255)                                                                                                                                                     ttCCTGCTCCTTGTGATGGTTTACAAACGTAAAAATTCTCTTTAATACCTGTGTTTGCTTCATATTGTTCATCTACAGCTTGCTGTACGACAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGTCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTTTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGTTGATTTCGAAATCATCGCGCATAGAGGCATTAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcg                                                                                                                                             <  2:275611/299‑1 (MQ=255)                                                                                                                                                     gTCCTGCTCCTTGTGATGGTTTCCAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGCCAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGACAATCACAGCTTTGACGATTTATACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCCGGTCGCCTTGCTCCAgcg                                                                                                                                             >  1:573307/1‑300 (MQ=255)                                                                                                                                                     gTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATTAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCAACTTTGTCGCCAATCACAGCTTTGCCGATTTCTACCAGAGTGTCAATTTGTGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcg                                                                                                                                             >  2:34709/1‑300 (MQ=255)                                                                                                                                                     gTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcg                                                                                                                                             >  2:43901/1‑300 (MQ=255)                                                                                                                                                     gTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcg                                                                                                                                             >  1:212537/1‑300 (MQ=255)                                                                                                                                                     gTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCAGACAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAgcg                                                                                                                                             >  2:218166/1‑300 (MQ=255)                                                                                                                                                                       ggttACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTAATATTGTTCAGCGACAGCTTGCTGTACGACAGGCACCAGCTCTCACAGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTATCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGTGGCACGGCGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGACATCAACTCGCACATACGTTTCAGGTCGCCTTGCTCCAGCGCGATGGCAGCTTCAACgg                                                                                                                           >  2:766777/3‑300 (MQ=255)                                                                                                                                                                       gTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACgg                                                                                                                           <  1:31179/300‑1 (MQ=255)                                                                                                                                                                               aCGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCg                                                                                                                   <  1:374138/300‑1 (MQ=255)                                                                                                                                                                                              ttAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCNCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGtata                                                                                                    <  2:580523/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACTGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCAGAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  1:414166/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  1:222038/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  2:741418/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGTTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  2:698218/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  2:281904/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  1:10318/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGTTTGTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCGAATCCGCCGCAGTTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGGCGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCGGTATGTGac                                                                                                <  2:267121/300‑1 (MQ=255)                                                                                                                                                                                                  tACCTGGTTTTGCTTCATATTGTGCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGac                                                                                                <  1:735335/300‑1 (MQ=255)                                                                                                                                                                                                                           tataagagacagCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTc                                                                       <  1:313042/289‑1 (MQ=255)                                                                                                                                                                                                                                       cTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTcctgtctcttata                                                           >  2:311176/1‑289 (MQ=255)                                                                                                                                                                                                                                         gTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCaa                                                         >  1:354438/1‑300 (MQ=255)                                                                                                                                                                                                                                         gTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCACCAATCACAGCTTTGACGATTTCTAACAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATGGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGCCAGTATATGAAGCACGCGTTTTGCCAAGATCGGGTCCAGTTCATGCGCaa                                                         >  2:7517/1‑300 (MQ=255)                                                                                                                                                                                                                                          tACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAAc                                                        <  1:319906/300‑1 (MQ=255)                                                                                                                                                                                                                                               caggcaCCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGt                                                   <  2:277660/300‑1 (MQ=255)                                                                                                                                                                                                                                               caggcaCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGt                                                   <  1:242859/300‑1 (MQ=255)                                                                                                                                                                                                                                                   caCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCACCAGCGTTGaa                                               >  1:15152/1‑300 (MQ=255)                                                                                                                                                                                                                                                   caCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGaa                                               >  1:131302/1‑300 (MQ=255)                                                                                                                                                                                                                                                    aCCAGCTCTTCCTGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGNGGCATGAGACTCCGCCATCAACTCGCNCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAAc                                              <  2:520742/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGTTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAAc                                              <  2:353487/300‑1 (MQ=255)                                                                                                                                                                                                                                                    aCCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAAc                                              <  2:226736/300‑1 (MQ=255)                                                                                                                                                                                                                                                           ctTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGNACATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCaa                                       >  2:190256/1‑300 (MQ=255)                                                                                                                                                                                                                                                           ctTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGCTCGGGTCCAGTTCATGCGCAACAACGTTGAACTCTTCaa                                       >  2:343714/1‑300 (MQ=255)                                                                                                                                                                                                                                                                    tCAGCGCGACGATACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACAt                              <  1:607977/300‑1 (MQ=255)                                                                                                                                                                                                                                                                           gacgaTACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAg                       <  1:745684/300‑1 (MQ=255)                                                                                                                                                                                                                                                                              gaTACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAANCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGggc                    <  1:530165/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               aTACAGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATCTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGggct                   >  1:776782/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                   aGCCGCCAAATCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGggctggct               <  1:98010/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                          aaaTCCGCCGCAGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGgaa        <  1:43989/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                gccgcagGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATCTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAAcg  >  1:740371/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                       GACGCTGCCATCGGAAAGCGGAATACGGGCGGAAAGTAAAGTCGCACCCCAGTCCATCAGCGTGACTACCATCCCTGCGTTGTTACGCAAAGTTAACAGTCGGTACGGCTGACCATCGGGTGCCAGTGCGGGAGTTTCGTTCAGCACTGTCCTGCTCCTTGTGATGGTTTACAAACGTAAAAAGTCTCTTTAATACCTGTTTTTGCTTCATATTGTTCAGCGACAGCTTGCTGTACGGCAGGCACCAGCTCTTCCGGGATCAGCGCGACGATACAGCCGCCAAATCCGCCGCCGGTCATGCGTACGCCACCTTTGTCGCCAATCACAGCTTTGACGATTTCTACCAGAGTGTCAATTTGCGGCACGGTGATTTCGAAATCATCGCGCATAGAGGCATGAGACTCCGCCATCAACTCGCCCATACGTTTCAGGTCGCCTTGCTCCAGCGCGCTGGCAGCTTCAACGGTGCGGGCGTTTTCAGTCAGTATATGACGCACGCGTTTTGCCACGATCGGGTCCAGTTCATGCGCAACAGCGTTGAACTCTTCAATGGTGACATCACGCAGGGCTGGCTGCTGGAAGAAACG  >  NC_000913/788691‑789277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑