Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,423,776	G→T	noncoding (12/120 nt)	rrfD ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,423,776	0	G	T	100.0%	121.2 / NA	37	noncoding (12/120 nt)	rrfD	5S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/7);  total (30/7)

GCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CA‑TCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTAC  >  NC_000913/3423481‑3423936                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                   gCGTTTCCCTTCTGAGTTAGGCATGGGTTCGGGTGGGACTACCGCTCTACTGCCGCCAGACAGATTCTTTTCTAATAGGCTGAACTTTAACTTAAAGATGGGTGTTGATACCCGGAGTCGAATTGGGGACCTAACCCTGACCAAAGGTGCGCTCTACCAACTAAGCCATAGCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccg                                                                                                                                                                <  1:497699/300‑1 (MQ=18) gCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTACTCTGCCGAACCTTAACCTAAAACGTGGTGCTGATACCCAGAGTCGAACCGAGAACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGCTGCCTGGCAGTTCCCTACTCTTGCATGGGGAGACCCCACACTACCATCGGCTCTACGGCGTTTCAATTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTgccg                                                                                                                                                                >  1:291724/1‑300 (MQ=37)                          gggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAACATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGAc                                                                                                                                       >  1:174906/1‑300 (MQ=255)                          gggTCAGGTGGGACCACCGCGCTACTGCCGACAGACAAATTCTTTTCTAATCTGCCGAGCTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCATACTCTCGCATGGGGAGACCCCACACTACCGTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCCTGGGGTCAGATGGGCCCAACGCGCTACTACCGCCCGGCAAATTCCGTTTTATCAGAc                                                                                                                                       >  2:63796/1‑300 (MQ=37)                           ggTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGAcc                                                                                                                                      >  1:585946/1‑300 (MQ=255)                              cAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCt                                                                                                                                   >  1:591785/1‑300 (MQ=255)                              cAGGTGGGACCACCGCGCTACTGCCACCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGANACCCCACACTAACATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCt                                                                                                                                   >  1:550355/1‑300 (MQ=255)                               aGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCtt                                                                                                                                  >  1:677883/1‑300 (MQ=255)                                                 aCTGCCGCCAAACAAATTCTTTTCTACTCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGCGTCGAACTGGGGACTTCACCCTCACCAAGGGTGCGCTATACCAAATGAGCCATATCAGCACGCTAAATTTGCTGCCTGGCAGTTCCCTACTCTCGCATGNGGAGACCCCACACTAACATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGCGGGACCACCGCGCCACTGCCGCCGAGCGAATTCTGTTTTATCAGACAGCTTATACGTTCTGATTTAATc                                                                                                                >  2:188848/1‑300 (MQ=21)                                                         cAGACAAATCCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAg                                                                                                        <  1:364785/300‑1 (MQ=255)                                                           gACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGATTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGc                                                                                                      <  1:378951/300‑1 (MQ=255)                                                           gACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGc                                                                                                      <  1:60827/300‑1 (MQ=255)                                                                                    aCTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCaa                                                                            >  2:8960/1‑300 (MQ=255)                                                                                                         cTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTANTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAgg                                                       >  1:572289/1‑300 (MQ=255)                                                                                                                   gagTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCtaa                                             >  2:346325/1‑298 (MQ=255)                                                                                                                             ggggACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTa                                   >  1:755776/1‑300 (MQ=255)                                                                                                                              tggatcTCACCCTTACCAAGGGTGCGCGCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATTGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAg                                  <  2:12269/295‑1 (MQ=255)                                                                                                                              gggACCGCACCCTTACTAAGGGTGCGCTCTACCACCTGAGCCATATCAGCACGCTAGATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACTGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAg                                  <  2:457514/300‑1 (MQ=14)                                                                                                                                  ccTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTNGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CANTCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCATCACGGTTCATTAGTAcc                              >  2:719649/1‑300 (MQ=255)                                                                                                                                     cACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGt                           >  1:76670/1‑300 (MQ=255)                                                                                                                                      gacagtACCAAGGGTGCGCTCGACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGtt                          <  2:323173/295‑1 (MQ=255)                                                                                                                                       cccTTACCAAGGGTGTGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTa                         >  1:1324/1‑300 (MQ=255)                                                                                                                                       cccTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGACAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CACTCCGCCAAAACAACTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTa                         >  2:457185/1‑300 (MQ=255)                                                                                                                                         cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCAAGGTTCATTAGTACCGGTTAGc                       >  2:476033/1‑300 (MQ=255)                                                                                                                                         cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGc                       >  2:78379/1‑300 (MQ=255)                                                                                                                                         cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGc                       >  1:150854/1‑300 (MQ=255)                                                                                                                                         cTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTAT‑CAATCCGCCAAAACACCTTCGGCGTTGTCAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGc                       >  2:80961/1‑300 (MQ=255)                                                                                                                                           tcccAAGGGTGCACTCTATCAACCGAGCCCTATCAGCATGCTAAATTTGATGCATGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAATATCGGAGCTAAGGCGTTCCACTTCTGAGGTCGGCATGGGGTCGGGTGGGACCATCGCGATACTGCCGCAAGGCAAATTATGTTTTCTCGGGCCGCTTCTGCGTTCTGATTTAGCCTGTATCAGGCTGACAATCTTCCCT‑CACTCCGCCAAAAAACCTTAGGCGTCGTAAGGCTAACCCTCACAGTTCATTAGTACCGTTTAGCTc                     >  2:45658/3‑300 (MQ=2)                                                                                                                                           tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGttctgtc                     >  1:325162/1‑295 (MQ=255)                                                                                                                                           tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTc                     >  1:295895/1‑300 (MQ=255)                                                                                                                                           tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTc                     >  2:345540/1‑300 (MQ=255)                                                                                                                                           tACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACCCTCGCATGGGGAGACCCAACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTTGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTAT‑CAATCCGCCAAAACATCTTCGGTGTTGTCGGGTTAAGCCTCACGGTTCATTAGTACCGGTTCGCtt                     >  2:45735/1‑299 (MQ=14)                                                                                                                                                aGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGc                >  1:310631/1‑300 (MQ=255)                                                                                                                                                     gcgcTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGc           >  1:345439/1‑300 (MQ=255)                                                                                                                                                          ctACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTACA‑TCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTgcgc      >  1:145778/1‑300 (MQ=255)                                                                                                                                                          ctACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CAATCCGCCAAAACACCTTCGGCGTTGTAGGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGATgcgc      >  2:460779/1‑300 (MQ=255)                                                                                                                                                              cAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCAACACTACCATCGGCGCTAAGGCGTTTCACTTCTGAGTTCGGCATGGGGTCGGGTGGGACCACCGCGCTACTGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTATGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTAT‑CAATCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCACAACGGTTCATTAGTACCGATTAGCTCAACACATCGCTGCGCttcc  >  2:195861/1‑298 (MQ=11)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                   GCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCTAAAAAGTGGTGCTGATACCCAGAGTCGAACTGGGGACCTCACCCTTACCAAGGGTGCGCTCTACCAACTGAGCCATATCAGCACGCTAAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCT‑CA‑TCCGCCAAAACAGCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTAC  >  NC_000913/3423481‑3423936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑