breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,554,0000TG45.0% 23.2 / 11.7 41Y557S (TAC→TCC) maeAmalate dehydrogenase (oxaloacetate‑decarboxylating)
*NC_000913888,0140TG36.8% 53.7 / 14.5 38noncoding (44/79 nt)rybBsmall regulatory RNA RybB
*NC_0009132,667,1260AC36.4% 63.6 / 12.6 57Y241D (TAT→GAT) hcaTputative 3‑phenylpropionate transporter
*NC_0009132,296,7640TG35.6% 114.1 / 11.4 73L87F (TTA→TTCccmBcytochrome c maturation protein B
*NC_0009134,606,9020AG33.8% 123.1 / 16.2 77S267P (TCC→CCC) rsmC16S rRNA m(2)G1207 methyltransferase
*NC_0009133,813,2720CT30.3% 31.3 / 10.3 35S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
*NC_0009134,171,5570CG27.9% 70.2 / 40.6 44intergenic (+13/‑80)rrlB/rrfB23S ribosomal RNA/5S ribosomal RNA
*NC_0009131,174,6050TC25.0% 127.1 / 10.1 57G187G (GGA→GGGycfTinner membrane protein YcfT
*NC_0009131,466,2010TC24.0% 137.7 / 18.8 82pseudogene (810/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009133,199,9350AG23.8% 117.7 / 10.1 65I344T (ATC→ACC) ygiFinorganic triphosphatase
*NC_0009131,174,6260GA23.6% 114.7 / 12.6 55F180F (TTC→TTTycfTinner membrane protein YcfT
*NC_0009131,466,2760TC23.5% 127.7 / 12.0 72pseudogene (885/2518 nt)ydbAputative outer membrane protein, N‑terminal fragment
*NC_0009133,848,3330TA22.5% 173.0 / 14.6 74M98L (ATG→TTG) uhpCinner membrane protein sensing glucose‑6‑phosphate
*NC_0009133,946,6200AG22.4% 145.5 / 39.6 76intergenic (+13/‑80)rrlC/rrfC23S ribosomal RNA/5S ribosomal RNA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =47 (0.820)5 (0.100) 5/438 3.6 11.6% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 34 (0.660)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 42707140 (0.690)3 (0.050) 3/490 4.7 5.6% coding (935/1128 nt) tgt tRNA‑guanine transglycosylase
?NC_000913 = 2912107 61 (1.060)coding (1545/2235 nt) relA GDP/GTP pyrophosphokinase