Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,813,272	0	C	T	30.3%	31.3 / 10.3	35	S181L (TCG→TTG)	dfp	fused 4'‑phosphopantothenoylcysteine decarboxylase and phosphopantothenoylcysteine synthetase
Reads supporting (aligned to +/- strand):  ref base C (2/21);  new base T (10/0);  total (14/21)
Fisher's exact test for biased strand distribution p-value = 7.13e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTC  >  NC_000913/3813035‑3813521                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                 gggcgatggACCTGGTTTGGACGATTTGTCTGGCTACACCTGCCCCTTTCGCCTTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑Ac                                                                                                                                                                                                                                                   <  1:274601/243‑1 (MQ=255)     gAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAGCGCA‑CTTTTTGCCCGT‑ccaccgcccg                                                                                                                                                                                                                                                 >  1:233516/1‑241 (MQ=255)                tATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGGGCA‑TTTTTTGCCCGT‑ACACG‑ACCCGAAA‑AAACT‑Gaa                                                                                                                                                                                                                                   >  1:185251/1‑250 (MQ=255)                 aTCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCC‑TTTTTTGCCCGT‑CAACG‑ACCCGAAA‑AATCT‑GAAc                                                                                                                                                                                                                                  >  1:109005/1‑250 (MQ=255)                                      acCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACAAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCACGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGAACGGG‑CTTTTTGCCAGG‑CAACG‑ACCTGAAAACATCtcacaataatattacagcccgcccg                                                                                                                                                                                                               >  1:183229/1‑226 (MQ=255)                                       cctccacgtttgccctcgCTCCCCGGCAGTAACCAGCAGTTGTACCGTGCCGCTGCCCCGCAGCATAATTTAGAGGTGTTTGCTTCCCTTGGTTTGCTCATCGGGGGGCCGGACATTGGCATTCGGGTTTGTGGTGTTATCGGTCCTGGGCGAATGCTCGACCCGTTAACCATTGTGGATATGGCGGTAGCTCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGAc                                                                                                                                                                                                          <  2:56232/234‑1 (MQ=255)                                              cgttgcctgtcccccccGCCATGAACCAGCAGATGTACCGTGCCGCGGCCACGCGGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGGCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGAGCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGaa                                                                                                                                                                                                   <  1:33397/238‑1 (MQ=255)                                                gtcccgctgccccccGCCATGAACCAGGAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTTCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAAcc                                                                                                                                                                                                 <  1:155714/240‑1 (MQ=255)                                                gaggccttgacccccGCCAGGAACCAGAAGATGGACCGTGCCGCGGCCACGCAGCATAATTTAGAGGGGCTTGCTTCCCGTGGTTGGCTCATCTGGGGGCCAGACAGTGGCAGGCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTATCCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAC‑CATCT‑GAACATTATGATTTC‑CGCCGGCCCGACGCGTGAAcc                                                                                                                                                                                                 <  1:231122/240‑1 (MQ=255)                                                  cgCCGTGCCCCCCGCCATGACCCAGCAGATGTACCGTGCCGCTGCCCCGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑CCCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGc                                                                                                                                                                                               <  1:67312/250‑1 (MQ=255)                                                       ccccccccGCCAGGAACCACCAGAGTTACCGTGCCGCTGCCCCGCAGCATAATTGAGGGGTGCTTGCTTCCCGTGTTTTTCTCATCTGGGGGCAAGACAGGGGCAGTCAGGCTTGTGGTGATATCGGTCCGGGGCGAATGCTCGTTCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑CCCTGAAC‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAt                                                                                                                                                                                          <  2:329/247‑1 (MQ=255)                                                                      accaccagagttACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTCCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATAtc                                                                                                                                                                          <  1:200174/240‑1 (MQ=255)                                                                         cgcagATGTCCCGTGCCCCTGCCCCGCAGCTTAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATctct                                                                                                                                                                       <  1:351861/250‑1 (MQ=255)                                                                           cagATGTCCCGTGCCGCTGCCCCGCAGCATAATTTAGAGGTGCTGGCTCCCTGGGGTTGGCGCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGGGGTGATATCGGTCTTGGGTGAATGCTCGATCCGTTAACCATGGTGGATAGGGCGGTAGCGCA‑TTTTCCGCCCGT‑CAACG‑ACCTGAAA‑TATCT‑GAACATTAGGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTaa                                                                                                                                                                     <  1:270677/251‑1 (MQ=255)                                                                             gATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGGGGGGGACTTTTTGGCCC‑T‑CAACGCACCTGAAA‑‑AACT‑GGACATTCTGATTACACGCCG‑CCCGGCGCGTGACACGCTCgcccccgggcgcgagaactctact                                                                                                                                                                     >  2:58985/1‑227 (MQ=255)                                                                                    cGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGGGGC‑TTTTTTGCCCCT‑CCACA‑ACCTTAAA‑AATCC‑TAACATTATTATTAA‑CGCCCGCCCGCCGCGTGCAACGCTCCAT‑CCCGGGCGTTTCTTCTCTAATcacaaccc                                                                                                                                                            >  1:63252/1‑247 (MQ=255)                                                                                         cGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTTTCCCGC‑CAACG‑CCCTCAAA‑CATCTTGAA‑ATTTTGATTAC‑CGCCGGCCCGACCCGTGAACCCCTCGATTCCGGTG‑GGTATATCTCTAATtacaagcccggc                                                                                                                                                        >  1:77408/1‑242 (MQ=255)                                                                                          gCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTTGCCCCG‑CAACG‑ACCCTAAA‑CATCT‑GAACATTATTATTAC‑CGCCGGCCCCGCGCGTGAACCGCCCGAT‑CCGGTGCGCTTTATCTCTAATCACAGCTCCgggc                                                                                                                                                       >  1:290974/1‑248 (MQ=255)                                                                                                                gggTGCTGGCTTCCCGTGGTTTGCTATTCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCGGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑CCCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATcgccgcc                                                                                                                                <  1:231831/250‑1 (MQ=255)                                                                                                                                 ggTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGATCGATCCGTTAACCATTGTGGATATGGCGGGAGCGGA‑TTTTTTGCCCGC‑CAACG‑AACTGAAA‑CAACT‑GAAAAATATGATTAA‑CGCCGGGCCCACGCGGGGAACGCTCGAA‑CCGGTGCGTTTTTTCTCTAATCACAACTCCCGCGAGATGGGTTTTGTTATACCCGCCGCCGCTGCCCCTCGTgg                                                                                                               >  1:306853/1‑251 (MQ=255)                                                                                                                                                         aGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑ATTTTTGCCCGC‑CAAAG‑ACCTGAAA‑CATAT‑GAAAATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCACGAT‑CCGGGGCGGTATATCTCTAATCACAACTCCGGCAAGATGGGGTTTGCGATCGCCGCCGCCGCGGCCCCGCGCGGCGCGAACggtacgccgggttcagg                                                                                       >  1:16365/1‑235 (MQ=255)                                                                                                                                                                         gtggtgttatgggctcTGGGCTAATTCTCGTCCCGTAAACCATTGTGGATATGGCGGT‑GCCCC‑TGTTTCCCCCGGGCAACG‑CCCTGAAA‑CATCT‑GAACGTTATGATTAC‑CGCCGTCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCGCTAATCACAGCTCCGGAAAGATGGGTTTTGCTATCGCCTCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTAcc                                                                       <  2:315542/235‑1 (MQ=255)                                                                                                                                                                           ggtgATTTCGGTCCGGGGGGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTGGCGCT‑TTTTCCGCCCGT‑CAACG‑CCCTAAAA‑CATCT‑GAACTTTAGTATTCC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTAGCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGAc                                                                    <  2:124920/251‑1 (MQ=255)                                                                                                                                                                           ggggATATCGGTCCTGGGCGAATGCTCGGTCNGTTAACCATGGTGGATATGGCGGTAGCGCT‑TTTTTCGCCCGC‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTGTCACTACCGAc                                                                    <  2:232387/248‑1 (MQ=255)                                                                                                                                                                                 accGGTCCGGGGGGAAGGCTCGACCCGTTAACTATTTTGAAAATGGCGGAAGCGAA‑TTTCCCGCCCGT‑CAACG‑ACTTAAAA‑CAGCG‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCGCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGGCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCAcc                                                              <  1:58985/249‑1 (MQ=255)                                                                                                                                                                                       ccggggCGAATGCGCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTAGCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGt                                                        <  2:109005/248‑1 (MQ=255)                                                                                                                                                                                            gCGAATGCTCGATCCGTTAACCTTTGGGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAAc                                                   <  1:349377/251‑1 (MQ=255)                                                                                                                                                                                               aaTGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCC‑TTTTTTGCCCCT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGTTTAC‑CCCCGGCCCGGCGCGTGAAACGCCCGAT‑ACGGGGCGTTATATTCCTAATCACAACTCCCGCCAGATGGGGTTTTCTTTCGCCCCCGCCGCCGCCCGTCGTGGGGCGGACGTCACGCCGGTATCCGGGC‑CGGGTTCACCACCCACGCCACCCTTTGTTAAACggg                                                >  1:381769/1‑249 (MQ=255)                                                                                                                                                                                                  gtccgcgccGTTACCCATGGTGGATGTGGGGGGAGCGCA‑TTTTGCCCCCGT‑CACCG‑ACCTGAAA‑CATCT‑GACAATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGAGGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGtt                                              <  2:381769/243‑1 (MQ=255)                                                                                                                                                                                                                  aTTGTGGATATGGCGGTAGCGCT‑TTGTCCGCCCTT‑CAACG‑ACCTGAAA‑CATCT‑GAACTGTATGATTAC‑CGCCGTCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCt                             <  2:63252/251‑1 (MQ=255)                                                                                                                                                                                                                   tgttggaagtGGCGGTGGCGTT‑TTTTTCGCCGGC‑CAACG‑ACTAGAAC‑CATCG‑GACCATTATGGTTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCt                             <  2:371183/241‑1 (MQ=255)                                                                                                                                                                                                                        gATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CACCT‑GAACATTAGGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAAt                       <  1:61642/251‑1 (MQ=255)                                                                                                                                                                                                                           atGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGCTTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGGGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGGTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGGATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGGGGTGATGTGATGACCGCGCTGGAAATgg                     >  1:276553/1‑250 (MQ=255)                                                                                                                                                                                                                                  tAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAgccgcc             >  1:298852/1‑251 (MQ=255)                                                                                                                                                                                                                                          tttttAGCCCGG‑CAACG‑ACCTGAAA‑CATCT‑GCACATTATGATTAC‑AGCCGGCCCGACGCGTGAACAGCTCGAT‑CCGGCGCGTTATATCTCTAATCACAGCGCCGGCAAGATGGGTTTTGCTATCGCCGCCTCCGCTGCCCGTCGTGGCTCGAACGTCACGCTGGTATCAGGGCTCGGCT‑CACTACCGACCCCACCGTTTGCTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGTAGCCGCAGTGactg      >  2:466146/1‑248 (MQ=255)                                                                                                                                                                                                                                              ccgcccgc‑cAACG‑ACTTGAAA‑CATCT‑GAACTTTTTGTTTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTc  <  1:359280/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                 TGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCTTCCCGTGGTTTGCTCATCTGGGGGCCAGACAGTGGCAGTCAGGCTTGTGGTGATATCGGTCCTGGGCGAATGCTCGATCCGTTAACCATTGTGGATATGGCGGTAGCGCA‑TTTTTCGCCCGT‑CAACG‑ACCTGAAA‑CATCT‑GAACATTATGATTAC‑CGCCGGCCCGACGCGTGAACCGCTCGAT‑CCGGTGCGTTATATCTCTAATCACAGCTCCGGCAAGATGGGTTTTGCTATCGCCGCCGCCGCTGCCCGTCGTGGCGCGAACGTCACGCTGGTATCAGGTC‑CGGTTTCACTACCGACGCCACCGTTTGTTAAACGTGTTGATGTGATGACCGCGCTGGAAATGGAAGCCGCCGTGAATGCTTC  >  NC_000913/3813035‑3813521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑