Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,848,333	0	T	A	22.5%	173.0 / 14.6	74	M98L (ATG→TTG)	uhpC	inner membrane protein sensing glucose‑6‑phosphate
Reads supporting (aligned to +/- strand):  ref base T (36/19);  new base A (0/16);  total (37/37)
Fisher's exact test for biased strand distribution p-value = 1.27e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTT  >  NC_000913/3848085‑3848576                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         aTGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATa                                                                                                                                                                                                                                                         >  1:297715/1‑250 (MQ=255)   gcagcgcagcCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATaaa                                                                                                                                                                                                                                                       >  1:92829/1‑250 (MQ=255)        gaagcCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGAGGTTATGCGCCGTGTCCCATAATGCCCACCACCCGCCGCGCTCGGTACGTGAATACCATGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACg                                                                                                                                                                                                                                                 <  1:440430/249‑1 (MQ=255)           gcCGTTGCCATAACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGc                                                                                                                                                                                                                                              <  1:202560/251‑1 (MQ=255)            ccGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAACAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCACAAAATAACGGGc                                                                                                                                                                                                                                              >  2:126128/1‑250 (MQ=255)                   cATCACAATGGTAATGAGTGAGCCGCCGACGTTATGCGCCGGGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGc                                                                                                                                                                                                                                     <  1:200561/251‑1 (MQ=255)                        caaggggAATGAGTGCCCCGCCGACGTGAGGCGCCGGGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATCCCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑TCGGCGATAAG‑CCCTAGCCCCATAAAATAACGGGCATTTG‑AGCGATC‑g                                                                                                                                                                                                                               <  2:120995/247‑1 (MQ=255)                           tGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTg                                                                                                                                                                                                                            >  1:123426/1‑251 (MQ=255)                           ggggaatggttccgccgcCGACGTTATGCGCCTTGTCCCATAAGGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGGTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTTGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GAGAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑TGCTATC‑GCTg                                                                                                                                                                                                                            <  2:121504/239‑1 (MQ=255)                                atgggTGCCCCGCCGACGTTAGGCCCCGGGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑t                                                                                                                                                                                                                      <  2:420416/247‑1 (MQ=255)                                      gcgcCCCCGACGTTAGGCGCCTTGTTCCAAAATGCCACCCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGGCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGga                                                                                                                                                                                                                <  2:120888/251‑1 (MQ=255)                                           gccgACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACa                                                                                                                                                                                                           >  2:142192/1‑250 (MQ=255)                                                 ggtaggcgcCGTGTTCCATAATGCCCCCCATCCGCCGCGCTCGGTACGTGAAGACCAGGCCGTTAACAGACGCGCACACACC‑GGGGAACCCCAGCCCTGGAAAAAGGCGTTCAGCCCCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAATAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTGTCCCCAGAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCg                                                                                                                                                                                                    <  2:135019/246‑1 (MQ=255)                                                      gcgcCTTGTTCCATAACGCCCCCCAACCGCNGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑acac                                                                                                                                                                                               <  2:230678/250‑1 (MQ=255)                                                      gcgcCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACg                                                                                                                                                                                              >  1:350504/1‑251 (MQ=255)                                                               cccaaaatcccccccaACCGCCGCCCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCCCCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTa                                                                                                                                                                                     <  2:180556/238‑1 (MQ=255)                                                                    aacgCCCACCACCCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGTGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTa                                                                                                                                                                                <  2:300073/248‑1 (MQ=255)                                                                         ccaccaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GTTGAACCCCACCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGTA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATCACGGGCATTTG‑AGCGGTC‑GCTGACAC‑TGCCGGAG‑ACAAACTTCG‑GCCCGCCATAGGTACTGTAAAAcc                                                                                                                                                                           >  2:181699/1‑250 (MQ=255)                                                                          caccaACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGCAAAAc                                                                                                                                                                            >  1:417638/1‑249 (MQ=255)                                                                               aCCGCCGCGCTCGGTACGTGAATACCAGGCCGTGAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAACAGGCGTTAAGCACCCAGAGCACGGCAAACGCAC‑ATAGCGACGCCGGGAA‑GCCACAAAGAATGTT‑GATAATGCCC‑GGGGCGATAAG‑CACTATACCCATAAACTAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑AAAAACTTCG‑ACACGCCATAGGTAGTGTAAAACAGGGTCg                                                                                                                                                                     >  2:200405/1‑251 (MQ=255)                                                                                  gccgcGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCt                                                                                                                                                                   >  2:345615/1‑250 (MQ=255)                                                                                            gTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGat                                                                                                                                                        >  1:405161/1‑251 (MQ=255)                                                                                                   aaTACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCgat                                                                                                                                                  >  1:408143/1‑248 (MQ=255)                                                                                                    atcccAGGCCGTTAACAGACTCGC‑CACCCCCGGTGACCCCCAGCCCGGGAAAAAGGCGTGCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGACATCGCTAc                                                                                                                                                <  2:322462/248‑1 (MQ=255)                                                                                                    aTACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTAc                                                                                                                                                >  2:22327/1‑251 (MQ=255)                                                                                                       ccAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGc                                                                                                                                             >  2:143220/1‑251 (MQ=255)                                                                                                           gCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAg                                                                                                                                         <  1:451559/251‑1 (MQ=255)                                                                                                                           cacccgcc‑GGGGAACCCCGGCCCGGGAAAAGGGGGTTCAGCACCGGGAGCGCGGCAACCCCCC‑AGGGGGACGTCGAGAA‑GCCAAACAGAGGGTG‑GAAAAGGCCC‑GGGGCGATAGG‑CCCAACCCCCAAAAAAAAACGGGCTTTGG‑GGGGATC‑GTGGACAG‑GGCCGGAG‑ACAAGCTGCG‑CCACGCAATAGGAAATGTAAAACAGGGTCGCGAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAgg                                                                                                                         <  2:42114/245‑1 (MQ=255)                                                                                                                             cacacC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCg                                                                                                                                                                                                    <  1:139588/175‑1 (MQ=255)                                                                                                                             cacacC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCg                                                                                                                                                                                                    >  2:139588/1‑175 (MQ=255)                                                                                                                                   ggttGAACCCCACCCATGGAAAAAGGCGTTACGCACCAAGACCACG‑CAAACCCCCCATAGCGACGTCGAAAA‑GCCAAAAAGA‑TGTTAGAAAAGGCCG‑GTGGCGAAAAG‑CCCTACCCCAAAAAAATATCGGGCTTTTG‑CGCGAGC‑GCTGACAA‑GGCCGGAG‑ACAACCTCCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGGGAACGCCGTTAGCAAGGATTTCTg                                                                                                                  <  2:92829/248‑1 (MQ=255)                                                                                                                                       aacccccAGCCCTGGAAAAAGGCGTTCAGCACCCAGACCACGCCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAAAATGTG‑GATAATGCCC‑GTGGCGAAAAG‑CCCTATCCCCAAAAAAAAACGGGCATTTG‑AGCAATC‑GCTGAAAA‑TGCCGGAG‑ACAACCTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTAc                                                                                                              <  1:257060/248‑1 (MQ=255)                                                                                                                                          ccccAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTAcggc                                                                                                           >  1:410604/1‑251 (MQ=255)                                                                                                                                           cccAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTTGNCACGCCGTTATCAAGGATTTCTGGTAcggcg                                                                                                          >  2:220898/1‑251 (MQ=255)                                                                                                                                           cccACCCCTGGAAAAGGGCTTTCAGCCCCCAGAGCACGGCAAACGCCC‑ATAGCGCCGTCGAGAA‑GCCAAACAGAATGTT‑GATAAGGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATACCGGGCATTTG‑AGCGACC‑GCTGACAT‑GGCCGGAG‑AAAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTAcggcg                                                                                                          <  1:440367/251‑1 (MQ=255)                                                                                                                                            ccAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTAcggcgg                                                                                                         >  1:10561/1‑251 (MQ=255)                                                                                                                                               gCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACCGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGgcggcgg                                                                                                      >  2:151791/1‑250 (MQ=255)                                                                                                                                                cccTGGAAAAAGCCGTTTGGCACCCAGAGCACGGAAAACGCCC‑ATAGCGGCGACGAGAA‑GCCAAACAGAATGTT‑AATAAGGCCC‑GTGGCGAAAAG‑CCCTACCCCAATAAAAAACCGGTCATTTG‑AGCGAGC‑GCTGAAAA‑TCCCGGAG‑ACAA‑CGTCGGACACGCCAGAGGTAATGTAAAACAGGGCCGCTAACAGGCCGATATCGCTCCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGtt                                                                                                     <  1:71522/251‑1 (MQ=255)                                                                                                                                                   tGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTaa                                                                                                   >  2:338134/1‑250 (MQ=255)                                                                                                                                                    ggAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCAAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCCAGGATTTCTGGTACggcggcagtaaa                                                                                                  >  2:462880/1‑244 (MQ=255)                                                                                                                                                    ggAAAAAGACGTTCACCCCCCAGAGCACGCCAAACCCCT‑ATAGCGCCGTCGAAAC‑CCCAAACAGAATGTT‑GATAATGCCC‑GGGGCAATAAG‑CCCCTTCCCCAAAAAAAAACGGGCATTTG‑AGCGATC‑GCTGACAG‑TCCGGGAA‑ACAAACTCCG‑ACACGCCATGGGTAATGTAAAACAGGGTCGCTAACAGGCCGTTACCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTaaaa                                                                                                 <  1:296756/251‑1 (MQ=255)                                                                                                                                                         aaggagttcagcccccaaacaccgccAAACGCCC‑ATACCCACGTC‑AGAAACCCAAACGGAATTTG‑GATAATCCCC‑TTGCCGATAAG‑CCCTATCCCCATAAAAAAAGGGGCTTTTG‑GGCGACC‑TCTGACAT‑TGCCGGAG‑ACAATCTTCG‑ACACGCCATTGGTAATGTAAAACAGGTTCGCTAACAGGCCGATTTCCCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCTGCGTTAAAACtttt                                                                                            <  1:221389/226‑1 (MQ=255)                                                                                                                                                             cgtccAGCACCCAGAGAACGCCAAACGCCA‑ATAGCGCCGTCGAGAA‑GCAAAAAAAAATTTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCAAAAAAAAAACGGGCTTTGG‑AGCAATC‑GTTAACAA‑GGCCGGAA‑ACAAATTACG‑ACACCCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTCCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCcgc                                                                                        <  1:252868/247‑1 (MQ=255)                                                                                                                                                              gTTCAGCACCCAGAGCCCGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATTTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAACCTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCcgcg                                                                                       <  1:112785/251‑1 (MQ=255)                                                                                                                                                                  aGCACCCAGAGCACGGCAAACGCCC‑ATGGCGACGTCGAAAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCAAAAAATAACGGGCATTTG‑AGCGATC‑GCTGAAAA‑TGCCGGAG‑ACAACCTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGaa                                                                                   <  1:26036/251‑1 (MQ=255)                                                                                                                                                                  aGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGa                                                                                    >  1:101289/1‑250 (MQ=255)                                                                                                                                                                  aGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGa                                                                                    >  1:137093/1‑250 (MQ=255)                                                                                                                                                                    cACCAAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCAAAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGt                                                                                 <  1:115391/251‑1 (MQ=255)                                                                                                                                                                     aCCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGCGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGt                                                                                 >  1:80085/1‑250 (MQ=255)                                                                                                                                                                               cGGCAATCGCCA‑ATAGCGAGGTCGAGAA‑GCCAAACAGAATGTG‑GATAAGGCCC‑GTGGCGATAAG‑CCCTACCCCCAAAAAATAACGGGCATTTG‑AGCGATC‑GCTGAAAA‑GGCGGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGcgcg                                                                      <  1:383549/251‑1 (MQ=255)                                                                                                                                                                               cGGCAAACGCCA‑ATAGCGACGTCGAGAA‑GACAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGCG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGcgcg                                                                      >  1:129191/1‑251 (MQ=255)                                                                                                                                                                                ggCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTCTCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGCG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACCGGACGATATCGCTACGGCTGAGCACGCCGTTCGCAAGGATTTCTGGAACGGCGGCGTTAAAACTTTTCAGTCTGAAGGAAAACacggcgt                                                                     >  2:306012/1‑245 (MQ=255)                                                                                                                                                                                     aCGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAAccc                                                                >  1:21624/1‑251 (MQ=255)                                                                                                                                                                                     aCGCCC‑ATAGCGACGTCGAGAA‑CCCAAACAAAATGTT‑GAAAATGCCC‑GTGGCGATAAG‑CCCTATCCCCAAAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAAGGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAAccc                                                                <  1:126128/251‑1 (MQ=255)                                                                                                                                                                                           ccatagggaggtggacaacccAAACAGAATGTT‑GATA‑TGCCCCGGGGCGTAAAC‑CCCTATCCCCA‑AAAAAAACCGGCATTTGGAGCGTCC‑GCTGTCAC‑TGCGGGAA‑ACAATTTTGA‑ACACGCCTAAGGTAATGTAAAGCAGGGTCGCTGACAGGCCGATACCGCTACGGATGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACccagcc                                                            <  1:365474/232‑1 (MQ=255)                                                                                                                                                                                                      cGAGAT‑GCAAAACAGAATGTT‑GAAAATGCCC‑GGGGGGATAAG‑CCCTATCCCCATAAAAAAACGGGCATTTG‑AGCGAGC‑GCTAACAA‑GGCGGGAG‑ACAAACTTCG‑ACAGTCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAGACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTcagc                                                 <  1:257885/250‑1 (MQ=255)                                                                                                                                                                                                        agaA‑GCCAAACAGAATTTT‑AATAATGCCC‑GTGGCGATAAC‑CCCTATCCCCAAAAAAAACCGGGCATTTG‑AGCAATC‑GCTGACAA‑TCCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGa                                              <  1:464700/251‑1 (MQ=255)                                                                                                                                                                                                          aa‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATTGTCAGCAGAat                                            >  2:76309/1‑251 (MQ=255)                                                                                                                                                                                                                aaaCAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGCAGTAAAACAGCGCGTAACCCCGCCAGATGGTCAGCAGAATATGcc                                       >  1:416365/1‑251 (MQ=255)                                                                                                                                                                                                                 aacaaaaggTT‑GATAATGCCC‑GTGGCG‑TAAGGCCCTATCCCCAAAAAATAACGGG‑ATTTGGAGCGACC‑TCGGCCAA‑TGCCGGAG‑ACAACCTCCG‑ACACGCCATAGGTAATGTAAAACAGGGTGCCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACGTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCg                                      <  2:309307/243‑1 (MQ=255)                                                                                                                                                                                                                  aCAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCAATAAAATAACGGGCATTTG‑AGCGATC‑GCTGAAAA‑TGCAGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGATGAGCACGCCGTTAGCAAGGATTTATGGTACGGCGGCGATAAAACTTTTCCGCGGGAAGTAAAAAAGCGCGTAAACCAGCCAGATGGTCAGGAGAATATgacg                                      >  1:292631/1‑247 (MQ=255)                                                                                                                                                                                                                   cAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGAATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTACTGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGGTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAAAATGCCGa                                     >  2:408102/1‑250 (MQ=255)                                                                                                                                                                                                                      aaTGTT‑GATAATGCCA‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCCGACAGAAGGTCCGCGGAATATGCCGACGcc                                 >  1:231404/1‑251 (MQ=255)                                                                                                                                                                                                                              aTAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAACACTTTTCCGCGTGAAGTAAAAAAGCGCTTAACCCCGCCAGATGGTCAGCAGAATATGCCGACGACAGGAGCg                          >  2:418011/1‑251 (MQ=255)                                                                                                                                                                                                                                aaTGCCC‑GTGGCGATACG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACACACTTCG‑ACACGCCATCGGGAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGCTTTCTGGTACGGCGGCGGTAAAACTTTTCCGCGGGAAGGAAAACAGCGCGTAACCCACCCGAATGGTCAGCCGAATTAGCCGACCCCAGTAgagc                         >  2:24274/1‑247 (MQ=255)                                                                                                                                                                                                                                 aTGCCC‑GTGGCGAAAAG‑CCCTATCCCCAAAAAAAACCGGGCATGTG‑AGCGATC‑GCGGACAA‑TGCCGGAG‑ACAACCTCCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATa                       <  2:123426/251‑1 (MQ=255)                                                                                                                                                                                                                                   gCCC‑GTGGCGAAAAG‑CCCTCTCCCCAAAAAATAACGGTCATTTG‑AG‑GATCCGCTGA‑AA‑TGCCCGAGGACAACCGTCA‑CCCCGCCATAGGAAATGAAAAACAGGGTCGCTAACAGGGCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTTGTACGGCGGCGTTAAAACTTTTCCGCGTGAATTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAg                      <  1:411774/250‑1 (MQ=255)                                                                                                                                                                                                                                              aTAAG‑CC‑TATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGGAACCCAACCAGATGGTCACCAGAATATGACGACGCCAGTAGCGATAGCGGGCAtaactt          >  2:265271/1‑246 (MQ=255)                                                                                                                                                                                                                                               aaaG‑CCCAATCCCCAAAAAAAAACGGCCTTTTG‑AGCGACC‑GCTGCCAA‑GGCCGGAG‑ACAACCTTCG‑CCCCGCCATAGGTAATGTAAAACAGGGTCGCAAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAAtt          <  1:59718/250‑1 (MQ=255)                                                                                                                                                                                                                                                aaaccccgaTCCCCAAAAAATAACGGCAATTTG‑AGCGATC‑GCGGACAA‑GGCCGGAA‑AAAAATTGCG‑ACACGCCATAGGTAAGGTAAAACAGGGTCGCTAAGAGGCCGATACCGCTCCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAAtt          <  1:372494/243‑1 (MQ=255)                                                                                                                                                                                                                                                 aG‑CCCTATCCCCAAAAAATAACGGGCATTTG‑AGCGACC‑GCTGACAA‑TGCCGGAG‑ACAACCTTCG‑ACACGCCATAGGTAATGTAAAACAGGTTCGCTAACAGGCCGATATCGCTCCGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCTATAGCGGGCATCAATTTc        <  1:348353/251‑1 (MQ=255)                                                                                                                                                                                                                                                    cccTATCACCATAAAACACCGGGCATTTG‑GGCGACC‑GC‑GAAAACTGCCGGAA‑ACAAACTCCG‑ACACGCCATAGGTAAGGTAAAACAGGTTCGATAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCat      <  2:322321/251‑1 (MQ=255)                                                                                                                                                                                                                                                     ccTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGGAGCGATAGCGGGCATCAATTTCat      >  2:177783/1‑250 (MQ=255)                                                                                                                                                                                                                                                        aTCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCCATTTCATCttt  >  2:99510/1‑251 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                         ATGCAGCGCAGCCGCTGCCATCACAATGGGAATGAGTGCGCCGCCGACGTTATGCGCCGTGTTCCATAATGCCCACCAACCGCCGCGCTCGGTACGTGAATACCAGGCCGTTAACAGACGCGCACACACC‑GGTGAACCCCAGCCCTGGAAAAAGGCGTTCAGCACCCAGAGCACGGCAAACGCCC‑ATAGCGACGTCGAGAA‑GCCAAACAGAATGTT‑GATAATGCCC‑GTGGCGATAAG‑CCCTATCCCCATAAAATAACGGGCATTTG‑AGCGATC‑GCTGACAA‑TGCCGGAG‑ACAAACTTCG‑ACACGCCATAGGTAATGTAAAACAGGGTCGCTAACAGGCCGATATCGCTACGGCTGAGCACGCCGTTAGCAAGGATTTCTGGTACGGCGGCGTTAAAACTTTTCCGCGTGAAGTAAAACAGCGCGTAACCCAGCCAGATGGTCAGCAGAATATGCCGACGCCAGTAGCGATAGCGGGCATCAATTTCATATTT  >  NC_000913/3848085‑3848576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑