breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 172 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG37.1% 216.2 / 23.2 117G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_0009134,429,9840TG36.3% 199.1 / 17.5 102L41V (TTG→GTG) cycAD‑serine/alanine/glycine/:H(+)symporter
*NC_0009134,171,5570CG35.9% 146.2 / 127.1 92intergenic (+13/‑80)rrlB/rrfB23S ribosomal RNA/5S ribosomal RNA
*NC_0009133,844,2190TG35.1% 204.8 / 18.5 114F86V (TTT→GTT) adeDadenine deaminase
*NC_0009134,622,2500AG34.1% 174.1 / 28.3 91E161G (GAG→GGG) yjjJtoxin YjjJ
*NC_000913811,7210TG33.7% 492.1 / 52.7 295V67G (GTG→GGG) bioCmalonyl‑acyl carrier protein methyltransferase
*NC_0009134,296,2680TC33.3% 50.1 / 30.9 42intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,665,6980TC32.4% 65.9 / 11.9 37D38G (GAT→GGT) gadXDNA‑binding transcriptional dual regulator GadX
*NC_0009132,745,0890AC32.4% 313.3 / 35.8 148V84G (GTG→GGG) trmDtRNA m(1)G37 methyltransferase
*NC_0009134,126,2100TG32.1% 284.0 / 31.1 137T201P (ACC→CCC) priAprimosomal protein N'
*NC_0009131,911,7540TG32.0% 223.8 / 10.2 105T275P (ACC→CCC) htpXprotease HtpX
*NC_0009131,159,8370AG32.0% 249.4 / 40.9 131I572T (ATC→ACC) fhuEferric coprogen/ferric rhodotorulic acid outer membrane transporter
*NC_0009133,745,3740TG32.0% 142.3 / 13.7 101W191G (TGG→GGG) yiaN2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
*NC_0009131,491,0980AC31.5% 203.4 / 15.2 92Y66S (TAC→TCC) cybBsuperoxide oxidase
*NC_0009134,033,6120TG31.3% 247.8 / 13.9 116G156G (GGT→GGGtrkHK(+) transporter TrkH
*NC_0009131,383,7220GA30.8% 87.9 / 18.7 52F80F (TTC→TTTycjWDNA‑binding transcriptional repressor YcjW
*NC_0009131,174,6050TC30.5% 324.5 / 37.1 151G187G (GGA→GGGycfTinner membrane protein YcfT
*NC_0009133,751,4560AC30.4% 199.6 / 11.5 94V138G (GTG→GGG) yiaTouter membrane protein YiaT
*NC_000913786,8550AC30.4% 347.5 / 24.9 149G247G (GGT→GGGgpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
*NC_0009134,630,3400AC30.3% 232.8 / 24.2 126G61G (GGT→GGGettAenergy‑dependent translational throttle protein EttA

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 15911NA (NA)12 (0.080)
+TGTTTTCAA
11/470 3.7 NA noncoding (525/1345 nt) IS186 repeat region
?NC_000913 15922 = NA (NA)noncoding (536/1345 nt) IS186 repeat region
* ? NC_000913 3984447 =131 (0.860)3 (0.020) 3/478 5.8 1.9% coding (1561/1656 nt) aslA putative sulfatase AslA
?NC_000913 = 4266676 182 (1.210)coding (895/1080 nt) alr alanine racemase 1
* ? NC_000913 = 3693908252 (1.650)3 (0.020) 3/488 5.8 1.9% coding (1329/2619 nt) bcsA cellulose synthase catalytic subunit
?NC_000913 3853763 = 61 (0.400)intergenic (+121/+3) tisB/ysdE membrane‑depolarizing toxin TisB/protein YsdE
* ? NC_000913 = 3489724230 (1.500)3 (0.020) 3/488 5.8 2.0% coding (457/1221 nt) argD N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
?NC_000913 = 3671581 68 (0.440)coding (314/603 nt) yhjB putative DNA‑binding transcriptional regulator YhjB
* ? NC_000913 = 233510795 (0.620)3 (0.020)
+C
3/486 5.8 4.9% coding (1538/1689 nt) yfaA DUF2138 domain‑containing protein YfaA
?NC_000913 = 4603146 21 (0.140)intergenic (‑288/‑331) yjjP/yjjQ putative succinate exporter YjjP/DNA‑binding transcriptional repressor YjjQ
* ? NC_000913 = 1786961105 (0.690)3 (0.020) 3/488 5.8 1.7% coding (152/2379 nt) ppsA phosphoenolpyruvate synthetase
?NC_000913 2583975 = 248 (1.620)coding (1551/1980 nt) aegA putative oxidoreductase AegA
* ? NC_000913 986466 =253 (1.650)3 (0.020) 3/486 5.8 1.3% coding (517/1089 nt) ompF outer membrane porin F
?NC_000913 4477761 = 190 (1.250)coding (551/1005 nt) argI ornithine carbamoyltransferase ArgI
* ? NC_000913 262834 =132 (0.860)3 (0.020) 3/486 5.8 1.7% intergenic (+78/‑37) proA/thrW glutamate‑5‑semialdehyde dehydrogenase/tRNA‑Thr
?NC_000913 3195663 = 212 (1.390)coding (1091/1434 nt) hldE fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase