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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,604,350 | 0 | C | T | 46.2% | 18.9 / 14.0 | 26 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 3,665,702 | 0 | C | A | 41.9% | 28.1 / 14.3 | 31 | A37S (GCG→TCG) | gadX | DNA‑binding transcriptional dual regulator GadX |
* | NC_000913 | 1,022,915 | 0 | T | G | 41.9% | 46.6 / 10.1 | 43 | A323A (GCA→GCC) | yccS | putative transporter YccS |
* | NC_000913 | 4,296,268 | 0 | T | C | 40.0% | 21.6 / 22.8 | 25 | intergenic (+474/+168) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 4,604,344 | 0 | C | T | 38.5% | 25.4 / 11.2 | 26 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 4,056,115 | 0 | A | C | 38.3% | 73.1 / 11.6 | 48 | G75G (GGT→GGG) | glnL | protein histidine kinase NtrB |
* | NC_000913 | 2,434,882 | 0 | T | G | 38.0% | 83.6 / 11.2 | 50 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
* | NC_000913 | 2,326,450 | 0 | T | G | 37.5% | 89.7 / 13.8 | 56 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 941,713 | 0 | T | G | 34.9% | 121.4 / 20.2 | 64 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase subunit A |
* | NC_000913 | 3,665,698 | 0 | T | C | 33.3% | 44.0 / 10.3 | 31 | D38G (GAT→GGT) | gadX | DNA‑binding transcriptional dual regulator GadX |
* | NC_000913 | 4,171,557 | 0 | C | G | 33.3% | 60.4 / 46.8 | 45 | intergenic (+13/‑80) | rrlB/rrfB | 23S ribosomal RNA/5S ribosomal RNA |
* | NC_000913 | 3,177,748 | 0 | C | A | 32.5% | 56.1 / 11.5 | 41 | E55* (GAG→TAG) | nudF | ADP‑sugar pyrophosphatase |
* | NC_000913 | 811,721 | 0 | T | G | 32.0% | 125.8 / 12.0 | 80 | V67G (GTG→GGG) | bioC | malonyl‑acyl carrier protein methyltransferase |
* | NC_000913 | 3,556,207 | 0 | T | C | 31.7% | 76.2 / 12.0 | 41 | S215G (AGT→GGT) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 3,556,200 | 0 | G | A | 29.3% | 66.7 / 12.7 | 41 | S217F (TCC→TTC) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 4,482,648 | 0 | A | C | 28.6% | 132.1 / 11.1 | 70 | V397G (GTT→GGT) | valS | valine‑‑tRNA ligase |
* | NC_000913 | 2,185,199 | 0 | T | G | 28.2% | 172.0 / 10.6 | 78 | T35P (ACC→CCC) | thiM | hydroxyethylthiazole kinase |
* | NC_000913 | 4,223,644 | 0 | C | T | 25.5% | 108.4 / 11.3 | 47 | intergenic (‑16/‑184) | iclR/metH | DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase |
* | NC_000913 | 941,727 | 0 | G | T | 25.4% | 149.1 / 10.1 | 67 | E257* (GAA→TAA) | dmsA | dimethyl sulfoxide reductase subunit A |
* | NC_000913 | 2,185,238 | 0 | A | G | 25.0% | 195.9 / 12.4 | 80 | S22P (TCC→CCC) | thiM | hydroxyethylthiazole kinase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1411899 = | 57 (0.970) | 4 (0.080) | 4/442 | 4.2 | 9.9% | coding (50/936 nt) | ttcA | tRNA cytosine(32) 2‑sulfurtransferase TtcA |
? | NC_000913 | = 1434958 | 22 (0.420) | intergenic (‑552/+227) | ynaM/uspF | protein YnaM/universal stress protein F |