Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,223,6440CT25.5% 108.4 / 11.3 47intergenic (‑16/‑184)iclR/metHDNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base C (5/30);  new base T (12/0);  total (17/30)
Fisher's exact test for biased strand distribution p-value = 1.18e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAC  >  NC_000913/4223396‑4223887
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        
tCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCt                                                                                                                                                                                                                                                         <  1:332053/250‑1 (MQ=255)
    ccaCCTGACGCCCGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTCCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGT‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATc                                                                                                                                                                                                                                                   <  2:391089/249‑1 (MQ=255)
            cGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAAt                                                                                                                                                                                                                                           <  1:53410/251‑1 (MQ=255)
                   aaCCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCCCGTTTCTCGGGGA‑TGGG‑GGGGGCCATGA‑CAGTCTTTTTTTTTT‑GTATCGTGGGAAtcattttt                                                                                                                                                                                                                                    >  1:174589/1‑250 (MQ=255)
                    aCCCTGCTGTTGCGTCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAAtcattttca                                                                                                                                                                                                                                   <  1:341772/251‑1 (MQ=255)
                            gTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAGCCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTAtt                                                                                                                                                                                                                           <  2:164059/251‑1 (MQ=255)
                            gTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTAt                                                                                                                                                                                                                            <  2:328446/250‑1 (MQ=255)
                               gCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGt                                                                                                                                                                                                                         <  1:142878/250‑1 (MQ=255)
                                              ggCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGt                                                                                                                                                                                                        <  1:48653/251‑1 (MQ=255)
                                                cGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTa                                                                                                                                                                                                      <  1:33986/251‑1 (MQ=255)
                                                    gggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGTG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTactga                                                                                                                                                                                                  <  2:108153/251‑1 (MQ=255)
                                                    gggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTactg                                                                                                                                                                                                   <  1:332126/250‑1 (MQ=255)
                                                     ggTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTactgaa                                                                                                                                                                                                 <  1:305165/251‑1 (MQ=255)
                                                        cGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGCTTCGCGGGGA‑TGGG‑GGCGGCCATGA‑CAGGCTCTTTTTTTT‑GTATCGGGGGAATCATTTTTATTTTTTTTTTT‑AGCTAAATCAATAGtttatgaaatg                                                                                                                                                                                              >  1:426247/1‑242 (MQ=255)
                                                                 gggTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATg                                                                                                                                                                                      <  1:279831/250‑1 (MQ=255)
                                                                                  gcgcCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGCGTTGCGGGGG‑TGGG‑GGGGGCCACGA‑CAGTCCCCTTTTTTT‑TTATCGTGGGGATCATTTTTATTTTTTTTTTT‑TGCTAATGCAATAGTTACTGGAATGATCCGCTTAGTTTATGTTGAACaaa                                                                                                                                                                     >  2:453716/1‑250 (MQ=255)
                                                                                     ccAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAAtctc                                                                                                                                                                 <  1:143189/251‑1 (MQ=255)
                                                                                       aGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGc                                                                                                                                                                                                                    <  1:108543/198‑1 (MQ=255)
                                                                                            cGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGc                                                                                                                                                          <  1:18150/251‑1 (MQ=255)
                                                                                             gTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGGA‑TGGG‑GGCGGCCATGA‑CAGTCTCTTTTTTTT‑GTATCGTGGGAATCATTTTCATTTTTATTTTT‑AGCTTATGCAATAGTTACTGAACTTGTTCGATTAGTTTATGTTGAACAAATATCAAGtttgg                                                                                                                                                         >  1:313701/1‑248 (MQ=255)
                                                                                                  tGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGCCGCGGTTCGCGGGAA‑TGGG‑GGCGGCCCTGA‑CCGGCTCTTTTTTTT‑GTATCGTGGGAATACTTTTCATTTTTTTTTTT‑ATCTTATGTAATAGTTTATGAAATGAACCGATTGGTTTATTTTTAACAAATCTCATGTTGCgttgtg                                                                                                                                                    >  2:104129/1‑251 (MQ=255)
                                                                                                   gCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCCCGGTTCCCGGGGA‑TGGGGTG‑GGCCATGA‑CAGGCTCTTTTTTTTTGT‑TCGTGGGAATACTTTTCATTTTTATTTTT‑AGCTCATGCAAAAGTTACTGTACATATTCGCTGAGATAATGTTGAACAAAACTCACTTTGCGtggtgg                                                                                                                                                   >  1:415880/1‑251 (MQ=255)
                                                                                                     cacaCTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGGTTTGCGGGGA‑AGGG‑GGGGACCATTA‑CAGGCTCTTTTTTTT‑GTATCGTGGGAAACATTTTTCTTTTTTTTGTT‑AGGTTATGACATAGATACTGAAATGATCCGATGGGGTAATGTTGAAAAAAACTCATGTTGCGTgggggtg                                                                                                                                                 >  1:447717/1‑250 (MQ=255)
                                                                                                           gCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTCTGGCGCGGTTCGCGGGGA‑AGGG‑TGGGGCCATGAACA‑TTTCTTTTTTTT‑GTATCGTGGGAATAATTTTCATTTTTTTTTTT‑TGCTAATTCAATAGATACTGAAATGTTCCCATGAGGTAATGTTGTAAAAATATCATGTTGTGGGGTGGGGGC‑tttt                                                                                                                                          >  1:48801/1‑251 (MQ=255)
                                                                                                                 ggATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTTCCGCGTTTTCCGGGGA‑AGGG‑GGCGGCCCAGG‑CAGTCTCTTTTTTTT‑TTTTTGTGGGAAACATTTTCTTTTTTTTTTTT‑TGCTTATTCAAAAATTTCTTAACTGATCCGGTGGGTTTATGTTTTACAAATCTCCTGTTgtggggggggggctttttcccc                                                                                                                                      >  1:71623/1‑229 (MQ=255)
                                                                                                                  gATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTG‑AGCTAATTCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑g                                                                                                                                  <  1:409812/251‑1 (MQ=255)
                                                                                                                                  ccAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTTGCGGGAAGTGGG‑‑GCGGACACTA‑CAGGCTCTTTTTTTT‑TTATCGTGGGAATCATTTTTATTTTTTTTGTT‑TGCTAATGCAAAAGGTACTGAAATGATCCGATGAGGTTATATTGTAAAAAACTCAAGTTTCGGGGTGGGCTG‑TTTTACTACA‑AACGCGTTTATGCCAGt                                                                                                                  >  1:48424/1‑251 (MQ=255)
                                                                                                                                       aaTTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGGTTTTTGCCGCGTTTCGCGGGAA‑TGGG‑GGCGGCCCCGA‑CAGTCTCTTTTTTTT‑TTTTCGTGGGAATCATTTTCTTTTTTTTTTTTTAGTTATTCCATTA‑TTAATGAACTTATCCGGTGAGTTAATTTTGAACAAATTCCATGTTGGGTGGGGGTCGCCTTTTCCCAC‑‑GATGGGGTTATACCAGGATggg                                                                                                             >  2:407912/1‑250 (MQ=255)
                                                                                                                                          ttCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGttt                                                                                                           <  1:436044/250‑1 (MQ=255)
                                                                                                                                           tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTttg                                                                                                          <  1:162160/250‑1 (MQ=255)
                                                                                                                                           tCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTttg                                                                                                          <  2:113493/250‑1 (MQ=255)
                                                                                                                                                    gcgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTTTGCCGCGGTTTTCGGGGA‑TGGG‑GGGGAGCCCGA‑CAGTCTCTTTTTTCT‑TTATTGCGGGAATCCTTTTTATTTTTTTTGTT‑ATCTAATGCAATAGTTACTGAACTGTTCCGATGAGGTTATGTTTAACAAATCTCATTTTGTGGGGGGGGCGC‑TTTTAC‑ACAAGATTCGTTTATGTCAGGATGGGTTGTTGAAttttt                                                                                                >  1:7384/1‑251 (MQ=255)
                                                                                                                                                     cgTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTtta                                                                                               <  1:104129/251‑1 (MQ=255)
                                                                                                                                                                          aGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCgtgt                                                                         <  2:362001/251‑1 (MQ=255)
                                                                                                                                                                              gCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCggg                                                                     <  2:52505/251‑1 (MQ=255)
                                                                                                                                                                                         ggCAACGGCGGGGTTTCTGCCGGGGTTCTCGGGGA‑TGGG‑TGAGGCCCTGA‑CCGTCTCTTTTTTTT‑TTATCGTGGAAATCAATTTTATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAAATGATCCGATTGGTTAATGTTGAACAAATCTCCTGGTGCGGGGGGGGCGC‑TTTTTACACA‑GATGCGGTTTTGCCCGTATTGGTTGTTTAAGTTTTTTTAAATATGGGGTTGAG‑GTGTTGGGAggcgaggttt                                                          >  2:274142/1‑242 (MQ=255)
                                                                                                                                                                                          gCAACGGCGGGTTTTCTGCCGCGTTGCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTTGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTAGGCCAGTATGGTTTGTTTAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGag                                                         <  2:346236/251‑1 (MQ=255)
                                                                                                                                                                                                        tCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGaa                                            <  1:241262/250‑1 (MQ=255)
                                                                                                                                                                                                                        ggAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGtt                           <  2:313701/251‑1 (MQ=255)
                                                                                                                                                                                                                          aa‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaa                         <  1:40659/251‑1 (MQ=255)
                                                                                                                                                                                                                           a‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGGACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTaaa                        <  1:233901/251‑1 (MQ=255)
                                                                                                                                                                                                                                     gACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGt                 >  1:59517/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTg           <  2:437528/250‑1 (MQ=255)
                                                                                                                                                                                                                                                gTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCt      >  1:90057/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    ctTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAc  >  1:457963/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    ctTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCAGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAc  >  1:119584/1‑251 (MQ=255)
                                                                                                                                                                                                                                                      tttttCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGa   <  2:426247/248‑1 (MQ=255)
                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                        
TCGCCAACCTGACGCACGAAACCCTGCTGTTGCATCGTGGTTAGCAGGCGGTGGGTCGTGGAATTGGGTAACCCGGCTTGTTGCGCCAGTTCCGTGAGTGCCACACTGCCATTGGATTCGGCAATCCACTCCAGTAATTTCAGGCCACGCGTTAAAGACTGAACCTGTCCAGTCGCTGGTGCGGTGGCAACGGCGGGTTTTCTGCCGCGTTTCGCGGGAA‑TGGG‑TGCGACCATGA‑CAGTCTCTTTTTTCT‑GTATCGTGGAAATCATTTTCATTTTTATTGTT‑AGCTAATGCAATAGTTACTGAACTGATCCGATGAGTTAATGTTGAACAAATCTCATGTTGCGTGGTGGTCGC‑TTTTACCACA‑GATGCGTTTATGCCAGTATGGTTTGTTGAATTTTTATTAAATCTGGG‑TTGAGCGTGTCGGGAGCAAGTGTGAGCAGCAAAGTGGAACAACTGCGTGCGCAGTTAAATGAACGTATTCTGGTGCTGGAC  >  NC_000913/4223396‑4223887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑