breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC34.3% 25.6 / 27.4 35intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009133,743,6690GA31.7% 67.3 / 13.1 41E313K (GAA→AAA) yiaK2,3‑diketo‑L‑gulonate reductase
*NC_0009131,658,1670GT31.4% 79.2 / 15.2 52L33F (TTG→TTTynfEputative selenate reductase YnfE
*NC_0009134,604,3440CT30.8% 55.5 / 16.0 40S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009133,135,7810GT30.0% 82.3 / 14.4 51R197R (CGC→CGApitBmetal phosphate:H(+) symporter PitB
*NC_0009133,206,4350CT30.0% 57.5 / 10.2 40intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_0009132,833,5670TG29.9% 185.0 / 13.1 97G364G (GGT→GGGnorVanaerobic nitric oxide reductase flavorubredoxin
*NC_0009131,684,7360AC29.2% 76.1 / 10.4 49T160P (ACC→CCC) tusDNA replication terminus site‑binding protein
*NC_0009131,174,6050TC28.4% 177.9 / 14.0 81G187G (GGA→GGGycfTinner membrane protein YcfT
*NC_0009134,056,1150AC28.2% 134.9 / 10.5 72G75G (GGT→GGGglnLprotein histidine kinase NtrB
*NC_0009132,434,8820TG27.6% 156.0 / 11.8 77Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009134,332,7910AC27.3% 163.1 / 15.7 77V161G (GTG→GGG) basSsensor histidine kinase BasS
*NC_0009131,435,7270GA27.1% 99.9 / 17.2 49intergenic (‑108/+33)uspF/ompNuniversal stress protein F/outer membrane porin N
*NC_0009134,004,1320GA26.0% 101.4 / 10.0 51A16V (GCT→GTT) rarDputative transporter RarD
*NC_0009132,745,0890AC25.0% 173.3 / 12.3 81V84G (GTG→GGG) trmDtRNA m(1)G37 methyltransferase
*NC_0009134,275,9570AG25.0% 110.3 / 10.2 53S163G (AGT→GGT) pdeCc‑di‑GMP phosphodiesterase PdeC
*NC_0009132,185,1990TG24.3% 240.8 / 19.8 116T35P (ACC→CCC) thiMhydroxyethylthiazole kinase
*NC_0009132,878,9470CT23.8% 116.0 / 13.2 63E276K (GAA→AAA) cas1multifunctional nuclease Cas1
*NC_0009131,026,4400TG23.7% 200.1 / 10.1 77V657G (GTA→GGA) helDDNA helicase IV
*NC_0009132,337,7230AC23.6% 255.7 / 14.1 107G566G (GGT→GGGgyrADNA gyrase subunit A

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1411899 =59 (0.750)4 (0.060) 4/442 4.8 8.4% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 34 (0.480)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 552744107 (1.360)3 (0.040) 3/488 5.5 3.9% coding (357/510 nt) purE N(5)‑carboxyaminoimidazole ribonucleotide mutase
?NC_000913 1762399 = 44 (0.570)coding (114/747 nt) sufC Fe‑S cluster scaffold complex subunit SufC